ID V4KDB3_EUTSA Unreviewed; 712 AA. AC V4KDB3; DT 22-JAN-2014, integrated into UniProtKB/TrEMBL. DT 22-JAN-2014, sequence version 1. DT 02-DEC-2020, entry version 23. DE RecName: Full=Amine oxidase {ECO:0000256|RuleBase:RU000672}; DE EC=1.4.3.- {ECO:0000256|RuleBase:RU000672}; GN ORFNames=EUTSA_v10023306mg {ECO:0000313|EMBL:ESQ29104.1}; OS Eutrema salsugineum (Saltwater cress) (Sisymbrium salsugineum). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; malvids; Brassicales; Brassicaceae; Eutremeae; Eutrema. OX NCBI_TaxID=72664 {ECO:0000313|EMBL:ESQ29104.1, ECO:0000313|Proteomes:UP000030689}; RN [1] {ECO:0000313|EMBL:ESQ29104.1, ECO:0000313|Proteomes:UP000030689} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RX PubMed=23518688; DOI=10.3389/fpls.2013.00046; RA Yang R., Jarvis D.E., Chen H., Beilstein M.A., Grimwood J., Jenkins J., RA Shu S., Prochnik S., Xin M., Ma C., Schmutz J., Wing R.A., RA Mitchell-Olds T., Schumaker K.S., Wang X.; RT "The Reference Genome of the Halophytic Plant Eutrema salsugineum."; RL Front. Plant Sci. 4:46-46(2013). CC -!- CATALYTIC ACTIVITY: CC Reaction=an aliphatic amine + H2O + O2 = an aldehyde + H2O2 + NH4(+); CC Xref=Rhea:RHEA:16153, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, CC ChEBI:CHEBI:16240, ChEBI:CHEBI:17478, ChEBI:CHEBI:28938, CC ChEBI:CHEBI:58001; EC=1.4.3.21; CC Evidence={ECO:0000256|ARBA:ARBA00001138}; CC -!- COFACTOR: CC Name=Cu cation; Xref=ChEBI:CHEBI:23378; CC Evidence={ECO:0000256|ARBA:ARBA00001935}; CC -!- COFACTOR: CC Name=L-topaquinone; Xref=ChEBI:CHEBI:79027; CC Evidence={ECO:0000256|PIRSR:PIRSR600269-51}; CC Note=Contains 1 topaquinone per subunit. CC {ECO:0000256|PIRSR:PIRSR600269-51}; CC -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}. CC -!- PTM: Topaquinone (TPQ) is generated by copper-dependent autoxidation of CC a specific tyrosyl residue. {ECO:0000256|PIRSR:PIRSR600269-51, CC ECO:0000256|RuleBase:RU000672}. CC -!- SIMILARITY: Belongs to the copper/topaquinone oxidase family. CC {ECO:0000256|ARBA:ARBA00007983, ECO:0000256|RuleBase:RU000672}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; KI517881; ESQ29104.1; -; Genomic_DNA. DR RefSeq; XP_006391818.1; XM_006391756.1. DR STRING; 72664.XP_006391818.1; -. DR GeneID; 18010301; -. DR KEGG; eus:EUTSA_v10023306mg; -. DR eggNOG; KOG1186; Eukaryota. DR OrthoDB; 1320015at2759; -. DR Proteomes; UP000030689; Unassembled WGS sequence. DR GO; GO:0052595; F:aliphatic-amine oxidase activity; IEA:UniProtKB-EC. DR GO; GO:0052594; F:aminoacetone:oxygen oxidoreductase(deaminating) activity; IEA:UniProtKB-EC. DR GO; GO:0005507; F:copper ion binding; IEA:InterPro. DR GO; GO:0052596; F:phenethylamine:oxygen oxidoreductase (deaminating) activity; IEA:UniProtKB-EC. DR