ID Q9VDR7_DROME Unreviewed; 829 AA. AC Q9VDR7; DT 01-MAY-2000, integrated into UniProtKB/TrEMBL. DT 01-OCT-2002, sequence version 2. DT 29-MAY-2024, entry version 150. DE SubName: Full=Miranda, isoform A {ECO:0000313|EMBL:AAF55723.2}; GN Name=mira {ECO:0000313|EMBL:AAF55723.2, GN ECO:0000313|FlyBase:FBgn0021776}; GN Synonyms=Dmel\CG12249 {ECO:0000313|EMBL:AAF55723.2}, MIR GN {ECO:0000313|EMBL:AAF55723.2}, Mir {ECO:0000313|EMBL:AAF55723.2}, mir GN {ECO:0000313|EMBL:AAF55723.2}, MIRA {ECO:0000313|EMBL:AAF55723.2}, GN Mira {ECO:0000313|EMBL:AAF55723.2}, Miranda GN {ECO:0000313|EMBL:AAF55723.2}; GN ORFNames=CG12249 {ECO:0000313|EMBL:AAF55723.2, GN ECO:0000313|FlyBase:FBgn0021776}, Dmel_CG12249 GN {ECO:0000313|EMBL:AAF55723.2}; OS Drosophila melanogaster (Fruit fly). OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; OC Drosophilidae; Drosophila; Sophophora. OX NCBI_TaxID=7227 {ECO:0000313|EMBL:AAF55723.2, ECO:0000313|Proteomes:UP000000803}; RN [1] {ECO:0000313|EMBL:AAF55723.2, ECO:0000313|Proteomes:UP000000803} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803}; RX PubMed=10731132; DOI=10.1126/science.287.5461.2185; RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C., RA Rogers Y.H., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., RA Baxter E.G., Helt G., Nelson C.R., Gabor G.L., Abril J.F., Agbayani A., RA An H.J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., RA Harris N.L., Harvey D., Heiman T.J., Hernandez J.R., Houck J., Hostin D., RA Houston K.A., Howland T.J., Wei M.H., Ibegwam C., Jalali M., Kalush F., RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., RA Kraft C., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., RA Li J., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., RA Reese M.G., Reinert K., Remington K., Saunders R.D., Scheeler F., Shen H., RA Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T., Spier E., RA Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., RA Turner R., Venter E., Wang A.H., Wang X., Wang Z.Y., Wassarman D.A., RA Weinstock G.M., Weissenbach J., Williams S.M., WoodageT, Worley K.C., RA Wu D., Yang S., Yao Q.A., Ye J., Yeh R.F., Zaveri J.S., Zhan M., Zhang G., RA Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S., RA Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.; RT "The genome sequence of Drosophila melanogaster."; RL Science 287:2185-2195(2000). RN [2] {ECO:0000313|EMBL:AAF55723.2, ECO:0000313|Proteomes:UP000000803} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803}; RX PubMed=12537568; RA Celniker S.E., Wheeler D.A., Kronmiller B., Carlson J.W., Halpern A., RA Patel S., Adams M., Champe M., Dugan S.P., Frise E., Hodgson A., RA George R.A., Hoskins R.A., Laverty T., Muzny D.M., Nelson C.R., RA Pacleb J.M., Park S., Pfeiffer B.D., Richards S., Sodergren E.J., RA Svirskas R., Tabor P.E., Wan K., Stapleton M., Sutton G.G., Venter C., RA Weinstock G., Scherer S.E., Myers E.W., Gibbs R.A., Rubin G.M.; RT "Finishing a whole-genome shotgun: release 3 of the Drosophila melanogaster RT euchromatic genome sequence."; RL Genome Biol. 3:RESEARCH0079-RESEARCH0079(2002). RN [3] {ECO:0000313|EMBL:AAF55723.2, ECO:0000313|Proteomes:UP000000803} RP GENOME REANNOTATION. RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803}; RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083; RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A., RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.; RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic