ID Q9VH01 PRELIMINARY; PRT; 4904 AA. AC Q9VH01; DT 01-MAY-2000 (TrEMBLrel. 13, Created) DT 01-MAR-2001 (TrEMBLrel. 16, Last sequence update) DT 01-DEC-2001 (TrEMBLrel. 19, Last annotation update) DE CG6303 PROTEIN. GN BRUCE OR CG6303. OS Drosophila melanogaster (Fruit fly). OC Eukaryota; Metazoa; Arthropoda; Tracheata; Hexapoda; Insecta; OC Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; OC Ephydroidea; Drosophilidae; Drosophila. OX NCBI_TaxID=7227; RN [1] RP SEQUENCE FROM N.A. RC STRAIN=BERKELEY; RX MEDLINE=20196006; PubMed=10731132; RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., RA Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., RA Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., RA Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., RA Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., RA Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., RA Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., RA Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., RA Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., RA de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., RA Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., RA Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., RA Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., RA Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., RA Harris N.L., Harvey D., Heiman T.J., Hernandez J.R., Houck J., RA Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., RA Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., RA Kimmel B.E., Kodira C.D., Kraft C., Kravitz S., Kulp D., Lai Z., RA Lasko P., Lei Y., Levitsky A.A., Li J., Li Z., Liang Y., Lin X., RA Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., RA Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., RA Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., RA Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., RA Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., RA Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., RA Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T., RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., RA Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., RA Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., RA Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., RA Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., RA Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S., Zhu X., Smith H.O., RA Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.; RT "The genome sequence of Drosophila melanogaster."; RL Science 287:2185-2195(2000). CC -!- FUNCTION: CATALYZES THE COVALENT ATTACHMENT OF UBIQUITIN TO OTHER CC PROTEINS (BY SIMILARITY). CC -!- CATALYTIC ACTIVITY: ATP + UBIQUITIN + PROTEIN LYSINE = AMP + CC PYROPHOSPHATE + PROTEIN N-UBIQUITYLLYSINE. CC -!- PATHWAY: SECOND STEP IN UBIQUITIN CONJUGATION. CC -!- MISCELLANEOUS: A CYSTEINE RESIDUE IS REQUIRED FOR UBIQUITIN- CC THIOLESTER FORMATION (BY SIMILARITY). CC -!