ID S35A3_HUMAN Reviewed; 325 AA. AC Q9Y2D2; A8K3F8; D3DT54; Q68CR2; Q9BSB7; DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot. DT 01-NOV-1999, sequence version 1. DT 16-APR-2014, entry version 113. DE RecName: Full=UDP-N-acetylglucosamine transporter; DE AltName: Full=Golgi UDP-GlcNAc transporter; DE AltName: Full=Solute carrier family 35 member A3; GN Name=SLC35A3; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; OC Catarrhini; Hominidae; Homo. OX NCBI_TaxID=9606; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). RX PubMed=10393322; RA Ishida N., Yoshioka S., Chiba Y., Takeuchi M., Kawakita M.; RT "Molecular cloning and functional expression of the human Golgi UDP-N- RT acetylglucosamine transporter."; RL J. Biochem. 126:68-77(1999). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). RX PubMed=14702039; DOI=10.1038/ng1285; RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S., RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.; RT "Complete sequencing and characterization of 21,243 full-length human RT cDNAs."; RL Nat. Genet. 36:40-45(2004). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). RC TISSUE=Colon carcinoma; RX PubMed=17974005; DOI=10.1186/1471-2164-8-399; RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., RA Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., RA Ottenwaelder B., Poustka A., Wiemann S., Schupp I.; RT "The full-ORF clone resource of the German cDNA consortium."; RL BMC Genomics 8:399-399(2007). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RX PubMed=16710414; DOI=10.1038/nature04727; RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., RA Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., RA Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., RA McDonald L., Evans R., Phillips K., Atkinson A., Cooper R., Jones C., RA Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., RA Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., RA Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., RA Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., RA Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., RA Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., RA Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., RA Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., RA Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., RA Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., RA Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., RA Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., RA Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., RA Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., RA Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., RA Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., RA Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., RA Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., RA Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., RA Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., RA Beck S., Rogers J., Bentley D.R.; RT "The DNA sequence and biological annotation of human chromosome 1."; RL Nature 441:315-321(2006). RN [5] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R., RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., RA Venter J.C.; RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases. RN [6] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). RC TISSUE=Placenta; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA RT project: the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). CC -!- FUNCTION: Uridine diphosphate-N-acetylglucosamine transporter in CC the Golgi apparatus. CC -!- INTERACTION: CC P78381-1:SLC35A2; NbExp=3; IntAct=EBI-3917581, EBI-8101118; CC P78381-2:SLC35A2; NbExp=2; IntAct=EBI-3917581, EBI-8101093; CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane; Multi-pass CC membrane protein. CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=3; CC Name=1; CC IsoId=Q9Y2D2-1; Sequence=Displayed; CC Name=2; CC IsoId=Q9Y2D2-2; Sequence=VSP_012786; CC Note=No experimental confirmation available; CC Name=3; CC IsoId=Q9Y2D2-3; Sequence=VSP_054232, VSP_054233; CC Note=No experimental confirmation available; CC -!- SIMILARITY: Belongs to the nucleotide-sugar transporter family. CC SLC35A subfamily. CC -!