RT review."; RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002). RN [4] {ECO:0000313|EMBL:AAF55723.2, ECO:0000313|Proteomes:UP000000803} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803}; RX PubMed=12537573; RA Kaminker J.S., Bergman C.M., Kronmiller B., Carlson J., Svirskas R., RA Patel S., Frise E., Wheeler D.A., Lewis S.E., Rubin G.M., Ashburner M., RA Celniker S.E.; RT "The transposable elements of the Drosophila melanogaster euchromatin: a RT genomics perspective."; RL Genome Biol. 3:RESEARCH0084.1-RESEARCH0084.20(2002). RN [5] {ECO:0000313|EMBL:AAF55723.2, ECO:0000313|Proteomes:UP000000803} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803}; RX PubMed=12537574; RA Hoskins R.A., Smith C.D., Carlson J.W., Carvalho A.B., Halpern A., RA Kaminker J.S., Kennedy C., Mungall C.J., Sullivan B.A., Sutton G.G., RA Yasuhara J.C., Wakimoto B.T., Myers E.W., Celniker S.E., Rubin G.M., RA Karpen G.H.; RT "Heterochromatic sequences in a Drosophila whole-genome shotgun assembly."; RL Genome Biol. 3:RESEARCH0085-RESEARCH0085(2002). RN [6] {ECO:0000313|EMBL:AAF55723.2, ECO:0000313|Proteomes:UP000000803} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803}; RX PubMed=16110336; DOI=10.1371/journal.pcbi.0010022; RA Quesneville H., Bergman C.M., Andrieu O., Autard D., Nouaud D., RA Ashburner M., Anxolabehere D.; RT "Combined evidence annotation of transposable elements in genome RT sequences."; RL PLoS Comput. Biol. 1:166-175(2005). RN [7] {ECO:0000313|EMBL:AAF55723.2, ECO:0000313|Proteomes:UP000000803} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803}; RX PubMed=17569856; DOI=10.1126/science.1139815; RA Smith C.D., Shu S., Mungall C.J., Karpen G.H.; RT "The Release 5.1 annotation of Drosophila melanogaster heterochromatin."; RL Science 316:1586-1591(2007). RN [8] {ECO:0000313|EMBL:AAF55723.2, ECO:0000313|Proteomes:UP000000803} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803}; RX PubMed=17569867; DOI=10.1126/science.1139816; RA Hoskins R.A., Carlson J.W., Kennedy C., Acevedo D., Evans-Holm M., RA Frise E., Wan K.H., Park S., Mendez-Lago M., Rossi F., Villasante A., RA Dimitri P., Karpen G.H., Celniker S.E.; RT "Sequence finishing and mapping of Drosophila melanogaster RT heterochromatin."; RL Science 316:1625-1628(2007). RN [9] {ECO:0007829|PDB:5CFF} RP X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) OF 514-589. RX PubMed=26423004; DOI=10.1038/ncomms9381; RA Jia M., Shan Z., Yang Y., Liu C., Li J., Luo Z.G., Zhang M., Cai Y., RA Wen W., Wang W.; RT "The structural basis of Miranda-mediated Staufen localization during RT Drosophila neuroblast asymmetric division."; RL Nat. Commun. 6:8381-8381(2015). CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AE014297; AAF55723.2; -; Genomic_DNA. DR RefSeq; NP_477291.1; NM_057943.4. DR PDB; 5CFF; X-ray; 2.50 A; A/B/C/D=514-589. DR PDBsum; 5CFF; -. DR AlphaFoldDB; Q9VDR7; -. DR SMR; Q9VDR7; -. DR IntAct; Q9VDR7; 1. DR STRING; 7227.FBpp0083256; -. DR PaxDb; 7227-FBpp0083256; -. DR DNASU; 42379; -. DR EnsemblMetazoa; FBtr0083847; FBpp0083256; FBgn0021776. DR GeneID; 42379; -. DR UCSC; CG12249-RA; d. melanogaster. DR AGR; FB:FBgn0021776; -. DR CTD; 100736249; -. DR FlyBase; FBgn0021776; mira. DR VEuPathDB; VectorBase:FBgn0021776; -. DR eggNOG; ENOG502TAPM; Eukaryota. DR InParanoid; Q9VDR7; -. DR OMA; RYLEMYE; -. DR OrthoDB; 