ID T3JAM_HUMAN Reviewed; 551 AA. AC Q9Y228; A1L464; A6NIU9; Q2YDB5; Q4VY06; Q7Z706; DT 07-JUN-2004, integrated into UniProtKB/Swiss-Prot. DT 21-FEB-2006, sequence version 2. DT 22-APR-2020, entry version 142. DE RecName: Full=TRAF3-interacting JNK-activating modulator; DE AltName: Full=TRAF3-interacting protein 3; GN Name=TRAF3IP3; Synonyms=T3JAM; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; OC Homo. OX NCBI_TaxID=9606; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). RA Rhodes S.; RL Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases. RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORM 1). RX PubMed=16710414; DOI=10.1038/nature04727; RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., RA Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., RA Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K., RA Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., RA Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., RA Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., RA Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., RA Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., RA Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., RA Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., RA Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., RA Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., RA Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., RA Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., RA Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., RA Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., RA McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., RA Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., RA White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., RA Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., RA Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., RA Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.; RT "The DNA sequence and biological annotation of human chromosome 1."; RL Nature 441:315-321(2006). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANT GLU-373. RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., RA Hunkapiller M.W., Myers E.W., Venter J.C.; RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases. RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3), AND VARIANT RP GLU-373. RC TISSUE=Blood, and Brain; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA project: RT the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [5] RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RX PubMed=25944712; DOI=10.1002/pmic.201400617; RA Vaca Jacome A.S., Rabilloud T., Schaeffer-Reiss C., Rompais M., Ayoub D., RA Lane L., Bairoch A., Van Dorsselaer A., Carapito C.; RT "N-terminome analysis of the human mitochondrial proteome."; RL Proteomics 15:2519-2524(2015). RN [6] RP VARIANT [LARGE SCALE ANALYSIS] SER-529. RX