ID   HM20B_MOUSE             Reviewed;         317 AA.
AC   Q9Z104; Q9NSF7; Q9NSF8;
DT   04-JAN-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   02-DEC-2020, entry version 158.
DE   RecName: Full=SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1-related;
DE            Short=SMARCE1-related protein;
DE   AltName: Full=BRCA2-associated factor 35;
DE   AltName: Full=HMG box-containing protein 20B;
DE   AltName: Full=Structural DNA-binding protein BRAF35;
GN   Name=Hmg20b; Synonyms=Braf35, Hmgx2, Smarce1r;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=10486208; DOI=10.1006/geno.1999.5913;
RA   Wattler F., Wattler S., Kelly M., Skinner H.B., Nehls M.;
RT   "Cloning, chromosomal location, and expression analysis of murine Smarce1-
RT   related, a new member of the high-mobility 365 group gene family.";
RL   Genomics 60:172-178(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=10773667; DOI=10.1159/000015486;
RA   Sumoy L., Carim-Todd L., Escarceller M., Nadal M., Gratacos M.,
RA   Pujana M.A., Estivill X., Peral B.;
RT   "HMG20A and HMG20B map to human chromosomes 15q24 and 19p13.3 and
RT   constitute a distinct class of HMG-box genes with ubiquitous expression.";
RL   Cytogenet. Cell Genet. 88:62-67(2000).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Czech II; TISSUE=Mammary tumor;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   STRUCTURE BY NMR OF 69-148.
RG   RIKEN structural genomics initiative (RSGI);
RT   "Solution structure of the HMG domain of mouse HMG domain protein HMGX2.";
RL   Submitted (NOV-2005) to the PDB data bank.
CC   -!- FUNCTION: Required for correct progression through G2 phase of the cell
CC       cycle and entry into mitosis. Required for RCOR1/CoREST mediated
CC       repression of neuronal specific gene promoters (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Component of a BHC histone deacetylase complex that contains
CC       HDAC1, HDAC2, HMG20B/BRAF35, KDM1A, RCOR1/CoREST and PHF21A/BHC80. The
CC       BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I.
CC       Interacts with the BRCA2 tumor suppressor protein (By similarity).
CC       {ECO:0000250}.
CC   -!- INTERACTION:
CC       Q9Z104; P39428: Traf1; NbExp=3; IntAct=EBI-646920, EBI-520123;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00267}.
CC       Chromosome {ECO:0000250}. Note=Localized to condensed chromosomes in
CC       mitosis in conjunction with BRCA2. {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Widely expressed in adult tissues, particularly
CC       kidney, skin, testis and uterus. Highly expressed in embryonic tissues
CC       with high mitotic index, such as the proliferating ventricular zones of
CC       the fore-, mid- and hindbrain.
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DR   EMBL; AF067430; AAD15897.1; -; Genomic_DNA.
DR   EMBL; AF146224; AAF66708.1; -; mRNA.
DR   EMBL; AL355734; CAB90813.1; -; mRNA.
DR   EMBL; AL355735; CAB90814.1; -; mRNA.
DR   EMBL; BC011334; AAH11334.1; -; mRNA.
DR   CCDS; CCDS24055.1; -.
DR   RefSeq; NP_001156637.1; NM_001163165.1.
DR   RefSeq; NP_001156638.1; NM_001163166.1.
DR   RefSeq; NP_034570.1; NM_010440.3.
DR   PDB; 2CRJ; NMR; -; A=70-148.
DR   PDBsum; 2CRJ; -.
DR   SMR; Q9Z104; -.
DR   BioGRID; 200335; 6.
DR   IntAct; Q9Z104; 1.
DR   STRING; 10090.ENSMUSP00000020454; -.
DR   iPTMnet; Q9Z104; -.
DR   PhosphoSitePlus; Q9Z104; -.
DR   EPD; Q9Z104; -.
DR   MaxQB; Q9Z104; -.
DR   PaxDb; Q9Z104; -.
DR   PRIDE; Q9Z104; -.
DR   Antibodypedia; 23254; 287 antibodies.
DR   Ensembl; ENSMUST00000020454; ENSMUSP00000020454; ENSMUSG00000020232.
DR   Ensembl; ENSMUST00000105323; ENSMUSP00000100960; ENSMUSG00000020232.
DR   Ensembl; ENSMUST00000105324; ENSMUSP00000100961; ENSMUSG00000020232.
DR   Ensembl; ENSMUST00000167481; ENSMUSP00000128807; ENSMUSG00000020232.
DR   GeneID; 15353; -.
DR   KEGG; mmu:15353; -.
DR   UCSC; uc007ghj.2; mouse.