GO; GO:0008131; F:primary amine oxidase activity; IEA:UniProtKB-EC. DR GO; GO:0048038; F:quinone binding; IEA:InterPro. DR GO; GO:0052593; F:tryptamine:oxygen oxidoreductase (deaminating) activity; IEA:UniProtKB-EC. DR GO; GO:0009308; P:amine metabolic process; IEA:UniProtKB-UniRule. DR Gene3D; 2.70.98.20; -; 1. DR InterPro; IPR000269; Cu_amine_oxidase. DR InterPro; IPR015798; Cu_amine_oxidase_C. DR InterPro; IPR036460; Cu_amine_oxidase_C_sf. DR InterPro; IPR016182; Cu_amine_oxidase_N-reg. DR InterPro; IPR015800; Cu_amine_oxidase_N2. DR InterPro; IPR015802; Cu_amine_oxidase_N3. DR PANTHER; PTHR10638; PTHR10638; 1. DR Pfam; PF01179; Cu_amine_oxid; 1. DR Pfam; PF02727; Cu_amine_oxidN2; 1. DR Pfam; PF02728; Cu_amine_oxidN3; 1. DR SUPFAM; SSF49998; SSF49998; 1. DR SUPFAM; SSF54416; SSF54416; 2. DR PROSITE; PS01165; COPPER_AMINE_OXID_2; 1. PE 3: Inferred from homology; KW Copper {ECO:0000256|ARBA:ARBA00023008, ECO:0000256|RuleBase:RU000672}; KW Metal-binding {ECO:0000256|ARBA:ARBA00022723, KW ECO:0000256|RuleBase:RU000672}; KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002, KW ECO:0000256|RuleBase:RU000672}; KW Reference proteome {ECO:0000313|Proteomes:UP000030689}; KW TPQ {ECO:0000256|ARBA:ARBA00022772, ECO:0000256|PIRSR:PIRSR600269-50, KW ECO:0000256|RuleBase:RU000672}. FT DOMAIN 60..142 FT /note="Cu_amine_oxidN2" FT /evidence="ECO:0000259|Pfam:PF02727" FT DOMAIN 152..250 FT /note="Cu_amine_oxidN3" FT /evidence="ECO:0000259|Pfam:PF02728" FT DOMAIN 275..696 FT /note="Cu_amine_oxid" FT /evidence="ECO:0000259|Pfam:PF01179" FT ACT_SITE 354 FT /note="Proton acceptor" FT /evidence="ECO:0000256|PIRSR:PIRSR600269-50" FT ACT_SITE 442 FT /note="Schiff-base intermediate with substrate; via FT topaquinone" FT /evidence="ECO:0000256|PIRSR:PIRSR600269-50" FT MOD_RES 442 FT /note="2',4',5'-topaquinone" FT /evidence="ECO:0000256|PIRSR:PIRSR600269-51" SQ SEQUENCE 712 AA; 79856 MW; 6B6101D3C10B67AA CRC64; MAQPSFARLS LLFFGFLLLF ATYSWVFGPD SGFLSGTRVR KTLGSDPKLR VDHSSDKPHH PLDPLTVREI ERVRSILSGH DPDFRSGSAT IHSMALDEPD KARVVRWKKG NRLPSRRAEV VALSGGKSHV ITVDLESGRV VSDLINPSSG YPILTMDDLI AASQVPFKSI EFNRSIEARG VKFSDLVCIT PFAGWFGPEE EGRRIIRVQC YTSKDTPNYF MRPLEGLYAM VDMNKLEVIK VVDKGPVPIP KAAGTEYRYS VLNKPVHMDP INPISMEQPG GPSFRVEGGH LVKWANWVFH VKADQRAGMI ISQATVRDSE TGEPRSVMYK GFPSELFVPY MDPEELWYYK TYMDAGELGL GPPAMPLVPL NDCPRNAYYI DGIFASPDGK AIVQPNMICL FERYAGDVSW RHSEILIPNV DIKEARPKVT LVARMATSVG NYDYIVDWEF QTDGLIRVTV AASGMLMVKG TPYENVDDLG DKEDDSGPLI SENVIGVVHD HFITFHLDMD IDGHTNNSLV KFHLEKQRIP TGKSPRKSYL KVKKYVAKTE KDAQIKLNLY DPYEFHIVNP NRKSRIGNPA GYKIVPGGNA ASLLDHDDPP QIRGAFTNNQ IWVTQYNRSE QFAGGVLVYQ SHGDDTLQVW SDRDRSIENK DIVLWYTLGF HHVPCQEDYP VMPTVAASFE LKPANFFETN PILGVAPFLE KDLPVCRPFA SS //