ID NRA1_CAEEL Reviewed; 634 AA. AC Q9XUB9; Q8I4F4; DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot. DT 01-NOV-1999, sequence version 1. DT 07-NOV-2018, entry version 121. DE RecName: Full=Nicotinic receptor-associated protein 1; GN Name=nra-1; ORFNames=T28F3.1; OS Caenorhabditis elegans. OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; OC Caenorhabditis. OX NCBI_TaxID=6239; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE RP SPLICING. RC STRAIN=Bristol N2; RX PubMed=9851916; DOI=10.1126/science.282.5396.2012; RG The C. elegans sequencing consortium; RT "Genome sequence of the nematode C. elegans: a platform for RT investigating biology."; RL Science 282:2012-2018(1998). RN [2] RP FUNCTION, INTERACTION WITH NICOTINIC ACETYLCHOLINE RECEPTOR, RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE. RX PubMed=15990870; DOI=10.1038/sj.emboj.7600741; RA Gottschalk A., Almedom R.B., Schedletzky T., Anderson S.D., RA Yates J.R. III, Schafer W.R.; RT "Identification and characterization of novel nicotinic receptor- RT associated proteins in Caenorhabditis elegans."; RL EMBO J. 24:2566-2578(2005). RN [3] RP FUNCTION, AND DISRUPTION PHENOTYPE. RX PubMed=16998478; DOI=10.1038/ncb1476; RA Kubagawa H.M., Watts J.L., Corrigan C., Edmonds J.W., Sztul E., RA Browse J., Miller M.A.; RT "Oocyte signals derived from polyunsaturated fatty acids control sperm RT recruitment in vivo."; RL Nat. Cell Biol. 8:1143-1148(2006). CC -!- FUNCTION: Exhibits calcium-dependent phospholipid binding CC properties (By similarity). May function in membrane trafficking. CC Regulates synaptic levels of nicotinic acetylcholine receptor CC subunit lev-1 and unc-38 in the nerve cord (PubMed:15990870). CC Involved in nicotinic acetylcholine receptor (nAChR)-mediated CC sensitivity to nicotine and levamisole (PubMed:15990870). Affects CC directional sperm motility (PubMed:16998478). CC {ECO:0000250|UniProtKB:Q99829, ECO:0000269|PubMed:15990870, CC ECO:0000269|PubMed:16998478}. CC -!- SUBUNIT: Interacts with nicotinic acetylcholine receptor. CC {ECO:0000269|PubMed:16998478}. CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:15990870}; CC Peripheral membrane protein {ECO:0000269|PubMed:15990870}. CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=2; CC Name=a; CC IsoId=Q9XUB9-1; Sequence=Displayed; CC Note=No experimental confirmation available.; CC Name=b; CC IsoId=Q9XUB9-2; Sequence=VSP_039297; CC Note=No experimental confirmation available.; CC -!- TISSUE SPECIFICITY: Expressed in head and tail neurons, ventral CC cord moto-neurons, body wall muscles and hypodermal cells of the CC vulva. {ECO:0000269|PubMed:15990870}. CC -!- DOMAIN: C2 domains are necessary for calcium-dependent cell CC membrane association. C2 domains are necessary for neuronal CC progenitor cell differentiation in a calcium-independent manner. CC {ECO:0000250|UniProtKB:Q99829}. CC -!- DISRUPTION PHENOTYPE: Sperm directional motility defects CC (PubMed:16998478). RNAi-mediated knockdown causes a moderate CC resistance to nicotine-induced paralysis and a decrease in unc-38 CC synaptic expression along nerve cords without affecting lev-1 CC cellular expression levels in muscles (PubMed:15990870). CC {ECO:0000269|PubMed:15990870, ECO:0000269|PubMed:16998478}. CC -!