ID   V9TVB2_MERS             Unreviewed;      2685 AA.
AC   V9TVB2;
DT   19-MAR-2014, integrated into UniProtKB/TrEMBL.
DT   19-MAR-2014, sequence version 1.
DT   27-NOV-2024, entry version 63.
DE   RecName: Full=ORF1ab polyprotein {ECO:0000256|PROSITE-ProRule:PRU01344};
DE   Flags: Fragment;
GN   Name=orf1ab {ECO:0000313|EMBL:AHC74097.1};
OS   Middle East respiratory syndrome-related coronavirus (MERS-CoV).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC   Betacoronavirus; Merbecovirus.
OX   NCBI_TaxID=1335626 {ECO:0000313|EMBL:AHC74097.1, ECO:0000313|Proteomes:UP000123963};
RN   [1] {ECO:0000313|EMBL:AHC74097.1, ECO:0000313|Proteomes:UP000123963}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Qatar4 {ECO:0000313|EMBL:AHC74097.1};
RA   Haagmans B.L., Al-Dhahiry S.H.S., Reusken C.B.E.M., Raj V.S., Galiano M.,
RA   Myers R., Godeke G.-J., Jonges M., Farag E., Diab A., Ghobashy H.,
RA   Alhajri F., Al-Thani M., Al-Marri S.A., Al Romaihi H.E., Al Khal A.,
RA   Bermingham A., Osterhaus A.D.M.E., AlHajri M.M., Koopmans M.P.G.;
RT   "Middle East respiratory syndrome coronavirus in dromedary camels: an
RT   outbreak investigation.";
RL   Lancet Infect. Dis. 0:0-0(2013).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + phosphate + H(+); Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC         Evidence={ECO:0000256|ARBA:ARBA00001665};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + phosphate + H(+); Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC         Evidence={ECO:0000256|ARBA:ARBA00001556};
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm, host perinuclear region
CC       {ECO:0000256|ARBA:ARBA00004407}. Host membrane
CC       {ECO:0000256|ARBA:ARBA00004301}; Multi-pass membrane protein
CC       {ECO:0000256|ARBA:ARBA00004301}.
CC   -!- SIMILARITY: Belongs to the coronaviruses polyprotein 1ab family.
CC       {ECO:0000256|ARBA:ARBA00008087, ECO:0000256|PROSITE-ProRule:PRU01299}.
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DR   EMBL; KF961222; AHC74097.1; -; Genomic_RNA.
DR   Proteomes; UP000123963; Genome.
DR   GO; GO:0044220; C:host cell perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0000175; F:3'-5'-RNA exonuclease activity; IEA:InterPro.
DR   GO; GO:0043139; F:5'-3' DNA helicase activity; IEA:TreeGrafter.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0008234; F:cysteine-type peptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0004483; F:mRNA (nucleoside-2'-O-)-methyltransferase activity; IEA:InterPro.
DR   GO; GO:0004482; F:mRNA 5'-cap (guanine-N7-)-methyltransferase activity; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   GO; GO:0075523; P:viral translational frameshifting; IEA:UniProtKB-KW.
DR   CDD; cd21409; 1B_cv_Nsp13-like; 1.
DR   CDD; cd21722; betaCoV_Nsp13-helicase; 1.
DR   CDD; cd21659; betaCoV_Nsp14; 1.
DR   CDD; cd20762; capping_2-OMTase_Nidovirales; 1.
DR   CDD; cd21167; M_alpha_beta_cv_Nsp15-like; 1.
DR   CDD; cd21158; NendoU_nv; 1.
DR   CDD; cd21171; NTD_alpha_betaCoV_Nsp15-like; 1.
DR   CDD; cd21689; stalk_CoV_Nsp13-like; 1.
DR   CDD; cd21401; ZBD_cv_Nsp13-like; 1.
DR   FunFam; 3.40.50.150:FF:000162; Orf1ab polyprotein; 1.
DR   FunFam; 3.40.50.300:FF:001105; Orf1ab polyprotein; 1.
DR   FunFam; 3.40.50.300:FF:001139; Orf1ab polyprotein; 1.
DR   Gene3D; 3.40.50.11580; -; 1.
DR   Gene3D; 3.30.160.820; Nsp15 N-terminal domain-like; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR   InterPro; IPR027351; (+)RNA_virus_helicase_core_dom.
DR   InterPro; IPR046440; AV_NSP11N_COV_NSP15M.
DR   InterPro; IPR050534; Coronavir_polyprotein_1ab.
DR   InterPro; IPR043608; CoV_NSP15_M.
DR   InterPro; IPR043606; CoV_NSP15_N.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR041679; DNA2/NAM7-like_C.
