ID SCLLA_DROME Reviewed; 280 AA. AC Q9VTH4; B9EQR6; Q5U1B7; Q8SY44; Q9NHN4; DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot. DT 01-MAY-2000, sequence version 1. DT 20-APR-2010, entry version 51. DE RecName: Full=Protein scylla; DE AltName: Full=Protein regulated in development and DNA damage response 2; DE Short=REDD-2; GN Name=scyl; ORFNames=CG7590; OS Drosophila melanogaster (Fruit fly). OC Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; OC Ephydroidea; Drosophilidae; Drosophila; Sophophora. OX NCBI_TaxID=7227; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A). RA Chauvet S., Maurel-Zaffran C., Miassod R., Jullien N., Pradel J., RA Aragnol D.; RT "Characterization of charybde and scylla, two paralogous target genes RT of Hox and cofactors proteins in Drosophila."; RL Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases. RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley; RX MEDLINE=20196006; PubMed=10731132; DOI=10.1126/science.287.5461.2185; RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., RA Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., RA Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., RA Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., RA Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., RA Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., RA Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., RA Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., RA Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., RA de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., RA Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., RA Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., RA Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., RA Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., RA Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., RA Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., RA Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., RA Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., RA Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., RA Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., RA Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., RA Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., RA Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., RA Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., RA Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., RA Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., RA Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., RA Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., RA Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., RA Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., RA Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.; RT "The genome sequence of Drosophila melanogaster."; RL Science 287:2185-2195(2000). RN [3] RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING (ISOFORMS A AND B). RX MEDLINE=22426069; PubMed=12537572; RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A., RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., RA Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., RA Lewis S.E.; RT "Annotation of the Drosophila melanogaster euchromatic genome: a RT systematic review."; RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS A AND B). RC STRAIN=Berkeley; TISSUE=Embryo; RX MEDLINE=22426066; PubMed=12537569; RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., RA George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., RA Rubin G.M., Celniker S.E.; RT "A Drosophila full-length cDNA resource."; RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002). RN [5] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). RC STRAIN=Berkeley; RA Carlson J.W., Booth B., Frise E., Park S., Wan K.H., Yu C., RA Celniker S.E.; RL Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases. RN [6] RP IDENTIFICATION. RX PubMed=12453409; DOI=10.1016/S1097-2765(02)00706-2; RA Ellisen L.W., Ramsayer K.D., Johannessen C.M., Yang A., Beppu H., RA Minda K., Oliner J.D., McKeon F., Haber D.A.; RT "REDD1, a developmentally regulated transcriptional target of p63 and RT p53, links p63 to regulation of reactive oxygen species."; RL Mol. Cell 10:995-1005(2002). RN [7] RP FUNCTION, AND INDUCTION. RX PubMed=15545626; DOI=10.1101/gad.322704; RA Reiling J.H., Hafen E.; RT "The hypoxia-induced paralogs Scylla and Charybdis inhibit growth by RT down-regulating S6K activity upstream of TSC in Drosophila."; RL Genes Dev. 18:2879-2892(2004). RN [8] RP FUNCTION. RX PubMed=15632201; DOI=10.1074/jbc.C400557200; RA Corradetti M.N., Inoki K., Guan K.