PubMed=16959974; DOI=10.1126/science.1133427; RA Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., RA Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., RA Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V., RA Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., RA Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., RA Velculescu V.E.; RT "The consensus coding sequences of human breast and colorectal cancers."; RL Science 314:268-274(2006). CC -!- FUNCTION: May function as an adapter molecule that regulates TRAF3- CC mediated JNK activation. {ECO:0000250}. CC -!- SUBUNIT: Binds to the isoleucine zipper of TRAF3 via its coiled-coil CC domain. {ECO:0000250}. CC -!- INTERACTION: CC Q9Y228; Q8N350-4: CBARP; NbExp=3; IntAct=EBI-765817, EBI-19051169; CC Q9Y228; Q96M89-2: CCDC138; NbExp=3; IntAct=EBI-765817, EBI-10972887; CC Q9Y228; Q8N6L0: CCDC155; NbExp=6; IntAct=EBI-765817, EBI-749265; CC Q9Y228; A6NC98: CCDC88B; NbExp=3; IntAct=EBI-765817, EBI-347573; CC Q9Y228; P24863: CCNC; NbExp=3; IntAct=EBI-765817, EBI-395261; CC Q9Y228; Q96GN5: CDCA7L; NbExp=3; IntAct=EBI-765817, EBI-5278764; CC Q9Y228; Q76N32-2: CEP68; NbExp=3; IntAct=EBI-765817, EBI-11975967; CC Q9Y228; Q8NHQ1: CEP70; NbExp=3; IntAct=EBI-765817, EBI-739624; CC Q9Y228; O75208: COQ9; NbExp=3; IntAct=EBI-765817, EBI-724524; CC Q9Y228; Q7Z7G2: CPLX4; NbExp=3; IntAct=EBI-765817, EBI-18013275; CC Q9Y228; Q68CJ9: CREB3L3; NbExp=3; IntAct=EBI-765817, EBI-852194; CC Q9Y228; O00559: EBAG9; NbExp=3; IntAct=EBI-765817, EBI-8787095; CC Q9Y228; P50402: EMD; NbExp=9; IntAct=EBI-765817, EBI-489887; CC Q9Y228; P34910-2: EVI2B; NbExp=3; IntAct=EBI-765817, EBI-17640610; CC Q9Y228; Q5JX71: FAM209A; NbExp=3; IntAct=EBI-765817, EBI-18304435; CC Q9Y228; Q969F0: FATE1; NbExp=6; IntAct=EBI-765817, EBI-743099; CC Q9Y228; A6NEM1: GOLGA6L9; NbExp=3; IntAct=EBI-765817, EBI-5916454; CC Q9Y228; Q5T7V8: GORAB; NbExp=3; IntAct=EBI-765817, EBI-3917143; CC Q9Y228; Q8IUY3: GRAMD2A; NbExp=3; IntAct=EBI-765817, EBI-11984319; CC Q9Y228; P43628: KIR2DL3; NbExp=3; IntAct=EBI-765817, EBI-8632435; CC Q9Y228; Q7Z3Y8: KRT27; NbExp=3; IntAct=EBI-765817, EBI-3044087; CC Q9Y228; Q96JM7-2: L3MBTL3; NbExp=5; IntAct=EBI-765817, EBI-11985629; CC Q9Y228; Q9H400: LIME1; NbExp=3; IntAct=EBI-765817, EBI-2830566; CC Q9Y228; Q8N386: LRRC25; NbExp=3; IntAct=EBI-765817, EBI-11304917; CC Q9Y228; Q9GZY8-5: MFF; NbExp=3; IntAct=EBI-765817, EBI-11956541; CC Q9Y228; Q9UPX6: MINAR1; NbExp=3; IntAct=EBI-765817, EBI-11977115; CC Q9Y228; Q9Y3A3: MOB4; NbExp=2; IntAct=EBI-765817, EBI-713935; CC Q9Y228; Q9GZM8: NDEL1; NbExp=6; IntAct=EBI-765817, EBI-928842; CC Q9Y228; Q9H4L5: OSBPL3; NbExp=3; IntAct=EBI-765817, EBI-1051317; CC Q9Y228; Q53GL0: PLEKHO1; NbExp=3; IntAct=EBI-765817, EBI-949945; CC Q9Y228; O43586: PSTPIP1; NbExp=3; IntAct=EBI-765817, EBI-1050964; CC Q9Y228; Q9H0H5: RACGAP1; NbExp=3; IntAct=EBI-765817, EBI-717233; CC Q9Y228; Q7Z5B4-5: RIC3; NbExp=3; IntAct=EBI-765817, EBI-12375429; CC