ID LIN28_DROME Reviewed; 195 AA. AC Q9VRN5; DT 17-OCT-2006, integrated into UniProtKB/Swiss-Prot. DT 01-OCT-2002, sequence version 2. DT 13-SEP-2023, entry version 156. DE RecName: Full=Protein lin-28 homolog; GN Name=lin-28; ORFNames=CG17334; OS Drosophila melanogaster (Fruit fly). OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; OC Drosophilidae; Drosophila; Sophophora. OX NCBI_TaxID=7227; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE. RX PubMed=12798299; DOI=10.1016/s0012-1606(03)00126-x; RA Moss E.G., Tang L.; RT "Conservation of the heterochronic regulator Lin-28, its developmental RT expression and microRNA complementary sites."; RL Dev. Biol. 258:432-442(2003). RN [2] RP ERRATUM OF PUBMED:12798299. RA Moss E.G., Tang L.; RL Dev. Biol. 262:361-361(2003). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley; RX PubMed=10731132; DOI=10.1126/science.287.5461.2185; RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C., RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., RA Venter J.C.; RT "The genome sequence of Drosophila melanogaster."; RL Science 287:2185-2195(2000). RN [4] RP GENOME REANNOTATION. RC STRAIN=Berkeley; RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083; RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A., RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.; RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic RT review."; RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002). RN [5] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=Berkeley; RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., RA Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A., RA Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., RA Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.; RL Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases. CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. CC -!- DEVELOPMENTAL STAGE: Expressed from embryogenesis to the first instar CC larval stage, then at the pupal stage. Not expressed at significant CC levels in the adult. {ECO:0000269|PubMed:12798299}. CC -!- SIMILARITY: Belongs to the lin-28 family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AF521096; AAM77748.1; -; mRNA. DR EMBL; AE014296; AAF50758.2; -; Genomic_DNA. DR EMBL; AY118360; AAM48389.1; -; mRNA. DR RefSeq; NP_647983.1; NM_139726.2. DR AlphaFoldDB; Q9VRN5; -. DR SMR; Q9VRN5; -. DR BioGRID; 64102; 5. DR IntAct; Q9VRN5; 5. DR STRING; 7227.FBpp0076798; -. DR PaxDb; Q9VRN5; -. DR DNASU; 38639; -. DR EnsemblMetazoa; FBtr0077090; FBpp0076798; FBgn0035626. DR GeneID; 38639; -. DR KEGG; dme:Dmel_CG17334; -. DR UCSC; CG17334-RA; d. melanogaster. DR AGR; FB:FBgn0035626; -. DR CTD; 38639; -. DR FlyBase; FBgn0035626; lin-28. DR VEuPathDB; VectorBase:FBgn0035626; -. DR eggNOG; KOG3070; Eukaryota. DR GeneTree; ENSGT00940000171032; -. DR HOGENOM; CLU_089169_4_0_1; -. DR InParanoid; Q9VRN5; -. DR OMA; HTVASCP; -. DR OrthoDB; 5487692at2759; -. DR PhylomeDB; Q9VRN5; -. DR SignaLink; Q9VRN5; -. DR BioGRID-ORCS; 38639; 0 hits in 3 CRISPR screens. DR GenomeRNAi; 38639; -. DR PRO; PR:Q9VRN5; -. DR Proteomes; UP000000803; Chromosome 3L. DR Bgee; FBgn0035626; Expressed in embryonic/larval lymph gland and 35 other tissues. DR Genevisible; Q9VRN5; DM. DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central. DR GO; GO:0010494; C:cytoplasmic stress granule; IDA:FlyBase. DR GO; GO:0005829; C:cytosol; IDA:FlyBase. DR