ID V8QLJ9_9BURK Unreviewed; 337 AA. AC V8QLJ9; DT 19-FEB-2014, integrated into UniProtKB/TrEMBL. DT 19-FEB-2014, sequence version 1. DT 16-MAR-2016, entry version 12. DE RecName: Full=tRNA-dihydrouridine synthase B {ECO:0000256|HAMAP-Rule:MF_02042}; DE EC=1.3.1.- {ECO:0000256|HAMAP-Rule:MF_02042, ECO:0000256|PIRNR:PIRNR006621}; GN Name=dusB {ECO:0000256|HAMAP-Rule:MF_02042}; GN ORFNames=W822_21015 {ECO:0000313|EMBL:ETF00846.1}; OS Advenella kashmirensis W13003. OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; OC Alcaligenaceae. OX NCBI_TaxID=1424334 {ECO:0000313|EMBL:ETF00846.1, ECO:0000313|Proteomes:UP000018733}; RN [1] {ECO:0000313|EMBL:ETF00846.1, ECO:0000313|Proteomes:UP000018733} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=W13003 {ECO:0000313|EMBL:ETF00846.1, RC ECO:0000313|Proteomes:UP000018733}; RX PubMed=24482505; RA Wang X., Jin D., Zhou L., Wu L., An W., Zhao L.; RT "Draft Genome Sequence of Advenella kashmirensis Strain W13003, a RT Polycyclic Aromatic Hydrocarbon-Degrading Bacterium."; RL Genome Announc. 2:e00003-14(2014). CC -!- FUNCTION: Catalyzes the synthesis of 5,6-dihydrouridine (D), a CC modified base found in the D-loop of most tRNAs, via the reduction CC of the C5-C6 double bond in target uridines. {ECO:0000256|HAMAP- CC Rule:MF_02042, ECO:0000256|PIRNR:PIRNR006621}. CC -!- CATALYTIC ACTIVITY: 5,6-dihydrouracil in tRNA + NAD(P)(+) = uracil CC in tRNA + NAD(P)H. {ECO:0000256|HAMAP-Rule:MF_02042}. CC -!- COFACTOR: CC Name=FMN; Xref=ChEBI:CHEBI:58210; Evidence={ECO:0000256|HAMAP- CC Rule:MF_02042, ECO:0000256|PIRNR:PIRNR006621}; CC -!- SIMILARITY: Belongs to the Dus family. DusB subfamily. CC {ECO:0000256|HAMAP-Rule:MF_02042}. CC -!- SIMILARITY: Belongs to the dus family. CC {ECO:0000256|PIRNR:PIRNR006621}. CC -!- CAUTION: The sequence shown here is derived from an CC EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is CC preliminary data. {ECO:0000313|EMBL:ETF00846.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AYXT01000013; ETF00846.1; -; Genomic_DNA. DR RefSeq; WP_024007124.1; NZ_KI650982.1. DR EnsemblBacteria; ETF00846; ETF00846; W822_21015. DR Proteomes; UP000018733; Unassembled WGS sequence. DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro. DR GO; GO:0017150; F:tRNA dihydrouridine synthase activity; IEA:InterPro. DR Gene3D; 1.10.1200.80; -; 1. DR Gene3D; 3.20.20.70; -; 1. DR HAMAP; MF_02042; DusB_subfam; 1. DR InterPro; IPR013785; Aldolase_TIM. DR InterPro; IPR032887; DusB. DR InterPro; IPR024036; tRNA-dHydroUridine_Synthase_C. DR InterPro; IPR004652; tRNA_dU_NifR3. DR InterPro; IPR001269; tRNA_hU_synthase. DR InterPro; IPR018517; tRNA_hU_synthase_CS. DR PANTHER; PTHR11082; PTHR11082; 1. DR Pfam; PF01207; Dus; 1. DR PIRSF; PIRSF006621; Dus; 1. DR TIGRFAMs; TIGR00737; nifR3_yhdG; 1. DR PROSITE; PS01136; UPF0034; 1. PE 3: Inferred from homology; KW Complete proteome {ECO:0000313|Proteomes:UP000018733}; KW Flavoprotein {ECO:0000256|HAMAP-Rule:MF_02042, KW ECO:0000256|PIRNR:PIRNR006621}; KW FMN {ECO:0000256|HAMAP-Rule:MF_02042, ECO:0000256|PIRNR:PIRNR006621}; KW NADP {ECO:0000256|HAMAP-Rule:MF_02042}; KW Oxidoreductase {ECO:0000256|HAMAP-Rule:MF_02042, KW ECO:0000256|PIRNR:PIRNR006621}; KW RNA-binding {ECO:0000256|HAMAP-Rule:MF_02042}; KW tRNA processing {ECO:0000256|HAMAP-Rule:MF_02042, KW ECO:0000256|PIRNR:PIRNR006621}; KW tRNA-binding {ECO:0000256|HAMAP-Rule:MF_02042}. FT NP_BIND 16 18 FMN. {ECO:0000256|HAMAP-Rule:MF_02042}. FT NP_BIND 203 205 FMN. {ECO:0000256|HAMAP-Rule:MF_02042}. FT NP_BIND 227 228 FMN. {ECO:0000256|HAMAP-Rule:MF_02042}. FT ACT_SITE 100 100 Proton donor. {ECO:0000256|HAMAP-Rule: FT MF_02042, ECO:0000256|PIRSR:PIRSR006621- FT 1}. FT BINDING 70 70 FMN. {ECO:0000256|HAMAP-Rule:MF_02042}. FT BINDING 142 142 FMN. {ECO:0000256|HAMAP-Rule:MF_02042}. SQ SEQUENCE 337 AA; 37128 MW; 693299B77C5A580F CRC64; MQIGAWTLPN PVFVAPMAGV TDRPFRQLCK QLGAGYAVSE MAASNPALWD SVKTSRRLNH DGEIDPIAVQ IAGADPVMLA EAAVFNIRKG ARIIDINMGC PVKKVCHVAS GSALLRDEEL VARILQTVVD ACRPFDVPVT LKTRTGWDRQ SRNALRIARL AEDAGIAALT LHGRTRCDLY AGQAEYDTIA AVKAAISIPV IANGDITDPL KAQYVLNYTG ADAIMIGRAA QGNPWIFREI NHYLETGTFL APPSWAHMRD VLLAHLDHHY QFYGEYTGVR TARKHIGWYI GNLPDAELFR QQMNCLETCQ AQYNAVNQWF TKLVNESAPR VPLAAVH //