ID TRM6_HUMAN Reviewed; 497 AA. AC Q9UJA5; Q76P92; Q9BQV5; Q9ULR7; Q9Y2Z8; DT 02-MAY-2006, integrated into UniProtKB/Swiss-Prot. DT 01-MAY-2000, sequence version 1. DT 25-NOV-2008, entry version 48. DE RecName: Full=tRNA (adenine-N(1)-)-methyltransferase non-catalytic subunit TRM6; DE AltName: Full=tRNA(m1A58)-methyltransferase subunit TRM6; DE Short=tRNA(m1A58)MTase subunit TRM6; GN Name=TRMT6; Synonyms=KIAA1153, TRM6; ORFNames=CGI-09; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; OC Catarrhini; Hominidae; Homo. OX NCBI_TaxID=9606; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND TISSUE RP SPECIFICITY. RC TISSUE=Brain; RX MEDLINE=20039618; PubMed=10574461; DOI=10.1093/dnares/6.5.329; RA Hirosawa M., Nagase T., Ishikawa K., Kikuno R., Nomura N., Ohara O.; RT "Characterization of cDNA clones selected by the GeneMark analysis RT from size-fractionated cDNA libraries from human brain."; RL DNA Res. 6:329-336(1999). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). RX MEDLINE=20272150; PubMed=10810093; DOI=10.1101/gr.10.5.703; RA Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C.; RT "Identification of novel human genes evolutionarily conserved in RT Caenorhabditis elegans by comparative proteomics."; RL Genome Res. 10:703-713(2000). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). RX PubMed=14702039; DOI=10.1038/ng1285; RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S., RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.; RT "Complete sequencing and characterization of 21,243 full-length human RT cDNAs."; RL Nat. Genet. 36:40-45(2004). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RX MEDLINE=21638749; PubMed=11780052; DOI=10.1038/414865a; RA Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., RA Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., RA Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., RA Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M., Brown A.J., RA Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., RA Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., RA Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., RA Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., RA Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., RA Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., RA Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., RA Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., RA Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., RA Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., RA Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., RA Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., RA Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., RA Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., RA Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., RA Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., RA Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., RA Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., RA Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., RA Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., RA Rogers J.; RT "The DNA sequence and comparative analysis of human chromosome 20."; RL Nature 414:865-871(2001). RN [5] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). RC TISSUE=Cervix; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA RT project: the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [6] RP FUNCTION, AND SUBUNIT. RX PubMed=16043508; DOI=10.1261/rna.5040605; RA Ozanick S., Krecic A., Andersland J., Anderson J.T.; RT "The bipartite structure of the tRNA m1A58 methyltransferase from S. RT cerevisiae is conserved in humans."; RL RNA 11:1281-1290(2005). RN [7] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-107, AND MASS RP SPECTROMETRY. RX PubMed=17525332; DOI=10.1126/science.1140321; RA Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, RA Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., RA Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.; RT "ATM and ATR substrate analysis reveals extensive protein networks RT responsive to DNA damage."; RL Science 316:1160-1166(2007). CC -!