- WEB RESOURCE: Name=GGDB; Note=GlycoGene database; CC URL="http://jcggdb.jp/rcmg/ggdb/Homolog?cat=symbol&symbol=SLC35A3"; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AB021981; BAA77841.1; -; mRNA. DR EMBL; AK290573; BAF83262.1; -; mRNA. DR EMBL; CR749816; CAH18676.1; -; mRNA. DR EMBL; AC118553; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR EMBL; CH471097; EAW72977.1; -; Genomic_DNA. DR EMBL; CH471097; EAW72978.1; -; Genomic_DNA. DR EMBL; CH471097; EAW72979.1; -; Genomic_DNA. DR EMBL; BC005136; AAH05136.1; -; mRNA. DR RefSeq; NP_001258613.1; NM_001271684.1. DR RefSeq; NP_001258614.1; NM_001271685.1. DR RefSeq; NP_036375.1; NM_012243.2. DR RefSeq; XP_005270748.1; XM_005270691.2. DR UniGene; Hs.448979; -. DR UniGene; Hs.534953; -. DR ProteinModelPortal; Q9Y2D2; -. DR BioGrid; 117010; 3. DR IntAct; Q9Y2D2; 3. DR MINT; MINT-4781806; -. DR STRING; 9606.ENSP00000359174; -. DR TCDB; 2.A.7.12.7; the drug/metabolite transporter (dmt) superfamily. DR PhosphoSite; Q9Y2D2; -. DR DMDM; 9087207; -. DR PaxDb; Q9Y2D2; -. DR PRIDE; Q9Y2D2; -. DR Ensembl; ENST00000370153; ENSP00000359172; ENSG00000117620. DR Ensembl; ENST00000370155; ENSP00000359174; ENSG00000117620. [Q9Y2D2-1] DR Ensembl; ENST00000427993; ENSP00000414947; ENSG00000117620. [Q9Y2D2-1] DR Ensembl; ENST00000465289; ENSP00000418527; ENSG00000117620. DR Ensembl; ENST00000533028; ENSP00000433849; ENSG00000117620. [Q9Y2D2-1] DR GeneID; 23443; -. DR KEGG; hsa:23443; -. DR UCSC; uc001dsp.2; human. [Q9Y2D2-1] DR UCSC; uc001dsr.2; human. [Q9Y2D2-2] DR CTD; 23443; -. DR GeneCards; GC01P100435; -. DR HGNC; HGNC:11023; SLC35A3. DR HPA; HPA015253; -. DR MIM; 605632; gene. DR neXtProt; NX_Q9Y2D2; -. DR Orphanet; 370943; Autism spectrum disorder-epilepsy-arthrogryposis syndrome. DR PharmGKB; PA35891; -. DR eggNOG; COG0697; -. DR HOGENOM; HOG000216649; -. DR HOVERGEN; HBG054025; -. DR InParanoid; Q9Y2D2; -. DR KO; K15272; -. DR OrthoDB; EOG7NCV4D; -. DR PhylomeDB; Q9Y2D2; -. DR TreeFam; TF315345; -. DR Reactome; REACT_15518; Transmembrane transport of small molecules. DR ChiTaRS; SLC35A3; human. DR GenomeRNAi; 23443; -. DR NextBio; 45719; -. DR PRO; PR:Q9Y2D2; -. DR ArrayExpress; Q9Y2D2; -. DR Bgee; Q9Y2D2; -. DR CleanEx; HS_SLC35A3; -. DR Genevestigator; Q9Y2D2; -. DR GO; GO:0005794; C:Golgi apparatus; TAS:ProtInc. DR GO; GO:0000139; C:Golgi membrane; TAS:Reactome. DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW. DR GO; GO:0005351; F:sugar:hydrogen symporter activity; IEA:InterPro. DR GO; GO:0005462; F:UDP-N-acetylglucosamine transmembrane transporter activity; TAS:ProtInc. DR GO; GO:0055085; P:transmembrane transport; TAS:Reactome. DR GO; GO:0006047; P:UDP-N-acetylglucosamine metabolic process; TAS:ProtInc. DR GO; GO:0015788; P:UDP-N-acetylglucosamine transport; TAS:ProtInc. DR InterPro; IPR007271; Nuc_sug_transpt. DR InterPro; IPR021189; UDP/CMP-sugar_transptr. DR InterPro; IPR004689; UDPgal_transpt. DR PANTHER; PTHR10231; PTHR10231; 1. DR Pfam; PF04142; Nuc_sug_transp; 1. DR PIRSF; PIRSF005799; UDP-gal_transpt; 1. DR TIGRFAMs; TIGR00803; nst; 1. PE 1: Evidence at protein level; KW Alternative splicing; Complete proteome; Golgi apparatus; Membrane; KW Reference proteome; Sugar transport; Transmembrane; KW Transmembrane helix; Transport. FT CHAIN 1 325 UDP-N-acetylglucosamine transporter. FT /FTId=PRO_0000213357. FT TRANSMEM 8 24 Helical; (Potential). FT TRANSMEM 42 58 Helical; (Potential). FT TRANSMEM 138 154 Helical; (Potential). FT TRANSMEM 173 189 Helical; (Potential). FT TRANSMEM 209 225 Helical; (Potential). FT TRANSMEM 246 262 Helical; (Potential). FT TRANSMEM 268 284 Helical; (Potential). FT TRANSMEM 295 311 Helical; (Potential). FT VAR_SEQ 1 1 M -> MSSRSVLSPVVGTDAPDQHLELKKPQELKEMERLPL FT ANEDKT (in isoform 2). FT /FTId=VSP_012786. FT VAR_SEQ 212 220 GFFGSIFGL -> VSFSLEPSL (in isoform 3). FT /FTId=VSP_054232. FT VAR_SEQ 221 325 Missing (in isoform 3). FT /FTId=VSP_054233. SQ SEQUENCE 325 AA; 35985 MW; 69DFD944E37206C8 CRC64; MFANLKYVSL GILVFQTTSL VLTMRYSRTL KEEGPRYLSS TAVVVAELLK IMACILLVYK DSKCSLRALN RVLHDEILNK PMETLKLAIP SGIYTLQNNL LYVALSNLDA ATYQVTYQLK ILTTALFSVS MLSKKLGVYQ WLSLVILMTG VAFVQWPSDS QLDSKELSAG SQFVGLMAVL TACFSSGFAG VYFEKILKET KQSVWIRNIQ LGFFGSIFGL MGVYIYDGEL VSKNGFFQGY NRLTWIVVVL QALGGLVIAA VIKYADNILK GFATSLSIIL STLISYFWLQ DFVPTSVFFL GAILVITATF LYGYDPKPAG NPTKA //