ID PB27B_ARATH Reviewed; 199 AA. AC Q9ZVZ9; Q8GXE0; DT 24-JUL-2013, integrated into UniProtKB/Swiss-Prot. DT 01-MAY-1999, sequence version 1. DT 08-JUN-2016, entry version 83. DE RecName: Full=Photosystem II D1 precursor processing protein PSB27-H2, chloroplastic; DE Short=Psb27-H2; DE AltName: Full=LOW PSII accumulation 19 protein; DE Short=LPA19; DE AltName: Full=Thylakoid lumenal protein PSB27-H2; DE Flags: Precursor; GN Name=PSB27-2; Synonyms=LPA19; OrderedLocusNames=At1g05385; GN ORFNames=T25N20.4; OS Arabidopsis thaliana (Mouse-ear cress). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae; OC Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; OC Arabidopsis. OX NCBI_TaxID=3702; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Columbia; RX PubMed=11130712; DOI=10.1038/35048500; RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., RA White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., RA Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., RA Chung M.K., Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., RA Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., RA Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., RA Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., RA Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., RA Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., RA Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., RA Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., RA Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., RA Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., RA Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., RA Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.; RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis RT thaliana."; RL Nature 408:816-820(2000). RN [2] RP GENOME REANNOTATION. RC STRAIN=cv. Columbia; RG The Arabidopsis Information Resource (TAIR); RL Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases. RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=cv. Columbia; RX PubMed=14593172; DOI=10.1126/science.1088305; RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., RA Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., RA Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G., RA Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., RA Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., RA Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., RA Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., RA Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., RA Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., RA Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., RA Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., RA Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.; RT "Empirical analysis of transcriptional activity in the Arabidopsis RT genome."; RL Science 302:842-846(2003). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=cv. Columbia; RX PubMed=11910074; DOI=10.1126/science.1071006; RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., RA Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., RA Shibata K., Shinagawa A., Shinozaki K.; RT "Functional annotation of a full-length Arabidopsis cDNA collection."; RL Science 296:141-145(2002). RN [5] RP FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH D1 (PSBA), AND RP DISRUPTION PHENOTYPE. RC STRAIN=cv. Columbia; RX PubMed=20444695; DOI=10.1074/jbc.M110.105064; RA Wei L., Guo J., Ouyang M., Sun X., Ma J., Chi W., Lu C., Zhang L.; RT "LPA19, a Psb27 homolog in Arabidopsis thaliana, facilitates D1 RT protein precursor processing during PSII biogenesis."; RL J. Biol. Chem. 285:21391-21398(2010). CC -!- FUNCTION: Required, but not essential, for D1 (psbA) precursor CC processing and thus correct photosystem II assembly (PSII). CC {ECO:0000269|PubMed:20444695}. CC -!- SUBUNIT: Interacts with the C-terminus of both the precursor and CC mature form of D1. {ECO:0000269|PubMed:20444695}. CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid lumen CC {ECO:0000269|PubMed:20444695}. Note=Associated with PSII on the CC lumenal side of the thylakoid membrane. CC -!- DISRUPTION PHENOTYPE: A high chlorophyll fluorescence mutant. CC Impaired PSII function, maximum photochemical efficiency of PSII CC was decreased, about 25% PSII in thylakoids; severely reduced CC growth, decreased chlorophyll content. Inefficient processing of CC the D1 precursor to the mature form. CC {ECO:0000269|PubMed:20444695}. CC -!- SIMILARITY: Belongs to the Psb27 family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AC005106; AAF79740.1; -; Genomic_DNA. DR EMBL; CP002684; AEE27833.1; -; Genomic_DNA. DR EMBL; AF370242; AAK44057.1; -; mRNA. DR EMBL; AY142580; AAN13149.1; -; mRNA. DR EMBL; AK118288; BAC42906.1; -; mRNA. DR PIR; G86188; G86188. DR RefSeq; NP_563737.1; NM_100418.2. DR UniGene; At.15340; -. DR ProteinModelPortal; Q9ZVZ9; -. DR SMR; Q9ZVZ9; 94-196. DR BioGrid; 22282; 1. DR STRING; 3702.AT1G05385.1; -. DR PaxDb; Q9ZVZ9; -. DR PRIDE; Q9ZVZ9; -. DR EnsemblPlants; AT1G05385.1; AT1G05385.1; AT1G05385. DR GeneID; 837040; -. DR Gramene; AT1G05385.1; AT1G05385.1; AT1G05385. DR KEGG; ath:AT1G05385; -. DR TAIR; AT1G05385; -. DR eggNOG; ENOG410IYA6; Eukaryota. DR eggNOG; ENOG4111U0T; LUCA. DR HOGENOM; HOG000240416; -. DR InParanoid; Q9ZVZ9; -. DR OMA; KFYSKAG; -. DR PhylomeDB; Q9ZVZ9; -. DR PRO; PR:Q9ZVZ9; -. DR Proteomes; UP000006548; Chromosome 1. DR Genevisible; Q9ZVZ9; AT. DR GO; GO:0009507; C:chloroplast; IDA:TAIR. DR GO; GO:0009543; C:chloroplast thylakoid lumen; IDA:TAIR. DR GO; GO:0010207; P:photosystem II assembly; IMP:TAIR. DR GO; GO:0010206; P:photosystem II repair; IBA:GO_Central. DR HAMAP; MF_01481; PSII_Psb27; 1. DR InterPro; IPR025585; PSII_Pbs27. DR Pfam; PF13326; PSII_Pbs27; 1. PE 1: Evidence at protein level; KW Chloroplast; Complete proteome; Plastid; Reference proteome; KW Thylakoid; Transit peptide. FT TRANSIT 1 ? Chloroplast. {ECO:0000255}. FT TRANSIT ? 64 Thylakoid. {ECO:0000255}. FT CHAIN 65 199 Photosystem II D1 precursor processing FT protein PSB27-H2, chloroplastic. FT /FTId=PRO_0000422926. FT CONFLICT 122 122 K -> R (in Ref. 4; BAC42906). FT {ECO:0000305}. SQ SEQUENCE 199 AA; 22267 MW; 5A36A68A408EB73C CRC64; MGFLVAVMNF SPTLVHHHMK SKPQCQNEKL RQGQTSSLFD RRGFLKCVVG ASSFMATIEF SGLQAQASEE KLDEGEGVVG AFKTLFDPNE RTKSGKELPK AYLKSAREVV KTMRESLKEN PKDNAKFRRS ADAAKESIRD YLSNWRGQKT VAGEESYVEL ENVIRALAKF YSKAGPSAPL PDEVKTEILD DLNKAEEFL //