Q9Y228; Q5EBL4-3: RILPL1; NbExp=3; IntAct=EBI-765817, EBI-12072024; CC Q9Y228; Q9BRV8: SIKE1; NbExp=2; IntAct=EBI-765817, EBI-1773646; CC Q9Y228; P23497: SP100; NbExp=4; IntAct=EBI-765817, EBI-751145; CC Q9Y228; P23497-2: SP100; NbExp=3; IntAct=EBI-765817, EBI-6589365; CC Q9Y228; Q9Y6E0: STK24; NbExp=2; IntAct=EBI-765817, EBI-740175; CC Q9Y228; Q9H169-2: STMN4; NbExp=3; IntAct=EBI-765817, EBI-20117546; CC Q9Y228; Q5VSL9: STRIP1; NbExp=2; IntAct=EBI-765817, EBI-1773588; CC Q9Y228; O43815: STRN; NbExp=2; IntAct=EBI-765817, EBI-1046642; CC Q9Y228; Q13033: STRN3; NbExp=2; IntAct=EBI-765817, EBI-1053857; CC Q9Y228; Q16623: STX1A; NbExp=3; IntAct=EBI-765817, EBI-712466; CC Q9Y228; Q12846: STX4; NbExp=3; IntAct=EBI-765817, EBI-744942; CC Q9Y228; Q9UBB9: TFIP11; NbExp=4; IntAct=EBI-765817, EBI-1105213; CC Q9Y228; Q8IV31: TMEM139; NbExp=3; IntAct=EBI-765817, EBI-7238458; CC Q9Y228; Q9NQ86: TRIM36; NbExp=3; IntAct=EBI-765817, EBI-2341518; CC Q9Y228; Q9BTA9: WAC; NbExp=3; IntAct=EBI-765817, EBI-749118; CC Q9Y228; O60232: ZNRD2; NbExp=3; IntAct=EBI-765817, EBI-741415; CC Q9Y228; Q9UGI0: ZRANB1; NbExp=3; IntAct=EBI-765817, EBI-527853; CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type IV CC membrane protein {ECO:0000305}. CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=3; CC Name=1; CC IsoId=Q9Y228-1; Sequence=Displayed; CC Name=2; CC IsoId=Q9Y228-2; Sequence=VSP_017271; CC Name=3; CC IsoId=Q9Y228-3; Sequence=VSP_017272, VSP_017273; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AL049667; CAB41242.1; -; mRNA. DR EMBL; AL022398; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR EMBL; CH471100; EAW93444.1; -; Genomic_DNA. DR EMBL; BC110302; AAI10303.1; -; mRNA. DR EMBL; BC130417; AAI30418.1; -; mRNA. DR EMBL; BC144139; AAI44140.1; -; mRNA. DR CCDS; CCDS1490.2; -. [Q9Y228-1] DR CCDS; CCDS81422.1; -. [Q9Y228-2] DR RefSeq; NP_001274683.1; NM_001287754.1. DR RefSeq; NP_001307072.1; NM_001320143.1. [Q9Y228-1] DR RefSeq; NP_001307073.1; NM_001320144.1. [Q9Y228-2] DR RefSeq; NP_079504.2; NM_025228.3. [Q9Y228-1] DR SMR; Q9Y228; -. DR BioGrid; 123249; 36. DR IntAct; Q9Y228; 81. DR MINT; Q9Y228; -. DR STRING; 9606.ENSP00000355991; -. DR iPTMnet; Q9Y228; -. DR MetOSite; Q9Y228; -. DR PhosphoSitePlus; Q9Y228; -. DR SwissPalm; Q9Y228; -. DR BioMuta; TRAF3IP3; -. DR DMDM; 88984949; -. DR EPD; Q9Y228; -. DR jPOST; Q9Y228; -. DR MassIVE; Q9Y228; -. DR PaxDb; Q9Y228; -. DR PeptideAtlas; Q9Y228; -. DR PRIDE; Q9Y228; -. DR ProteomicsDB; 85616; -. [Q9Y228-1] DR ProteomicsDB; 85617; -. [Q9Y228-2] DR ProteomicsDB; 85618; -. [Q9Y228-3] DR Antibodypedia; 34596; 188 antibodies. DR DNASU; 80342; -. DR Ensembl; ENST00000367024; ENSP00000355991; ENSG00000009790. [Q9Y228-1] DR Ensembl; ENST00000367025; ENSP00000355992; ENSG00000009790. [Q9Y228-1] DR Ensembl; ENST00000367026; ENSP00000355993; ENSG00000009790. [Q9Y228-2] DR Ensembl; ENST00000478359; ENSP00000417665; ENSG00000009790. [Q9Y228-3] DR