ID   TSKS_HUMAN              Reviewed;         592 AA.
AC   Q9UJT2; Q8WXJ0;
DT   01-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 3.
DT   02-JUN-2021, entry version 135.
DE   RecName: Full=Testis-specific serine kinase substrate;
DE            Short=Testis-specific kinase substrate;
DE   AltName: Full=STK22 substrate 1;
GN   Name=TSKS; Synonyms=STK22S1, TSKS1;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TISSUE SPECIFICITY.
RX   PubMed=11444856; DOI=10.1006/bbrc.2001.5165;
RA   Scorilas A., Yousef G.M., Jung K., Rajpert-De Meyts E., Carsten S.,
RA   Diamandis E.P.;
RT   "Identification and characterization of a novel human testis-specific
RT   kinase substrate gene which is downregulated in testicular tumors.";
RL   Biochem. Biophys. Res. Commun. 285:400-408(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA], PHOSPHORYLATION, INTERACTION WITH TSSK2, TISSUE
RP   SPECIFICITY, AND VARIANT GLY-382.
RX   PubMed=15044604; DOI=10.1093/molehr/gah052;
RA   Hao Z., Jha K.N., Kim Y.H., Vemuganti S., Westbrook V.A., Chertihin O.,
RA   Markgraf K., Flickinger C.J., Coppola M., Herr J.C., Visconti P.E.;
RT   "Expression analysis of the human testis-specific serine/threonine kinase
RT   (TSSK) homologues. A TSSK member is present in the equatorial segment of
RT   human sperm.";
RL   Mol. Hum. Reprod. 10:433-444(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   SUBCELLULAR LOCATION.
RX   PubMed=18495105; DOI=10.1016/j.ydbio.2008.03.043;
RA   Xu B., Hao Z., Jha K.N., Zhang Z., Urekar C., Digilio L., Pulido S.,
RA   Strauss J.F. III, Flickinger C.J., Herr J.C.;
RT   "TSKS concentrates in spermatid centrioles during flagellogenesis.";
RL   Dev. Biol. 319:201-210(2008).
RN   [5]
RP   VARIANT [LARGE SCALE ANALYSIS] ARG-19.
RX   PubMed=16959974; DOI=10.1126/science.1133427;
RA   Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D.,
RA   Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P.,
RA   Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V.,
RA   Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H.,
RA   Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W.,
RA   Velculescu V.E.;
RT   "The consensus coding sequences of human breast and colorectal cancers.";
RL   Science 314:268-274(2006).
CC   -!- FUNCTION: May play a role in testicular physiology, most probably in
CC       the process of spermatogenesis or spermatid development.
CC   -!- INTERACTION:
CC       Q9UJT2; Q7Z3Y8: KRT27; NbExp=3; IntAct=EBI-852101, EBI-3044087;
CC       Q9UJT2; P06753: TPM3; NbExp=3; IntAct=EBI-852101, EBI-355607;
CC       Q9UJT2; Q9BXA7: TSSK1B; NbExp=3; IntAct=EBI-852101, EBI-6423734;
CC       Q9UJT2; Q96PF2: TSSK2; NbExp=9; IntAct=EBI-852101, EBI-852089;
CC       Q9UJT2-2; Q96PF2: TSSK2; NbExp=2; IntAct=EBI-852113, EBI-852089;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC       center, centrosome, centriole {ECO:0000269|PubMed:18495105}.
CC       Note=Concentrates in spermatid centrioles during flagellogenesis.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q9UJT2-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q9UJT2-2; Sequence=VSP_009591, VSP_009592, VSP_009593;
CC   -!- TISSUE SPECIFICITY: Highly expressed in testis. Expressed at low levels
CC       in prostate, female breast, placenta, ovary and thymus.
CC       {ECO:0000269|PubMed:11444856, ECO:0000269|PubMed:15044604}.
CC   -!- PTM: Phosphorylated on serine residue(s) by STK22A/TSSK1 and
CC       STK22B/TSSK2. {ECO:0000269|PubMed:15044604}.
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DR   EMBL; AF200923; AAF12819.3; -; Genomic_DNA.
DR   EMBL; AF411384; AAL60464.1; -; mRNA.
DR   EMBL; BC058862; AAH58862.1; -; mRNA.
DR   CCDS; CCDS12780.1; -. [Q9UJT2-1]
DR   PIR; JC7709; JC7709.
DR   RefSeq; NP_068379.1; NM_021733.1. [Q9UJT2-1]
DR   SMR; Q9UJT2; -.
DR   BioGRID; 121902; 34.
DR   IntAct; Q9UJT2; 32.
DR   STRING; 9606.ENSP00000246801; -.
DR   iPTMnet; Q9UJT2; -.
DR   PhosphoSitePlus; Q9UJT2; -.
DR   BioMuta; TSKS; -.
DR   DMDM; 44888550; -.
DR   EPD; Q9UJT2; -.
DR   MassIVE; Q9UJT2; -.
DR   PaxDb; Q9UJT2; -.
DR   PeptideAtlas; Q9UJT2; -.
DR   PRIDE; Q9UJT2; -.
DR   ProteomicsDB; 84647; -. [Q9UJT2-1]
DR   ProteomicsDB; 84648; -. [Q9UJT2-2]
DR   Antibodypedia; 18691; 121 antibodies.
DR   DNASU; 60385; -.