DR   InterPro; IPR037227; EndoU-like.
DR   InterPro; IPR046435; N7_MTase_CoV.
DR   InterPro; IPR043609; NendoU_nidovirus.
DR   InterPro; IPR044863; NIRAN.
DR   InterPro; IPR046438; NIV_2_O_MTASE.
DR   InterPro; IPR046436; NIV_EXON.
DR   InterPro; IPR047570; NSP12_IF_CoV.
DR   InterPro; IPR044343; NSP13_1B_dom_CoV.
DR   InterPro; IPR048673; NSP13_stalk_CoV.
DR   InterPro; IPR048672; NSP13_ZBD_CoV.
DR   InterPro; IPR027352; NSP13_ZBD_CoV-like.
DR   InterPro; IPR044315; NSP14_betaCoV.
DR   InterPro; IPR009466; NSP14_CoV.
DR   InterPro; IPR044330; NSP15_alpha_betaCoV_N.
DR   InterPro; IPR044322; NSP15_M_alpha_beta_CoV.
DR   InterPro; IPR043174; NSP15_middle_sf.
DR   InterPro; IPR042515; NSP15_N_CoV.
DR   InterPro; IPR009461; NSP16_CoV-like.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR046441; RdRp_CoV.
DR   InterPro; IPR009469; RdRp_N_CoV.
DR   InterPro; IPR001205; RNA-dir_pol_C.
DR   InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PANTHER; PTHR43788; DNA2/NAM7 HELICASE FAMILY MEMBER; 1.
DR   PANTHER; PTHR43788:SF8; HELICASE WITH ZINC FINGER 2; 1.
DR   Pfam; PF13087; AAA_12; 1.
DR   Pfam; PF13604; AAA_30; 1.
DR   Pfam; PF06471; CoV_ExoN; 1.
DR   Pfam; PF06460; CoV_Methyltr_2; 1.
DR   Pfam; PF20631; CoV_NSP13_1B; 1.
DR   Pfam; PF20633; CoV_NSP13_stalk; 1.
DR   Pfam; PF20632; CoV_NSP13_ZBD; 1.
DR   Pfam; PF19215; CoV_NSP15_C; 1.
DR   Pfam; PF19216; CoV_NSP15_M; 1.
DR   Pfam; PF19219; CoV_NSP15_N; 1.
DR   Pfam; PF06478; CoV_RPol_N; 1.
DR   Pfam; PF00680; RdRP_1; 1.
DR   SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR   SUPFAM; SSF142877; EndoU-like; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR   PROSITE; PS51961; AV_NSP11N_COV_NSP15M; 1.
DR   PROSITE; PS51954; COV_N7_MTASE; 1.
DR   PROSITE; PS52000; COV_NSP12_IF; 1.
DR   PROSITE; PS51948; COV_NSP12_RDRP; 1.
DR   PROSITE; PS51960; COV_NSP15_NTD; 1.
DR   PROSITE; PS51653; CV_ZBD; 1.
DR   PROSITE; PS51958; NENDOU; 1.
DR   PROSITE; PS51947; NIRAN; 1.
DR   PROSITE; PS51955; NIV_2_O_MTASE; 1.
DR   PROSITE; PS51953; NIV_EXON; 1.
DR   PROSITE; PS51657; PSRV_HELICASE; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   3: Inferred from homology;
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW   Endonuclease {ECO:0000256|ARBA:ARBA00022759, ECO:0000256|PROSITE-
KW   ProRule:PRU01303};
KW   Exonuclease {ECO:0000256|ARBA:ARBA00022839, ECO:0000256|PROSITE-
KW   ProRule:PRU01298}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW   Host cytoplasm {ECO:0000256|ARBA:ARBA00023200};
KW   Host membrane {ECO:0000256|ARBA:ARBA00022870};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW   ProRule:PRU01303}; Membrane {ECO:0000256|ARBA:ARBA00023136};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW   ProRule:PRU01299};
KW   Nuclease {ECO:0000256|ARBA:ARBA00022722, ECO:0000256|PROSITE-
KW   ProRule:PRU01298}; Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW   Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW   Protease {ECO:0000256|ARBA:ARBA00022807};
KW   Ribosomal frameshifting {ECO:0000256|ARBA:ARBA00022758};
KW   RNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022484};
KW   Thiol protease {ECO:0000256|ARBA:ARBA00022807};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW   ProRule:PRU01299}; Transmembrane {ECO:0000256|ARBA:ARBA00022692};
KW   Transmembrane helix {ECO:0000256|ARBA:ARBA00022989};
KW   Viral RNA replication {ECO:0000256|ARBA:ARBA00022953};
KW   Zinc {ECO:0000256|ARBA:ARBA00022833};
KW   Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW   ProRule:PRU00986}.
