ID U520_DROME Reviewed; 2142 AA. AC Q9VUV9; Q5U0X1; Q8T9I9; DT 19-JUL-2003, integrated into UniProtKB/Swiss-Prot. DT 01-MAR-2003, sequence version 4. DT 23-MAY-2018, entry version 138. DE RecName: Full=Putative U5 small nuclear ribonucleoprotein 200 kDa helicase; DE EC=3.6.4.13; DE AltName: Full=Protein lethal (3) 72Ab; GN Name=l(3)72Ab; ORFNames=CG5931; OS Drosophila melanogaster (Fruit fly). OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; OC Pterygota; Neoptera; Holometabola; Diptera; Brachycera; Muscomorpha; OC Ephydroidea; Drosophilidae; Drosophila; Sophophora. OX NCBI_TaxID=7227; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley; RX PubMed=10731132; DOI=10.1126/science.287.5461.2185; RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., RA Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., RA Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., RA Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., RA Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., RA Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., RA Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., RA Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., RA Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., RA de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., RA Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., RA Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., RA Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., RA Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., RA Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., RA Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., RA Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., RA Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., RA Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., RA Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., RA Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., RA Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., RA Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., RA Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., RA Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., RA Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., RA Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., RA Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., RA Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., RA Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., RA Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.; RT "The genome sequence of Drosophila melanogaster."; RL Science 287:2185-2195(2000). RN [2] RP GENOME REANNOTATION. RC STRAIN=Berkeley; RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083; RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A., RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., RA Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., RA Lewis S.E.; RT "Annotation of the Drosophila melanogaster euchromatic genome: a RT systematic review."; RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=Berkeley; TISSUE=Embryo; RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., RA Pacleb J.M., Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.; RL Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases. RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1212-2130. RC STRAIN=Berkeley; TISSUE=Ovary; RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080; RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., RA George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., RA Rubin G.M., Celniker S.E.; RT "A Drosophila full-length cDNA resource."; RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002). CC -!