ID HEMK2_HUMAN Reviewed; 214 AA. AC Q9Y5N5; DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot. DT 01-DEC-2000, sequence version 2. DT 15-JUN-2010, entry version 77. DE RecName: Full=HemK methyltransferase family member 2; DE EC=2.1.1.-; DE AltName: Full=N(6)-adenine-specific DNA methyltransferase 1; DE AltName: Full=M.HsaHemK2P; GN Name=N6AMT1; Synonyms=C21orf127, HEMK2; ORFNames=PRED28; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; OC Catarrhini; Hominidae; Homo. OX NCBI_TaxID=9606; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA], AND VARIANTS ARG-146 AND ARG-166. RA Reboul J., Misseri Y., Bonnerot C., Mogensen E., Lutfalla G.; RT "Identification of a novel putative eukaryotic DNA- RT methyltransferase."; RL Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases. RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RX MEDLINE=20289799; PubMed=10830953; DOI=10.1038/35012518; RA Hattori M., Fujiyama A., Taylor T.D., Watanabe H., Yada T., RA Park H.-S., Toyoda A., Ishii K., Totoki Y., Choi D.-K., Groner Y., RA Soeda E., Ohki M., Takagi T., Sakaki Y., Taudien S., Blechschmidt K., RA Polley A., Menzel U., Delabar J., Kumpf K., Lehmann R., Patterson D., RA Reichwald K., Rump A., Schillhabel M., Schudy A., Zimmermann W., RA Rosenthal A., Kudoh J., Shibuya K., Kawasaki K., Asakawa S., RA Shintani A., Sasaki T., Nagamine K., Mitsuyama S., Antonarakis S.E., RA Minoshima S., Shimizu N., Nordsiek G., Hornischer K., Brandt P., RA Scharfe M., Schoen O., Desario A., Reichelt J., Kauer G., Bloecker H., RA Ramser J., Beck A., Klages S., Hennig S., Riesselmann L., Dagand E., RA Wehrmeyer S., Borzym K., Gardiner K., Nizetic D., Francis F., RA Lehrach H., Reinhardt R., Yaspo M.-L.; RT "The DNA sequence of human chromosome 21."; RL Nature 405:311-319(2000). RN [3] RP FUNCTION, AND INTERACTION WITH TRMT112. RX PubMed=18539146; DOI=10.1016/j.febslet.2008.05.045; RA Figaro S., Scrima N., Buckingham R.H., Heurgue-Hamard V.; RT "HemK2 protein, encoded on human chromosome 21, methylates translation RT termination factor eRF1."; RL FEBS Lett. 582:2352-2356(2008). RN [4] RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RA Colinge J., Superti-Furga G., Bennett K.L.; RL Submitted (OCT-2008) to UniProtKB. CC -!- FUNCTION: Heterodimeric methyltransferase that catalyzes N5- CC methylation of ETF1 on 'Gln-185', using S-adenosyl L-methionine as CC methyl donor. ETF1 needs to be complexed to ERF3 in its GTP-bound CC form to be efficiently methylated. CC -!- SUBUNIT: Heterodimer with TRMT112. CC -!- SIMILARITY: Belongs to the hemK family. CC -!- CAUTION: Was originally (Ref.1) proposed to be a DNA CC methyltransferase, but was then shown (PubMed:18539146) to be a CC protein methyltransferase. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AF139682; AAD38520.1; -; mRNA. DR EMBL; AL163248; CAB90428.1; -; Genomic_DNA. DR IPI; IPI00001640; -. DR UniGene; Hs.163846; -. DR MINT; MINT-3087317; -. DR STRING; Q9Y5N5; -. DR PRIDE; Q9Y5N5; -. DR Ensembl; ENST00000303775; ENSP00000303584; ENSG00000156239; Homo sapiens. DR UCSC; uc002ymo.1; human. DR GeneCards; GC21M029166; -. DR HGNC; HGNC:16021; N6AMT1. DR HOGENOM; HBG646581; -. DR HOVERGEN; HBG052569; -. DR InParanoid; Q9Y5N5; -. DR PhylomeDB; Q9Y5N5; -. DR ArrayExpress; Q9Y5N5; -. DR Bgee; Q9Y5N5; -. DR CleanEx; HS_N6AMT1; -. DR Genevestigator; Q9Y5N5; -. DR GermOnline; ENSG00000156239; Homo sapiens. DR GO; GO:0043234; C:protein complex; IDA:MGI. DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro. DR GO; GO:0005515; F:protein binding; IPI:UniProtKB. DR GO; GO:0008276; F:protein methyltransferase activity; IDA:MGI. DR GO; GO:0032259; P:methylation; IEA:InterPro. DR GO; GO:0030307; P:positive regulation of cell growth; IDA:MGI. DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS. DR InterPro; IPR004557; HemK_rel_arch. DR InterPro; IPR007848; Small_mtfrase. DR Pfam; PF05175; MTS; 1. DR TIGRFAMs; TIGR00537; hemK_rel_arch; 1. DR PROSITE; PS00092; N6_MTASE; 1. PE 1: Evidence at protein level; KW Complete proteome; Methyltransferase; Polymorphism; KW S-adenosyl-L-methionine; Transferase. FT CHAIN 1 214 HemK methyltransferase family member 2. FT /FTId=PRO_0000088049. FT REGION 53 57 S-adenosyl-L-methionine binding (By FT similarity). FT REGION 122 125 Substrate binding (By similarity). FT BINDING 77 77 S-adenosyl-L-methionine (By similarity). FT BINDING 122 122 S-adenosyl-L-methionine (By similarity). FT VARIANT 34 34 D -> N (in dbSNP:rs1997607). FT /FTId=VAR_060445. FT VARIANT 146 146 K -> R (in dbSNP:rs2205447). FT /FTId=VAR_060446. FT VARIANT 166 166 K -> R (in dbSNP:rs2205446). FT /FTId=VAR_060447. SQ SEQUENCE 214 AA; 22901 MW; 44F19A593BA29DCC CRC64; MAGENFATPF HGHVGRGAFS DVYEPAEDTF LLLDALEAAA AELAGVEICL EVGSGSGVVS AFLASMIGPQ ALYMCTDINP EAAACTLETA RCNKVHIQPV ITDLVKGLLP RLTEKVDLLV FNPPYVVTPP QEVGSHGIEA AWAGGKNGRE VMDRFFPLVP DLLSPKGLFY LVTIKENNPE EILKIMKTKG LQGTTALSRQ AGQETLSVLK FTKS //