ID U7TAQ3_FUSNU Unreviewed; 148 AA. AC U7TAQ3; DT 22-JAN-2014, integrated into UniProtKB/TrEMBL. DT 22-JAN-2014, sequence version 1. DT 07-OCT-2020, entry version 35. DE RecName: Full=SsrA-binding protein {ECO:0000256|HAMAP-Rule:MF_00023}; DE AltName: Full=Small protein B {ECO:0000256|HAMAP-Rule:MF_00023}; GN Name=smpB {ECO:0000256|HAMAP-Rule:MF_00023}; GN ORFNames=HMPREF1538_01489 {ECO:0000313|EMBL:ERT41007.1}; OS Fusobacterium nucleatum CTI-1. OC Bacteria; Fusobacteria; Fusobacteriales; Fusobacteriaceae; Fusobacterium. OX NCBI_TaxID=1204474 {ECO:0000313|EMBL:ERT41007.1, ECO:0000313|Proteomes:UP000017136}; RN [1] {ECO:0000313|EMBL:ERT41007.1, ECO:0000313|Proteomes:UP000017136} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=CTI-1 {ECO:0000313|EMBL:ERT41007.1, RC ECO:0000313|Proteomes:UP000017136}; RG The Broad Institute Genomics Platform; RA Earl A., Ward D., Feldgarden M., Gevers D., Kostic A., Garrett W., RA Young S.K., Zeng Q., Gargeya S., Fitzgerald M., Abouelleil A., Alvarado L., RA Berlin A.M., Chapman S.B., Gainer-Dewar J., Goldberg J., Gnerre S., RA Griggs A., Gujja S., Hansen M., Howarth C., Imamovic A., Ireland A., RA Larimer J., McCowan C., Murphy C., Pearson M., Poon T.W., Priest M., RA Roberts A., Saif S., Shea T., Sykes S., Wortman J., Nusbaum C., Birren B.; RT "The Genome Sequence of Fusobacterium nucleatum CTI-1."; RL Submitted (OCT-2013) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Required for rescue of stalled ribosomes mediated by trans- CC translation. Binds to transfer-messenger RNA (tmRNA), required for CC stable association of tmRNA with ribosomes. tmRNA and SmpB together CC mimic tRNA shape, replacing the anticodon stem-loop with SmpB. tmRNA is CC encoded by the ssrA gene; the 2 termini fold to resemble tRNA(Ala) and CC it encodes a 'tag peptide', a short internal open reading frame. During CC trans-translation Ala-aminoacylated tmRNA acts like a tRNA, entering CC the A-site of stalled ribosomes, displacing the stalled mRNA. The CC ribosome then switches to translate the ORF on the tmRNA; the nascent CC peptide is terminated with the 'tag peptide' encoded by the tmRNA and CC targeted for degradation. The ribosome is freed to recommence CC translation, which seems to be the essential function of trans- CC translation. {ECO:0000256|HAMAP-Rule:MF_00023}. CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00023}. CC Note=The tmRNA-SmpB complex associates with stalled 70S ribosomes. CC {ECO:0000256|HAMAP-Rule:MF_00023}. CC -!- SIMILARITY: Belongs to the SmpB family. {ECO:0000256|HAMAP- CC Rule:MF_00023}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:ERT41007.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AXNZ01000016; ERT41007.1; -; Genomic_DNA. DR RefSeq; WP_023040117.1; NZ_KI518461.1. DR EnsemblBacteria; ERT41007; ERT41007; HMPREF1538_01489. DR PATRIC; fig|1204474.3.peg.1454; -. DR HOGENOM; CLU_108953_0_1_0; -. DR Proteomes; UP000017136; Unassembled WGS sequence. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule. DR GO; GO:0070929; P:trans-translation; IEA:UniProtKB-UniRule. DR CDD; cd09294; SmpB; 1. DR Gene3D; 2.40.280.10; -; 1. DR HAMAP; MF_00023; SmpB; 1. DR InterPro; IPR023620; SmpB. DR InterPro; IPR000037; SsrA-bd_prot. DR InterPro; IPR020081; SsrA-bd_prot_CS. DR PANTHER; PTHR30308; PTHR30308; 1. DR Pfam; PF01668; SmpB; 1. DR SUPFAM; SSF74982; SSF74982; 1. DR TIGRFAMs; TIGR00086; smpB; 1. DR PROSITE; PS01317; SSRP; 1. PE 3: Inferred from homology; KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_00023}; KW RNA-binding {ECO:0000256|HAMAP-Rule:MF_00023}. FT REGION 128..148 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" SQ SEQUENCE 148 AA; 17359 MW; C5B5C424707730A2 CRC64; MIIANNKKAF FDYFIEEKYE AGIELKGSEV KSIKAGKVSI KESFVRIIND EIFIMGMSVV PWDFGSVYNP EERRVRKLLL HRKEIKKIHE KVKIKGYTIV PLDVHLSKGY VKIQIAIAKG KKTYDKRESI AKKDQERNLK REFKNNNR //