FT   DOMAIN          1..246
FT                   /note="NiRAN"
FT                   /evidence="ECO:0000259|PROSITE:PS51947"
FT   DOMAIN          251..349
FT                   /note="Nsp12 Interface"
FT                   /evidence="ECO:0000259|PROSITE:PS52000"
FT   DOMAIN          350..917
FT                   /note="Nsp12 RNA-dependent RNA polymerase"
FT                   /evidence="ECO:0000259|PROSITE:PS51948"
FT   DOMAIN          597..759
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000259|PROSITE:PS50507"
FT   DOMAIN          918..1001
FT                   /note="CV ZBD"
FT                   /evidence="ECO:0000259|PROSITE:PS51653"
FT   DOMAIN          1174..1530
FT                   /note="(+)RNA virus helicase C-terminal"
FT                   /evidence="ECO:0000259|PROSITE:PS51657"
FT   DOMAIN          1587..1802
FT                   /note="ExoN"
FT                   /evidence="ECO:0000259|PROSITE:PS51953"
FT   DOMAIN          1811..2039
FT                   /note="N7-MTase"
FT                   /evidence="ECO:0000259|PROSITE:PS51954"
FT   DOMAIN          2040..2100
FT                   /note="Nsp15 N-terminal oligomerization"
FT                   /evidence="ECO:0000259|PROSITE:PS51960"
FT   DOMAIN          2101..2223
FT                   /note="AV-Nsp11N/CoV-Nsp15M"
FT                   /evidence="ECO:0000259|PROSITE:PS51961"
FT   DOMAIN          2240..2379
FT                   /note="NendoU"
FT                   /evidence="ECO:0000259|PROSITE:PS51958"
FT   DOMAIN          2384..2678
FT                   /note="Nidovirus-type SAM-dependent 2'-O-MTase"
FT                   /evidence="ECO:0000259|PROSITE:PS51955"
FT   REGION          1925..1939
FT                   /note="GpppA-binding"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01299"
FT   ACT_SITE        1605
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01298"
FT   ACT_SITE        1607
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01298"
FT   ACT_SITE        1706
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01298"
FT   ACT_SITE        1783
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01298"
FT   ACT_SITE        1788
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01298"
FT   ACT_SITE        2270
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01303"
FT   ACT_SITE        2285
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01303"
FT   ACT_SITE        2325
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01303"
FT   BINDING         1846..1852
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01299"
FT   NON_TER         1
FT                   /evidence="ECO:0000313|EMBL:AHC74097.1"
SQ   SEQUENCE   2685 AA;  303219 MW;  7638CB81574474A1 CRC64;
     ARIEPCSSGL STDVVFRAFD ICNYKAKVAG IGKYYKTNTC RFVELDDQGH HLDSYFVVKR
     HTMENYELEK HCYDLLRDCD AVAPHDFFIF DVDKVKTPHI VRQRLTEYTM MDLVYALRHF
     DQNSEVLKAI LVKYGCCDVT YFENKLWFDF VENPSVIGVY HKLGERVRQA ILNTVKFCDH
