ID NOP16_HUMAN Reviewed; 178 AA. AC Q9Y3C1; B4DV13; D6RGD3; Q05D05; Q6IAI6; Q6PIM0; Q8IXL5; DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot. DT 19-SEP-2006, sequence version 2. DT 16-JAN-2019, entry version 148. DE RecName: Full=Nucleolar protein 16; DE AltName: Full=HBV pre-S2 trans-regulated protein 3; GN Name=NOP16; ORFNames=CGI-117, HSPC111; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; OC Catarrhini; Hominidae; Homo. OX NCBI_TaxID=9606; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). RA Ji D., Cheng J., Dong J., Liu Y., Wang J.-J., Guo J.; RT "Screening and identification of genes trans-regulated by hepatitis B RT virus pre-S2 protein by microarray assay."; RL Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases. RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). RX PubMed=10810093; DOI=10.1101/gr.10.5.703; RA Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C.; RT "Identification of novel human genes evolutionarily conserved in RT Caenorhabditis elegans by comparative proteomics."; RL Genome Res. 10:703-713(2000). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). RC TISSUE=Umbilical cord blood; RX PubMed=11042152; DOI=10.1101/gr.140200; RA Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., RA Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., RA Tao J., Huang Q.-H., Zhou J., Hu G.-X., Gu J., Chen S.-J., Chen Z.; RT "Cloning and functional analysis of cDNAs with open reading frames for RT 300 previously undefined genes expressed in CD34+ hematopoietic RT stem/progenitor cells."; RL Genome Res. 10:1546-1560(2000). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). RC TISSUE=Small intestine; RX PubMed=14702039; DOI=10.1038/ng1285; RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S., RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.; RT "Complete sequencing and characterization of 21,243 full-length human RT cDNAs."; RL Nat. Genet. 36:40-45(2004). RN [5] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.; RT "Cloning of human full open reading frames in Gateway(TM) system entry RT vector (pDONR201)."; RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases. RN [6] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RX PubMed=15372022; DOI=10.1038/nature02919; RA Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., RA Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., RA She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S., RA Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., RA Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., RA Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., RA Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., RA Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., RA Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., RA Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., RA Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., RA Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., RA Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., RA Richardson P., Lucas S.M., Myers R.M., Rubin E.M.; RT "The DNA sequence and comparative analysis of human chromosome 5."; RL Nature 431:268-274(2004). RN [7] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RA Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R., RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., RA Venter J.C.; RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases. RN [8] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3). RC TISSUE=Lung, Mammary gland, and Skin; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA RT project: the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [9] RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. RC TISSUE=Cervix carcinoma; RX PubMed=12429849; DOI=10.1091/mbc.E02-05-0271; RA Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C., RA Greco A., Hochstrasser D.F., Diaz J.