GO; GO:0005634; C:nucleus; IBA:GO_Central. DR GO; GO:0003730; F:mRNA 3'-UTR binding; IDA:FlyBase. DR GO; GO:0003729; F:mRNA binding; IDA:FlyBase. DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro. DR GO; GO:0070935; P:3'-UTR-mediated mRNA stabilization; IDA:FlyBase. DR GO; GO:0060250; P:germ-line stem-cell niche homeostasis; IMP:FlyBase. DR GO; GO:2000632; P:negative regulation of pre-miRNA processing; IMP:FlyBase. DR GO; GO:0048477; P:oogenesis; IMP:FlyBase. DR GO; GO:0046628; P:positive regulation of insulin receptor signaling pathway; IMP:FlyBase. DR GO; GO:0046427; P:positive regulation of receptor signaling pathway via JAK-STAT; IMP:FlyBase. DR GO; GO:2000648; P:positive regulation of stem cell proliferation; IMP:FlyBase. DR GO; GO:0048621; P:post-embryonic digestive tract morphogenesis; IMP:FlyBase. DR GO; GO:0010608; P:post-transcriptional regulation of gene expression; IMP:FlyBase. DR GO; GO:0031054; P:pre-miRNA processing; IBA:GO_Central. DR GO; GO:0098724; P:symmetric stem cell division; IMP:FlyBase. DR CDD; cd04458; CSP_CDS; 1. DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1. DR Gene3D; 4.10.60.10; Zinc finger, CCHC-type; 1. DR InterPro; IPR011129; CSD. DR InterPro; IPR002059; CSP_DNA-bd. DR InterPro; IPR012340; NA-bd_OB-fold. DR InterPro; IPR001878; Znf_CCHC. DR InterPro; IPR036875; Znf_CCHC_sf. DR PANTHER; PTHR46109; PROTEIN LIN-28; 1. DR PANTHER; PTHR46109:SF1; PROTEIN LIN-28 HOMOLOG; 1. DR Pfam; PF00313; CSD; 1. DR PRINTS; PR00050; COLDSHOCK. DR SMART; SM00357; CSP; 1. DR SMART; SM00343; ZnF_C2HC; 2. DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1. DR SUPFAM; SSF57756; Retrovirus zinc finger-like domains; 1. DR PROSITE; PS51857; CSD_2; 1. PE 2: Evidence at transcript level; KW Cytoplasm; Metal-binding; Reference proteome; Repeat; Zinc; Zinc-finger. FT CHAIN 1..195 FT /note="Protein lin-28 homolog" FT /id="PRO_0000253797" FT DOMAIN 38..104 FT /note="CSD" FT ZN_FING 126..143 FT /note="CCHC-type 1" FT ZN_FING 148..165 FT /note="CCHC-type 2" FT REGION 1..31 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 164..195 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 1..29 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 166..195 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT BINDING 127 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /ligand_label="1" FT /evidence="ECO:0000250" FT BINDING 130 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /ligand_label="1" FT /evidence="ECO:0000250" FT BINDING 136 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /ligand_label="1" FT /evidence="ECO:0000250" FT BINDING 141 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /ligand_label="1" FT /evidence="ECO:0000250" FT BINDING 150 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /ligand_label="2" FT /evidence="ECO:0000250" FT BINDING 153 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /ligand_label="2" FT /evidence="ECO:0000250" FT BINDING 158 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /ligand_label="2" FT /evidence="ECO:0000250" FT BINDING 163 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /ligand_label="2" FT /evidence="ECO:0000250" SQ SEQUENCE 195 AA; 21594 MW; 4F2E080F20137EA5 CRC64; MENVQLENGL ERRTTSQSST SSANPANLAS PTEECGCVRL GKCKWFNVAK GWGFLTPNDG GQEVFVHQSV IQMSGFRSLG EQEEVEFECQ RTSRGLEATR VSSRHGGSCQ GSTYRPRINR RTRRMRCYNC GEFANHIASE CALGPQPKRC HRCRGEDHLH ADCPHKNVTQ SHSNSKSISN NSSSSAAQEK SEEAT //