- SIMILARITY: BELONGS TO THE UBIQUITIN-CONJUGATING ENZYME FAMILY. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AE003686; AAF54520.2; -. DR HSSP; Q13490; 1QBH. DR FlyBase; FBgn0037808; Bruce. DR InterPro; IPR001370; BIR. DR InterPro; IPR000608; UBQ_conjugat. DR Pfam; PF00653; BIR; 1. DR Pfam; PF00179; UQ_con; 1. DR SMART; SM00238; BIR; 1. DR SMART; SM00212; UBCc; 1. DR PROSITE; PS50143; BIR_REPEAT_2; 1. DR PROSITE; PS50127; UBIQUITIN_CONJUGAT_2; 1. KW Ligase; Ubiquitin conjugation. SQ SEQUENCE 4904 AA; 542137 MW; 626D616B6FE1A4CF CRC64; MATEQHHQQR VLKQDGYLDL GDCNSAPSRP FEMRNVVYHP QLNVLLLFDN GSTVKVLDAN SGVILQTYHL SSNGDSIVHG RYMPLQDKIL FWDGHNLGLR GDYNGVLLLD TILQAPIGQN DDYIKLEIIL SEAIIFQNCI TELESEGLEC PCDISNELTQ KINQAQLKAK KGIKAQRWNT ICLEVPYSSL KLVSNNMVIL LKRLERHIPV LAIASAINER LTDMMMGSRV PDFGWNFSNF QRVLMHSEAV RRQTFEKWPH MDYKWALPDQ MAQAGFYHQP SSSGEDRAMC FTCSVCLVCW EKTDEPWSEH ERHSPLCPFV KGEYTQNVPL SITYATNPAL PAPGLGFDII SNSDYANVLC TSCSQTGELS VWSIERHLKL MHTFHVPTLL NYIFEESFEW ARVTAICVLP NARARTKVNY VYSAANYGGS GSSNGAGCNS GLNAVSGGKF GSVNAQHITS TSTLRAGVVG SKIVLGVSVR QSSGELALRM VVLNIVEVDR NSESSTSVSN DSGSVSSGGG QLMKSSSPMV NVSAENNATV TQVNLESKDD DNKAMTPYEK FADGFDSNGF VSALMTYNKN NGVSQNTDDA SALYDFDLCT LSQILDHNLE EPLSLMVGSS NTVNVSNIGM GNEINKLVVD VEISHQNNNN NNNNNNEASS LGGNSSNNNN NIMINGGTNS SIDKMTAIEE IDCVVESWTS VKRMNALDGA SSGVNGVNHM DQEVIEIAEI LPTTPKCNQL LVKLLRTKAP EAEGSKIIMA TVAKEEEEED EEQRTDESME TDNNAEGNVA AQLLVFDYDD TEISNDYTLA MTFSRAKFPK QLCILPHFNS SPEHKDAGSI CVVCADGTVE IYSLADFQGT MVIEEEGEHF ESVVYCRNLD RLCCCSRQGG LLFYSLSDGE NDSGDELLEM DDDCSTTLTQ AADVSVTDGG RQLARTDLLG KTLLANESIC MDAGSEAAAI PNSSSAAQGA HVQTSPSASS APSTNANLLA YKTADLTLED LKVFYALTQF DDKLTVYSAE VPSCWNDIGQ VQNQRKQSQN MRHGGDERDF TRTWRLHNDA TTWDEHIIEL NLAQPVSLGH IDLKFTVYQQ CSNPAAIQVT LLKQNNNGFG YRMKGPHSSY KPNVIDDNID LSYIANENPV LSEEYLLAHN AEVLAGPIEL SSCIDLCDQG GTATLTSPKL FKTRTRNFLL HIKTVSDPSK DGQNKTRDSP TRHKRPHHVE GFVERNKVSR NPFNVAANII SVVKTAVLEV FPAAKNACVK IIQVNKPKTQ DNEKVFVAKI IKNKTQSQSA AVAPSVGRRL EQLPSREGCD WLHEISISVR AVKSHSRISN GRMQRIAMLE SNVLLVQLLR IASDPASSSL AKNLSLDILN WICFIRLNRF RSPKSERLKD QINKQTAANI QDLLAQQFEC ISLAEKHIEQ LLRNCVIYSE RSTAHKCVKL LIMLTEGATN LPAEVQDHTT LDYSFKAAIA NTFPELPRAQ CGSVVRWYMM LTCATSTAES HNSISEQAIS LLKEIAAEID KRWDPHCALL CTRFGLYGYP FEPEIFDFDF PNVSRQGPGS SVANVIRSSP TILPVTGLDI KRLSSIDGVD FRTFPYLIRG KSISNQLRGL LEVVQLHFNC VQTSEATRID NIDSVGGNTG ANVIIEDVMM MPKQVVAKEK GEEMLNDLVN DVECLVEEMK VDENEKQEKV DYDAFGLVNG FKDKTLSSLN SVKQTLKEEV KKEKADIMDK LTIAKFLPKL SLLESYMQKK INEHISFDDF GKLDNWDGNT TPPFNLEVEA PIVCSEATSE SNMSQQATSA GGNILDDAAD SEKITESLNA NPCQPIAWHK LITPPPKQMI VIDRMHSGAR RFVVLDFGQH ILLTDLVIPS CDELTSLNID IWCFDEETDC TRLVQVNDIQ TKTLVLSDIQ PAPICRYLKL TIIGRIGMSA TKCKVPLGRF FGHPVVLEHD GYGDQLMKYI KHPTHSLQSQ IKSLNALYED VHCRYSLASS KLMDLLAPMR NSELSNVAHL QAFINKQREE ELSGLENNNK VVSLYDECML LQYQINVIRN VVLRLERALS PNSPTTTLLP GQSIPLTEDI LRAASRDKLR VLSLSLVEVL LHFSIEYGIK NILPVHQRFN AATAKALFNA LVVYGDAQLQ LATCSLLVRM CCFQPWWVID AVLKTLVKLL APISPHYKFQ SEQPSTSANA ADRNAAMGTT