ID BERO_DROME Reviewed; 148 AA. AC Q9VII1; M9NEV7; Q8ST39; DT 27-MAR-2024, integrated into UniProtKB/Swiss-Prot. DT 01-MAY-2000, sequence version 1. DT 02-OCT-2024, entry version 161. DE RecName: Full=UPAR/Ly6 domain-containing protein bero {ECO:0000305}; DE AltName: Full=Protein belly roll {ECO:0000303|PubMed:37309249}; DE Flags: Precursor; GN Name=bero {ECO:0000312|FlyBase:FBgn0032897}; GN ORFNames=CG9336 {ECO:0000312|FlyBase:FBgn0032897}; OS Drosophila melanogaster (Fruit fly). OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; OC Drosophilidae; Drosophila; Sophophora. OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803}; RN [1] {ECO:0000312|Proteomes:UP000000803} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803}; RX PubMed=10731132; DOI=10.1126/science.287.5461.2185; RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C., RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., RA Venter J.C.; RT "The genome sequence of Drosophila melanogaster."; RL Science 287:2185-2195(2000). RN [2] {ECO:0000312|Proteomes:UP000000803} RP GENOME REANNOTATION. RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803}; RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083; RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A., RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.; RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic RT review."; RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002). RN [3] {ECO:0000312|EMBL:AAL49243.1, ECO:0000312|EMBL:AAL68332.1} RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). RC STRAIN=Berkeley {ECO:0000312|EMBL:AAL49243.1, ECO:0000312|EMBL:AAL68332.1}; RC TISSUE=Embryo {ECO:0000312|EMBL:AAL49243.1, ECO:0000312|EMBL:AAL68332.1}; RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080; RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., RA Celniker S.E.; RT "A Drosophila full-length cDNA resource."; RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002). RN [4] {ECO:0000312|EMBL:ACD81668.1} RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). RC STRAIN=Berkeley {ECO:0000312|EMBL:ACD81668.1}; RA Carlson J., Booth B., Frise E., Park S., Wan K., Yu C., Celniker S.; RL Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases. RN [5] {ECO:0000305} RP SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE. RX PubMed=28961967; DOI=10.1093/molbev/msx237; RA Baudouin-Gonzalez L., Santos M.A., Tempesta C., Sucena E., Roch F., RA Tanaka K.; RT "Diverse Cis-Regulatory Mechanisms Contribute to Expression Evolution of RT Tandem Gene Duplicates."; RL Mol. Biol. Evol. 34:3132-3147(2017). RN [6] {ECO:0000305} RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION RP PHENOTYPE. RX PubMed=37309249; DOI=10.7554/elife.83856; RA Li K., Tsukasa Y., Kurio M., Maeta K., Tsumadori A., Baba S., Nishimura R., RA Murakami A., Onodera K., Morimoto T., Uemura T., Usui T.; RT "Belly roll, a GPI-anchored Ly6 protein, regulates Drosophila melanogaster RT escape behaviors by modulating the excitability of nociceptive peptidergic RT interneurons."; RL Elife 12:0-0(2023). CC -!- FUNCTION: Necessary for the maintenance of persistent fluctuating CC activities and suppression of acute evoked activities in abdominal CC leucokinin-producing (ABLK) neurons to negatively regulate neuron CC excitability involved in nociceptive (perception of pain) behavioral CC responses. {ECO:0000269|PubMed:37309249}. CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:28961967, CC ECO:0000269|PubMed:37309249}; Lipid-anchor, GPI-anchor {ECO:0000255}. CC Membrane {ECO:0000269|PubMed:37309249}; Lipid-anchor, GPI-anchor CC {ECO:0000255}. Perikaryon {ECO:0000269|PubMed:37309249}. Cell CC projection, neuron projection {ECO:0000269|PubMed:37309249}. CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=2; CC Name=A {ECO:0000312|FlyBase:FBgn0032897}; CC IsoId=Q9VII1-1; Sequence=Displayed; CC Name=B {ECO:0000312|FlyBase:FBgn0032897}; CC IsoId=Q9VII1-2; Sequence=VSP_062238; CC -!- DEVELOPMENTAL STAGE: Expressed in insulin-producing cells and a subset CC of eclosion hormone-producing (EH) neurons of the third-instar larval CC brain (at protein level) (PubMed:37309249). Expressed in all seven CC pairs of abdominal leucokinin-producing (ABLK) neurons in the lateral CC region of abdominal ganglions of the third-instar larval ventral nerve CC cord, but not in other leucokinin-producing neurons (at protein level) CC (PubMed:37309249). Expressed in third-instar larval non-neuronal CC tissue, including midline glial cells (at protein level) CC (PubMed:37309249). Expressed in the embryonic glia of the peripheral CC nervous system, the wrapping glia of the larval eye disk and the CC midline glia throughout development (at protein level) CC (PubMed:28961967). Expressed in the surface glia of the embryonic CC central nervous system (at protein level) (PubMed:28961967). In the CC embryo, expressed in Bolwig's organ, heart and anal plate (at protein CC level) (PubMed:28961967). Expressed in embryonic hindgut epithelial CC boundary cells, nephrocytic Garland cells and Capa-producing neurons of CC the central nervous system (at protein level) (PubMed:28961967). CC {ECO:0000269|PubMed:28961967, ECO:0000269|PubMed:37309249}. CC -!