ID U2NUA0_LACBR Unreviewed; 159 AA. AC U2NUA0; DT 13-NOV-2013, integrated into UniProtKB/TrEMBL. DT 13-NOV-2013, sequence version 1. DT 15-MAR-2017, entry version 24. DE RecName: Full=Ribosomal RNA large subunit methyltransferase H {ECO:0000256|HAMAP-Rule:MF_00658, ECO:0000256|SAAS:SAAS00702567}; DE EC=2.1.1.177 {ECO:0000256|HAMAP-Rule:MF_00658, ECO:0000256|SAAS:SAAS00702569}; DE AltName: Full=23S rRNA (pseudouridine1915-N3)-methyltransferase {ECO:0000256|HAMAP-Rule:MF_00658}; DE AltName: Full=23S rRNA m3Psi1915 methyltransferase {ECO:0000256|HAMAP-Rule:MF_00658}; DE AltName: Full=rRNA (pseudouridine-N3-)-methyltransferase RlmH {ECO:0000256|HAMAP-Rule:MF_00658}; GN Name=rlmH {ECO:0000256|HAMAP-Rule:MF_00658}; GN ORFNames=HMPREF0495_02174 {ECO:0000313|EMBL:ERK41610.1}; OS Lactobacillus brevis ATCC 14869 = DSM 20054. OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; OC Lactobacillus. OX NCBI_TaxID=649758 {ECO:0000313|EMBL:ERK41610.1, ECO:0000313|Proteomes:UP000016644}; RN [1] {ECO:0000313|EMBL:ERK41610.1, ECO:0000313|Proteomes:UP000016644} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 14869 {ECO:0000313|EMBL:ERK41610.1, RC ECO:0000313|Proteomes:UP000016644}; RA Weinstock G., Sodergren E., Lobos E.A., Fulton L., Fulton R., RA Courtney L., Fronick C., O'Laughlin M., Godfrey J., Wilson R.M., RA Miner T., Farmer C., Delehaunty K., Cordes M., Minx P., Tomlinson C., RA Chen J., Wollam A., Pepin K.H., Bhonagiri V., Zhang X., Warren W., RA Mitreva M., Mardis E.R., Wilson R.K.; RL Submitted (JUN-2013) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Specifically methylates the pseudouridine at position CC 1915 (m3Psi1915) in 23S rRNA. {ECO:0000256|HAMAP-Rule:MF_00658, CC ECO:0000256|SAAS:SAAS00702576}. CC -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + pseudouridine(1915) CC in 23S rRNA = S-adenosyl-L-homocysteine + N(3)- CC methylpseudouridine(1915) in 23S rRNA. {ECO:0000256|HAMAP- CC Rule:MF_00658, ECO:0000256|SAAS:SAAS00702568}. CC -!- SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_00658, CC ECO:0000256|SAAS:SAAS00702563}. CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00658, CC ECO:0000256|SAAS:SAAS00702564}. CC -!- SIMILARITY: Belongs to the RNA methyltransferase RlmH family. CC {ECO:0000256|HAMAP-Rule:MF_00658, ECO:0000256|SAAS:SAAS00702575}. CC -!- CAUTION: The sequence shown here is derived from an CC EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is CC preliminary data. {ECO:0000313|EMBL:ERK41610.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AWVK01000106; ERK41610.1; -; Genomic_DNA. DR RefSeq; WP_011666849.1; NZ_KI271219.1. DR ProteinModelPortal; U2NUA0; -. DR SMR; U2NUA0; -. DR EnsemblBacteria; ERK41610; ERK41610; HMPREF0495_02174. DR EnsemblBacteria; KRK20425; KRK20425; FC61_GL001200. DR GeneID; 4414532; -. DR OrthoDB; POG091H028B; -. DR Proteomes; UP000016644; Unassembled WGS sequence. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0070038; F:rRNA (pseudouridine-N3-)-methyltransferase activity; IEA:UniProtKB-HAMAP. DR Gene3D; 3.40.1280.10; -; 1. DR HAMAP; MF_00658; 23SrRNA_methyltr_H; 1. DR InterPro; IPR029028; Alpha/beta_knot_MTases. DR InterPro; IPR003742; RlmH-like. DR InterPro; IPR029026; tRNA_m1G_MTases_N. DR PANTHER; PTHR33603:SF2; PTHR33603:SF2; 1. DR Pfam; PF02590; SPOUT_MTase; 1. DR PIRSF; PIRSF004505; MT_bac; 1. DR SUPFAM; SSF75217; SSF75217; 1. DR TIGRFAMs; TIGR00246; tRNA_RlmH_YbeA; 1. PE 3: Inferred from homology; KW Complete proteome {ECO:0000313|Proteomes:UP000016644}; KW Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00658, KW ECO:0000256|SAAS:SAAS00702578}; KW Methyltransferase {ECO:0000256|HAMAP-Rule:MF_00658, KW ECO:0000256|SAAS:SAAS00702579, ECO:0000313|EMBL:ERK41610.1}; KW rRNA processing {ECO:0000256|HAMAP-Rule:MF_00658, KW ECO:0000256|SAAS:SAAS00702566}; KW S-adenosyl-L-methionine {ECO:0000256|HAMAP-Rule:MF_00658, KW ECO:0000256|SAAS:SAAS00702581}; KW Transferase {ECO:0000256|HAMAP-Rule:MF_00658, KW ECO:0000256|SAAS:SAAS00702565, ECO:0000313|EMBL:ERK41610.1}. SQ SEQUENCE 159 AA; 18113 MW; 6B704A3DFCAC165A CRC64; MNIKFVVVGK LKEKYFKQGI AEYAKRLSRF CKFSIVEVPD EKAPENLSQA EMDRVMAEEG ERILAKIKDR EYVYALAILG KERSSEAFAK EIKDLTTYGH SDITFVIGGS LGLTPAVLKR ADTQISFGKF TLPHQLMRLV LTEQVYRAFM INEGSPYHK //