-L.; RT "The stress-inducted proteins RTP801 and RTP801L are negative RT regulators of the mammalian target of rapamycin pathway."; RL J. Biol. Chem. 280:9769-9772(2005). RN [9] RP FUNCTION, INDUCTION, AND DEVELOPMENTAL STAGE. RX PubMed=16423342; DOI=10.1016/j.ydbio.2005.12.014; RA Scuderi A., Simin K., Kazuko S.G., Metherall J.E., Letsou A.; RT "scylla and charybde, homologues of the human apoptotic gene RTP801, RT are required for head involution in Drosophila."; RL Dev. Biol. 291:110-122(2006). CC -!- FUNCTION: Inhibits cell growth by regulating the Tor pathway CC upstream of the Tsc1-Tsc2 complex and downstream of Akt1. Acts as CC cell death activator during head development. CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity). CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=2; CC Name=A; CC IsoId=Q9VTH4-1; Sequence=Displayed; CC Name=B; CC IsoId=Q9VTH4-2; Sequence=VSP_028636; CC -!- DEVELOPMENTAL STAGE: Expression peaks 2-8 hours after egg laying. CC Expressed in the dorsal domains of gastrulation stage embryos. CC During mid-embryonic developmental stages, expressed in the CC central nervous system, the three thoracic segments and the CC cardiac precursor cells. CC -!- INDUCTION: Expression is controlled by homeobox transcription CC factors. At larval stages, induced by starvation, and by hypoxia CC in midgut and fat body. CC -!- SIMILARITY: Belongs to the DDIT4 family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AF221110; AAF59841.1; -; mRNA. DR EMBL; AE014296; AAF50074.1; -; Genomic_DNA. DR EMBL; AE014296; AAS65038.1; -; Genomic_DNA. DR EMBL; AY075382; AAL68220.1; -; mRNA. DR EMBL; BT015975; AAV36860.1; -; mRNA. DR EMBL; BT057991; ACM16701.1; -; mRNA. DR RefSeq; NP_648456.2; -. DR RefSeq; NP_996043.1; -. DR UniGene; Dm.6574; -. DR IntAct; Q9VTH4; 6. DR STRING; Q9VTH4; -. DR PRIDE; Q9VTH4; -. DR Ensembl; FBtr0076168; FBpp0075899; FBgn0041094; Drosophila melanogaster. DR Ensembl; FBtr0076169; FBpp0089347; FBgn0041094; Drosophila melanogaster. DR GeneID; 39270; -. DR KEGG; dme:Dmel_CG7590; -. DR UCSC; CG7590-RA; d. melanogaster. DR CTD; 39270; -. DR FlyBase; FBgn0041094; scyl. DR eggNOG; inNOG10804; -. DR InParanoid; Q9VTH4; -. DR OMA; LARTITI; -. DR OrthoDB; EOG9M91Z2; -. DR PhylomeDB; Q9VTH4; -. DR NextBio; 812820; -. DR Bgee; Q9VTH4; -. DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB. DR GO; GO:0006915; P:apoptosis; IMP:UniProtKB. DR GO; GO:0008258; P:head involution; IMP:UniProtKB. DR GO; GO:0045926; P:negative regulation of growth; IGI:FlyBase. DR GO; GO:0009968; P:negative regulation of signal transduction; IEA:InterPro. DR GO; GO:0032006; P:regulation of TOR signaling pathway; IMP:UniProtKB. DR GO; GO:0006974; P:response to DNA damage stimulus; IMP:FlyBase. DR GO; GO:0006979; P:response to oxidative stress; IMP:UniProtKB. DR InterPro; IPR012918; RTP801_C. DR PANTHER; PTHR12478; RTP801_C; 1. DR Pfam; PF07809; RTP801_C; 1. PE 2: Evidence at transcript level; KW Alternative splicing; Apoptosis; Complete proteome; Cytoplasm; KW Developmental protein. FT CHAIN 1 280 Protein scylla. FT /FTId=PRO_0000307211. FT VAR_SEQ 1 39 Missing (in isoform B). FT /FTId=VSP_028636. FT CONFLICT 21 21 K -> E (in Ref. 4; AAV36860). FT CONFLICT 203 203 S -> P (in Ref. 1; AAF59841). SQ SEQUENCE 280 AA; 30762 MW; 894841884CD28258 CRC64; MKMDVIAREQ IIYGSLQGSN KNKDWTSRLP PPSAYTLDLM SKKAKTTTGG SSNGSNATAT STTTSTSSSI KHKQPAGSSN NNVGQSQSKK TKPSGSYNSN SNYYYYAADE EEGGSADYAL SNYDKKAVEE LSLRLLDELR AAKSRHLTCT EVSLPCDLTP SVAREIIRVS EKEPRGIRGC TIYIEFEDEP KNSRRIASIK VDSDTVSTFE VYLTLRQDHR GWTSLLPQFM KSLARTITIS PEYTITKNKL YSADGLGARR SYSFGSHAHR PSAAIATPTN //