ID TREA_DROME Reviewed; 596 AA. AC Q9W2M2; Q0E903; Q0E904; Q7YWL2; Q961P0; Q9W2M1; Q9W2M3; DT 03-JUL-2003, integrated into UniProtKB/Swiss-Prot. DT 01-MAY-2000, sequence version 1. DT 05-DEC-2018, entry version 133. DE RecName: Full=Trehalase {ECO:0000305}; DE EC=3.2.1.28 {ECO:0000250|UniProtKB:O43280}; DE AltName: Full=Alpha,alpha-trehalase; DE AltName: Full=Alpha,alpha-trehalose glucohydrolase; DE Flags: Precursor; GN Name=Treh; ORFNames=CG9364; OS Drosophila melanogaster (Fruit fly). OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; OC Pterygota; Neoptera; Holometabola; Diptera; Brachycera; Muscomorpha; OC Ephydroidea; Drosophilidae; Drosophila; Sophophora. OX NCBI_TaxID=7227; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley; RX PubMed=10731132; DOI=10.1126/science.287.5461.2185; RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., RA Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., RA Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., RA Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., RA Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., RA Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., RA Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., RA Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., RA Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., RA de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., RA Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., RA Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., RA Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., RA Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., RA Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., RA Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., RA Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., RA Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., RA Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., RA Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., RA Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., RA Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., RA Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., RA Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., RA Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., RA Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., RA Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., RA Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., RA Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., RA Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., RA Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.; RT "The genome sequence of Drosophila melanogaster."; RL Science 287:2185-2195(2000). RN [2] RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING. RC STRAIN=Berkeley; RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083; RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A., RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., RA Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., RA Lewis S.E.; RT "Annotation of the Drosophila melanogaster euchromatic genome: a RT systematic review."; RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). RC