ID V9WZ98_9PSED Unreviewed; 305 AA. AC V9WZ98; DT 19-MAR-2014, integrated into UniProtKB/TrEMBL. DT 19-MAR-2014, sequence version 1. DT 27-SEP-2017, entry version 25. DE RecName: Full=tRNA pseudouridine synthase B {ECO:0000256|HAMAP-Rule:MF_01080}; DE EC=5.4.99.25 {ECO:0000256|HAMAP-Rule:MF_01080}; DE AltName: Full=tRNA pseudouridine(55) synthase {ECO:0000256|HAMAP-Rule:MF_01080}; DE Short=Psi55 synthase {ECO:0000256|HAMAP-Rule:MF_01080}; DE AltName: Full=tRNA pseudouridylate synthase {ECO:0000256|HAMAP-Rule:MF_01080}; DE AltName: Full=tRNA-uridine isomerase {ECO:0000256|HAMAP-Rule:MF_01080}; GN Name=truB {ECO:0000256|HAMAP-Rule:MF_01080}; GN ORFNames=C163_22910 {ECO:0000313|EMBL:AHD16486.1}; OS Pseudomonas sp. FGI182. OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; OC Pseudomonadaceae; Pseudomonas. OX NCBI_TaxID=1259844 {ECO:0000313|EMBL:AHD16486.1, ECO:0000313|Proteomes:UP000018783}; RN [1] {ECO:0000313|EMBL:AHD16486.1, ECO:0000313|Proteomes:UP000018783} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=FGI182 {ECO:0000313|EMBL:AHD16486.1, RC ECO:0000313|Proteomes:UP000018783}; RG DOE Joint Genome Institute; RA Currie C., Huntemann M., Han J., Chen A., Kyrpides N., Mavromatis K., RA Markowitz V., Palaniappan K., Ivanova N., Schaumberg A., Pati A., RA Liolios K., Nordberg H.P., Cantor M.N., Hua S.X., Woyke T.; RL Submitted (DEC-2013) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil- CC 55 in the psi GC loop of transfer RNAs. {ECO:0000256|HAMAP- CC Rule:MF_01080, ECO:0000256|SAAS:SAAS00534152}. CC -!- CATALYTIC ACTIVITY: tRNA uridine(55) = tRNA pseudouridine(55). CC {ECO:0000256|HAMAP-Rule:MF_01080, ECO:0000256|SAAS:SAAS00415305}. CC -!- SIMILARITY: Belongs to the pseudouridine synthase TruB family. CC Type 1 subfamily. {ECO:0000256|HAMAP-Rule:MF_01080, CC ECO:0000256|SAAS:SAAS00534157}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP007012; AHD16486.1; -; Genomic_DNA. DR RefSeq; WP_013974284.1; NZ_CP007012.1. DR EnsemblBacteria; AHD16486; AHD16486; C163_22910. DR PATRIC; fig|1259844.3.peg.4506; -. DR Proteomes; UP000018783; Chromosome. DR GO; GO:0009982; F:pseudouridine synthase activity; IEA:UniProtKB-UniRule. DR GO; GO:0003723; F:RNA binding; IEA:InterPro. DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule. DR Gene3D; 2.30.130.10; -; 1. DR HAMAP; MF_01080; TruB_bact; 1. DR InterPro; IPR020103; PsdUridine_synth_cat_dom. DR InterPro; IPR002501; PsdUridine_synth_N. DR InterPro; IPR002478; PUA. DR InterPro; IPR015947; PUA-like_domain. DR InterPro; IPR014780; tRNA_psdUridine_synth_TruB. DR InterPro; IPR015240; tRNA_sdUridine_synth_fam1_C. DR InterPro; IPR032819; TruB_C. DR PANTHER; PTHR13767; PTHR13767; 1. DR Pfam; PF09157; TruB-C_2; 1. DR Pfam; PF16198; TruB_C_2; 1. DR Pfam; PF01509; TruB_N; 1. DR SUPFAM; SSF55120; SSF55120; 1. DR SUPFAM; SSF88697; SSF88697; 1. DR TIGRFAMs; TIGR00431; TruB; 1. PE 3: Inferred from homology; KW Complete proteome {ECO:0000313|Proteomes:UP000018783}; KW Isomerase {ECO:0000256|HAMAP-Rule:MF_01080, KW ECO:0000256|SAAS:SAAS00103062}; KW tRNA processing {ECO:0000256|HAMAP-Rule:MF_01080, KW ECO:0000256|SAAS:SAAS00103090}. FT DOMAIN 33 180 TruB_N. {ECO:0000259|Pfam:PF01509}. FT DOMAIN 181 242 TruB_C_2. {ECO:0000259|Pfam:PF16198}. FT DOMAIN 246 302 TruB-C_2. {ECO:0000259|Pfam:PF09157}. FT ACT_SITE 48 48 Nucleophile. {ECO:0000256|HAMAP-Rule: FT MF_01080}. SQ SEQUENCE 305 AA; 33453 MW; 808F3EB8CD55E1FB CRC64; MAQVKRIRRN VSGIILLDKP LGFTSNAALQ KVRWLLNAEK AGHTGSLDPL ATGVLPLCFG EATKFSQYLL DSDKGYETVM QMGQTTNTGD AEGEVLQTRE VTVGRADIEA LLPRFRGQIS QIPPMYSALK RDGQPLYKLA RAGEVVEREA RSVTIGRLEL LECEGTRARL SVGCSKGTYI RTLVEDIGEA LGCGAYVAEL RRTQAGPFAL AQTVTLEELE QAHAEGGNEA LDRFLMPSDS GLQDWPLVCL SEHSAFYWLH GQAVRAPDAP QFGMVRVQDH NARFIGIGEV SEDGRIAPRR LIRSE //