ID   V9GXP1_MOUSE            Unreviewed;       177 AA.
AC   V9GXP1;
DT   19-FEB-2014, integrated into UniProtKB/TrEMBL.
DT   19-FEB-2014, sequence version 1.
DT   13-SEP-2023, entry version 62.
DE   RecName: Full=diphthine methyl ester synthase {ECO:0000256|ARBA:ARBA00011927};
DE            EC=2.1.1.314 {ECO:0000256|ARBA:ARBA00011927};
GN   Name=Dph5 {ECO:0000313|Ensembl:ENSMUSP00000139249.2,
GN   ECO:0000313|MGI:MGI:1916990};
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090 {ECO:0000313|Ensembl:ENSMUSP00000139249.2, ECO:0000313|Proteomes:UP000000589};
RN   [1] {ECO:0000313|Ensembl:ENSMUSP00000139249.2, ECO:0000313|Proteomes:UP000000589}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J {ECO:0000313|Ensembl:ENSMUSP00000139249.2,
RC   ECO:0000313|Proteomes:UP000000589};
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [2] {ECO:0007829|PubMed:21183079}
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
RN   [3] {ECO:0000313|Ensembl:ENSMUSP00000139249.2}
RP   IDENTIFICATION.
RC   STRAIN=C57BL/6J {ECO:0000313|Ensembl:ENSMUSP00000139249.2};
RG   Ensembl;
RL   Submitted (MAY-2023) to UniProtKB.
CC   -!- FUNCTION: S-adenosyl-L-methionine-dependent methyltransferase that
CC       catalyzes four methylations of the modified target histidine residue in
CC       translation elongation factor 2 (EF-2), to form an intermediate called
CC       diphthine methyl ester. The four successive methylation reactions
CC       represent the second step of diphthamide biosynthesis.
CC       {ECO:0000256|ARBA:ARBA00004006}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation
CC         elongation factor 2] + 4 S-adenosyl-L-methionine = diphthine methyl
CC         ester-[translation elongation factor 2] + 3 H(+) + 4 S-adenosyl-L-
CC         homocysteine; Xref=Rhea:RHEA:42652, Rhea:RHEA-COMP:9749, Rhea:RHEA-
CC         COMP:10173, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:73995, ChEBI:CHEBI:79005; EC=2.1.1.314;
CC         Evidence={ECO:0000256|ARBA:ARBA00000054};
CC   -!- PATHWAY: Protein modification; peptidyl-diphthamide biosynthesis.
CC       {ECO:0000256|ARBA:ARBA00005156}.
CC   -!- SIMILARITY: Belongs to the diphthine synthase family.
CC       {ECO:0000256|ARBA:ARBA00006729}.
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DR   RefSeq; XP_006502102.1; XM_006502039.2.
DR   AlphaFoldDB; V9GXP1; -.
DR   SMR; V9GXP1; -.
DR   MaxQB; V9GXP1; -.
DR   PeptideAtlas; V9GXP1; -.
DR   ProteomicsDB; 336434; -.
DR   Antibodypedia; 33698; 35 antibodies from 9 providers.
DR   Ensembl; ENSMUST00000185098.8; ENSMUSP00000139249.2; ENSMUSG00000033554.18.
DR   AGR; MGI:1916990; -.
DR   MGI; MGI:1916990; Dph5.
DR   VEuPathDB; HostDB:ENSMUSG00000033554; -.
DR   GeneTree; ENSGT00390000010568; -.
DR   HOGENOM; CLU_066040_2_0_1; -.
DR   OMA; TAGDPMV; -.
DR   UniPathway; UPA00559; -.
DR   BioGRID-ORCS; 69740; 29 hits in 79 CRISPR screens.
DR   ChiTaRS; Dph5; mouse.
DR   Proteomes; UP000000589; Chromosome 3.
DR   Bgee; ENSMUSG00000033554; Expressed in paneth cell and 231 other tissues.
DR   ExpressionAtlas; V9GXP1; baseline and differential.
DR   GO; GO:0004164; F:diphthine synthase activity; IEA:InterPro.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   GO; GO:0017183; P:peptidyl-diphthamide biosynthetic process from peptidyl-histidine; IEA:UniProtKB-UniPathway.
DR   CDD; cd11647; DHP5_DphB; 1.
DR   InterPro; IPR000878; 4pyrrol_Mease.
DR   InterPro; IPR035996; 4pyrrol_Methylase_sf.
DR   InterPro; IPR014777; 4pyrrole_Mease_sub1.
DR   InterPro; IPR014776; 4pyrrole_Mease_sub2.
DR   InterPro; IPR004551; Dphthn_synthase.
DR   NCBIfam; TIGR00522; dph5; 1.
DR   PANTHER; PTHR10882:SF0; DIPHTHINE METHYL ESTER SYNTHASE; 1.
DR   PANTHER; PTHR10882; DIPHTHINE SYNTHASE; 1.
DR   Pfam; PF00590; TP_methylase; 1.
DR   SUPFAM; SSF53790; Tetrapyrrole methylase; 1.
PE   1: Evidence at protein level;
KW   Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW   Proteomics identification {ECO:0007829|EPD:V9GXP1,
KW   ECO:0007829|MaxQB:V9GXP1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000589};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT   DOMAIN          1..164
FT                   /note="Tetrapyrrole methylase"
FT                   /evidence="ECO:0000259|Pfam:PF00590"
SQ   SEQUENCE   177 AA;  19921 MW;  B3FE40D4B6C13472 CRC64;
     MLYLIGLGLG DAKDITVKGL EVVRRCSRVY LEAYTSVLTV GKEALEEFYG RKLILADREE
     VEQEADNIFK DADVSDVAFL VVGDPFGATT HSDLILRATK LGIPYQVIHN ASIMNAVGCC
     GLQLYRFGET VSIVFWTDTW RPESFFDKVK RNRANGMHTL CLLDIKVKEQ SLENLIR
//