ID PIWI_DROME Reviewed; 843 AA. AC Q9VKM1; O96674; O96675; DT 19-JUL-2003, integrated into UniProtKB/Swiss-Prot. DT 01-MAY-2000, sequence version 1. DT 15-JUN-2010, entry version 62. DE RecName: Full=Protein piwi; GN Name=piwi; ORFNames=CG6122; OS Drosophila melanogaster (Fruit fly). OC Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; OC Ephydroidea; Drosophilidae; Drosophila; Sophophora. OX NCBI_TaxID=7227; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, DEVELOPMENTAL RP STAGE, AND TISSUE SPECIFICITY. RC STRAIN=Canton-S, and Oregon-R; RX MEDLINE=99069219; PubMed=9851978; RA Cox D.N., Chao A., Baker J., Chang L., Qiao D., Lin H.; RT "A novel class of evolutionarily conserved genes defined by piwi are RT essential for stem cell self-renewal."; RL Genes Dev. 12:3715-3727(1998). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley; RX MEDLINE=20196006; PubMed=10731132; DOI=10.1126/science.287.5461.2185; RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., RA Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., RA Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., RA Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., RA Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., RA Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., RA Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., RA Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., RA Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., RA de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., RA Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., RA Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., RA Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., RA Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., RA Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., RA Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., RA Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., RA Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., RA Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., RA Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., RA Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., RA Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., RA Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., RA Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., RA Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., RA Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., RA Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., RA Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., RA Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., RA Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., RA Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.; RT "The genome sequence of Drosophila melanogaster."; RL Science 287:2185-2195(2000). RN [3] RP GENOME REANNOTATION. RX MEDLINE=22426069; PubMed=12537572; RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A., RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., RA Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., RA Lewis S.E.; RT "Annotation of the Drosophila melanogaster euchromatic genome: a RT systematic review."; RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002). RN [4] RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY. RX PubMed=10631171; RA Cox D.N., Chao A., Lin H.; RT "piwi encodes a nucleoplasmic factor whose activity modulates the RT number and division rate of germline stem cells."; RL Development 127:503-514(2000). CC -!- FUNCTION: Mediates a somatic signaling mechanism required for the CC maintenance of germline stem cells to produce and maintain a CC daughter germline stem cell. It is not essential for the further CC differentiation of the committed daughter cell. Acts cell CC autonomously to promote germline stem cell division. CC -!