ID U520_DROME STANDARD; PRT; 2142 AA. AC Q9VUV9; Q8T9I9; DT 19-JUL-2003, integrated into UniProtKB/Swiss-Prot. DT 01-MAR-2003, sequence version 4. DT 07-FEB-2006, entry version 31. DE Putative U5 small nuclear ribonucleoprotein 200 kDa helicase DE (EC 3.6.1.-). GN ORFNames=CG5931; OS Drosophila melanogaster (Fruit fly). OC Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; OC Ephydroidea; Drosophilidae; Drosophila. OX NCBI_TaxID=7227; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley; RX MEDLINE=20196006; PubMed=10731132; DOI=10.1126/science.287.5461.2185; RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., RA Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., RA Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., RA Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., RA Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., RA Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., RA Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., RA Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., RA Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., RA de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., RA Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., RA Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., RA Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., RA Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., RA Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., RA Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., RA Kimmel B.E., Kodira C.D., Kraft C., Kravitz S., Kulp D., Lai Z., RA Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., RA Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., RA Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., RA Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., RA Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., RA Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., RA Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., RA Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T., RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., RA Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., RA Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., RA Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., RA Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., RA Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S., Zhu X., Smith H.O., RA Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.; RT "The genome sequence of Drosophila melanogaster."; RL Science 287:2185-2195(2000). RN [2] RP GENOME REANNOTATION. RX MEDLINE=22426069; PubMed=12537572; RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A., RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., RA Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., RA Lewis S.E.; RT "Annotation of the Drosophila melanogaster euchromatic genome: a RT systematic review."; RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1212-2130. RC STRAIN=Berkeley; TISSUE=Ovary; RX MEDLINE=22426066; PubMed=12537569; RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., RA George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., RA Rubin G.M., Celniker S.E.; RT "A Drosophila full-length cDNA resource."; RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002). CC -!- FUNCTION: Putative RNA helicase involved in the second step of RNA CC splicing (By similarity). CC -!- SUBCELLULAR LOCATION: Nucleus (Potential). CC -!- DOMAIN: Composed of two similar domains. CC -!- SIMILARITY: Belongs to the helicase family. SKI2 subfamily. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AE003529; AAF49564.4; -; Genomic_DNA. DR EMBL; AY069270; AAL39415.1; ALT_INIT; mRNA. DR Ensembl; CG5931; Drosophila melanogaster. DR FlyBase; FBgn0036548; CG5931. DR BioCyc; DMEL-XXX-02:DMEL-XXX-02-016835-MONOMER; -. DR GO; GO:0005681; C:spliceosome complex; ISS. DR GO; GO:0004004; F:ATP-dependent RNA helicase activity; ISS. DR GO; GO:0008380; P:RNA splicing; ISS. DR InterPro; IPR001410; DEAD. DR InterPro; IPR011545; DEAD/DEAH_N. DR InterPro; IPR001650; Helicase_C. DR InterPro; IPR004179; Sec63. DR Pfam; PF00270; DEAD; 2. DR Pfam; PF00271; Helicase_C; 2. DR Pfam; PF02889; Sec63; 2. DR SMART; SM00487; DEXDc; 2. DR SMART; SM00490; HELICc; 2. DR SMART; SM00611; SEC63; 2. KW ATP-binding; Complete proteome; Helicase; Hydrolase; KW Hypothetical protein; mRNA processing; mRNA splicing; Nuclear protein; KW Nucleotide-binding; Repeat; Spliceosome. FT CHAIN 1 2142 Putative U5 small nuclear FT ribonucleoprotein 200 kDa helicase. FT /FTId=PRO_0000102089. FT NP_BIND 503 510 ATP (Potential). FT NP_BIND 1350 1357 ATP (Potential). FT REGION 503 904 I. FT REGION 1350 1738 II. FT MOTIF 615 618 DEIH box. FT MOTIF 1453 1456 DELQ box. FT COMPBIAS 396 399 Poly-Ala. SQ SEQUENCE 2142 AA; 244510 MW; 10CFE9E84438F2BE CRC64; MADAAARQLQ YEYKANSNLV LQADVRLIER PRRDEATGEV CSLVGKLDGT RMGDRYQRTK PEKTEERKVK RQKRDEAQYD FERMKGATLL SEGIDEMVGI VYRPKTQETR QTYEVLLSFI QEALGDQPRD ILCGAADEIL AVLKNDRLKD RERKKDIDSL LGAVTDERFA LLVNLGKKIT DFGSDAVNAL TAAPNNEEQI DETYGINVQF EESEEESDND MYGEIRDDDA QDEGEEARID HTLHAENLAN EEAANNVKKE RSLHPLDIDA YWLQRCLSKF YKDAMVSQSK AADVLKILKD AADDRDCENQ LVLLLGYDCF DFIKQLKLNR QMVLYCTMLA SAQTDSERQR IREKMRGNSA LAKILRQLDT GKSEDQEEGE ARGSKRGKGD AEDGGAAAAG QVAGVRQLLE LEEMAFTQGS HFMANKRCQL PDGSYRKQRK GYEEVHVPAL KPVPFDANEE LQPVDKLPKY VQPVFEGFKT LNRIQSRLYK AALDSDENML LCAPTGAGKT NVALLTMMRE IGKHINEDGT INAQDFKIIY VAPMKSLVQE MVGNFGRRLA CYNLTVSELT GDHQLTREQI AATQVIVCTP EKWDIITRKG GERTFVSLVR LVIIDEIHLL HDERGPVLEA LVARTIRNIE TTQEEVRLVG LSATLPNYQD VATFLRVKPD KGLFYFDNSY RPVSLEQQYI GVTEKKALKR FQVMNEIVYE KTMEHAGRNQ VLVFVHSRKE TGKTARAVRD MCLEQDTLGS FLKEGSASME VLRTEAEQVK NTELKELLPY GFAIHHAGMT RVDRTLVEDL FADRHIQVLV STATLAWGVN LPAHTVIIKG TQVYNPEKGR WVELSALDVL QMLGRAGRPQ YDTKGEGILI TNHSELQFYL SLLNQQLPIE SQFISKLPDM LNAEIVLGTV QHLQDAVNWL GYTYLYIRML RNPTLYGVSH DAIKADPLLE QHRADLLHTA ACCLERSGLI KYDRKTGHFQ VTDLGRIASH YYLTHETMLT YNQLLKQTLS EIELFRVFSL SSEFRHISVR EEEKLELQKL MERVPIPIKE SIEEHSAKVN VLLQAYISQL KLEGFALMSD MVFITQSAAR LMRAIFEIVL TRGWAQLADK TLTLCKMIDR RMWQSMTPLR QFKKMPDEIA KKLEKKHFPW GRLYDLEPHE LGELIRVPKL GKTIHKFVHQ FPKLELSTHI QPITRGTLRV ELTITPDFQW DEKVHGQSEG FWVLIEDVDS ELILHHEFFL LKQKYSQDEH QLKFFVPVFE PLPPQYFLRI VSDRWIGAET QLPVSFRHLI LPEKNMPPTE LLDLQPLPIS ALRQPKFESF YSQRFPQFNP IQTQVFNAVY NSDENVFVGA PTGSGKMTIA EFAIMRLFTT QSDARCVYLV SEEALADLVF ADWHSKFGSL DIKVVKLTGE TGTDLKLIAK GQLVITTADK WDVLSRRWKQ RKNVQLVNLF IVDELQLVGG EEGPVLEIVC SRMRYISSQI EKQIRIVALS ASLTDARDVA QWLGCNPNAT FNFHPSVRPI PLELHIQGYN VTHNATRIAT MSKPVYNAIL KYSAHKPVIV FVSSRKQARL TAIDVLTYAA SDLQPNRFFH AEEEDIKPFL ERMTDKTLKE TLAQGVAYLH EGLSASDHRL VEQLFDSGAV QVAVISRDLC WGMSISAHLV IIMDTQFYNG KNHSYEDYPI TDVLQMIGRA NRPNEDADAK CVLMCQSSKK DFFKKFINEP LPIESHLDHR MHDHFNAEVV TKTIENKQDA VDYLTWTFLY RRLTQNPNYY NLQGVTHRHL SDHLSELVEN TLSDLEQSKC ISVEDDMDTL PLNLGMIAAY YYINYTTIEL FSLSLNSKTK VRGLLEIISS AAEYEDVVVR HHEEQVLRTL SQRLPNKLTG PNETAPKFND PHIKTNLLLQ AHLSRLQLGP ELQGDTEQIL SKAIRLIQAC VDVLSSNGWL SPAVAAMELA QMVTQAMWSK DSYLKQLPHF SPEIVKRCTE KKIETVFDIM ELEDEDRTRL LQLSDLQMAD VARFCNRYPN IELNYEVVDK DRINSGSTVN VVVQLEREDE VTGPVIAPFF PQKREEGWWV VIGDPKTNSL LSIKRLTLQQ KAKVKLDFVA PSPGKHDYTL YYMSDSYLGC DQEYKFSIEV GDFQSESESE SD //