DR   Ensembl; ENST00000246801; ENSP00000246801; ENSG00000126467. [Q9UJT2-1]
DR   GeneID; 60385; -.
DR   KEGG; hsa:60385; -.
DR   UCSC; uc002ppm.4; human. [Q9UJT2-1]
DR   CTD; 60385; -.
DR   DisGeNET; 60385; -.
DR   GeneCards; TSKS; -.
DR   HGNC; HGNC:30719; TSKS.
DR   HPA; ENSG00000126467; Tissue enriched (testis).
DR   MIM; 608253; gene.
DR   neXtProt; NX_Q9UJT2; -.
DR   OpenTargets; ENSG00000126467; -.
DR   PharmGKB; PA164727339; -.
DR   VEuPathDB; HostDB:ENSG00000126467.10; -.
DR   eggNOG; ENOG502RT0F; Eukaryota.
DR   GeneTree; ENSGT00390000002611; -.
DR   HOGENOM; CLU_033722_0_0_1; -.
DR   InParanoid; Q9UJT2; -.
DR   OMA; PWAPDDP; -.
DR   OrthoDB; 732555at2759; -.
DR   PhylomeDB; Q9UJT2; -.
DR   TreeFam; TF337594; -.
DR   PathwayCommons; Q9UJT2; -.
DR   BioGRID-ORCS; 60385; 7 hits in 1002 CRISPR screens.
DR   GenomeRNAi; 60385; -.
DR   Pharos; Q9UJT2; Tbio.
DR   PRO; PR:Q9UJT2; -.
DR   Proteomes; UP000005640; Chromosome 19.
DR   RNAct; Q9UJT2; protein.
DR   Bgee; ENSG00000126467; Expressed in quadriceps femoris and 112 other tissues.
DR   ExpressionAtlas; Q9UJT2; baseline and differential.
DR   Genevisible; Q9UJT2; HS.
DR   GO; GO:0005814; C:centriole; IDA:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0019901; F:protein kinase binding; IPI:UniProtKB.
DR   GO; GO:0010923; P:negative regulation of phosphatase activity; IDA:UniProtKB.
DR   InterPro; IPR028214; TSKS.
DR   PANTHER; PTHR14351; PTHR14351; 1.
DR   Pfam; PF15358; TSKS; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Cytoplasm; Cytoskeleton; Phosphoprotein;
KW   Reference proteome.
FT   CHAIN           1..592
FT                   /note="Testis-specific serine kinase substrate"
FT                   /id="PRO_0000065665"
FT   REGION          232..308
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          566..592
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        235..264
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        566..585
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         217
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P60531"
FT   MOD_RES         288
FT                   /note="Phosphoserine; by TSSK1 and TSSK2"
FT                   /evidence="ECO:0000250|UniProtKB:O54887"
FT   MOD_RES         316
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P60531"
FT   VAR_SEQ         1..200
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_009591"
FT   VAR_SEQ         201..221
FT                   /note="RSVEDAEIKTNVLKQNSALLE -> MGDSCPAVTFRSQKENRAGLQ (in
FT                   isoform 2)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_009592"
FT   VAR_SEQ         254..259
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_009593"
FT   VARIANT         19
FT                   /note="G -> R (in a colorectal cancer sample; somatic
FT                   mutation; dbSNP:rs149529240)"
FT                   /evidence="ECO:0000269|PubMed:16959974"
FT                   /id="VAR_036272"
FT   VARIANT         167
FT                   /note="E -> K (in dbSNP:rs34701020)"
FT                   /id="VAR_051459"
FT   VARIANT         382
FT                   /note="D -> G (in dbSNP:rs2304202)"
FT                   /evidence="ECO:0000269|PubMed:15044604"
FT                   /id="VAR_051460"
FT   CONFLICT        272
FT                   /note="S -> G (in Ref. 2; AAL60464)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   592 AA;  65050 MW;  87B262601EECE121 CRC64;
     MASVVVKTIW QSKEIHEAGD TPTGVESCSQ LVPEAPRRVT SRAKGIPKKK KAVSFHGVEP
     QMSHQPMHWC LNLKRSSACT NVSLLNLAAM EPTDSTGTDS TVEDLSGQLT LAGPPASPTL
     PWDPDDADIT EILSGVNSGL VRAKDSITSL KEKTNRVNQH VQSLQSECSV LSENLERRRQ
     EAEELEGYCI QLKENCWKVT RSVEDAEIKT NVLKQNSALL EEKLRYLQQQ LQDETPRRQE
     AELQEPEEKQ EPEEKQEPEE KQKPEAGLSW NSLGPAATSQ GCPGPPGSPD KPSRPHGLVP
     AGWGMGPRAG EGPYVSEQEL QKLFTGIEEL RREVSSLTAR WHQEEGAVQE ALRLLGGLGG
     RVDGFLGQWE RAQREQAQTA RDLQELRGRA DELCTMVERS AVSVASLRSE LEGLGPLKPI
     LEEFGRQFQN SRRGPDLSMN LDRSHQGNCA RCASQGSQLS TESLQQLLDR ALTSLVDEVK
     QRGLTPACPS CQRLHKKILE LERQALAKHV RAEALSSTLR LAQDEALRAK NLLLTDKMKP
     EEKMATLDHL HLKMCSLHDH LSNLPLEGST GTMGGGSSAG TPPKQGGSAP EQ
//