- SUBCELLULAR LOCATION: Nucleus, nucleoplasm. Note=Nurse cells, CC follicle cells and oocytes in developing egg chambers. CC -!- TISSUE SPECIFICITY: Expressed somatically in the ovariole terminal CC filament and epithelial sheath cells. Expressed in the germarium CC at low levels during oogenesis stages 1-6, at a lower level during CC stages 7-9, strongly at stage 10 and eventually accumulating in CC early embryos. CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically. CC -!- SIMILARITY: Belongs to the argonaute family. Piwi subfamily. CC -!- SIMILARITY: Contains 1 PAZ domain. CC -!- SIMILARITY: Contains 1 Piwi domain. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AF104354; AAD08704.1; -; mRNA. DR EMBL; AF104355; AAD08705.1; -; Genomic_DNA. DR EMBL; AE014134; AAF53043.1; -; Genomic_DNA. DR RefSeq; NP_476875.1; -. DR UniGene; Dm.19816; -. DR STRING; Q9VKM1; -. DR PRIDE; Q9VKM1; -. DR EnsemblMetazoa; FBtr0080166; FBpp0079755; FBgn0004872. DR GeneID; 34521; -. DR KEGG; dme:Dmel_CG6122; -. DR NMPDR; fig|7227.3.peg.1939; -. DR CTD; 34521; -. DR FlyBase; FBgn0004872; piwi. DR eggNOG; inNOG04071; -. DR InParanoid; Q9VKM1; -. DR OMA; NIRIRDH; -. DR OrthoDB; EOG918BTP; -. DR PhylomeDB; Q9VKM1; -. DR BioCyc; DMEL-XXX-02:DMEL-XXX-02-008498-MONOMER; -. DR NextBio; 788889; -. DR ArrayExpress; Q9VKM1; -. DR Bgee; Q9VKM1; -. DR GermOnline; CG6122; Drosophila melanogaster. DR GO; GO:0010369; C:chromocenter; IDA:FlyBase. DR GO; GO:0000791; C:euchromatin; IDA:FlyBase. DR GO; GO:0005654; C:nucleoplasm; IDA:UniProtKB. DR GO; GO:0005700; C:polytene chromosome; IDA:FlyBase. DR GO; GO:0031933; C:telomeric heterochromatin; IDA:FlyBase. DR GO; GO:0005515; F:protein binding; IPI:FlyBase. DR GO; GO:0003723; F:RNA binding; IDA:FlyBase. DR GO; GO:0001709; P:cell fate determination; TAS:FlyBase. DR GO; GO:0006342; P:chromatin silencing; IMP:FlyBase. DR GO; GO:0048132; P:female germ-line stem cell division; IMP:UniProtKB. DR GO; GO:0030718; P:germ-line stem cell maintenance; IDA:UniProtKB. DR GO; GO:0048133; P:male germ-line stem cell division; IMP:UniProtKB. DR GO; GO:0000335; P:negative regulation of transposition, DNA-m...; IMP:FlyBase. DR GO; GO:0007279; P:pole cell formation; IMP:FlyBase. DR GO; GO:0016246; P:RNA interference; TAS:FlyBase. DR InterPro; IPR003100; PAZ. DR InterPro; IPR003165; Piwi. DR InterPro; IPR012337; PolynucTfrase_RNaseH_fold. DR Pfam; PF02170; PAZ; 1. DR Pfam; PF02171; Piwi; 1. DR SUPFAM; SSF53098; RNaseH_fold; 1. DR PROSITE; PS50821; PAZ; 1. DR PROSITE; PS50822; PIWI; 1. PE 2: Evidence at transcript level; KW Complete proteome; Developmental protein; Nucleus; KW RNA-mediated gene silencing. FT CHAIN 1 843 Protein piwi. FT /FTId=PRO_0000194067. FT DOMAIN 262 372 PAZ. FT DOMAIN 538 829 Piwi. FT CONFLICT 67 67 T -> P (in Ref. 1; AAD08704). FT CONFLICT 84 84 A -> V (in Ref. 1; AAD08704). FT CONFLICT 122 124 EPS -> VPT (in Ref. 1; AAD08704). FT CONFLICT 576 577 NR -> KPY (in Ref. 1; AAD08705). SQ SEQUENCE 843 AA; 97177 MW; 9B4A95E688E9D9B7 CRC64; MADDQGRGRR RPLNEDDSST SRGSGDGPRV KVFRGSSSGD PRADPRIEAS RERRALEEAP RREGGPTERK PWGDQYDYLN TRPAELVSKK GTDGVPVMLQ TNFFRLKTKP EWRIVHYHVE FEPSIENPRV RMGVLSNHAN LLGSGYLFDG LQLFTTRKFE QEITVLSGKS KLDIEYKISI KFVGFISCAE PRFLQVLNLI LRRSMKGLNL ELVGRNLFDP RAKIEIREFK MELWPGYETS IRQHEKDILL GTEITHKVMR TETIYDIMRR CSHNPARHQD EVRVNVLDLI VLTDYNNRTY RINDVDFGQT PKSTFSCKGR DISFVEYYLT KYNIRIRDHN QPLLISKNRD KALKTNASEL VVLIPELCRV TGLNAEMRSN FQLMRAMSSY TRMNPKQRTD RLRAFNHRLQ NTPESVKVLR DWNMELDKNV TEVQGRIIGQ QNIVFHNGKV PAGENADWQR HFRDQRMLTT PSDGLDRWAV IAPQRNSHEL RTLLDSLYRA ASGMGLRIRS PQEFIIYDDR TGTYVRAMDD CVRSDPKLIL CLVPNDNAER YSSIKKRGYV DRAVPTQVVT LKTTKNRSLM SIATKIAIQL NCKLGYTPWM IELPLSGLMT IGFDIAKSTR DRKRAYGALI ASMDLQQNST YFSTVTECSA FDVLANTLWP MIAKALRQYQ HEHRKLPSRI VFYRDGVSSG SLKQLFEFEV KDIIEKLKTE YARVQLSPPQ LAYIVVTRSM NTRFFLNGQN PPPGTIVDDV ITLPERYDFY LVSQQVRQGT VSPTSYNVLY SSMGLSPEKM QKLTYKMCHL YYNWSGTTRV PAVCQYAKKL ATLVGTNLHS IPQNALEKKF YYL //