- DISRUPTION PHENOTYPE: Enhanced rolling escape behavior in response to CC nociception (perception of pain). {ECO:0000269|PubMed:37309249}. CC -!- MISCELLANEOUS: Larvae display an instinctive escape response to CC nociception (the sensing of noxious stimuli such as harsh mechanical CC attacks) consisting of an abrupt bending of the body followed by a CC corkscrew-like roll reminiscent of an athletics high-jump technique CC referred to as the belly roll. {ECO:0000303|PubMed:37309249}. CC -!- SIMILARITY: Belongs to the quiver family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AE014134; AAF53938.1; -; Genomic_DNA. DR EMBL; AE014134; AFH03788.1; -; Genomic_DNA. DR EMBL; AY071621; AAL49243.1; -; mRNA. DR EMBL; AY075525; AAL68332.1; -; mRNA. DR EMBL; BT032654; ACD81668.1; -; mRNA. DR RefSeq; NP_001246114.1; NM_001259185.2. [Q9VII1-2] DR RefSeq; NP_610069.2; NM_136225.4. [Q9VII1-1] DR AlphaFoldDB; Q9VII1; -. DR IntAct; Q9VII1; 18. DR STRING; 7227.FBpp0080956; -. DR PaxDb; 7227-FBpp0080956; -. DR DNASU; 35355; -. DR EnsemblMetazoa; FBtr0081427; FBpp0080956; FBgn0032897. [Q9VII1-1] DR EnsemblMetazoa; FBtr0307498; FBpp0298833; FBgn0032897. [Q9VII1-2] DR GeneID; 35355; -. DR KEGG; dme:Dmel_CG9336; -. DR UCSC; CG9336-RA; d. melanogaster. [Q9VII1-1] DR AGR; FB:FBgn0032897; -. DR CTD; 35355; -. DR FlyBase; FBgn0032897; bero. DR VEuPathDB; VectorBase:FBgn0032897; -. DR eggNOG; ENOG502T7Z1; Eukaryota. DR GeneTree; ENSGT00540000073450; -. DR HOGENOM; CLU_119218_0_0_1; -. DR InParanoid; Q9VII1; -. DR OMA; TTPKCGL; -. DR OrthoDB; 3378720at2759; -. DR BioGRID-ORCS; 35355; 0 hits in 1 CRISPR screen. DR GenomeRNAi; 35355; -. DR Proteomes; UP000000803; Chromosome 2L. DR Bgee; FBgn0032897; Expressed in oviduct (Drosophila) and 53 other tissues. DR ExpressionAtlas; Q9VII1; baseline and differential. DR GO; GO:0016020; C:membrane; IDA:FlyBase. DR GO; GO:0043005; C:neuron projection; IEA:UniProtKB-SubCell. DR GO; GO:0043204; C:perikaryon; IEA:UniProtKB-SubCell. DR GO; GO:0005886; C:plasma membrane; HDA:FlyBase. DR GO; GO:0098552; C:side of membrane; IEA:UniProtKB-KW. DR GO; GO:1904057; P:negative regulation of sensory perception of pain; IMP:FlyBase. DR GO; GO:0032222; P:regulation of synaptic transmission, cholinergic; IEA:InterPro. DR GO; GO:0030431; P:sleep; IEA:InterPro. DR InterPro; IPR031424; QVR-like. DR InterPro; IPR050975; Sleep_regulator. DR PANTHER; PTHR33562; ATILLA, ISOFORM B-RELATED-RELATED; 1. DR PANTHER; PTHR33562:SF2; PROTEIN QUIVER; 1. DR Pfam; PF17064; QVR; 1. DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1. PE 1: Evidence at protein level; KW Alternative splicing; Cell membrane; Cell projection; Disulfide bond; KW Glycoprotein; GPI-anchor; Lipoprotein; Membrane; Reference proteome; KW Signal; Transmembrane; Transmembrane helix. FT SIGNAL 1..23 FT /evidence="ECO:0000255" FT CHAIN 24..125 FT /note="UPAR/Ly6 domain-containing protein bero" FT /evidence="ECO:0000255" FT /id="PRO_5015100315" FT PROPEP 126..148 FT /note="Removed in mature form" FT /evidence="ECO:0000255" FT /id="PRO_0000459695" FT TRANSMEM 128..148 FT /note="Helical" FT /evidence="ECO:0000255" FT LIPID 125 FT /note="GPI-anchor amidated asparagine" FT /evidence="ECO:0000255" FT CARBOHYD 68 FT /note="N-linked (GlcNAc...) asparagine" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498" FT CARBOHYD 125 FT /note="N-linked (GlcNAc...) asparagine" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498" FT DISULFID 26..72 FT /evidence="ECO:0000305|PubMed:28961967, FT ECO:0000305|PubMed:37309249" FT DISULFID 29..37 FT /evidence="ECO:0000305|PubMed:28961967, FT ECO:0000305|PubMed:37309249" FT DISULFID 51..90 FT /evidence="ECO:0000305|PubMed:28961967, FT ECO:0000305|PubMed:37309249" FT DISULFID 102..116 FT /evidence="ECO:0000305|PubMed:28961967, FT ECO:0000305|PubMed:37309249" FT DISULFID 119..124 FT /evidence="ECO:0000305|PubMed:28961967, FT ECO:0000305|PubMed:37309249" FT VAR_SEQ 1..33 FT /note="Missing (in isoform B)" FT /id="VSP_062238" FT CONFLICT 118 FT /note="V -> A (in Ref. 3; AAL49243/AAL68332)" FT /evidence="ECO:0000305" SQ SEQUENCE 148 AA; 15887 MW; A8CCD29DC0B7D148 CRC64; MVSALKCSLA VAVMISLACS AYAIKCYQCE SLTMPKCGLK FEADETLLLD CSRIGPPRYL QNFFPLRNAT GCMKKTLESV AGHPQIVRSC YFGDINNIQA GCQSDPSMPF VKQLGCDVCT KDECNGSSSL APIAGAILLF FGVARLLA //