ID V9TVB2_MERS Unreviewed; 2685 AA. AC V9TVB2; DT 19-MAR-2014, integrated into UniProtKB/TrEMBL. DT 19-MAR-2014, sequence version 1. DT 24-JUL-2024, entry version 61. DE RecName: Full=ORF1ab polyprotein {ECO:0000256|PROSITE-ProRule:PRU01344}; DE Flags: Fragment; GN Name=orf1ab {ECO:0000313|EMBL:AHC74097.1}; OS Middle East respiratory syndrome-related coronavirus (MERS-CoV). OC Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; OC Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; OC Betacoronavirus; Merbecovirus. OX NCBI_TaxID=1335626 {ECO:0000313|EMBL:AHC74097.1, ECO:0000313|Proteomes:UP000123963}; RN [1] {ECO:0000313|EMBL:AHC74097.1, ECO:0000313|Proteomes:UP000123963} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Qatar4 {ECO:0000313|EMBL:AHC74097.1}; RA Haagmans B.L., Al-Dhahiry S.H.S., Reusken C.B.E.M., Raj V.S., Galiano M., RA Myers R., Godeke G.-J., Jonges M., Farag E., Diab A., Ghobashy H., RA Alhajri F., Al-Thani M., Al-Marri S.A., Al Romaihi H.E., Al Khal A., RA Bermingham A., Osterhaus A.D.M.E., AlHajri M.M., Koopmans M.P.G.; RT "Middle East respiratory syndrome coronavirus in dromedary camels: an RT outbreak investigation."; RL Lancet Infect. Dis. 0:0-0(2013). CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13; CC Evidence={ECO:0000256|ARBA:ARBA00001556}; CC -!- SUBCELLULAR LOCATION: Host cytoplasm, host perinuclear region CC {ECO:0000256|ARBA:ARBA00004407}. Membrane CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein CC {ECO:0000256|ARBA:ARBA00004141}. CC -!- SIMILARITY: Belongs to the coronaviruses polyprotein 1ab family. CC {ECO:0000256|ARBA:ARBA00008087, ECO:0000256|PROSITE-ProRule:PRU01299}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; KF961222; AHC74097.1; -; Genomic_RNA. DR Proteomes; UP000123963; Genome. DR GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-KW. DR GO; GO:0044220; C:host cell perinuclear region of cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell. DR GO; GO:0000175; F:3'-5'-RNA exonuclease activity; IEA:InterPro. DR GO; GO:0043139; F:5'-3' DNA helicase activity; IEA:TreeGrafter. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-UniRule. DR GO; GO:0004483; F:mRNA (nucleoside-2'-O-)-methyltransferase activity; IEA:InterPro. DR GO; GO:0004482; F:mRNA 5'-cap (guanine-N7-)-methyltransferase activity; IEA:InterPro. DR GO; GO:0003723; F:RNA binding; IEA:InterPro. DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC. DR GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:UniProtKB-KW. DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro. DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro. DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro. DR CDD; cd21409; 1B_cv_Nsp13-like; 1. DR CDD; cd21722; betaCoV_Nsp13-helicase; 1. DR CDD; cd21659; betaCoV_Nsp14; 1. DR CDD; cd20762; capping_2-OMTase_Nidovirales; 1. DR CDD; cd21167; M_alpha_beta_cv_Nsp15-like; 1. DR CDD; cd21158; NendoU_nv; 1. DR CDD; cd21171; NTD_alpha_betaCoV_Nsp15-like; 