ID   T5HGY5_BACLI            Unreviewed;      1282 AA.
AC   T5HGY5;
DT   13-NOV-2013, integrated into UniProtKB/TrEMBL.
DT   13-NOV-2013, sequence version 1.
DT   02-JUN-2021, entry version 35.
DE   SubName: Full=Peptide synthetase {ECO:0000313|EMBL:EQM24881.1};
GN   ORFNames=N399_01990 {ECO:0000313|EMBL:EQM24881.1};
OS   Bacillus licheniformis CG-B52.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=1368424 {ECO:0000313|EMBL:EQM24881.1, ECO:0000313|Proteomes:UP000017025};
RN   [1] {ECO:0000313|Proteomes:UP000017025}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CG-B52 {ECO:0000313|Proteomes:UP000017025};
RA   Galvez-Bobadilla E.J., Zarate-Bonilla L.J., Carrillo-Castro K., Guiza L.,
RA   Pieper D.H., Salazar M., Junca H.;
RT   "Draft genome of a Bacillus sp. strain CG-B52 virulent to shrimps.";
RL   Submitted (AUG-2013) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:EQM24881.1, ECO:0000313|Proteomes:UP000017025}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CG-B52 {ECO:0000313|EMBL:EQM24881.1,
RC   ECO:0000313|Proteomes:UP000017025};
RX   PubMed=27174263;
RA   Galvez E.J., Carrillo-Castro K., Zarate L., Guiza L., Pieper D.H.,
RA   Garcia-Bonilla E., Salazar M., Junca H.;
RT   "Draft Genome Sequence of Bacillus licheniformis CG-B52, a Highly Virulent
RT   Bacterium of Pacific White Shrimp (Litopenaeus vannamei), Isolated from a
RT   Colombian Caribbean Aquaculture Outbreak.";
RL   Genome Announc. 4:0-0(2016).
CC   -!- COFACTOR:
CC       Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC         Evidence={ECO:0000256|ARBA:ARBA00001957};
CC   -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family.
CC       {ECO:0000256|ARBA:ARBA00006432}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:EQM24881.1}.
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DR   EMBL; AVEZ01000049; EQM24881.1; -; Genomic_DNA.
DR   RefSeq; WP_003178834.1; NZ_AVEZ01000049.1.
DR   ESTHER; bacli-LICC; Thioesterase.
DR   EnsemblBacteria; EQM24881; EQM24881; N399_01990.
DR   GeneID; 3029681; -.
DR   PATRIC; fig|1368424.3.peg.4708; -.
DR   Proteomes; UP000017025; Unassembled WGS sequence.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR   GO; GO:0009058; P:biosynthetic process; IEA:InterPro.
DR   Gene3D; 1.10.1200.10; -; 1.
DR   Gene3D; 3.30.559.10; -; 1.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR010071; AA_adenyl_domain.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR036736; ACP-like_sf.
DR   InterPro; IPR025110; AMP-bd_C.
DR   InterPro; IPR020845; AMP-binding_CS.
DR   InterPro; IPR000873; AMP-dep_Synth/Lig.
DR   InterPro; IPR023213; CAT-like_dom_sf.
DR   InterPro; IPR001242; Condensatn.
DR   InterPro; IPR020806; PKS_PP-bd.
DR   InterPro; IPR009081; PP-bd_ACP.
DR   InterPro; IPR006162; Ppantetheine_attach_site.
DR   InterPro; IPR001031; Thioesterase.
DR   Pfam; PF00501; AMP-binding; 1.
DR   Pfam; PF13193; AMP-binding_C; 1.
DR   Pfam; PF00668; Condensation; 1.
DR   Pfam; PF00550; PP-binding; 1.
DR   Pfam; PF00975; Thioesterase; 1.
DR   SMART; SM00823; PKS_PP; 1.
DR   SUPFAM; SSF47336; SSF47336; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
DR   TIGRFAMs; TIGR01733; AA-adenyl-dom; 1.
DR   PROSITE; PS00455; AMP_BINDING; 1.
