ID Q9VMR2_DROME Unreviewed; 580 AA. AC Q9VMR2; DT 01-MAY-2000, integrated into UniProtKB/TrEMBL. DT 01-MAY-2000, sequence version 1. DT 08-NOV-2023, entry version 199. DE SubName: Full=LP04777p {ECO:0000313|EMBL:AAZ41777.1}; DE SubName: Full=Midline, isoform A {ECO:0000313|EMBL:AAF52250.1}; DE SubName: Full=Midline, isoform B {ECO:0000313|EMBL:AGB92632.1}; GN Name=mid {ECO:0000313|EMBL:AAF52250.1, GN ECO:0000313|FlyBase:FBgn0261963}; GN Synonyms=Dmel\CG6634 {ECO:0000313|EMBL:AAF52250.1}, extra GN {ECO:0000313|EMBL:AAF52250.1}, H15 {ECO:0000313|EMBL:AAF52250.1}, H15r GN {ECO:0000313|EMBL:AAF52250.1}, H15r/nmr2 GN {ECO:0000313|EMBL:AAF52250.1}, los {ECO:0000313|EMBL:AAF52250.1}, MID GN {ECO:0000313|EMBL:AAF52250.1}, Mid {ECO:0000313|EMBL:AAF52250.1}, GN Mid(H15) {ECO:0000313|EMBL:AAF52250.1}, nmr2 GN {ECO:0000313|EMBL:AAF52250.1}, TBX20 {ECO:0000313|EMBL:AAF52250.1}; GN ORFNames=CG6634 {ECO:0000313|EMBL:AAF52250.1, GN ECO:0000313|FlyBase:FBgn0261963}, Dmel_CG6634 GN {ECO:0000313|EMBL:AAF52250.1}; OS Drosophila melanogaster (Fruit fly). OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; OC Drosophilidae; Drosophila; Sophophora. OX NCBI_TaxID=7227 {ECO:0000313|EMBL:AAF52250.1, ECO:0000313|Proteomes:UP000000803}; RN [1] {ECO:0000313|EMBL:AAF52250.1, ECO:0000313|Proteomes:UP000000803} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803}; RX PubMed=10731132; DOI=10.1126/science.287.5461.2185; RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C., RA Rogers Y.H., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., RA Baxter E.G., Helt G., Nelson C.R., Gabor G.L., Abril J.F., Agbayani A., RA An H.J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., RA Harris N.L., Harvey D., Heiman T.J., Hernandez J.R., Houck J., Hostin D., RA Houston K.A., Howland T.J., Wei M.H., Ibegwam C., Jalali M., Kalush F., RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., RA Kraft C., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., RA Li J., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., RA Reese M.G., Reinert K., Remington K., Saunders R.D., Scheeler F., Shen H., RA Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T., Spier E., RA Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., RA Turner R., Venter E., Wang A.H., Wang X., Wang Z.Y., Wassarman D.A., RA Weinstock G.M., Weissenbach J., Williams S.M., WoodageT, Worley K.C., RA Wu D., Yang S., Yao Q.A., Ye J., Yeh R.F., Zaveri J.S., Zhan M., Zhang G., RA Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S., RA Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.; RT "The genome sequence of Drosophila melanogaster."; RL Science 287:2185-2195(2000). RN [2] {ECO:0000313|Proteomes:UP000000803} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803}; RX PubMed=12537568; RA Celniker S.E., Wheeler D.A., Kronmiller B., Carlson J.W., Halpern A., RA Patel S., Adams M., Champe M., Dugan S.P., Frise E., Hodgson A., RA George R.A., Hoskins R.A., Laverty T., Muzny D.M., Nelson C.R., RA Pacleb J.M., Park S., Pfeiffer B.D., Richards S., Sodergren E.J., RA Svirskas R., Tabor P.E., Wan K., Stapleton M., Sutton G.G., Venter C., RA Weinstock G., Scherer S.E., Myers E.W., Gibbs R.A., Rubin G.M.; RT "Finishing a whole-genome shotgun: release 3 of the Drosophila melanogaster RT euchromatic genome sequence."; RL Genome Biol. 3:RESEARCH0079-RESEARCH0079(2002). RN [3] {ECO:0000313|Proteomes:UP000000803} RP GENOME