STRAIN=Berkeley; TISSUE=Head; RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080; RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., RA George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., RA Rubin G.M., Celniker S.E.; RT "A Drosophila full-length cDNA resource."; RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002). RN [4] RP GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-451, AND IDENTIFICATION BY RP MASS SPECTROMETRY. RC STRAIN=Oregon-R; TISSUE=Head; RX PubMed=17893096; DOI=10.1093/glycob/cwm097; RA Koles K., Lim J.-M., Aoki K., Porterfield M., Tiemeyer M., Wells L., RA Panin V.; RT "Identification of N-glycosylated proteins from the central nervous RT system of Drosophila melanogaster."; RL Glycobiology 17:1388-1403(2007). CC -!- CATALYTIC ACTIVITY: CC Reaction=alpha,alpha-trehalose + H2O = alpha-D-glucose + beta-D- CC glucose; Xref=Rhea:RHEA:32675, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:15903, ChEBI:CHEBI:16551, ChEBI:CHEBI:17925; CC EC=3.2.1.28; Evidence={ECO:0000250|UniProtKB:O43280}; CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=3; CC Name=C; Synonyms=F; CC IsoId=Q9W2M2-1; Sequence=Displayed; CC Note=No experimental confirmation available.; CC Name=A; Synonyms=D; CC IsoId=Q9W2M2-2; Sequence=VSP_007735; CC Note=No experimental confirmation available.; CC Name=B; CC IsoId=Q9W2M2-3; Sequence=VSP_021831; CC Note=No experimental confirmation available.; CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 37 family. CC {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AE013599; AAF46668.1; -; Genomic_DNA. DR EMBL; AE013599; AAF46669.1; -; Genomic_DNA. DR EMBL; AE013599; AAM68192.1; -; Genomic_DNA. DR EMBL; AY051466; AAK92890.1; -; mRNA. DR RefSeq; NP_001261115.1; NM_001274186.1. [Q9W2M2-2] DR RefSeq; NP_524821.1; NM_080082.3. [Q9W2M2-2] DR RefSeq; NP_726023.1; NM_166421.2. [Q9W2M2-2] DR RefSeq; NP_726024.1; NM_166422.2. [Q9W2M2-2] DR RefSeq; NP_726025.1; NM_166423.3. [Q9W2M2-1] DR RefSeq; NP_726026.1; NM_166424.2. [Q9W2M2-1] DR RefSeq; NP_726027.1; NM_166425.2. [Q9W2M2-3] DR UniGene; Dm.6686; -. DR ProteinModelPortal; Q9W2M2; -. DR SMR; Q9W2M2; -. DR BioGrid; 69627; 26. DR DIP; DIP-23202N; -. DR IntAct; Q9W2M2; 6. DR STRING; 7227.FBpp0071468; -. DR CAZy; GH37; Glycoside Hydrolase Family 37. DR iPTMnet; Q9W2M2; -. DR PaxDb; Q9W2M2; -. DR PRIDE; Q9W2M2; -. DR EnsemblMetazoa; FBtr0071535; FBpp0071464; FBgn0003748. [Q9W2M2-2] DR EnsemblMetazoa; FBtr0071536; FBpp0071465; FBgn0003748. [Q9W2M2-2] DR EnsemblMetazoa; FBtr0071537; FBpp0071466; FBgn0003748. [Q9W2M2-2] DR EnsemblMetazoa; FBtr0071538; FBpp0071467; FBgn0003748. [Q9W2M2-3] DR EnsemblMetazoa; FBtr0071539; FBpp0071468; FBgn0003748. [Q9W2M2-1] DR EnsemblMetazoa; FBtr0071540; FBpp0071469; FBgn0003748. [Q9W2M2-1] DR EnsemblMetazoa; FBtr0332423; FBpp0304696; FBgn0003748. [Q9W2M2-2] DR GeneID; 45368; -. DR KEGG; dme:Dmel_CG9364; -. DR UCSC; CG9364-RA; d. melanogaster. [Q9W2M2-1] DR CTD; 11181; -. DR FlyBase; FBgn0003748; Treh. DR eggNOG; KOG0602; Eukaryota. DR eggNOG; COG1626; LUCA. DR GeneTree; ENSGT00390000006949; -. DR InParanoid; Q9W2M2; -. DR KO; K01194; -. DR OMA; NRYWDAS; -. DR OrthoDB; EOG091G0341; -. DR PhylomeDB; Q9W2M2; -. DR GenomeRNAi; 45368; -. DR PRO; PR:Q9W2M2; -. DR