1. DR CDD; cd21689; stalk_CoV_Nsp13-like; 1. DR CDD; cd21401; ZBD_cv_Nsp13-like; 1. DR Gene3D; 3.40.50.11580; -; 1. DR Gene3D; 3.30.160.820; Nsp15 N-terminal domain-like; 1. DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2. DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1. DR InterPro; IPR027351; (+)RNA_virus_helicase_core_dom. DR InterPro; IPR046440; AV_NSP11N_COV_NSP15M. DR InterPro; IPR050534; Coronavir_polyprotein_1ab. DR InterPro; IPR043608; CoV_NSP15_M. DR InterPro; IPR043606; CoV_NSP15_N. DR InterPro; IPR043502; DNA/RNA_pol_sf. DR InterPro; IPR041679; DNA2/NAM7-like_C. DR InterPro; IPR037227; EndoU-like. DR InterPro; IPR046435; N7_MTase_CoV. DR InterPro; IPR043609; NendoU_nidovirus. DR InterPro; IPR044863; NIRAN. DR InterPro; IPR046438; NIV_2_O_MTASE. DR InterPro; IPR046436; NIV_EXON. DR InterPro; IPR047570; NSP12_IF_CoV. DR InterPro; IPR044343; NSP13_1B_dom_CoV. DR InterPro; IPR048673; NSP13_stalk_CoV. DR InterPro; IPR048672; NSP13_ZBD_CoV. DR InterPro; IPR027352; NSP13_ZBD_CoV-like. DR InterPro; IPR044315; NSP14_betaCoV. DR InterPro; IPR009466; NSP14_CoV. DR InterPro; IPR044330; NSP15_alpha_betaCoV_N. DR InterPro; IPR044322; NSP15_M_alpha_beta_CoV. DR InterPro; IPR043174; NSP15_middle_sf. DR InterPro; IPR042515; NSP15_N_CoV. DR InterPro; IPR009461; NSP16_CoV-like. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR046441; RdRp_CoV. DR InterPro; IPR009469; RdRp_N_CoV. DR InterPro; IPR001205; RNA-dir_pol_C. DR InterPro; IPR007094; RNA-dir_pol_PSvirus. DR InterPro; IPR029063; SAM-dependent_MTases_sf. DR PANTHER; PTHR43788; DNA2/NAM7 HELICASE FAMILY MEMBER; 1. DR PANTHER; PTHR43788:SF8; HELICASE WITH ZINC FINGER 2; 1. DR Pfam; PF13087; AAA_12; 1. DR Pfam; PF13604; AAA_30; 1. DR Pfam; PF06471; CoV_ExoN; 1. DR Pfam; PF06460; CoV_Methyltr_2; 1. DR Pfam; PF20631; CoV_NSP13_1B; 1. DR Pfam; PF20633; CoV_NSP13_stalk; 1. DR Pfam; PF20632; CoV_NSP13_ZBD; 1. DR Pfam; PF19215; CoV_NSP15_C; 1. DR Pfam; PF19216; CoV_NSP15_M; 1. DR Pfam; PF19219; CoV_NSP15_N; 1. DR Pfam; PF06478; CoV_RPol_N; 1. DR Pfam; PF00680; RdRP_1; 1. DR SUPFAM; SSF56672; DNA/RNA polymerases; 1. DR SUPFAM; SSF142877; EndoU-like; 1. DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1. DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1. DR PROSITE; PS51961; AV_NSP11N_COV_NSP15M; 1. DR PROSITE; PS51954; COV_N7_MTASE; 1. DR PROSITE; PS52000; COV_NSP12_IF; 1. DR PROSITE; PS51948; COV_NSP12_RDRP; 1. DR PROSITE; PS51960; COV_NSP15_NTD; 1. DR PROSITE; PS51653; CV_ZBD; 1. DR PROSITE; PS51958; NENDOU; 1. DR PROSITE; PS51947; NIRAN; 1. DR PROSITE; PS51955; NIV_2_O_MTASE; 1. DR PROSITE; PS51953; NIV_EXON; 1. DR PROSITE; PS51657; PSRV_HELICASE; 1. DR PROSITE; PS50507; RDRP_SSRNA_POS; 1. PE 3: Inferred from homology; KW ATP-binding {ECO:0000256|ARBA:ARBA00022840}; KW Endonuclease {ECO:0000256|ARBA:ARBA00022759, ECO:0000256|PROSITE- KW ProRule:PRU01303}; Exonuclease {ECO:0000256|PROSITE-ProRule:PRU01298}; KW Host cytoplasm {ECO:0000256|ARBA:ARBA00023200}; KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE- KW ProRule:PRU01303}; Metal-binding {ECO:0000256|ARBA:ARBA00022723}; KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE- KW ProRule:PRU01299}; KW Nuclease {ECO:0000256|ARBA:ARBA00022722, ECO:0000256|PROSITE- KW ProRule:PRU01298}; Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741}; KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695}; KW Ribosomal frameshifting {ECO:0000256|ARBA:ARBA00022758}; KW RNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022484}; KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE- KW ProRule:PRU01299}; Viral RNA replication {ECO:0000256|ARBA:ARBA00022953}; KW Zinc {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-ProRule:PRU00986}; KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE- KW ProRule:PRU00986}. FT DOMAIN 1..246 FT /note="NiRAN" FT /evidence="ECO:0000259|PROSITE:PS51947" FT DOMAIN 251..349 FT /note="Nsp12 Interface" FT /evidence="ECO:0000259|PROSITE:PS52000" FT DOMAIN 350..917 FT /note="Nsp12 RNA-dependent RNA polymerase" FT /evidence="ECO:0000259|PROSITE:PS51948" FT DOMAIN 597..759 FT /note="RdRp catalytic" FT /evidence="ECO:0000259|PROSITE:PS50507" FT DOMAIN 918..1001 FT /note="CV ZBD" FT /evidence="ECO:0000259|PROSITE:PS51653" FT DOMAIN 1174..1530 FT /note="(+)RNA virus helicase C-terminal" FT /evidence="ECO:0000259|PROSITE:PS51657" FT DOMAIN 1587..1802 FT /note="ExoN" FT /evidence="ECO:0000259|PROSITE:PS51953" FT DOMAIN 1811..2039 FT /note="N7-MTase" FT /evidence="ECO:0000259|PROSITE:PS51954" FT DOMAIN 2040..2100 FT /note="Nsp15 N-terminal oligomerization" FT /evidence="ECO:0000259|PROSITE:PS51960" FT DOMAIN 2101..2223 FT /note="AV-Nsp11N/CoV-Nsp15M" FT /evidence="ECO:0000259|PROSITE:PS51961" FT DOMAIN 2240..2379 FT /note="NendoU" FT /evidence="ECO:0000259|PROSITE:PS51958" FT DOMAIN 2384..2678 FT /note="Nidovirus-type SAM-dependent 2'-O-MTase" FT /evidence="ECO:0000259|PROSITE:PS51955" FT REGION 1925..1939 FT /note="GpppA-binding" FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01299" FT ACT_SITE 1605 FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01298" FT ACT_SITE 1607 FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01298" FT ACT_SITE 1706 FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01298" FT ACT_SITE 1783 FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01298" FT ACT_SITE 1788 FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01298" FT ACT_SITE 2270 FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01303" FT ACT_SITE 2285 FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01303" FT ACT_SITE 2325 FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01303" FT BINDING 1846..1852 FT /ligand="S-adenosyl-L-methionine" FT /ligand_id="ChEBI:CHEBI:59789" FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01299" FT NON_TER 1 FT /evidence="ECO:0000313|EMBL:AHC74097.1" SQ SEQUENCE 2685 AA; 303219 MW; 7638CB81574474A1 