REANNOTATION. RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803}; RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083; RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A., RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.; RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic RT review."; RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002). RN [4] {ECO:0000313|Proteomes:UP000000803} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803}; RX PubMed=12537573; RA Kaminker J.S., Bergman C.M., Kronmiller B., Carlson J., Svirskas R., RA Patel S., Frise E., Wheeler D.A., Lewis S.E., Rubin G.M., Ashburner M., RA Celniker S.E.; RT "The transposable elements of the Drosophila melanogaster euchromatin: a RT genomics perspective."; RL Genome Biol. 3:RESEARCH0084.1-RESEARCH0084.20(2002). RN [5] {ECO:0000313|EMBL:AAF52250.1, ECO:0000313|Proteomes:UP000000803} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803}; RX PubMed=12537574; RA Hoskins R.A., Smith C.D., Carlson J.W., Carvalho A.B., Halpern A., RA Kaminker J.S., Kennedy C., Mungall C.J., Sullivan B.A., Sutton G.G., RA Yasuhara J.C., Wakimoto B.T., Myers E.W., Celniker S.E., Rubin G.M., RA Karpen G.H.; RT "Heterochromatic sequences in a Drosophila whole-genome shotgun assembly."; RL Genome Biol. 3:RESEARCH0085-RESEARCH0085(2002). RN [6] {ECO:0000313|EMBL:AAF52250.1, ECO:0000313|Proteomes:UP000000803} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803}; RX PubMed=16110336; DOI=10.1371/journal.pcbi.0010022; RA Quesneville H., Bergman C.M., Andrieu O., Autard D., Nouaud D., RA Ashburner M., Anxolabehere D.; RT "Combined evidence annotation of transposable elements in genome RT sequences."; RL PLoS Comput. Biol. 1:166-175(2005). RN [7] {ECO:0000313|EMBL:AAZ41777.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=Berkeley {ECO:0000313|EMBL:AAZ41777.1}; RA Stapleton M., Carlson J., Chavez C., Frise E., George R., Pacleb J., RA Park S., Wan K., Yu C., Celniker S.; RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases. RN [8] {ECO:0000313|EMBL:AAF52250.1} RP NUCLEOTIDE SEQUENCE. RA Celniker S., Carlson J., Wan K., Frise E., Hoskins R., Park S., RA Svirskas R., Rubin G.; RL Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases. RN [9] {ECO:0000313|EMBL:AAF52250.1, ECO:0000313|Proteomes:UP000000803} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803}; RX PubMed=17569856; DOI=10.1126/science.1139815; RA Smith C.D., Shu S., Mungall C.J., Karpen G.H.; RT "The Release 5.1 annotation of Drosophila melanogaster heterochromatin."; RL Science 316:1586-1591(2007). RN [10] {ECO:0000313|EMBL:AAF52250.1, ECO:0000313|Proteomes:UP000000803} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803}; RX PubMed=17569867; DOI=10.1126/science.1139816; RA Hoskins R.A., Carlson J.W., Kennedy C., Acevedo D., Evans-Holm M., RA Frise E., Wan K.H., Park S., Mendez-Lago M., Rossi F., Villasante A., RA Dimitri P., Karpen G.H., Celniker S.E.; RT "Sequence finishing and mapping of Drosophila melanogaster RT heterochromatin."; RL Science 316:1625-1628(2007). RN [11] {ECO:0000313|EMBL:AAF52250.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=26109357; DOI=.1534/g3.115.018929; RG FlyBase Consortium; RA Matthews B.B., Dos Santos G., Crosby M.A., Emmert D.B., St Pierre S.E., RA Gramates L.S., Zhou P., Schroeder A.J., Falls K., Strelets V., Russo S.M., RA Gelbart W.M., null; RT "Gene Model Annotations for Drosophila melanogaster: Impact of High- RT Throughput Data."; RL G3 (Bethesda) 5:1721-1736(2015). RN [12] {ECO:0000313|EMBL:AAF52250.