ID X2DR70_9INFA Unreviewed; 757 AA. AC X2DR70; DT 11-JUN-2014, integrated into UniProtKB/TrEMBL. DT 11-JUN-2014, sequence version 1. DT 09-DEC-2015, entry version 10. DE RecName: Full=RNA-directed RNA polymerase catalytic subunit {ECO:0000256|RuleBase:RU004330, ECO:0000256|SAAS:SAAS00377010}; DE EC=2.7.7.48 {ECO:0000256|RuleBase:RU004330, ECO:0000256|SAAS:SAAS00376969}; GN Name=PB1 {ECO:0000313|EMBL:AHL81439.1}; OS Influenza A virus (A/Tennessee/F1080/2010(H1N1)). OC Viruses; ssRNA viruses; ssRNA negative-strand viruses; OC Orthomyxoviridae; Influenzavirus A. OX NCBI_TaxID=1452594 {ECO:0000313|EMBL:AHL81439.1}; RN [1] {ECO:0000313|EMBL:AHL81439.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=A/Tennessee/F1080/2010 {ECO:0000313|EMBL:AHL81439.1}; RA Wentworth D.E., Halpin R.A., Lin X., Bera J., Ransier A., Fedorova N., RA Tsitrin T., McLellan M., Stockwell T., Amedeo P., Appalla L., RA Bishop B., Edworthy P., Gupta N., Hoover J., Katzel D., Li K., RA Schobel S., Shrivastava S., Thovarai V., Wang S., Oshansky C.M., RA Wong S.S., Webby R., Bao Y., Sanders R., Zhdanov S., Kiryutin B., RA Lipman D.J., Tatusova T., Thomas P.G.; RT "The NIAID Influenza Genome Sequencing Project."; RL Submitted (MAR-2014) to the EMBL/GenBank/DDBJ databases. RN [2] {ECO:0000313|EMBL:AHL81439.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=A/Tennessee/F1080/2010 {ECO:0000313|EMBL:AHL81439.1}; RG The NIAID Influenza Genome Sequencing Consortium; RL Submitted (MAR-2014) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: RNA-dependent RNA polymerase which is responsible for CC replication and transcription of virus RNA segments. The CC transcription of viral mRNAs occurs by a unique mechanism called CC cap-snatching. 5' methylated caps of cellular mRNAs are cleaved CC after 10-13 nucleotides by PA. In turn, these short capped RNAs CC are used as primers by PB1 for transcription of viral mRNAs. CC During virus replication, PB1 initiates RNA synthesis and copy CC vRNA into complementary RNA (cRNA) which in turn serves as a CC template for the production of more vRNAs. CC {ECO:0000256|SAAS:SAAS00377137}. CC -!- CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate CC + RNA(n+1). {ECO:0000256|RuleBase:RU004330, CC ECO:0000256|SAAS:SAAS00376950}. CC -!- SUBUNIT: Influenza RNA polymerase is composed of three subunits: CC PB1, PB2 and PA. Interacts (via N-terminus) with PA (via C- CC terminus). Interacts (via C-terminus) with PB2 (via N-terminus); CC this interaction is essential for transcription initiation. CC {ECO:0000256|SAAS:SAAS00377302}. CC -!- SIMILARITY: Contains RdRp catalytic domain. CC {ECO:0000256|SAAS:SAAS00377245}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CY167786; AHL81439.1; -; Viral_cRNA. DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW. DR GO; GO:0003968; F:RNA-directed RNA polymerase activity; IEA:UniProtKB-KW. DR GO; GO:0039523; P:suppression by virus of host RNA polymerase II activity; IEA:UniProtKB-KW. DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro. DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro. DR GO; GO:0019083; P:viral transcription; IEA:UniProtKB-KW. DR InterPro; IPR007099; RNA-dir_pol_NSvirus. DR InterPro; IPR001407; RNA_pol_PB1_influenza. DR Pfam; PF00602; Flu_PB1; 1. DR PIRSF; PIRSF000827; RdRPol_OMV; 1. DR PROSITE; PS50525; RDRP_SSRNA_NEG_SEG; 1. PE 4: Predicted; KW Eukaryotic host gene expression shutoff by virus KW {ECO:0000256|SAAS:SAAS00239605}; KW Eukaryotic host transcription shutoff by virus KW {ECO:0000256|SAAS:SAAS00239775}; KW Host gene expression shutoff by virus {ECO:0000256|SAAS:SAAS00239728}; KW Host-virus interaction {ECO:0000256|SAAS:SAAS00239786}; KW Inhibition of host RNA polymerase II by virus KW {ECO:0000256|SAAS:SAAS00239606}; KW Nucleotide-binding {ECO:0000256|SAAS:SAAS00276578}; KW Nucleotidyltransferase {ECO:0000256|SAAS:SAAS00276602}; KW RNA-directed RNA polymerase {ECO:0000256|RuleBase:RU004330, KW ECO:0000256|SAAS:SAAS00276589}; KW Transferase {ECO:0000256|SAAS:SAAS00276586}; KW Viral RNA replication {ECO:0000256|SAAS:SAAS00276580}; KW Viral transcription {ECO:0000256|SAAS:SAAS00239449}. FT DOMAIN 286 483 RdRp catalytic. {ECO:0000259|PROSITE: FT PS50525}. SQ SEQUENCE 757 AA; 86323 MW; E99C4D5A768A9223 CRC64; MDVNPTLLFL KIPAQNAIST TFPYTGDPPY SHGTGTGYTM DTVNRTHQYS EKGKWTTNTE TGAPQLNPID GPLPEDNEPS GYAQTDCVLE AMAFLEESHP GIFENSCLET MEVVQQTRVD KLTQGRQTYD WTLNRNQPAA TALANTIEVF RSNGLTANES GRLIDFLKDV MESMNKEEIE ITTHFQRKRR VRDNMTKKMV TQRTIGKKKQ RLNKRGYLIR ALTLNTMTKD AERGKLKRRA IATPGMQIRG FVYFVETLAR SICEKLEQSG LPVGGNEKKA KLANVVRKMM TNSQDTEISF TITGDNTKWN ENQNPRMFLA MITYITRNQP EWFRNILSMA PIMFSNKMAR LGKGYMFESK RMKIRTQIPA EMLASIDLKY FNESTKKKIE KIRPLLIDGT ASLSPGMMMG MFNMLSTVLG VSILNLGQKK YTKTIYWWDG LQSSDDFALI VNAPNHEGIQ AGVDRFYRTC KLVGINMSKK KSYINKTGTF EFTSFFYRYG FVANFSMELP SFGVSGVNES ADMSIGVTVI KNNMINNDLG PATAQMALQL FIKDYRYTYR CHRGDTQIQT RRSFELKKLW DQTQSKVGLL VSDGGPNLYN IRNLHIPEVC LKWELMDDDY RGRLCNPLNP FVSHKEIDSV NNAVVMPAHG PAKSMEYDAV ATTHSWIPKR NRSILNTSQR GILEDEQMYQ KCCNLFEKFF PSSSYRRPVG ISSMVEAMVS RARIDARVDF ESGRIGKEEF SEIMKICSTI EELRRQK //