- SIMILARITY: Belongs to the copine family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; Z82285; CAB05302.1; -; Genomic_DNA. DR EMBL; Z82285; CAD56248.1; -; Genomic_DNA. DR PIR; T25425; T25425. DR RefSeq; NP_503069.1; NM_070668.5. [Q9XUB9-1] DR RefSeq; NP_872104.1; NM_182304.3. [Q9XUB9-2] DR UniGene; Cel.17198; -. DR ProteinModelPortal; Q9XUB9; -. DR SMR; Q9XUB9; -. DR IntAct; Q9XUB9; 2. DR MINT; Q9XUB9; -. DR STRING; 6239.T28F3.1a.2; -. DR iPTMnet; Q9XUB9; -. DR EPD; Q9XUB9; -. DR PaxDb; Q9XUB9; -. DR PeptideAtlas; Q9XUB9; -. DR PRIDE; Q9XUB9; -. DR EnsemblMetazoa; T28F3.1a; T28F3.1a; WBGene00012128. [Q9XUB9-1] DR EnsemblMetazoa; T28F3.1b; T28F3.1b; WBGene00012128. [Q9XUB9-2] DR GeneID; 178508; -. DR KEGG; cel:CELE_T28F3.1; -. DR UCSC; T28F3.1a; c. elegans. DR CTD; 178508; -. DR WormBase; T28F3.1a; CE19806; WBGene00012128; nra-1. [Q9XUB9-1] DR WormBase; T28F3.1b; CE09683; WBGene00012128; nra-1. [Q9XUB9-2] DR eggNOG; KOG1327; Eukaryota. DR eggNOG; ENOG410XPC8; LUCA. DR GeneTree; ENSGT00760000119085; -. DR HOGENOM; HOG000220898; -. DR InParanoid; Q9XUB9; -. DR OMA; DMHATIR; -. DR OrthoDB; EOG091G05IT; -. DR PhylomeDB; Q9XUB9; -. DR PRO; PR:Q9XUB9; -. DR Proteomes; UP000001940; Chromosome IV. DR Bgee; WBGene00012128; Expressed in 5 organ(s), highest expression level in adult organism. DR GO; GO:0005886; C:plasma membrane; IDA:WormBase. DR GO; GO:0005544; F:calcium-dependent phospholipid binding; IBA:GO_Central. DR GO; GO:0071277; P:cellular response to calcium ion; IBA:GO_Central. DR GO; GO:0097120; P:receptor localization to synapse; IMP:WormBase. DR CDD; cd04047; C2B_Copine; 1. DR CDD; cd01459; vWA_copine_like; 1. DR Gene3D; 2.60.40.150; -; 2. DR InterPro; IPR000008; C2_dom. DR InterPro; IPR035892; C2_domain_sf. DR InterPro; IPR037768; C2B_Copine. DR InterPro; IPR010734; Copine. DR InterPro; IPR002035; VWF_A. DR InterPro; IPR036465; vWFA_dom_sf. DR Pfam; PF00168; C2; 2. DR Pfam; PF07002; Copine; 1. DR SMART; SM00239; C2; 2. DR SMART; SM00327; VWA; 1. DR SUPFAM; SSF53300; SSF53300; 1. DR PROSITE; PS50004; C2; 1. DR PROSITE; PS50234; VWFA; 1. PE 1: Evidence at protein level; KW Alternative splicing; Cell membrane; Complete proteome; Membrane; KW Reference proteome; Repeat. FT CHAIN 1 634 Nicotinic receptor-associated protein 1. FT /FTId=PRO_0000144851. FT DOMAIN 21 125 C2 1. {ECO:0000255|PROSITE- FT ProRule:PRU00041}. FT DOMAIN 182 197 C2 2. {ECO:0000255|PROSITE- FT ProRule:PRU00041}. FT DOMAIN 338 557 VWFA. {ECO:0000255|PROSITE- FT ProRule:PRU00219}. FT VAR_SEQ 258 269 Missing (in isoform b). {ECO:0000305}. FT /FTId=VSP_039297. SQ SEQUENCE 634 AA; 71101 MW; 9CF95130F9A2DCCE CRC64; MNQPIGIADS SRPKTNVRLT ISANNLMDLD VFSKSDPICL IYEKTSGRKA TTTEEITVPT WKDKQWTERG RTEVVMNNLN PQFTKTFLLP YFFEETQLLR FEIYDADSPT VGQDLSSHDF LGRFECVLAQ IVSYSTLKAH LGKTGQIGAQ WRNKDKNTKT GSITIYAEED EKAEKIQFDV CGEGLDKKDF FGKSDPYLNF KRKFDDGSTH LIHRTEVKPK TLDPRWATVQ INTQTLCAKD GDRPIIIECY DHDKWKKGEE PRGDAKFSRD DLIGTAQTTL NELLRGSSDA VEILLTNEKK KAKKGDKYKC SGTLKIWNSR IVIEPTFLDF ISGGTQLDFA VAVDFTASNG PPKSSSSLHF MSADRPNQYE LALRSVLSIC QHYNSSKTFE AFGFGAKLPN QSSVSAIFPL DLQRGTSEVV GITGVMTAYR HALSNVQLYG PTNFAPIIEN VARKAQNMIH DSARYQILLI ITDGIISDMH ATIRSIISAS GLPLSIIIIG VGNEDFEKMH ELDSDDALLQ QDSRIAQRDI VQFVTMREFL NNGQGLYLDP DVIQENLARE VLYEVPAQLT GYMKQRGFQP RPVDDPWRRD SPPPEFDPIL DGTGRRAPML QAPPAGFQYP VYADTSIASA PPMY //