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=26109356; DOI=.1534/g3.115.018937; RG FlyBase Consortium; RA Crosby M.A., Gramates L.S., Dos Santos G., Matthews B.B., St Pierre S.E., RA Zhou P., Schroeder A.J., Falls K., Emmert D.B., Russo S.M., Gelbart W.M., RA null; RT "Gene Model Annotations for Drosophila melanogaster: The Rule-Benders."; RL G3 (Bethesda) 5:1737-1749(2015). RN [13] {ECO:0000313|EMBL:AAF52250.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=25589440; RA Hoskins R.A., Carlson J.W., Wan K.H., Park S., Mendez I., Galle S.E., RA Booth B.W., Pfeiffer B.D., George R.A., Svirskas R., Krzywinski M., RA Schein J., Accardo M.C., Damia E., Messina G., Mendez-Lago M., RA de Pablos B., Demakova O.V., Andreyeva E.N., Boldyreva L.V., Marra M., RA Carvalho A.B., Dimitri P., Villasante A., Zhimulev I.F., Rubin G.M., RA Karpen G.H., Celniker S.E.; RT "The Release 6 reference sequence of the Drosophila melanogaster genome."; RL Genome Res. 25:445-458(2015). RN [14] {ECO:0000313|EMBL:AAF52250.1} RP NUCLEOTIDE SEQUENCE. RG Berkeley Drosophila Genome Project; RA Celniker S., Carlson J., Wan K., Pfeiffer B., Frise E., George R., RA Hoskins R., Stapleton M., Pacleb J., Park S., Svirskas R., Smith E., Yu C., RA Rubin G.; RT "Drosophila melanogaster release 4 sequence."; RL Submitted (NOV-2022) to the EMBL/GenBank/DDBJ databases. RN [15] {ECO:0000313|EMBL:AAF52250.1} RP NUCLEOTIDE SEQUENCE. RG FlyBase; RL Submitted (NOV-2022) to the EMBL/GenBank/DDBJ databases. CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123, CC ECO:0000256|PROSITE-ProRule:PRU00201}. CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of CC feature annotation. {ECO:0000256|PROSITE-ProRule:PRU00201}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AE014134; AAF52250.1; -; Genomic_DNA. DR EMBL; BT023769; AAZ41777.1; -; mRNA. DR EMBL; AE014134; AGB92632.1; -; Genomic_DNA. DR RefSeq; NP_001260096.1; NM_001273167.2. DR RefSeq; NP_608927.2; NM_135083.4. DR AlphaFoldDB; Q9VMR2; -. DR SMR; Q9VMR2; -. DR IntAct; Q9VMR2; 16. DR STRING; 7227.FBpp0078726; -. DR PaxDb; 7227-FBpp0078726; -. DR DNASU; 33770; -. DR EnsemblMetazoa; FBtr0079093; FBpp0078726; FBgn0261963. DR EnsemblMetazoa; FBtr0310358; FBpp0302039; FBgn0261963. DR GeneID; 33770; -. DR KEGG; dme:Dmel_CG6634; -. DR UCSC; CG6634-RA; d. melanogaster. DR AGR; FB:FBgn0261963; -. DR CTD; 33770; -. DR FlyBase; FBgn0261963; mid. DR VEuPathDB; VectorBase:FBgn0261963; -. DR eggNOG; KOG3586; Eukaryota. DR GeneTree; ENSGT00940000168440; -. DR HOGENOM; CLU_014430_9_2_1; -. DR InParanoid; Q9VMR2; -. DR OMA; HKSRNES; -. DR OrthoDB; 5323209at2759; -. DR BioGRID-ORCS; 33770; 0 hits in 3 CRISPR screens. DR ChiTaRS; mid; fly. DR GenomeRNAi; 33770; -. DR Proteomes; UP000000803; Chromosome 2L. DR Bgee; FBgn0261963; Expressed in atrium (Drosophila) and 42 other tissues. DR ExpressionAtlas; Q9VMR2; baseline and differential. DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell. DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central. DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; IDA:FlyBase. DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central. DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:FlyBase. DR GO; GO:0001222; F:transcription corepressor binding; IPI:FlyBase. DR GO; GO:0007411; P:axon guidance; IMP:FlyBase. DR GO; GO:0007409; P:axonogenesis; IMP:FlyBase. DR