ID V8QLJ9_9BURK Unreviewed; 337 AA. AC V8QLJ9; DT 19-FEB-2014, integrated into UniProtKB/TrEMBL. DT 19-FEB-2014, sequence version 1. DT 09-DEC-2015, entry version 10. DE RecName: Full=tRNA-dihydrouridine synthase {ECO:0000256|PIRNR:PIRNR006621}; DE EC=1.3.1.- {ECO:0000256|PIRNR:PIRNR006621}; GN ORFNames=W822_21015 {ECO:0000313|EMBL:ETF00846.1}; OS Advenella kashmirensis W13003. OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; OC Alcaligenaceae. OX NCBI_TaxID=1424334 {ECO:0000313|EMBL:ETF00846.1}; RN [1] {ECO:0000313|EMBL:ETF00846.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=W13003 {ECO:0000313|EMBL:ETF00846.1}; RX PubMed=24482505; RA Wang X., Jin D., Zhou L., Wu L., An W., Zhao L.; RT "Draft Genome Sequence of Advenella kashmirensis Strain W13003, a RT Polycyclic Aromatic Hydrocarbon-Degrading Bacterium."; RL Genome Announc. 2:e00003-14(2014). CC -!- FUNCTION: Catalyzes the synthesis of 5,6-dihydrouridine (D), a CC modified base found in the D-loop of most tRNAs, via the reduction CC of the C5-C6 double bond in target uridines. CC {ECO:0000256|PIRNR:PIRNR006621}. CC -!- COFACTOR: CC Name=FMN; Xref=ChEBI:CHEBI:58210; CC Evidence={ECO:0000256|PIRNR:PIRNR006621}; CC -!- SIMILARITY: Belongs to the dus family. CC {ECO:0000256|PIRNR:PIRNR006621}. CC -!- CAUTION: The sequence shown here is derived from an CC EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is CC preliminary data. {ECO:0000313|EMBL:ETF00846.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AYXT01000013; ETF00846.1; -; Genomic_DNA. DR RefSeq; WP_024007124.1; NZ_KI650982.1. DR EnsemblBacteria; ETF00846; ETF00846; W822_21015. DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro. DR GO; GO:0017150; F:tRNA dihydrouridine synthase activity; IEA:InterPro. DR Gene3D; 1.10.1200.80; -; 1. DR Gene3D; 3.20.20.70; -; 1. DR InterPro; IPR013785; Aldolase_TIM. DR InterPro; IPR024036; tRNA-dHydroUridine_Synthase_C. DR InterPro; IPR004652; tRNA_dU_NifR3. DR InterPro; IPR001269; tRNA_hU_synthase. DR InterPro; IPR018517; tRNA_hU_synthase_CS. DR PANTHER; PTHR11082; PTHR11082; 1. DR Pfam; PF01207; Dus; 1. DR PIRSF; PIRSF006621; Dus; 1. DR TIGRFAMs; TIGR00737; nifR3_yhdG; 1. DR PROSITE; PS01136; UPF0034; 1. PE 3: Inferred from homology; KW Flavoprotein {ECO:0000256|PIRNR:PIRNR006621}; KW FMN {ECO:0000256|PIRNR:PIRNR006621}; KW Oxidoreductase {ECO:0000256|PIRNR:PIRNR006621}; KW tRNA processing {ECO:0000256|PIRNR:PIRNR006621}. FT ACT_SITE 100 100 Proton donor. {ECO:0000256|PIRSR: FT PIRSR006621-1}. SQ SEQUENCE 337 AA; 37128 MW; 693299B77C5A580F CRC64; MQIGAWTLPN PVFVAPMAGV TDRPFRQLCK QLGAGYAVSE MAASNPALWD SVKTSRRLNH DGEIDPIAVQ IAGADPVMLA EAAVFNIRKG ARIIDINMGC PVKKVCHVAS GSALLRDEEL VARILQTVVD ACRPFDVPVT LKTRTGWDRQ SRNALRIARL AEDAGIAALT LHGRTRCDLY AGQAEYDTIA AVKAAISIPV IANGDITDPL KAQYVLNYTG ADAIMIGRAA QGNPWIFREI NHYLETGTFL APPSWAHMRD VLLAHLDHHY QFYGEYTGVR TARKHIGWYI GNLPDAELFR QQMNCLETCQ AQYNAVNQWF TKLVNESAPR VPLAAVH //