Proteomes; UP000000803; Chromosome 2R. DR Bgee; FBgn0003748; Expressed in 43 organ(s), highest expression level in oviduct (Drosophila). DR ExpressionAtlas; Q9W2M2; baseline and differential. DR Genevisible; Q9W2M2; DM. DR GO; GO:0004555; F:alpha,alpha-trehalase activity; IDA:FlyBase. DR GO; GO:0097150; P:neuronal stem cell population maintenance; IMP:FlyBase. DR GO; GO:0005993; P:trehalose catabolic process; IMP:FlyBase. DR GO; GO:0005991; P:trehalose metabolic process; IMP:FlyBase. DR Gene3D; 1.50.10.10; -; 1. DR InterPro; IPR008928; 6-hairpin_glycosidase_sf. DR InterPro; IPR012341; 6hp_glycosidase-like_sf. DR InterPro; IPR001661; Glyco_hydro_37. DR InterPro; IPR018232; Glyco_hydro_37_CS. DR PANTHER; PTHR23403; PTHR23403; 1. DR Pfam; PF01204; Trehalase; 1. DR PRINTS; PR00744; GLHYDRLASE37. DR SUPFAM; SSF48208; SSF48208; 1. DR PROSITE; PS00927; TREHALASE_1; 1. DR PROSITE; PS00928; TREHALASE_2; 1. PE 1: Evidence at protein level; KW Alternative splicing; Complete proteome; Glycoprotein; Glycosidase; KW Hydrolase; Reference proteome; Signal. FT SIGNAL 1 23 {ECO:0000255}. FT CHAIN 24 596 Trehalase. FT /FTId=PRO_0000012058. FT REGION 200 201 Substrate binding. FT {ECO:0000250|UniProtKB:P13482}. FT REGION 246 248 Substrate binding. FT {ECO:0000250|UniProtKB:P13482}. FT REGION 307 309 Substrate binding. FT {ECO:0000250|UniProtKB:P13482}. FT ACT_SITE 343 343 Proton donor/acceptor. FT {ECO:0000250|UniProtKB:P13482}. FT ACT_SITE 541 541 Proton donor/acceptor. FT {ECO:0000250|UniProtKB:P13482}. FT BINDING 193 193 Substrate. FT {ECO:0000250|UniProtKB:P13482}. FT BINDING 237 237 Substrate. FT {ECO:0000250|UniProtKB:P13482}. FT BINDING 341 341 Substrate; via carbonyl oxygen. FT {ECO:0000250|UniProtKB:P13482}. FT BINDING 556 556 Substrate. FT {ECO:0000250|UniProtKB:P13482}. FT CARBOHYD 288 288 N-linked (GlcNAc...) asparagine. FT {ECO:0000255|PROSITE-ProRule:PRU00498}. FT CARBOHYD 293 293 N-linked (GlcNAc...) asparagine. FT {ECO:0000255|PROSITE-ProRule:PRU00498}. FT CARBOHYD 359 359 N-linked (GlcNAc...) asparagine. FT {ECO:0000255|PROSITE-ProRule:PRU00498}. FT CARBOHYD 451 451 N-linked (GlcNAc...) asparagine. FT {ECO:0000269|PubMed:17893096}. FT CARBOHYD 516 516 N-linked (GlcNAc...) asparagine. FT {ECO:0000255|PROSITE-ProRule:PRU00498}. FT VAR_SEQ 1 81 Missing (in isoform B). {ECO:0000305}. FT /FTId=VSP_021831. FT VAR_SEQ 1 53 MFKLPTISLLLVSWSCLVALSQAKTYSLPDLTTDYNNAIPV FT DEEEAQDPFASC -> MASPANPSSNHKMNGNG (in FT isoform A). FT {ECO:0000303|PubMed:12537569}. FT /FTId=VSP_007735. SQ SEQUENCE 596 AA; 67689 MW; 600D03B2087929EC CRC64; MFKLPTISLL LVSWSCLVAL SQAKTYSLPD LTTDYNNAIP VDEEEAQDPF ASCKIYCEGN LLHTIQTAVP KLFADSKTFV DMKLNNSPDK TLEDFNAMME AKNQTPSSED LKQFVDKYFS APGTELEKWT PTDWKENPSF LDLISDPDLK QWGVELNSIW KDLGRKMKDE VSKNPEYYSI IPVPNPVIVP GGRFIEFYYW DSYWIIRGLL YSQMFDTARG MIENFFSIVN RFGFIPNGGR VYYHGRSQPP LLTGMVKSYV DFTNDDKFAI DALDTLEHEF EFFVNNHNVT VKNHSLCVYR DSSSGPRPES YREDVETGEE FPTDEAKELH YSELKAGAES GMDFSSRWFI SPTGTNDGNR SALSTTSIVP VDLNAYLYWN AKLIAEFHSK AGNTKKVTEY ETKAEKLLLG IQEVLWNEEA GVWLDYDMIN QKPRDYYTPT NLSPLWVKAF NISESEKISA SVMAYIERNK LDSFPGGVPN TLSYTGEQWD APNVWAPMQY ILVEGLNNLN TPEAKNMSLK WATRWVKTNF AAFSKDRHMY EKYNADEFGV GGGGGEYEVQ TGFGWSNGVI IEWLSKHGRD ISIGSGCGCL AGEKRQ //