- FUNCTION: Substrate-binding subunit of tRNA (adenine-N(1)-)- CC methyltransferase, which catalyzes the formation of N(1)- CC methyladenine at position 58 (m1A58) in initiator methionyl-tRNA. CC -!- SUBUNIT: tRNA (adenine-N(1)-)-methyltransferase is a heterodimer CC of TRM6 and TRM61. CC -!- INTERACTION: CC Q96FX7:TRM61; NbExp=1; IntAct=EBI-934061, EBI-934042; CC -!- SUBCELLULAR LOCATION: Nucleus (By similarity). CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=3; CC Name=1; CC IsoId=Q9UJA5-1; Sequence=Displayed; CC Name=2; CC IsoId=Q9UJA5-2; Sequence=VSP_018025; CC Name=3; CC IsoId=Q9UJA5-3; Sequence=VSP_031100, VSP_031101; CC Note=No experimental confirmation available; CC -!- TISSUE SPECIFICITY: Expressed in brain, liver, testis and ovary. CC -!- PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. CC -!- SIMILARITY: Belongs to the TRM6/GCD10 family. CC -!- SEQUENCE CAUTION: CC Sequence=BAA86467.1; Type=Frameshift; Positions=319; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AB032979; BAA86467.1; ALT_INIT; mRNA. DR EMBL; AF132943; AAD27718.1; ALT_FRAME; mRNA. DR EMBL; AK000613; -; NOT_ANNOTATED_CDS; mRNA. DR EMBL; AL035461; CAB55274.1; -; Genomic_DNA. DR EMBL; BC001262; AAH01262.1; -; mRNA. DR RefSeq; NP_057023.2; -. DR UniGene; Hs.128791; -. DR IntAct; Q9UJA5; -. DR PhosphoSite; Q9UJA5; -. DR PeptideAtlas; Q9UJA5; -. DR Ensembl; ENSG00000089195; Homo sapiens. DR GeneID; 51605; -. DR KEGG; hsa:51605; -. DR NMPDR; fig|9606.3.peg.19843; -. DR H-InvDB; HIX0015626; -. DR HGNC; HGNC:20900; TRMT6. DR HOGENOM; Q9UJA5; -. DR HOVERGEN; Q9UJA5; -. DR NextBio; 55494; -. DR ArrayExpress; Q9UJA5; -. DR CleanEx; HS_TRMT6; -. DR GermOnline; ENSG00000089195; Homo sapiens. DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-KW. DR GO; GO:0005515; F:protein binding; IPI:IntAct. DR GO; GO:0003743; F:translation initiation factor activity; IEA:InterPro. DR GO; GO:0006446; P:regulation of translational initiation; IEA:InterPro. DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW. DR InterPro; IPR007316; EIF3_gamma. DR InterPro; IPR017423; tRNA_m1A_mtfrase. DR PANTHER; PTHR12945; EIF3_gamma; 1. DR Pfam; PF04189; Gcd10p; 1. DR PIRSF; PIRSF038170; tRNA_m1A_mtfrase; 1. PE 1: Evidence at protein level; KW Alternative splicing; Nucleus; Phosphoprotein; tRNA processing. FT CHAIN 1 497 tRNA (adenine-N(1)-)-methyltransferase FT non-catalytic subunit TRM6. FT /FTId=PRO_0000233098. FT MOD_RES 107 107 Phosphothreonine. FT VAR_SEQ 344 365 QEKQRRQEEQRKRHLEAAALLS -> SGKNRGRQGRSSGKD FT FWGCRFA (in isoform 3). FT /FTId=VSP_031100. FT VAR_SEQ 366 497 Missing (in isoform 3). FT /FTId=VSP_031101. FT VAR_SEQ 372 497 LIVASRFHPTPLLLSLLDFVAPSRPFVVYCQYKEPLLECYT FT KLRERGGVINLRLSETWLRNYQVLPDRSHPKLLMSGGGGYL FT LSGFTVAMDNLKADTSLKSNASTLESHETEEPAAKKRKCPE FT SDS -> CVDGRRQETPQCMWALLQTDWHSLSPCL (in FT isoform 2). FT /FTId=VSP_018025. FT CONFLICT 299 299 E -> G (in Ref. 2; AAD27718 and 3). SQ SEQUENCE 497 AA; 55799 MW; 920800D0722A6CBB CRC64; MEGSGEQPGP QPQHPGDHRI RDGDFVVLKR EDVFKAVQVQ RRKKVTFEKQ WFYLDNVIGH SYGTAFEVTS GGSLQPKKKR EEPTAETKEA GTDNRNIVDD GKSQKLTQDD IKALKDKGIK GEEIVQQLIE NSTTFRDKTE FAQDKYIKKK KKKYEAIITV VKPSTRILSI MYYAREPGKI NHMRYDTLAQ MLTLGNIRAG NKMIVMETCA GLVLGAMMER MGGFGSIIQL YPGGGPVRAA TACFGFPKSF LSGLYEFPLN KVDSLLHGTF SAKMLSSEPK DSALVEESNG TLEEKQASEQ ENEDSMAEAP ESNHPEDQET METISQDPEH KGPKERGSKK DYIQEKQRRQ EEQRKRHLEA AALLSERNAD GLIVASRFHP TPLLLSLLDF VAPSRPFVVY CQYKEPLLEC YTKLRERGGV INLRLSETWL RNYQVLPDRS HPKLLMSGGG GYLLSGFTVA MDNLKADTSL KSNASTLESH ETEEPAAKKR KCPESDS //