ID V9GWU6_MOUSE Unreviewed; 99 AA. AC V9GWU6; DT 19-FEB-2014, integrated into UniProtKB/TrEMBL. DT 19-FEB-2014, sequence version 1. DT 02-OCT-2024, entry version 59. DE RecName: Full=Transmembrane ascorbate-dependent reductase CYB561 {ECO:0000256|ARBA:ARBA00024231}; DE AltName: Full=Cytochrome b-561 {ECO:0000256|ARBA:ARBA00030896}; DE AltName: Full=Cytochrome b561 {ECO:0000256|ARBA:ARBA00032709}; DE Flags: Fragment; GN Name=Cyb561 {ECO:0000313|Ensembl:ENSMUSP00000138838.2, GN ECO:0000313|MGI:MGI:103253}; OS Mus musculus (Mouse). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; OC Murinae; Mus; Mus. OX NCBI_TaxID=10090 {ECO:0000313|Ensembl:ENSMUSP00000138838.2, ECO:0000313|Proteomes:UP000000589}; RN [1] {ECO:0000313|Ensembl:ENSMUSP00000138838.2, ECO:0000313|Proteomes:UP000000589} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=C57BL/6J {ECO:0000313|Ensembl:ENSMUSP00000138838.2, RC ECO:0000313|Proteomes:UP000000589}; RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112; RG Mouse Genome Sequencing Consortium; RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S., RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., RA Eichler E.E., Ponting C.P.; RT "Lineage-specific biology revealed by a finished genome assembly of the RT mouse."; RL PLoS Biol. 7:E1000112-E1000112(2009). RN [2] {ECO:0000313|Ensembl:ENSMUSP00000138838.2} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=C57BL/6J {ECO:0000313|Ensembl:ENSMUSP00000138838.2}; RX PubMed=21750661; RA Church D.M., Schneider V.A., Graves T., Auger K., Cunningham F., Bouk N., RA Chen H.C., Agarwala R., McLaren W.M., Ritchie G.R., Albracht D., RA Kremitzki M., Rock S., Kotkiewicz H., Kremitzki C., Wollam A., Trani L., RA Fulton L., Fulton R., Matthews L., Whitehead S., Chow W., Torrance J., RA Dunn M., Harden G., Threadgold G., Wood J., Collins J., Heath P., RA Griffiths G., Pelan S., Grafham D., Eichler E.E., Weinstock G., RA Mardis E.R., Wilson R.K., Howe K., Flicek P., Hubbard T.; RT "Modernizing reference genome assemblies."; RL PLoS Biol. 9:e1001091-e1001091(2011). RN [3] {ECO:0000313|Ensembl:ENSMUSP00000138838.2} RP IDENTIFICATION. RC STRAIN=C57BL/6J {ECO:0000313|Ensembl:ENSMUSP00000138838.2}; RG Ensembl; RL Submitted (JUN-2024) to UniProtKB. CC -!- CATALYTIC ACTIVITY: CC Reaction=L-ascorbate(in) + monodehydro-L-ascorbate radical(out) = L- CC ascorbate(out) + monodehydro-L-ascorbate radical(in); CC Xref=Rhea:RHEA:66524, ChEBI:CHEBI:38290, ChEBI:CHEBI:59513; CC Evidence={ECO:0000256|ARBA:ARBA00024163}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:66525; CC Evidence={ECO:0000256|ARBA:ARBA00024163}; CC -!- COFACTOR: CC Name=heme b; Xref=ChEBI:CHEBI:60344; CC Evidence={ECO:0000256|ARBA:ARBA00001970}; CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, CC chromaffin granule membrane {ECO:0000256|ARBA:ARBA00024185}; Multi-pass CC membrane protein {ECO:0000256|ARBA:ARBA00024185}. Membrane CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein CC {ECO:0000256|ARBA:ARBA00004141}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR AlphaFoldDB; V9GWU6; -. DR SMR; V9GWU6; -. DR Antibodypedia; 18653; 105 antibodies from 17 providers. DR Ensembl; ENSMUST00000150563.3; ENSMUSP00000138838.2; ENSMUSG00000019590.17. DR AGR; MGI:103253; -. DR MGI; MGI:103253; Cyb561. DR VEuPathDB; HostDB:ENSMUSG00000019590; -. DR GeneTree; ENSGT00950000183197; -. DR OMA; EFHYHPT; -. DR ChiTaRS; Cyb561; mouse. DR Proteomes; UP000000589; Chromosome 11. DR Bgee; ENSMUSG00000019590; Expressed in choroid plexus of fourth ventricle and 241 other cell types or tissues. DR ExpressionAtlas; V9GWU6; baseline and differential. DR GO; GO:0042584; C:chromaffin granule membrane; IEA:UniProtKB-SubCell. DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. DR GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro. DR Gene3D; 1.20.120.1770; -; 1. DR InterPro; IPR043205; CYB561/CYBRD1-like. DR InterPro; IPR006593; Cyt_b561/ferric_Rdtase_TM. DR PANTHER; PTHR10106; CYTOCHROME B561-RELATED; 1. DR PANTHER; PTHR10106:SF14; TRANSMEMBRANE ASCORBATE-DEPENDENT REDUCTASE CYB561; 1. DR Pfam; PF03188; Cytochrom_B561; 1. DR PROSITE; PS50939; CYTOCHROME_B561; 1. PE 4: Predicted; KW Cytoplasmic vesicle {ECO:0000256|ARBA:ARBA00023329}; KW Electron transport {ECO:0000256|ARBA:ARBA00022982}; KW Heme {ECO:0000256|ARBA:ARBA00022617}; Iron {ECO:0000256|ARBA:ARBA00022617}; KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius}; KW Metal-binding {ECO:0000256|ARBA:ARBA00022723}; KW Reference proteome {ECO:0000313|Proteomes:UP000000589}; KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius}; KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989, KW ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00022448}. FT TRANSMEM 12..36 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 56..74 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 81..98 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT DOMAIN 18..99 FT /note="Cytochrome b561" FT /evidence="ECO:0000259|PROSITE:PS50939" FT NON_TER 99 FT /evidence="ECO:0000313|Ensembl:ENSMUSP00000138838.2" SQ SEQUENCE 99 AA; 10933 MW; 656FA0B3B6BDA902 CRC64; MEHSSASVPA ALPYYVAFSQ LLGLTVVAVT GAWLGLYRGG IAWESSLQFN VHPLCMVIGM IFLQGDALLV YRVFRREAKR TTKILHGLLH VFAFIIALV //