GO; GO:0042684; P:cardioblast cell fate commitment; IMP:FlyBase. DR GO; GO:0001708; P:cell fate specification; IBA:GO_Central. DR GO; GO:0007417; P:central nervous system development; IMP:FlyBase. DR GO; GO:0030381; P:chorion-containing eggshell pattern formation; IMP:FlyBase. DR GO; GO:0035050; P:embryonic heart tube development; IMP:FlyBase. DR GO; GO:0097156; P:fasciculation of motor neuron axon; IMP:FlyBase. DR GO; GO:0007402; P:ganglion mother cell fate determination; IMP:FlyBase. DR GO; GO:0008406; P:gonad development; IMP:FlyBase. DR GO; GO:0007507; P:heart development; IMP:FlyBase. DR GO; GO:0003015; P:heart process; IDA:FlyBase. DR GO; GO:0035223; P:leg disc pattern formation; IGI:FlyBase. DR GO; GO:0010629; P:negative regulation of gene expression; IMP:FlyBase. DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:FlyBase. DR GO; GO:0014019; P:neuroblast development; IMP:FlyBase. DR GO; GO:0014018; P:neuroblast fate specification; IMP:FlyBase. DR GO; GO:0048665; P:neuron fate specification; IMP:FlyBase. DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:FlyBase. DR GO; GO:0051890; P:regulation of cardioblast differentiation; IMP:FlyBase. DR GO; GO:0042659; P:regulation of cell fate specification; IMP:FlyBase. DR GO; GO:0051960; P:regulation of nervous system development; IMP:FlyBase. DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central. DR CDD; cd20193; T-box_TBX20-like; 1. DR Gene3D; 2.60.40.820; Transcription factor, T-box; 1. DR InterPro; IPR008967; p53-like_TF_DNA-bd_sf. DR InterPro; IPR046360; T-box_DNA-bd. DR InterPro; IPR036960; T-box_sf. DR InterPro; IPR001699; TF_T-box. DR InterPro; IPR018186; TF_T-box_CS. DR PANTHER; PTHR11267:SF129; LP04777P-RELATED; 1. DR PANTHER; PTHR11267; T-BOX PROTEIN-RELATED; 1. DR Pfam; PF00907; T-box; 1. DR PRINTS; PR00937; TBOX. DR SMART; SM00425; TBOX; 1. DR SUPFAM; SSF49417; p53-like transcription factors; 1. DR PROSITE; PS01283; TBOX_1; 1. DR PROSITE; PS50252; TBOX_3; 1. PE 2: Evidence at transcript level; KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|PROSITE- KW ProRule:PRU00201}; KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|PROSITE- KW ProRule:PRU00201}; Reference proteome {ECO:0000313|Proteomes:UP000000803}; KW Transcription {ECO:0000256|ARBA:ARBA00023163}; KW Transcription regulation {ECO:0000256|ARBA:ARBA00023015}. FT DOMAIN 190..381 FT /note="T-box" FT /evidence="ECO:0000259|PROSITE:PS50252" FT REGION 90..176 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 512..580 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 90..104 FT /note="Basic residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 105..158 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 530..544 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" SQ SEQUENCE 580 AA; 62943 MW; DBE6E3C7C6DA621C CRC64; MLVGSHPYLC NGVPAAPAAP NASGQTTTTA SKSAAGSATD FSIAAIMARE DASSRESSIR SASPISVEDE VDVDVVDCSD AEEPPTKARR LNHHQHHQHH QHHHNNNNNN NNNNVAHKSR NSGGAVAQTA SAESQLNTSS TSSQGRCSTP PQSPGTEDSE ERLTPEPVQK APKIVGSCNC DDLKPVQCHL ETKELWDRFH DLGTEMIITK TGRRMFPTVR VSFSGPLRQI QPADRYAVLM DIIPMDSKRY RYAYHRSAWL VAGKADPAPP ARLYAHPDSP FSCEALRKQV ISFEKVKLTN NEMDKNGQIV LNSMHRYQPR IHLVRLSHGQ SIPSNPKELQ DLDHKTYVFP ETVFTAVTAY QNQLITKLKI DSNPFAKGFR DSSRLTDFDR DPMEALLLEQ QLRSPLRLFP DPLMQQFAAQ GDPSSMALFE KARQHLQMFG GNSPYAQLMM PQMYQAAAAA AGPPPPPPGL GAFHMFQQQW PQLTAGFLAS ANQQAAAAQA AAQAQAQAQA AAAAMAANRT PPPPPTASTP SSTSSGSPSP DMRPRQYQRF SPYQLPGGQG PPSSAAGSPP AVNASRSPAH //