DR   CTD; 10362; -.
DR   MGI; MGI:1341190; Hmg20b.
DR   eggNOG; KOG0381; Eukaryota.
DR   GeneTree; ENSGT00940000161213; -.
DR   InParanoid; Q9Z104; -.
DR   OMA; KIKQRWT; -.
DR   OrthoDB; 1458939at2759; -.
DR   PhylomeDB; Q9Z104; -.
DR   TreeFam; TF106440; -.
DR   Reactome; R-MMU-3214815; HDACs deacetylate histones.
DR   Reactome; R-MMU-983231; Factors involved in megakaryocyte development and platelet production.
DR   BioGRID-ORCS; 15353; 2 hits in 21 CRISPR screens.
DR   ChiTaRS; Hmg20b; mouse.
DR   EvolutionaryTrace; Q9Z104; -.
DR   PRO; PR:Q9Z104; -.
DR   Proteomes; UP000000589; Chromosome 10.
DR   RNAct; Q9Z104; protein.
DR   Bgee; ENSMUSG00000020232; Expressed in heart left ventricle and 236 other tissues.
DR   Genevisible; Q9Z104; MM.
DR   GO; GO:0002111; C:BRCA2-BRAF35 complex; ISO:MGI.
DR   GO; GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
DR   GO; GO:0016604; C:nuclear body; ISO:MGI.
DR   GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0003677; F:DNA binding; TAS:ProtInc.
DR   GO; GO:0000400; F:four-way junction DNA binding; ISO:MGI.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0006325; P:chromatin organization; NAS:UniProtKB.
DR   GO; GO:0006338; P:chromatin remodeling; TAS:ProtInc.
DR   GO; GO:0033234; P:negative regulation of protein sumoylation; IDA:MGI.
DR   GO; GO:0045666; P:positive regulation of neuron differentiation; IGI:MGI.
DR   GO; GO:0010468; P:regulation of gene expression; IBA:GO_Central.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; NAS:UniProtKB.
DR   GO; GO:0035914; P:skeletal muscle cell differentiation; IMP:MGI.
DR   Gene3D; 1.10.30.10; -; 1.
DR   InterPro; IPR009071; HMG_box_dom.
DR   InterPro; IPR036910; HMG_box_dom_sf.
DR   Pfam; PF00505; HMG_box; 1.
DR   SMART; SM00398; HMG; 1.
DR   SUPFAM; SSF47095; SSF47095; 1.
DR   PROSITE; PS50118; HMG_BOX_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cell cycle; Chromatin regulator; Chromosome; Coiled coil;
KW   DNA-binding; Isopeptide bond; Nucleus; Phosphoprotein; Reference proteome;
KW   Transcription; Transcription regulation; Ubl conjugation.
FT   CHAIN           1..317
FT                   /note="SWI/SNF-related matrix-associated actin-dependent
FT                   regulator of chromatin subfamily E member 1-related"
FT                   /id="PRO_0000048576"
FT   DNA_BIND        70..138
FT                   /note="HMG box"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00267"
FT   COILED          190..257
FT                   /evidence="ECO:0000255"
FT   MOD_RES         160
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9P0W2"
FT   CROSSLNK        31
FT                   /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT                   G-Cter in SUMO2)"
FT                   /evidence="ECO:0000250|UniProtKB:Q9P0W2"
FT   HELIX           76..91
FT                   /evidence="ECO:0000244|PDB:2CRJ"
FT   HELIX           97..109
FT                   /evidence="ECO:0000244|PDB:2CRJ"
FT   HELIX           115..138
FT                   /evidence="ECO:0000244|PDB:2CRJ"
FT   HELIX           141..144
FT                   /evidence="ECO:0000244|PDB:2CRJ"
SQ   SEQUENCE   317 AA;  35870 MW;  6C4D45D2EB1D403B CRC64;
     MSHGPRQPGA ATAPAGGKTP GQHGAFVVAV KQERSEGSRA GEKGPQEEEP VKKRGWPKGK
     KRKKILPNGP KAPVTGYVRF LNERREQIRT RHPDLPFPEI TKMLGAEWSK LQPAEKQRYL
     DEAEKEKQQY LKELWAYQQS EAYKVCTEKI QENKIKKEDS SSGLMNTLLN GHKGVDCDGF
     STFDVPIFTE EFLDQNKARE AELRRLRKMN VAFEEQNAVL QRHTQSMSSA RERLEQELAL
     EERRTLALQQ QLQAVRQALT ASFASLPVPG TGETPTLGTL DFYMARLHGA IERDPAQHER
     LIARVKEILA RVASEHL
//