-J.; RT "Functional proteomic analysis of human nucleolus."; RL Mol. Biol. Cell 13:4100-4109(2002). RN [10] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16 AND THR-144, AND RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RC TISSUE=Cervix carcinoma; RX PubMed=18669648; DOI=10.1073/pnas.0805139105; RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., RA Elledge S.J., Gygi S.P.; RT "A quantitative atlas of mitotic phosphorylation."; RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). RN [11] RP ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-90, AND IDENTIFICATION BY RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RX PubMed=19608861; DOI=10.1126/science.1175371; RA Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., RA Walther T.C., Olsen J.V., Mann M.; RT "Lysine acetylation targets protein complexes and co-regulates major RT cellular functions."; RL Science 325:834-840(2009). RN [12] RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RX PubMed=21269460; DOI=10.1186/1752-0509-5-17; RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.; RT "Initial characterization of the human central proteome."; RL BMC Syst. Biol. 5:17-17(2011). RN [13] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-8, AND IDENTIFICATION BY RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RX PubMed=21406692; DOI=10.1126/scisignal.2001570; RA Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., RA Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., RA Blagoev B.; RT "System-wide temporal characterization of the proteome and RT phosphoproteome of human embryonic stem cell differentiation."; RL Sci. Signal. 4:RS3-RS3(2011). RN [14] RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RX PubMed=22814378; DOI=10.1073/pnas.1210303109; RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., RA Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., RA Aldabe R.; RT "N-terminal acetylome analyses and functional insights of the N- RT terminal acetyltransferase NatB."; RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012). RN [15] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16, AND IDENTIFICATION RP BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RC TISSUE=Cervix carcinoma, and Erythroleukemia; RX PubMed=23186163; DOI=10.1021/pr300630k; RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J., RA Mohammed S.; RT "Toward a comprehensive characterization of a human cancer cell RT phosphoproteome."; RL J. Proteome Res. 12:260-271(2013). RN [16] RP SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-74, SUMOYLATION [LARGE SCALE RP ANALYSIS] AT LYS-166 AND LYS-172 (ISOFORM 2), SUMOYLATION [LARGE SCALE RP ANALYSIS] AT LYS-167 AND LYS-173 (ISOFORM 3), AND IDENTIFICATION BY RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RX PubMed=28112733; DOI=10.1038/nsmb.3366; RA Hendriks I.A., Lyon D., Young C., Jensen L.J., Vertegaal A.C., RA Nielsen M.L.; RT "Site-specific mapping of the human SUMO proteome reveals co- RT modification with phosphorylation."; RL Nat. Struct. Mol. Biol. 24:325-336(2017). CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus CC {ECO:0000269|PubMed:12429849}. CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=3; CC Name=1; CC IsoId=Q9Y3C1-1; Sequence=Displayed; CC Name=2; CC IsoId=Q9Y3C1-2; Sequence=VSP_045583; CC Note=Ref.4 (BAG62525) sequence is in conflict in position: CC 171:C->F. Contains a glycyl lysine isopeptide (Lys-Gly) CC (interchain with G-Cter in SUMO2) at position 166. Contains a CC glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in CC SUMO2) at position 172. {ECO:0000244|PubMed:28112733, CC ECO:0000305}; CC Name=3; CC IsoId=Q9Y3C1-3; Sequence=VSP_057393; CC Note=No experimental confirmation available. Contains a glycyl CC lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) at CC position 167. Contains a glycyl lysine isopeptide (Lys-Gly) CC (interchain with G-Cter in SUMO2) at position 173. CC {ECO:0000244|PubMed:28112733}; CC -!- SIMILARITY: Belongs to the NOP16 family. {ECO:0000305}. CC -!