SDLQLITWLL LFLSVCLDDN ERKDKSAARW DFMSCEGDFT KARPSATPNI KQLRCFKKRI IQQNKYLAQP YADWGKKIYM IQNEHPGIFM ELSTKSKGAG PTKSTSTGGK INMGSSGLCV GGPSSSSGNT SKSAASSSSP KNQAVPGTDG ERDSNFDKGL KSLRMDNIKV VIRGLFALLL EMDFDCNMDQ FLLTCKVIAR LVAACRPSVQ LCKIVTIAQL QQLVRLSVWN DQQHPWAVHA ITCLLQDLLD ADKQFRDNDV TPTNDTPRTM EAAIQETRDL FSDYTSVKYN YLPSLIECDD TEFDEMLNDI DMIERTKPVT KKESNALSKN TFNFFCKSIS IALDSRLDVG LELHVETQLR RLTNRTSLDL YSSLPQVLTN EFVSPPPEAA PWPEYLTQLW SGTEYNGDNT YVMFKRVFDS ILAELHLEDT WVHLEQVLQM WLTLNCELSD KPYTSGITHS DVPKIPFGAN AVQGLLLALA WHSDIKLRTW CLGFQCLFLA CNSLPIGDDA DCTRINEAIV NDENFEKVLL RFFSGYGMSS SIITNRCAGP TICKHLHELL LWLHSKSETS GIPCKRRLKD ILLHVVLQLV QPGGSISNQQ GPIDAQNQLV RDLLMMPNDK GDLNIALKIT ESVSFLVYNN ISNGERLQCQ RGNENSPVGN NFSNLFANVL GSENPNKQNA AICDNSLIIN LLKLSSHMEV TIPEEEELSN QSQTDETKAE QLNTEGHRSK VPCIADWVLR HFPTMKRLFG TLSQCSTNTF TMMSSGCFFS PKSNMILDDP QTIADAVFSL MITLNDKASH PRLVVVPICR YLEETTIPRN ATLPRLPHSE PFLWYISKVL EVPAAVQTFT QLGGIQIICH SLVRLNKTII NMQPGLISLI MQHLTRNTRF KRNNHVAASS ACKKTTTGTG TTCPTSAQPP RSAAQYDGLI NFAPFSHIVS ENDATQSAEV LISNPNNSHR RPRSPAWTYM FYPNETHVDL TITLPTAILL KEVQLVPHTT SLASCPSAVA LELSRDYGLG SIPVGAPMAT TGLTCIRLKL AKAEVATSIV LRLYKPKDCG NVGLVQIAVL GQTIFGNRMH GLRSPSPFID SLATCSSPVQ TAVLAAMDAD SMLEDDAFAK TSIGWVRILS RCFHVAALQP DSKLADLIAA YPAAYPGFLE ACCSLLNIMP MIPSLAQQHL ETILLKLSAY NHEISLQLLR TLLWHTTPQV FKLSSDSVCD LIYEVVTGST EHLLDKLRVL IDWVMQLHDN YSKQARCNNP QSGFVKVIAS ILWKVHTQQQ LPELPLLISS SLFETCFEWV SSLEHDEPLK SSFDSLLCAL CFIKPELFNQ LLVKLGVKLE PPARGQTDDS KVQSGSAWFR REGSENLSVL LQRPTFLKTL ALACQSPAAV YQMLDSGLPK LLAHAIYEYC MHLMPGSEPV ASASTVPEDE PLANNQAAAA ADDEDSSLTD FDKAEANVVY NTPLLSSSNV PKVLDFFAEC CAEGPMRDWL GTMQGSVFWK PLLQLLCNTH AAQFSKTLPK EQSFIRLERA TINFFTRVSA CHPGNQENLT SLLIGAIICK PLRFSHGVRP TISGFTRQMV LQLLLENEHI TVSVRSQQPL QRRDTLSAVL GGISSGASTS LPVAAVNNHP ARRSSAHHML FTVTTSTTCQ EIMQNCVSDQ RAGETSASSS GVSSATAKSS ESKMEKSSFL NFNNVEAGSM EFLTVGAAVA AKDKRIKDAK NQAAMNKDKE TQTSSTNLAS NIFNVDEFLL QSANAVNGNQ LVIPEYSDIV LAGDATISQV LASLQNAGHS LSTPCISFDL VLQQRSDDTS EAKKTKAACT EPLPSPLQRF SSSGGLSLLA HYLPTVYPEH SGRKTTLVFS EKEKSPPLSD WVKLEPNDEI YEDLEDTLCE PAAKLATVSS VPQHSLAAFG LFLRLPAYSD VLLRDKMRAQ CLLRLVLGVT GDGDGSTLKE WSINSLTNPL SVPDDIYSLS LAPSLPTLPF EVFRQLLDSV PLSTDDGVLL RRVVIEVGAM HLVLNCLGIF SHQSHNNRIG APISEPSPSG TSSGSGTIGN GSGNGSVSGT KTNAPEEATP SATSDDKSHM YWAKGTGFGT GSTTQSWNVE QALLRQKSEE EHVTVLLLVL ASYINPGDCI PSEMCGEDIL AYHDVRDVTG ELPPIFQTLL QQSCLIPALS SYLRNDSVLD ITRHIPLYRA ILKLLRALSL SKMLVSLLQP SASGSGDSTP PIVELLTSMK TCVDTYAKRL KVNKKSNIKG QTHQLTFNID DGDDEGLALL IPDIHETSVL VQKTTDADAL INMLHTNEDG SAQLEPQSKS IEQRYLELMK KRQFDTFDMI VESDNNSFRF VVSHHFEKMV RLAGDRYHPS RVKRLAQEAV TLSTSLPLSF SSSVFVRCDT DRLDIMKVLI TGPADTPYAN GCFEFDVFFP PDYPNQPMLI NLETTGRHSV RFNPNLYNDG KVCLSVLNTW HGRPEEKWNA QTSSFLQVLV SIQSLILVPE PYFNEPGFER SRGSPSGTNS SREYNSNIYQ ACVRWAMLEQ IRSPSQCFKD VIHKHFWLKR EEICAQIEGW IEELGKPQYT ERASRTISFN SMVLRRHYRH LREELSKLKP PRGLEDLDAP FNPVATLPPM DVSAVAPSLA ATNTVQAQPD DAIVTDITLL GENADECEAD GDAEGDGEVD DGFKGFPQEE GLLTGEEEVV EILSDTENES SMWQ //