ID V9WZ98_9PSED Unreviewed; 305 AA. AC V9WZ98; DT 19-MAR-2014, integrated into UniProtKB/TrEMBL. DT 19-MAR-2014, sequence version 1. DT 29-SEP-2021, entry version 41. DE RecName: Full=tRNA pseudouridine synthase B {ECO:0000256|HAMAP-Rule:MF_01080}; DE EC=5.4.99.25 {ECO:0000256|HAMAP-Rule:MF_01080}; DE AltName: Full=tRNA pseudouridine(55) synthase {ECO:0000256|HAMAP-Rule:MF_01080}; DE Short=Psi55 synthase {ECO:0000256|HAMAP-Rule:MF_01080}; DE AltName: Full=tRNA pseudouridylate synthase {ECO:0000256|HAMAP-Rule:MF_01080}; DE AltName: Full=tRNA-uridine isomerase {ECO:0000256|HAMAP-Rule:MF_01080}; GN Name=truB {ECO:0000256|HAMAP-Rule:MF_01080}; GN ORFNames=C163_22910 {ECO:0000313|EMBL:AHD16486.1}; OS Pseudomonas sp. FGI182. OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; OC Pseudomonadaceae; Pseudomonas. OX NCBI_TaxID=1259844 {ECO:0000313|EMBL:AHD16486.1, ECO:0000313|Proteomes:UP000018783}; RN [1] {ECO:0000313|EMBL:AHD16486.1, ECO:0000313|Proteomes:UP000018783} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=FGI182 {ECO:0000313|EMBL:AHD16486.1, RC ECO:0000313|Proteomes:UP000018783}; RG DOE Joint Genome Institute; RA Currie C., Huntemann M., Han J., Chen A., Kyrpides N., Mavromatis K., RA Markowitz V., Palaniappan K., Ivanova N., Schaumberg A., Pati A., RA Liolios K., Nordberg H.P., Cantor M.N., Hua S.X., Woyke T.; RL Submitted (DEC-2013) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-55 in CC the psi GC loop of transfer RNAs. {ECO:0000256|HAMAP-Rule:MF_01080}. CC -!- CATALYTIC ACTIVITY: CC Reaction=uridine(55) in tRNA = pseudouridine(55) in tRNA; CC Xref=Rhea:RHEA:42532, Rhea:RHEA-COMP:10101, Rhea:RHEA-COMP:10102, CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.25; CC Evidence={ECO:0000256|ARBA:ARBA00000385, ECO:0000256|HAMAP- CC Rule:MF_01080}; CC -!- SIMILARITY: Belongs to the pseudouridine synthase TruB family. Type 1 CC subfamily. {ECO:0000256|ARBA:ARBA00005642, ECO:0000256|HAMAP- CC Rule:MF_01080}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP007012; AHD16486.1; -; Genomic_DNA. DR RefSeq; WP_013974284.1; NZ_CP007012.1. DR EnsemblBacteria; AHD16486; AHD16486; C163_22910. DR GeneID; 58534570; -. DR PATRIC; fig|1259844.3.peg.4506; -. DR HOGENOM; CLU_032087_0_3_6; -. DR Proteomes; UP000018783; Chromosome. DR GO; GO:0003723; F:RNA binding; IEA:InterPro. DR GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC. DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule. DR CDD; cd02573; PseudoU_synth_EcTruB; 1. DR Gene3D; 2.30.130.10; -; 1. DR HAMAP; MF_01080; TruB_bact; 1. DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf. DR InterPro; IPR002501; PsdUridine_synth_N. DR InterPro; IPR015947; PUA-like_sf. DR InterPro; IPR036974; PUA_sf. DR InterPro; IPR014780; tRNA_psdUridine_synth_TruB. DR InterPro; IPR015240; tRNA_sdUridine_synth_fam1_C. DR InterPro; IPR032819; TruB_C. DR PANTHER; PTHR13767; PTHR13767; 1. DR Pfam; PF09157; TruB-C_2; 1. DR Pfam; PF16198; TruB_C_2; 1. DR Pfam; PF01509; TruB_N; 1. DR SUPFAM; SSF55120; SSF55120; 1. DR SUPFAM; SSF88697; SSF88697; 1. DR TIGRFAMs; TIGR00431; TruB; 1. PE 3: Inferred from homology; KW Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000256|HAMAP-Rule:MF_01080}; KW tRNA processing {ECO:0000256|HAMAP-Rule:MF_01080}. FT DOMAIN 33..180 FT /note="TruB_N" FT /evidence="ECO:0000259|Pfam:PF01509" FT DOMAIN 181..242 FT /note="TruB_C_2" FT /evidence="ECO:0000259|Pfam:PF16198" FT DOMAIN 246..302 FT /note="TruB-C_2" FT /evidence="ECO:0000259|Pfam:PF09157" FT ACT_SITE 48 FT /note="Nucleophile" FT /evidence="ECO:0000256|HAMAP-Rule:MF_01080" SQ SEQUENCE 305 AA; 33453 MW; 808F3EB8CD55E1FB CRC64; MAQVKRIRRN VSGIILLDKP LGFTSNAALQ KVRWLLNAEK AGHTGSLDPL ATGVLPLCFG EATKFSQYLL DSDKGYETVM QMGQTTNTGD AEGEVLQTRE VTVGRADIEA LLPRFRGQIS QIPPMYSALK RDGQPLYKLA RAGEVVEREA RSVTIGRLEL LECEGTRARL SVGCSKGTYI RTLVEDIGEA LGCGAYVAEL RRTQAGPFAL AQTVTLEELE QAHAEGGNEA LDRFLMPSDS GLQDWPLVCL SEHSAFYWLH GQAVRAPDAP QFGMVRVQDH NARFIGIGEV SEDGRIAPRR LIRSE //