     MVKAGLVGVL TLDNQDLNGK WYDFGDFVIT QPGSGVAIVD SYYSYLMPVL SMTDCLAAET
     HRDCDFNKPL IEWPLTEYDF TDYKVQLFEK YFKYWDQTYH ANCVNCTDDR CVLHCANFNV
     LFAMTMPKTC FGPIVRKIFV DGVPFVVSCG YHYKELGLVM NMDVSLHRHR LSLKELMMYA
     ADPAMHIASS NAFLDLRTSC FSVAALTTGL TFQTVRPGNF NQDFYDFVVS KGFFKEGSSV
     TLKHFFFAQD GNAAITDYNY YSYNLPTMCD IKQMLFCMEV VNKYFEIYDG GCLNASEVVV
     NNLDKSAGHP FNKFGKARVY YESMSYQEQD ELFAMTKRNV IPTMTQMNLK YAISAKNRAR
     TVAGVSILST MTNRQYHQKM LKSMAATRGA TCVIGTTKFY GGWDFMLKTL YKDVDNPHLM
     GWDYPKCDRA MPNMCRIFAS LILARKHGTC CTTRDRFYRL ANECAQVLSE YVLCGGGYYV
     KPGGTSSGDA TTAYANSVFN ILQATTANVS ALMGANGNKI VDKEVKDMQF DLYVNVYRST
     SPDPKFVDKY YAFLNKHFSM MILSDDGVVC YNSDYAAKGY IAGIQNFKET LYYQNNVFMS
     EAKCWVETDL KKGPHEFCSQ HTLYIKDGDD GYFLPYPDPS RILSAGCFVD DIVKTDGTLM
     VERFVSLAID AYPLTKHEDI EYQNVFWVYL QYIEKLYKDL TGHMLDSYSV MLCGDNSAKF
     WEEAFYRDLY SSPTTLQAVG SCVVCHSQTS LRCGTCIRRP FLCCKCCYDH VIATPHKMVL
     SVSPYVCNAP GCGVSDVTKL YLGGMSYFCV DHRPVCSFPL CANGLVFGLY KNMCTGSPSI
     VEFNRLATCD WTESGDYTLA NTTTEPLKLF AAETLRATEE ASKQSYAIAT IKEIVGERQL
     LLVWEAGKSK PPLNRNYVFT GYHITKNSKV QLGEYIFERI DYSDAVSYKS STTYKLTVGD
     IFVLTSHSVA TLTAPTIVNQ ERYVKITGLY PTITVPEEFA SHVANFQKSG YSKYVTVQGP
     PGTGKSHFAI GLAIYYPTAR VVYTACSHAA VDALCEKAFK YLNIAKCSRI IPAKARVECY
     DRFKVNETNS QYLFSTINAL PETSADILVV DEVSMCTNYD LSIINARIKA KHIVYVGDPA
     QLPAPRTLLT RGTLEPENFN SVTRLMCNLG PDIFLSMCYR CPKEIVSTVS ALVYNNKLLA
     KKELSGQCFK ILYKGNVTHD ASSAINRPQL TFVKNFITAN PAWSKAVFIS PYNSQNAVAR
     SMLGLTTQTV DSSQGSEYQY VIFCQTADTA HANNINRFNV AITRAQKGIL CVMTSQALFE
     SLEFTELSFT NYKLQSQIVT GLFKDCSRET SGLSPAYAPT YVSVDDKYKT SDELCVNLNL
     PANVPYSRVI SRMGFKLDAT VPGYPKLFIT REEAVRQVRS WIGFDVEGAH ASRNACGTNV
     PLQLGFSTGV NFVVQPVGVV DTEWGNMLTG IAARPPPGEQ FKHLVPLMHK GAAWPIVRRR
     IVQMLSDTLD KLSDYCTFVC WAHGFELTSA SYFCKIGKEQ KCCMCNRRAA AYSSPLQSYA
     CWTHSCGYDY VYNPFFVDVQ QWGYVGNLAT NHDRYCSVHQ GAHVASNDAI MTRCLAIHSC
     FIERVDWDIE YPYISHEKKL NSCCRIVERN VVRAALLAGS FDKVYDIGNP KGIPIVDDPV
     VDWHYFDAQP LTRKVQQLFY TEDMASRFAD GLCLFWNCNV PKYPNNAIVC RFDTRVHSEF
     NLPGCDGGSL YVNKHAFHTP AYDVSAFRDL KPLPFFYYST TPCEVHGNGS MIEDIDYVPL
     KSAVCITTCN LGGAVCRKHA TEYREYMEAY NLVSASGFRL WCYKTFDIYN LWSTFTKVQG
     LENIAFNVVK QGHFIGVEGE LPVAVVNDKI FTKSGVNDIC MFENKTTLPT NIAFELYAKR
     AVRSHPDFKL LHNLQADICY KFVLWDYERS NIYGTATIGV CKYTDIDVNS ALNICFDIRD
     NGSLEKFMST PNAIFISDRK IKKYPCMVGP DYAYFNGAII RDSDVVKQPV KFYLYKKVNN
     EFIDPTECIY TQSRSCSDFL PLSDMEKDFL SFDSDVFIKK YGLENYAFEH VVYGDFSHTT
     LGGLHLLIGL YKKQQEGHII MEEMLKGSST IHNYFITETN TAAFKAVCSV IDLKLDDFVM
     ILKSQDLGVV SKVVKVPIDL TMIEFMLWCK DGQVQTFYPR LQASADWKPG HAMPSLFKVQ
     NVNLERCELA NYKQSIPMPR GVHMNIAKYM QLCQYLNTCT LAVPANMRVI HFGAGSDKGI
     APGTSVLRQW LPIDAIIIDN DLNEFVSDAD ITLFGDCVTV RVGQQVDLVI SDMYDPTTKN
     VTGSNESKAL FFTYLCNLIN NNLALGGSVA IKITEHSWSV ELYELMGKFA WWTVFCTNAN
     ASSSEGFLLG INYLGTIKEN IDGGAMHANY IFWRNSTPMN LSTYSLFDLS KFQLKLKGTP
     VLQLKESQIN ELVISLLSQG KLLIRDNDTL SVSTDVLVNT YRKLR
//