ID UL128_HCMVM Reviewed; 171 AA. AC V9LLX6; DT 10-OCT-2018, integrated into UniProtKB/Swiss-Prot. DT 10-MAY-2017, sequence version 1. DT 07-NOV-2018, entry version 3. DE RecName: Full=Envelope protein UL128; GN Name=UL128; OS Human cytomegalovirus (strain Merlin) (HHV-5) (Human herpesvirus 5). OC Viruses; dsDNA viruses, no RNA stage; Herpesvirales; Herpesviridae; OC Betaherpesvirinae; Cytomegalovirus. OX NCBI_TaxID=295027; OH NCBI_TaxID=9606; Homo sapiens (Human). RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RA Davison A.J.; RT "Human cytomegalovirus RL11 gene family: variation, recombination and RT transcription."; RL Submitted (AUG-2012) to the EMBL/GenBank/DDBJ databases. RN [2] RP FUNCTION, AND INTERACTION WITH GH; GL; UL130 AND UL131A. RX PubMed=17942555; DOI=10.1128/JVI.01910-07; RA Ryckman B.J., Rainish B.L., Chase M.C., Borton J.A., Nelson J.A., RA Jarvis M.A., Johnson D.C.; RT "Characterization of the human cytomegalovirus gH/gL/UL128-131 complex RT that mediates entry into epithelial and endothelial cells."; RL J. Virol. 82:60-70(2008). RN [3] RP FUNCTION. RX PubMed=21367908; DOI=10.1128/JVI.02100-10; RA Straschewski S., Patrone M., Walther P., Gallina A., Mertens T., RA Frascaroli G.; RT "Protein pUL128 of human cytomegalovirus is necessary for monocyte RT infection and blocking of migration."; RL J. Virol. 85:5150-5158(2011). RN [4] RP FUNCTION. RX PubMed=23853586; DOI=10.1371/journal.ppat.1003463; RA Nogalski M.T., Chan G.C., Stevenson E.V., Collins-McMillen D.K., RA Yurochko A.D.; RT "The HCMV gH/gL/UL128-131 complex triggers the specific cellular RT activation required for efficient viral internalization into target RT monocytes."; RL PLoS Pathog. 9:E1003463-E1003463(2013). RN [5] RP SUBCELLULAR LOCATION. RX PubMed=23804643; DOI=10.1128/JVI.01167-13; RA Zhou M., Yu Q., Wechsler A., Ryckman B.J.; RT "Comparative analysis of gO isoforms reveals that strains of human RT cytomegalovirus differ in the ratio of gH/gL/gO and gH/gL/UL128-131 in RT the virion envelope."; RL J. Virol. 87:9680-9690(2013). RN [6] RP REVIEW. RX PubMed=24957713; RA Tao R., Xu J., Gao H., Zhao W., Shang S.; RT "Characteristics and functions of human cytomegalovirus UL128 RT gene/protein."; RL Acta Virol. 58:103-107(2014). RN [7] RP FUNCTION, AND INTERACTION WITH HOST NRP2. RX PubMed=30057110; DOI=10.1016/j.cell.2018.06.028; RA Martinez-Martin N., Marcandalli J., Huang C.S., Arthur C.P., RA Perotti M., Foglierini M., Ho H., Dosey A.M., Shriver S., Payandeh J., RA Leitner A., Lanzavecchia A., Perez L., Ciferri C.; RT "An Unbiased Screen for Human Cytomegalovirus Identifies Neuropilin-2 RT as a Central Viral Receptor."; RL Cell 0:0-0(2018). CC -!- FUNCTION: Plays a role in viral entry into host cells. Forms a CC pentameric complex at the surface of the viral envelope together CC with gH, gL, UL130 and UL131. This complex is required for entry CC in epithelial, endothelial and myeloid host cells CC (PubMed:23853586, PubMed:17942555). Mechanistically, engages host CC receptor(s) including neurophilin 2/NRP2 to mediate infection CC (PubMed:30057110). Additionally, monomeric UL128 may interfere CC with certain inflammatory cytokines to increase infection and CC dissemination by blocking monocytes migration (PubMed:21367908). CC {ECO:0000269|PubMed:17942555, ECO:0000269|PubMed:21367908, CC ECO:0000269|PubMed:23853586, ECO:0000269|PubMed:30057110}. CC -!- SUBUNIT: Forms the envelope pentamer complex (PC) composed of gH, CC gL, UL128, UL130, and UL131A (PubMed:17942555). The pentamer CC interacts with host NRP2 (PubMed:30057110). CC {ECO:0000269|PubMed:17942555, ECO:0000269|PubMed:30057110}. CC -!- SUBCELLULAR LOCATION: Virion membrane CC {ECO:0000269|PubMed:23804643}. Note=Found as a pentameric complex CC at the surface of virion envelope. {ECO:0000269|PubMed:23804643}. CC -!- SIMILARITY: Belongs to the HHV-5 UL128 protein family. CC {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; JX512197; AFR54607.1; -; Genomic_DNA. DR Proteomes; UP000169440; Genome. DR GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW. DR GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell. DR GO; GO:0098670; P:entry receptor-mediated virion attachment to host cell; IEA:UniProtKB-KW. DR GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW. PE 1: Evidence at protein level; KW Complete proteome; Host-virus interaction; Membrane; KW Viral attachment to host cell; KW Viral attachment to host entry receptor; Viral envelope protein; KW Virion; Virus entry into host cell. FT CHAIN 1 171 Envelope protein UL128. FT /FTId=PRO_0000445468. SQ SEQUENCE 171 AA; 19773 MW; 9973D2EF8BF282ED CRC64; MSPKNLTPFL TALWLLLDHS RVPRVRAEEC CEFINVNHPP ERCYDFKMCN RFTVALRCPD GEVCYSPEKT AEIRGIVTTM THSLTRQVVH NKLTSCNYNP LYLEADGRIR CGKVNDKAQY LLGAAGSVPY RWINLEYDKI TRIVGLNQYL ESVKKHKRLD VCRAKMGYML Q //