DR   PROSITE; PS50075; CARRIER; 1.
DR   PROSITE; PS00012; PHOSPHOPANTETHEINE; 1.
PE   3: Inferred from homology;
KW   Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450, ECO:0000256|PROSITE-
KW   ProRule:PRU00258}; Phosphoprotein {ECO:0000256|ARBA:ARBA00022553}.
FT   DOMAIN          973..1048
FT                   /note="Carrier"
FT                   /evidence="ECO:0000259|PROSITE:PS50075"
FT   REGION          957..977
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        958..977
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         1008
FT                   /note="O-(pantetheine 4'-phosphoryl)serine"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00258"
SQ   SEQUENCE   1282 AA;  144200 MW;  1C94012B4EFF2DE3 CRC64;
     MSQFKKEHVQ DIYYLSPMQE GMLFHTLLHP GQGFYIEQIS MQVKGSFQKD VLEKSMNVII
     GRYDIFRTVF VHEKMKRPVQ VVLKERSFQA EEIDLSGLSE AEQNERIEDY KRKDKEKGFN
     LSKDIPMRTA VFKKGQDRYE WVWSYHHILL DGWCFGIVVQ ELFEVYNALR ENRPYSLGPV
     RPYKEYIKWL ESQDKQKSLV YWEHHLAGFE GQTTFSEQRK NPEQAGNQPE ELLFTLPKED
     TEAFTQLAKA HHTTLSTALQ AVWSVLLSRY QRSRDLIFGT VVSGRPADIQ GVEHMVGLFI
     NVVPKRATFG PQTTFTELVA DLQKQALEAE PHQYVPLYEI QSHIAAPNLI DHIIVFENYP
     LQEANKQQKE KNLGFTMDEI SVFEKSNYDL NLLASPGEEM MLKLAYNRNI FEPSFILRLK
     EQLLTAIREV IRDPEQPLDD ICVVSKKEKE RLLHEFNGQV QRKQIGLTIP QWFEQYAEAL
     PEKPAVSAAG RTLTYRELNE KANKVAHLLR KKNVGRGEPV ALLFRRSPEM VIAILAVLKA
     GGAYLPIDPE YPEARIQYML EDSGAVCMLT QEELAGRAAS LSFHKHTILI DDPAVSAESG
     RNLEIAAEPD DLAYIMYTSG TTGKPKGNLT THANITRVVK ETNYISLSEK DTLLSLSNYA
     FDGFTFDLYG ALLNGAKLVV ADQATILHIG KLTEAIQKEN ITVMFVTTAL FNLLVDAGTG
     WMKGIRKVLF GGERSSVSHV KKAFAAMGPD RIIHVYGPTE TTVFATFYPV NRMEDSAVSI
     PIGKPINETN AYILTENNRL QPIGAVGELC LSGTGVSRGY LNRPELTAEK FAPHPFNSGE
     TMYRTGDLAR WLPDGNIDFI GRIDDQVKIR GHRIELGEIE EQLMRCQGVK EAVVTARKSG
     NGDAALTAYV VPVQGTEVAN EEVRRQLARR LPAYMVPAAY IMLEELPLTA NGKVNRRLLP
     EADGRPNSTE RRAPRNATEE KLAVIWSEVL GRQQIGIDEN FFEIGGHSLK AMAVASRMLK
     DLAIDVPVNV LFEMPTIEGL AAYIDQGGHV DEVKKTVFNQ ASTRHLFAFP PVLGYGIMYG
     KLAEQMPEYR IHAFDFIESD DRAVRYAEAI SDLQPEGPLT LIGYSAGCGL AFEVAQALEK
     NGRKVEKLLM IDSYMKNDVS DLEGRSIEAD TAALMEANKD NEYMKIKTVA DGIAKKMTAY
     YSYFVNLVHQ GTVSSEIHLI KSEHAKPLPA WLSLWKEGTE SSYDEYQGAG RHDEMLADGF
     VKSNADLIRQ ILGERAAVIG GA
//