3574512at2759; -. DR PhylomeDB; Q9VDR7; -. DR BioGRID-ORCS; 42379; 0 hits in 1 CRISPR screen. DR GenomeRNAi; 42379; -. DR Proteomes; UP000000803; Chromosome 3R. DR Bgee; FBgn0021776; Expressed in embryonic brain and 61 other cell types or tissues. DR ExpressionAtlas; Q9VDR7; baseline and differential. DR GO; GO:0045179; C:apical cortex; IDA:FlyBase. DR GO; GO:0045177; C:apical part of cell; IDA:FlyBase. DR GO; GO:0016324; C:apical plasma membrane; IDA:FlyBase. DR GO; GO:0045180; C:basal cortex; IDA:FlyBase. DR GO; GO:0045178; C:basal part of cell; IDA:FlyBase. DR GO; GO:0009925; C:basal plasma membrane; IDA:FlyBase. DR GO; GO:0005938; C:cell cortex; IDA:FlyBase. DR GO; GO:0005813; C:centrosome; IDA:FlyBase. DR GO; GO:0005737; C:cytoplasm; IDA:FlyBase. DR GO; GO:0016020; C:membrane; IDA:UniProtKB. DR GO; GO:0017022; F:myosin binding; IPI:FlyBase. DR GO; GO:0008595; P:anterior/posterior axis specification, embryo; IMP:FlyBase. DR GO; GO:0055059; P:asymmetric neuroblast division; TAS:FlyBase. DR GO; GO:0045167; P:asymmetric protein localization involved in cell fate determination; IMP:FlyBase. DR GO; GO:0007402; P:ganglion mother cell fate determination; TAS:FlyBase. DR GO; GO:0007400; P:neuroblast fate determination; TAS:FlyBase. DR GO; GO:0007405; P:neuroblast proliferation; IMP:FlyBase. DR GO; GO:0048477; P:oogenesis; IGI:FlyBase. DR GO; GO:0072697; P:protein localization to cell cortex; IGI:FlyBase. DR GO; GO:1904580; P:regulation of intracellular mRNA localization; IMP:FlyBase. DR GO; GO:0007317; P:regulation of pole plasm oskar mRNA localization; IMP:FlyBase. DR GO; GO:0030162; P:regulation of proteolysis; IMP:FlyBase. DR GO; GO:0007419; P:ventral cord development; HMP:FlyBase. PE 1: Evidence at protein level; KW 3D-structure {ECO:0007829|PDB:5CFF}; Coiled coil {ECO:0000256|SAM:Coils}; KW Reference proteome {ECO:0000313|Proteomes:UP000000803}. FT REGION 22..55 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 88..145 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 763..829 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COILED 162..362 FT /evidence="ECO:0000256|SAM:Coils" FT COILED 491..588 FT /evidence="ECO:0000256|SAM:Coils" FT COILED 665..699 FT /evidence="ECO:0000256|SAM:Coils" FT COMPBIAS 22..45 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 101..132 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 774..791 FT /note="Pro residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" SQ SEQUENCE 829 AA; 93101 MW; BE3AEFD4A454EEAF CRC64; MSFSKAKLKR FNDVDVAICG SPAASNSSAG SAGSATPTAS SAAAAPPTVQ PERKEQIEKF FKDAVRFASS SKEAKEFAIP KEDKKSKGLR LFRTPSLPQR LRFRPTPSHT DTATGSGSGA STAASTPLHS AATTPVKEAK SASRLKGKEA LQYEIRHKNE LIESQLSQLD VLRRHVDQLK EAEAKLREEH ELATSKTDRL IEALTSENLS HKALNEQMGQ EHADLLERLA AMEQQLQQQH DEHERQVEAL VAESEALRLA NELLQTANED RQKVEEQLQA QLSALQADVA QAREHCSLEQ AKTAENIELV ENLQKTNASL LADVVQLKQQ IEQDALSYGQ EAKSCQAELE CLKVERNTLK NDLANKCTLI RSLQDELLDK NCEIDAHCDT IRQLCREQAR HTEQQQAVAK VQQQVESDLE SAVEREKSYW RAELDKRQKL AENELIKIEL EKQDVMVLLE TTNDMLRMRD EKLQKCEEQL RNGIDYYIQL SDALQQQLVQ LKQDMAKTIT EKYNYQLTLT NTRATVNILM ERLKKSDADV EQYRAELESV QLAKGALEQS YLVLQADAEQ LRQQLTESQD ALNALRSSSQ TLQSEVSLKE SLLHELLAGE AETLAKFNQI ANSFQERIDG DAQLAHYHEL RRKDETREAY MVDMKKALDE FATVLQFAQL ELDNKEQMLV KVREECEQLK LENIALKSKQ PGSASLLGTP GKANRSNTTD LEKIEDLLCD SELRSDCEKI TTWLLNSSDK CVRQDTTSEI NELLSAGKSS PRPAPRTPKA PHTPRSPRTP HTPRTPRSAA STPKKTVLFA GKENVPSPPQ KQVLKARNI //