CRC64; ARIEPCSSGL STDVVFRAFD ICNYKAKVAG IGKYYKTNTC RFVELDDQGH HLDSYFVVKR HTMENYELEK HCYDLLRDCD AVAPHDFFIF DVDKVKTPHI VRQRLTEYTM MDLVYALRHF DQNSEVLKAI LVKYGCCDVT YFENKLWFDF VENPSVIGVY HKLGERVRQA ILNTVKFCDH MVKAGLVGVL TLDNQDLNGK WYDFGDFVIT QPGSGVAIVD SYYSYLMPVL SMTDCLAAET HRDCDFNKPL IEWPLTEYDF TDYKVQLFEK YFKYWDQTYH ANCVNCTDDR CVLHCANFNV LFAMTMPKTC FGPIVRKIFV DGVPFVVSCG YHYKELGLVM NMDVSLHRHR LSLKELMMYA ADPAMHIASS NAFLDLRTSC FSVAALTTGL TFQTVRPGNF NQDFYDFVVS KGFFKEGSSV TLKHFFFAQD GNAAITDYNY YSYNLPTMCD IKQMLFCMEV VNKYFEIYDG GCLNASEVVV NNLDKSAGHP FNKFGKARVY YESMSYQEQD ELFAMTKRNV IPTMTQMNLK YAISAKNRAR TVAGVSILST MTNRQYHQKM LKSMAATRGA TCVIGTTKFY GGWDFMLKTL YKDVDNPHLM GWDYPKCDRA MPNMCRIFAS LILARKHGTC CTTRDRFYRL ANECAQVLSE YVLCGGGYYV KPGGTSSGDA TTAYANSVFN ILQATTANVS ALMGANGNKI VDKEVKDMQF DLYVNVYRST SPDPKFVDKY YAFLNKHFSM MILSDDGVVC YNSDYAAKGY IAGIQNFKET LYYQNNVFMS EAKCWVETDL KKGPHEFCSQ HTLYIKDGDD GYFLPYPDPS RILSAGCFVD DIVKTDGTLM VERFVSLAID AYPLTKHEDI EYQNVFWVYL QYIEKLYKDL TGHMLDSYSV MLCGDNSAKF WEEAFYRDLY SSPTTLQAVG SCVVCHSQTS LRCGTCIRRP FLCCKCCYDH VIATPHKMVL SVSPYVCNAP GCGVSDVTKL YLGGMSYFCV DHRPVCSFPL CANGLVFGLY KNMCTGSPSI VEFNRLATCD WTESGDYTLA NTTTEPLKLF AAETLRATEE ASKQSYAIAT IKEIVGERQL LLVWEAGKSK PPLNRNYVFT GYHITKNSKV QLGEYIFERI DYSDAVSYKS STTYKLTVGD IFVLTSHSVA TLTAPTIVNQ ERYVKITGLY PTITVPEEFA SHVANFQKSG YSKYVTVQGP PGTGKSHFAI GLAIYYPTAR VVYTACSHAA VDALCEKAFK YLNIAKCSRI IPAKARVECY DRFKVNETNS QYLFSTINAL PETSADILVV DEVSMCTNYD LSIINARIKA KHIVYVGDPA QLPAPRTLLT RGTLEPENFN SVTRLMCNLG PDIFLSMCYR CPKEIVSTVS ALVYNNKLLA KKELSGQCFK ILYKGNVTHD ASSAINRPQL TFVKNFITAN PAWSKAVFIS PYNSQNAVAR SMLGLTTQTV DSSQGSEYQY VIFCQTADTA HANNINRFNV AITRAQKGIL CVMTSQALFE SLEFTELSFT NYKLQSQIVT GLFKDCSRET SGLSPAYAPT YVSVDDKYKT SDELCVNLNL PANVPYSRVI SRMGFKLDAT VPGYPKLFIT REEAVRQVRS WIGFDVEGAH ASRNACGTNV PLQLGFSTGV NFVVQPVGVV DTEWGNMLTG IAARPPPGEQ FKHLVPLMHK GAAWPIVRRR IVQMLSDTLD KLSDYCTFVC WAHGFELTSA SYFCKIGKEQ KCCMCNRRAA AYSSPLQSYA CWTHSCGYDY VYNPFFVDVQ QWGYVGNLAT NHDRYCSVHQ GAHVASNDAI MTRCLAIHSC FIERVDWDIE YPYISHEKKL NSCCRIVERN VVRAALLAGS FDKVYDIGNP KGIPIVDDPV VDWHYFDAQP LTRKVQQLFY TEDMASRFAD GLCLFWNCNV PKYPNNAIVC RFDTRVHSEF NLPGCDGGSL YVNKHAFHTP AYDVSAFRDL KPLPFFYYST TPCEVHGNGS MIEDIDYVPL KSAVCITTCN LGGAVCRKHA TEYREYMEAY NLVSASGFRL WCYKTFDIYN LWSTFTKVQG LENIAFNVVK QGHFIGVEGE LPVAVVNDKI FTKSGVNDIC MFENKTTLPT NIAFELYAKR AVRSHPDFKL LHNLQADICY KFVLWDYERS NIYGTATIGV CKYTDIDVNS ALNICFDIRD NGSLEKFMST PNAIFISDRK IKKYPCMVGP DYAYFNGAII RDSDVVKQPV KFYLYKKVNN EFIDPTECIY TQSRSCSDFL PLSDMEKDFL SFDSDVFIKK YGLENYAFEH VVYGDFSHTT LGGLHLLIGL YKKQQEGHII MEEMLKGSST IHNYFITETN TAAFKAVCSV IDLKLDDFVM ILKSQDLGVV SKVVKVPIDL TMIEFMLWCK DGQVQTFYPR LQASADWKPG HAMPSLFKVQ NVNLERCELA NYKQSIPMPR GVHMNIAKYM QLCQYLNTCT LAVPANMRVI HFGAGSDKGI APGTSVLRQW LPIDAIIIDN DLNEFVSDAD ITLFGDCVTV RVGQQVDLVI SDMYDPTTKN VTGSNESKAL FFTYLCNLIN NNLALGGSVA IKITEHSWSV ELYELMGKFA WWTVFCTNAN ASSSEGFLLG INYLGTIKEN IDGGAMHANY IFWRNSTPMN LSTYSLFDLS KFQLKLKGTP VLQLKESQIN ELVISLLSQG KLLIRDNDTL SVSTDVLVNT YRKLR //