ID LAEA_GIBF5 Reviewed; 316 AA. AC S0DQI7; E0WDF6; DT 16-MAR-2016, integrated into UniProtKB/Swiss-Prot. DT 16-MAR-2016, sequence version 2. DT 16-JAN-2019, entry version 21. DE RecName: Full=Secondary metabolism regulator LAE1 {ECO:0000305}; DE AltName: Full=Methyltransferase LAE1 {ECO:0000305}; DE EC=2.1.1.- {ECO:0000250|UniProtKB:C8VQG9}; DE AltName: Full=Velvet complex subunit LAE1 {ECO:0000305}; GN Name=LAE1 {ECO:0000303|PubMed:20572938}; ORFNames=FFUJ_00592; OS Gibberella fujikuroi (strain CBS 195.34 / IMI 58289 / NRRL A-6831) OS (Bakanae and foot rot disease fungus) (Fusarium fujikuroi). OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; OC Sordariomycetes; Hypocreomycetidae; Hypocreales; Nectriaceae; OC Fusarium; Fusarium fujikuroi species complex. OX NCBI_TaxID=1279085; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION, SUBCELLULAR LOCATION, RP INTERACTION WITH VEL1/VEA, AND DISRUPTION PHENOTYPE. RC STRAIN=CBS 195.34 / IMI 58289 / NRRL A-6831; RX PubMed=20572938; DOI=10.1111/j.1365-2958.2010.07263.x; RA Wiemann P., Brown D.W., Kleigrewe K., Bok J.W., Keller N.P., RA Humpf H.U., Tudzynski B.; RT "FfVel1 and FfLae1, components of a velvet-like complex in Fusarium RT fujikuroi, affect differentiation, secondary metabolism and RT virulence."; RL Mol. Microbiol. 77:972-994(2010). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=CBS 195.34 / IMI 58289 / NRRL A-6831; RX PubMed=23825955; DOI=10.1371/journal.ppat.1003475; RA Wiemann P., Sieber C.M.K., von Bargen K.W., Studt L., Niehaus E.-M., RA Espino J.J., Huss K., Michielse C.B., Albermann S., Wagner D., RA Bergner S.V., Connolly L.R., Fischer A., Reuter G., Kleigrewe K., RA Bald T., Wingfield B.D., Ophir R., Freeman S., Hippler M., Smith K.M., RA Brown D.W., Proctor R.H., Muensterkoetter M., Freitag M., Humpf H.-U., RA Gueldener U., Tudzynski B.; RT "Deciphering the cryptic genome: genome-wide analyses of the rice RT pathogen Fusarium fujikuroi reveal complex regulation of secondary RT metabolism and novel metabolites."; RL PLoS Pathog. 9:E1003475-E1003475(2013). RN [3] RP FUNCTION, AND DISRUPTION PHENOTYPE. RX PubMed=24389666; DOI=10.1007/s00253-013-5453-1; RA Niehaus E.M., von Bargen K.W., Espino J.J., Pfannmueller A., RA Humpf H.U., Tudzynski B.; RT "Characterization of the fusaric acid gene cluster in Fusarium RT fujikuroi."; RL Appl. Microbiol. Biotechnol. 98:1749-1762(2014). CC -!- FUNCTION: Methyltransferase that performs auto-methylation (By CC similarity). No other methyl-accepting substrate has been CC identified yet (By similarity). Component of the velvet CC transcription factor complex that acts as a global regulator for CC secondary metabolite gene expression (PubMed:20572938). Controls CC the expression of the gibberellins gene clusters, but does not CC affect bikaverin production (PubMed:20572938). Controls the CC expression of the fusaric acid gene cluster (PubMed:24389666). CC Acts as a virulence factors during infection, most likely through CC activation of gibberellins biosynthesis (PubMed:20572938). CC {ECO:0000250|UniProtKB:C8VQG9, ECO:0000269|PubMed:20572938, CC ECO:0000269|PubMed:24389666}. CC -!- SUBUNIT: Component of the heterotrimeric velvet complex composed CC of LAE1, VEL1 and VEL2; VEL1 acting as a bridging protein between CC LAE1 and VEL2 (By similarity). Interacts with VEL1 CC (PubMed:20572938). {ECO:0000250|UniProtKB:C8VQG9, CC ECO:0000269|PubMed:20572938}. CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:20572938}. CC -!- INDUCTION: Expression is regulated by the VEL1 (PubMed:20572938). CC {ECO:0000269|PubMed:20572938}. CC -!- DISRUPTION PHENOTYPE: Reduces gibberellins production and CC subsequent virulence during infection (PubMed:20572938). Reduces CC both the fusaric acid gene cluster expression and production of CC fusaric acid (PubMed:24389666). {ECO:0000269|PubMed:20572938, CC ECO:0000269|PubMed:24389666}. CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. LaeA CC methyltransferase family. {ECO:0000305}. CC -!- SEQUENCE CAUTION: CC Sequence=CCT62818.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; FN548141; CBE54370.1; -; Genomic_DNA. DR EMBL; HF679023; CCT62818.1; ALT_SEQ; Genomic_DNA. DR ProteinModelPortal; S0DQI7; -. DR EnsemblFungi; CCT62818; CCT62818; FFUJ_00592. DR Proteomes; UP000016800; Chromosome 1. DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell. DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW. DR GO; GO:0009405; P:pathogenesis; IEA:UniProtKB-KW. DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW. DR InterPro; IPR029063; SAM-dependent_MTases. DR SUPFAM; SSF53335; SSF53335; 1. PE 1: Evidence at protein level; KW Complete proteome; Methyltransferase; Nucleus; Reference proteome; KW S-adenosyl-L-methionine; Sporulation; Transcription; KW Transcription regulation; Transferase; Virulence. FT CHAIN 1 316 Secondary metabolism regulator LAE1. FT /FTId=PRO_0000435749. SQ SEQUENCE 316 AA; 36624 MW; 4657FCFE4FFDB2DA CRC64; MVVMPPQNSV NESEGRYLQD GFWQHGRFYG SWKPGKYLFP IDSEELNRLD IFHKVFLLAR DNKPFLAPIR RTSPRIMDIG TGTGIWAINV AEECLSDAQI MAVDLNQIQP ALIPPGFMPK QYDIEEPSWG PLLADCDLIH MRMLLGSIQT DLWPQVYHNA FEHLTPGIGF LEHIEVDWIP RCDDDERPAN SAFVKWAELF LDGMDRFNRS VRVIPQEHRQ MLEATGFTDV KQEVIKAYVC PWSADRNERE IARWFNIGLS HSLEAMSLKP LIEKLGFEAE DVRELCERAK RETCVLRYHT YCNIHVWTAR KPGPQQ //