- SEQUENCE CAUTION: CC Sequence=AAH19331.1; Type=Frameshift; Positions=195; Evidence={ECO:0000305}; CC Sequence=BAG62525.1; Type=Frameshift; Positions=195; Evidence={ECO:0000305}; CC Sequence=EAW85085.1; Type=Frameshift; Positions=195; Evidence={ECO:0000305}; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AY561704; AAS66977.1; -; mRNA. DR EMBL; AF151875; AAD34112.1; -; mRNA. DR EMBL; AF161460; AAF29075.1; -; mRNA. DR EMBL; AK300883; BAG62525.1; ALT_FRAME; mRNA. DR EMBL; CR457169; CAG33450.1; -; mRNA. DR EMBL; AC138956; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR EMBL; KF459629; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR EMBL; CH471195; EAW85084.1; -; Genomic_DNA. DR EMBL; CH471195; EAW85085.1; ALT_FRAME; Genomic_DNA. DR EMBL; BC019331; AAH19331.1; ALT_FRAME; mRNA. DR EMBL; BC032424; AAH32424.1; -; mRNA. DR EMBL; BC040106; AAH40106.1; -; mRNA. DR CCDS; CCDS43403.1; -. [Q9Y3C1-1] DR CCDS; CCDS78091.1; -. [Q9Y3C1-3] DR RefSeq; NP_001243468.2; NM_001256539.3. [Q9Y3C1-3] DR RefSeq; NP_001243469.2; NM_001256540.3. DR RefSeq; NP_001278234.1; NM_001291305.2. DR RefSeq; NP_001278236.1; NM_001291307.2. DR RefSeq; NP_001278237.1; NM_001291308.2. DR RefSeq; NP_001304904.1; NM_001317975.1. DR RefSeq; NP_057475.2; NM_016391.7. [Q9Y3C1-1] DR UniGene; Hs.696283; -. DR ProteinModelPortal; Q9Y3C1; -. DR BioGrid; 119568; 44. DR IntAct; Q9Y3C1; 16. DR STRING; 9606.ENSP00000373810; -. DR iPTMnet; Q9Y3C1; -. DR PhosphoSitePlus; Q9Y3C1; -. DR SwissPalm; Q9Y3C1; -. DR BioMuta; NOP16; -. DR DMDM; 115311631; -. DR SWISS-2DPAGE; Q9Y3C1; -. DR EPD; Q9Y3C1; -. DR jPOST; Q9Y3C1; -. DR PaxDb; Q9Y3C1; -. DR PeptideAtlas; Q9Y3C1; -. DR PRIDE; Q9Y3C1; -. DR ProteomicsDB; 86010; -. DR TopDownProteomics; Q9Y3C1-1; -. [Q9Y3C1-1] DR DNASU; 51491; -. DR Ensembl; ENST00000614830; ENSP00000480832; ENSG00000048162. [Q9Y3C1-1] DR Ensembl; ENST00000618911; ENSP00000483001; ENSG00000048162. [Q9Y3C1-3] DR GeneID; 51491; -. DR KEGG; hsa:51491; -. DR UCSC; uc032vuv.2; human. [Q9Y3C1-1] DR UCSC; uc063jyi.1; human. DR CTD; 51491; -. DR DisGeNET; 51491; -. DR EuPathDB; HostDB:ENSG00000048162.19; -. DR GeneCards; NOP16; -. DR HGNC; HGNC:26934; NOP16. DR HPA; HPA036506; -. DR HPA; HPA058147; -. DR MIM; 612861; gene. DR neXtProt; NX_Q9Y3C1; -. DR OpenTargets; ENSG00000048162; -. DR PharmGKB; PA164724025; -. DR eggNOG; KOG4706; Eukaryota. DR eggNOG; ENOG4111QB6; LUCA. DR GeneTree; ENSGT00940000163830; -. DR HOGENOM; HOG000169215; -. DR HOVERGEN; HBG098588; -. DR InParanoid; Q9Y3C1; -. DR OMA; TYLMDKY; -. DR OrthoDB; 1431164at2759; -. DR PhylomeDB; Q9Y3C1; -. DR TreeFam; TF323541; -. DR ChiTaRS; NOP16; human. DR GenomeRNAi; 51491; -. DR PRO; PR:Q9Y3C1; -. DR Proteomes; UP000005640; Chromosome 5. DR Bgee; ENSG00000048162; Expressed in 224 organ(s), highest expression level in layer of synovial tissue. DR CleanEx; HS_NOP16; -. DR ExpressionAtlas; Q9Y3C1; baseline and differential. DR Genevisible; Q9Y3C1; HS. DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IDA:HPA. DR GO; GO:0005730; C:nucleolus; IDA:HPA. DR GO; GO:0005634; C:nucleus; IDA:HPA. DR GO; GO:0003723; F:RNA binding; HDA:UniProtKB. DR GO; GO:0042273; P:ribosomal large subunit biogenesis; IBA:GO_Central. DR InterPro; IPR019002; Ribosome_biogenesis_Nop16. DR PANTHER; PTHR13243; PTHR13243; 1. DR Pfam; PF09420; Nop16; 2. PE 1: Evidence at protein level; KW Acetylation; Alternative splicing; Complete proteome; Isopeptide bond; KW Nucleus; Phosphoprotein; Reference proteome; Ubl conjugation. FT CHAIN 1 178 Nucleolar protein 16. FT /FTId=PRO_0000050817. FT MOD_RES 8 8 Phosphothreonine. FT {ECO:0000244|PubMed:21406692}. FT MOD_RES 16 16 Phosphoserine. FT {ECO:0000244|PubMed:18669648, FT ECO:0000244|PubMed:23186163}. FT MOD_RES 90 90 N6-acetyllysine. FT {ECO:0000244|PubMed:19608861}. FT MOD_RES 144 144 Phosphothreonine. FT {ECO:0000244|PubMed:18669648}. FT CROSSLNK 74 74 Glycyl lysine isopeptide (Lys-Gly) FT (interchain with G-Cter in SUMO2). FT {ECO:0000244|PubMed:28112733}. FT VAR_SEQ 132 178 AMARDEKNYYQDTPKQIRSKINVYKRFYPAEWQDFLDSLQK FT RKMEVE -> SGKTSSILCRRGRWRWSDWFTSQLPQAEASP FT GPVKLEPGCKARRCCVAPEELARSHGIRRLHTRAHSPLWGR FT NCSQRLQFIFIWGFTEKPEPAVFRVGDVNIVCT (in FT isoform 2). {ECO:0000303|PubMed:14702039, FT ECO:0000303|PubMed:15489334}. FT /FTId=VSP_045583. FT VAR_SEQ 132 178 AMARDEKNYYQDTPKQIRSKINVYKRFYPAEWQDFLDSLQK FT RKMEVE -> QSGKTSSILCRRGRWRWSDWFTSQLPQAEAS FT PGPVKLEPGCKARRCCVAPEELARSHGIRRLHTHVHTPRSG FT EGTVLRGSNLYSSGGSRKSQNLLFSGWVM (in isoform FT 3). {ECO:0000303|PubMed:15489334}. FT /FTId=VSP_057393. FT CONFLICT 32 33 PR -> RG (in Ref. 2; AAD34112 and 3; FT AAF29075). {ECO:0000305}. FT CONFLICT 178 178 E -> D (in Ref. 5; CAG33450). FT {ECO:0000305}. SQ SEQUENCE 178 AA; 21188 MW; D9139BE32DA81FC5 CRC64; MPKAKGKTRR QKFGYSVNRK RLNRNARRKA APRIECSHIR HAWDHAKSVR QNLAEMGLAV DPNRAVPLRK RKVKAMEVDI EERPKELVRK PYVLNDLEAE ASLPEKKGNT LSRDLIDYVR YMVENHGEDY KAMARDEKNY YQDTPKQIRS KINVYKRFYP AEWQDFLDSL QKRKMEVE //