- FUNCTION: Putative RNA helicase involved in the second step of RNA CC splicing. {ECO:0000250}. CC -!- CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate. CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}. CC -!- DOMAIN: Composed of two similar domains. CC -!- SIMILARITY: Belongs to the helicase family. SKI2 subfamily. CC {ECO:0000305}. CC -!- SEQUENCE CAUTION: CC Sequence=AAL39415.1; Type=Erroneous initiation; Evidence={ECO:0000305}; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AE014296; AAF49564.4; -; Genomic_DNA. DR EMBL; BT016121; AAV37006.1; -; mRNA. DR EMBL; AY069270; AAL39415.1; ALT_INIT; mRNA. DR RefSeq; NP_648818.3; NM_140561.4. DR UniGene; Dm.973; -. DR ProteinModelPortal; Q9VUV9; -. DR SMR; Q9VUV9; -. DR BioGrid; 65049; 18. DR IntAct; Q9VUV9; 7. DR MINT; Q9VUV9; -. DR STRING; 7227.FBpp0075282; -. DR PaxDb; Q9VUV9; -. DR PRIDE; Q9VUV9; -. DR EnsemblMetazoa; FBtr0075527; FBpp0075282; FBgn0263599. DR GeneID; 39737; -. DR KEGG; dme:Dmel_CG5931; -. DR FlyBase; FBgn0263599; l(3)72Ab. DR eggNOG; KOG0952; Eukaryota. DR eggNOG; COG1204; LUCA. DR GeneTree; ENSGT00640000091272; -. DR InParanoid; Q9VUV9; -. DR KO; K12854; -. DR OMA; TLCKMID; -. DR OrthoDB; EOG091G0HE5; -. DR PhylomeDB; Q9VUV9; -. DR Reactome; R-DME-72163; mRNA Splicing - Major Pathway. DR Reactome; R-DME-72165; mRNA Splicing - Minor Pathway. DR ChiTaRS; l(3)72Ab; fly. DR GenomeRNAi; 39737; -. DR PRO; PR:Q9VUV9; -. DR Proteomes; UP000000803; Chromosome 3L. DR Bgee; FBgn0263599; -. DR Genevisible; Q9VUV9; DM. DR GO; GO:0071013; C:catalytic step 2 spliceosome; HDA:FlyBase. DR GO; GO:0005634; C:nucleus; IDA:FlyBase. DR GO; GO:0071011; C:precatalytic spliceosome; HDA:FlyBase. DR GO; GO:0030532; C:small nuclear ribonucleoprotein complex; IPI:FlyBase. DR GO; GO:0005681; C:spliceosomal complex; ISS:UniProtKB. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0004004; F:ATP-dependent RNA helicase activity; ISS:FlyBase. DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro. DR GO; GO:0003724; F:RNA helicase activity; ISS:FlyBase. DR GO; GO:0000278; P:mitotic cell cycle; HMP:FlyBase. DR GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:UniProtKB. DR GO; GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; IMP:FlyBase. DR GO; GO:0008380; P:RNA splicing; ISS:FlyBase. DR GO; GO:0007283; P:spermatogenesis; IMP:FlyBase. DR GO; GO:0007419; P:ventral cord development; HMP:FlyBase. DR CDD; cd00079; HELICc; 1. DR Gene3D; 2.60.40.150; -; 2. DR InterPro; IPR035892; C2_domain_sf. DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom. DR InterPro; IPR014001; Helicase_ATP-bd. DR InterPro; IPR001650; Helicase_C. DR InterPro; IPR014756; Ig_E-set. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR004179; Sec63-dom. DR InterPro; IPR036390; WH_DNA-bd_sf. DR Pfam; PF00270; DEAD; 2. DR Pfam; PF00271; Helicase_C; 1. DR Pfam; PF02889; Sec63; 2. DR SMART; SM00487; DEXDc; 2. DR SMART; SM00490; HELICc; 2. DR SMART; SM00973; Sec63; 2. DR SUPFAM; SSF46785; SSF46785; 2. DR SUPFAM; SSF52540; SSF52540; 4. DR SUPFAM; SSF81296; SSF81296; 1. DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 2. DR PROSITE; PS51194; HELICASE_CTER; 2. PE 2: Evidence at transcript level; KW ATP-binding; Complete proteome; Helicase; Hydrolase; mRNA processing; KW mRNA splicing; Nucleotide-binding; Nucleus; Reference proteome; KW Repeat; Spliceosome. FT CHAIN 1 2142 Putative U5 small nuclear FT ribonucleoprotein 200 kDa helicase. FT /FTId=PRO_0000102089. FT DOMAIN 490 673 Helicase ATP-binding 1. FT {ECO:0000255|PROSITE-ProRule:PRU00541}. FT DOMAIN 684 917 Helicase C-terminal 1. FT {ECO:0000255|PROSITE-ProRule:PRU00542}. FT DOMAIN 981 1286 SEC63 1. FT DOMAIN 1337 1511 Helicase ATP-binding 2. FT {ECO:0000255|PROSITE-ProRule:PRU00541}. FT DOMAIN 1544 1752 Helicase C-terminal 2. FT {ECO:0000255|PROSITE-ProRule:PRU00542}. FT DOMAIN 1811 2128 SEC63 2. FT NP_BIND 503 510 ATP. {ECO:0000255|PROSITE- FT ProRule:PRU00541}. FT NP_BIND 1350 1357 ATP. {ECO:0000255|PROSITE- FT ProRule:PRU00541}. FT MOTIF 615 618 DEIH box. FT MOTIF 1453 1456 DELQ box. FT COMPBIAS 396 399 Poly-Ala. SQ SEQUENCE 2142 AA; 244510 MW; 10CFE9E84438F2BE CRC64; MADAAARQLQ YEYKANSNLV LQADVRLIER PRRDEATGEV CSLVGKLDGT RMGDRYQRTK PEKTEERKVK RQKRDEAQYD FERMKGATLL SEGIDEMVGI VYRPKTQETR QTYEVLLSFI QEALGDQPRD ILCGAADEIL AVLKNDRLKD RERKKDIDSL LGAVTDERFA LLVNLGKKIT DFGSDAVNAL TAAPNNEEQI DETYGINVQF EESEEESDND MYGEIRDDDA QDEGEEARID HTLHAENLAN EEAANNVKKE RSLHPLDIDA YWLQRCLSKF YKDAMVSQSK AADVLKILKD AADDRDCENQ LVLLLGYDCF DFIKQLKLNR QMVLYCTMLA SAQTDSERQR IREKMRGNSA LAKILRQLDT GKSEDQEEGE ARGSKRGKGD AEDGGAAAAG QVAGVRQLLE LEEMAFTQGS HFMANKRCQL PDGSYRKQRK GYEEVHVPAL KPVPFDANEE LQPVDKLPKY VQPVFEGFKT LNRIQSRLYK AALDSDENML LCAPTGAGKT NVALLTMMRE IGKHINEDGT INAQDFKIIY VAPMKSLVQE MVGNFGRRLA CYNLTVSELT GDHQLTREQI AATQVIVCTP EKWDIITRKG GERTFVSLVR LVIIDEIHLL HDERGPVLEA LVARTIRNIE TTQEEVRLVG LSATLPNYQD VATFLRVKPD KGLFYFDNSY RPVSLEQQYI GVTEKKALKR FQVMNEIVYE KTMEHAGRNQ VLVFVHSRKE TGKTARAVRD MCLEQDTLGS FLKEGSASME VLRTEAEQVK NTELKELLPY GFAIHHAGMT RVDRTLVEDL FADRHIQVLV STATLAWGVN LPAHTVIIKG TQVYNPEKGR WVELSALDVL QMLGRAGRPQ YDTKGEGILI TNHSELQFYL SLLNQQLPIE SQFISKLPDM LNAEIVLGTV QHLQDAVNWL GYTYLYIRML RNPTLYGVSH DAIKADPLLE QHRADLLHTA ACCLERSGLI KYDRKTGHFQ VTDLGRIASH YYLTHETMLT YNQLLKQTLS EIELFRVFSL SSEFRHISVR EEEKLELQKL MERVPIPIKE SIEEHSAKVN VLLQAYISQL KLEGFALMSD MVFITQSAAR LMRAIFEIVL TRGWAQLADK TLTLCKMIDR RMWQSMTPLR QFKKMPDEIA KKLEKKHFPW GRLYDLEPHE LGELIRVPKL GKTIHKFVHQ FPKLELSTHI QPITRGTLRV ELTITPDFQW DEKVHGQSEG FWVLIEDVDS ELILHHEFFL LKQKYSQDEH QLKFFVPVFE PLPPQYFLRI VSDRWIGAET QLPVSFRHLI LPEKNMPPTE LLDLQPLPIS ALRQPKFESF YSQRFPQFNP IQTQVFNAVY NSDENVFVGA PTGSGKMTIA EFAIMRLFTT QSDARCVYLV SEEALADLVF ADWHSKFGSL DIKVVKLTGE TGTDLKLIAK GQLVITTADK WDVLSRRWKQ RKNVQLVNLF IVDELQLVGG EEGPVLEIVC SRMRYISSQI EKQIRIVALS ASLTDARDVA QWLGCNPNAT FNFHPSVRPI PLELHIQGYN VTHNATRIAT MSKPVYNAIL KYSAHKPVIV FVSSRKQARL TAIDVLTYAA SDLQPNRFFH AEEEDIKPFL ERMTDKTLKE TLAQGVAYLH EGLSASDHRL VEQLFDSGAV QVAVISRDLC WGMSISAHLV IIMDTQFYNG KNHSYEDYPI TDVLQMIGRA NRPNEDADAK CVLMCQSSKK DFFKKFINEP LPIESHLDHR MHDHFNAEVV TKTIENKQDA VDYLTWTFLY RRLTQNPNYY NLQGVTHRHL SDHLSELVEN TLSDLEQSKC ISVEDDMDTL PLNLGMIAAY YYINYTTIEL FSLSLNSKTK VRGLLEIISS AAEYEDVVVR HHEEQVLRTL SQRLPNKLTG PNETAPKFND PHIKTNLLLQ AHLSRLQLGP ELQGDTEQIL SKAIRLIQAC VDVLSSNGWL SPAVAAMELA QMVTQAMWSK DSYLKQLPHF SPEIVKRCTE KKIETVFDIM ELEDEDRTRL LQLSDLQMAD VARFCNRYPN IELNYEVVDK DRINSGSTVN VVVQLEREDE VTGPVIAPFF PQKREEGWWV VIGDPKTNSL LSIKRLTLQQ KAKVKLDFVA PSPGKHDYTL YYMSDSYLGC DQEYKFSIEV GDFQSESESE SD //