GeneID; 80342; -. DR KEGG; hsa:80342; -. DR UCSC; uc001hhm.4; human. [Q9Y228-1] DR CTD; 80342; -. DR DisGeNET; 80342; -. DR GeneCards; TRAF3IP3; -. DR HGNC; HGNC:30766; TRAF3IP3. DR HPA; ENSG00000009790; Tissue enhanced (blood, lymphoid tissue). DR MIM; 608255; gene. DR neXtProt; NX_Q9Y228; -. DR OpenTargets; ENSG00000009790; -. DR PharmGKB; PA142670711; -. DR eggNOG; ENOG410IHX6; Eukaryota. DR eggNOG; ENOG410ZX9A; LUCA. DR GeneTree; ENSGT00940000160260; -. DR HOGENOM; CLU_038582_0_0_1; -. DR InParanoid; Q9Y228; -. DR OMA; QQQFFRR; -. DR OrthoDB; 484467at2759; -. DR PhylomeDB; Q9Y228; -. DR TreeFam; TF334641; -. DR ChiTaRS; TRAF3IP3; human. DR GeneWiki; TRAF3IP3; -. DR GenomeRNAi; 80342; -. DR Pharos; Q9Y228; Tbio. DR PRO; PR:Q9Y228; -. DR Proteomes; UP000005640; Chromosome 1. DR RNAct; Q9Y228; protein. DR Bgee; ENSG00000009790; Expressed in small intestine Peyer's patch and 151 other tissues. DR ExpressionAtlas; Q9Y228; baseline and differential. DR Genevisible; Q9Y228; HS. DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW. PE 1: Evidence at protein level; KW Alternative splicing; Coiled coil; Membrane; Polymorphism; KW Reference proteome; Transmembrane; Transmembrane helix. FT CHAIN 1..551 FT /note="TRAF3-interacting JNK-activating modulator" FT /id="PRO_0000072403" FT TOPO_DOM 1..526 FT /note="Cytoplasmic" FT /evidence="ECO:0000255" FT TRANSMEM 527..544 FT /note="Helical; Anchor for type IV membrane protein" FT /evidence="ECO:0000255" FT TOPO_DOM 545..551 FT /note="Extracellular" FT /evidence="ECO:0000255" FT COILED 240..436 FT /evidence="ECO:0000255" FT COILED 464..506 FT /evidence="ECO:0000255" FT VAR_SEQ 95..114 FT /note="Missing (in isoform 2)" FT /evidence="ECO:0000303|Ref.1" FT /id="VSP_017271" FT VAR_SEQ 352..353 FT /note="GA -> IN (in isoform 3)" FT /evidence="ECO:0000303|PubMed:15489334" FT /id="VSP_017272" FT VAR_SEQ 354..551 FT /note="Missing (in isoform 3)" FT /evidence="ECO:0000303|PubMed:15489334" FT /id="VSP_017273" FT VARIANT 373 FT /note="Q -> E (in dbSNP:rs669694)" FT /evidence="ECO:0000269|PubMed:15489334, ECO:0000269|Ref.3" FT /id="VAR_024283" FT VARIANT 529 FT /note="P -> S (in a colorectal cancer sample; somatic FT mutation)" FT /evidence="ECO:0000269|PubMed:16959974" FT /id="VAR_035664" SQ SEQUENCE 551 AA; 63626 MW; EA2DFF45A8D6ED19 CRC64; MISPDPRPSP GLARWAESYE AKCERRQEIR ESRRCRPNVT TCRQVGKTLR IQQREQLQRA RLQQFFRRRN LELEEKGKAQ HPQAREQGPS RRPGQVTVLK EPLSCARRIS SPREQVTGTS SEVFPAQHPP PSGICRDLSD HLSSQAGGLP PQDTPIKKPP KHHRGTQTKA EGPTIKNDAS QQTNYGVAVL DKEIIQLSDY LKEALQRELV LKQKMVILQD LLSTLIQASD SSWKGQLNED KLKGKLRSLE NQLYTCTQKY SPWGMKKVLL EMEDQKNSYE QKAKESLQKV LEEKMNAEQQ LQSTQRSLAL AEQKCEEWRS QYEALKEDWR TLGTQHRELE SQLHVLQSKL QGADSRDLQM NQALRFLENE HQQLQAKIEC LQGDRDLCSL DTQDLQDQLK RSEAEKLTLV TRVQQLQGLL QNQSLQLQEQ EKLLTKKDQA LPVWSPKSFP NEVEPEGTGK EKDWDLRDQL QKKTLQLQAK EKECRELHSE LDNLSDEYLS CLRKLQHCRE ELNQSQQLPP RRQCGRWLPV LMVVIAAALA VFLANKDNLM I //