ID DDX20_HUMAN STANDARD; PRT; 824 AA. AC Q9UHI6; Q96F72; Q9NVM3; Q9UF59; Q9UIY0; Q9Y659; DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot. DT 01-DEC-2000, sequence version 2. DT 05-SEP-2006, entry version 66. DE Probable ATP-dependent RNA helicase DDX20 (EC 3.6.1.-) (DEAD box DE protein 20) (DEAD box protein DP 103) (Component of gems 3) (Gemin-3). GN Name=DDX20; Synonyms=DP103, GEMIN3; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; OC Catarrhini; Hominidae; Homo. OX NCBI_TaxID=9606; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION IN THE CORE SMN COMPLEX, RP INTERACTION WITH SNRPB; SNRPD2 AND SNRPD3, AND MASS SPECTROMETRY. RX MEDLINE=20069784; PubMed=10601333; DOI=10.1083/jcb.147.6.1181; RA Charroux B., Pellizzoni L., Perkinson R.A., Shevchenko A., Mann M., RA Dreyfuss G.; RT "Gemin3: a novel DEAD box protein that interacts with SMN, the spinal RT muscular atrophy gene product, and a component of gems."; RL J. Cell Biol. 147:1181-1194(1999). RN [2] RP NUCLEOTIDE SEQUENCE [MRNA], AND INTERACTION WITH EBV EBNA2 AND EBV RP EBNA3C. RX MEDLINE=99315855; PubMed=10383418; DOI=10.1074/jbc.274.27.19136; RA Grundhoff A.T., Kremmer E., Tuereci O., Glieden A., Gindorf C., RA Atz J., Mueller-Lantzsch N., Schubach W.H., Grasser F.A.; RT "Characterization of DP103, a novel DEAD box protein that binds to the RT Epstein-Barr virus nuclear proteins EBNA2 and EBNA3C."; RL J. Biol. Chem. 274:19136-19144(1999). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RX PubMed=14702039; DOI=10.1038/ng1285; RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S., RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.; RT "Complete sequencing and characterization of 21,243 full-length human RT cDNAs."; RL Nat. Genet. 36:40-45(2004). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RX PubMed=16710414; DOI=10.1038/nature04727; RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., RA Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., RA Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., RA McDonald L., Evans R., Phillips K., Atkinson A., Cooper R., Jones C., RA Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., RA Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., RA Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., RA Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., RA Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., RA Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., RA Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., RA Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., RA Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., RA Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., RA Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., RA Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., RA Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., RA Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., RA Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., RA Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., RA Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., RA Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., RA Vaudin M., Sulston J.E., Durbin R., Hubbard T., Wooster R., Dunham I., RA Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., RA Rogers J., Bentley D.R.; RT "The DNA sequence and biological annotation of human chromosome 1."; RL Nature 441:315-321(2006). RN [5] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC TISSUE=Placenta; RX MEDLINE=22388257; PubMed=12477932; DOI=10.1073/pnas.242603899; RA Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., RA Klausner R.D., Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., RA Altschul S.F., Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., RA Hopkins R.F., Jordan H., Moore T., Max S.I., Wang J., Hsieh F., RA Diatchenko L., Marusina K., Farmer A.A., Rubin G.M., Hong L., RA Stapleton M., Soares M.B., Bonaldo M.F., Casavant T.L., Scheetz T.E., RA Brownstein M.J., Usdin T.B., Toshiyuki S., Carninci P., Prange C., RA Raha S.S., Loquellano N.A., Peters G.J., Abramson R.D., Mullahy S.J., RA Bosak S.A., McEwan P.J., McKernan K.J., Malek J.A., Gunaratne P.H., RA Richards S., Worley K.C., Hale S., Garcia A.M., Gay L.J., Hulyk S.W., RA Villalon D.K., Muzny D.M., Sodergren E.J., Lu X., Gibbs R.A., RA Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., RA Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G., RA Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C., RA Rodriguez A.C., Grimwood J., Schmutz J., Myers R.M., RA Butterfield Y.S.N., Krzywinski M.I., Skalska U., Smailus D.E., RA Schnerch A., Schein J.E., Jones S.J.M., Marra M.A.; RT "Generation and initial analysis of more than 15,000 full-length human RT and mouse cDNA sequences."; RL Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002). RN [6] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 189-275. RC TISSUE=Testis; RG The German cDNA consortium; RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases. RN [7] RP INTERACTION WITH RNUT1; SMN1 AND SNRPB. RX MEDLINE=22090571; PubMed=12095920; DOI=10.1093/hmg/11.15.1785; RA Narayanan U., Ospina J.K., Frey M.R., Hebert M.D., Matera A.G.; RT "SMN, the spinal muscular atrophy protein, forms a pre-import snRNP RT complex with snurportin1 and importin beta."; RL Hum. Mol. Genet. 11:1785-1795(2002). RN [8] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-705 AND SER-714, AND RP MASS SPECTROMETRY. RX PubMed=15302935; DOI=10.1073/pnas.0404720101; RA Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., RA Li J., Cohn M.A., Cantley L.C., Gygi S.P.; RT "Large-scale characterization of HeLa cell nuclear phosphoproteins."; RL Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004). CC -!- FUNCTION: The SMN complex plays an essential role in spliceosomal CC snRNP assembly in the cytoplasm and is required for pre-mRNA CC splicing in the nucleus. It may also play a role in the metabolism CC of snoRNPs. CC -!- SUBUNIT: Part of the core SMN complex that contains SMN1, SIP1, CC GEMIN2, GEMIN3, GEMIN4, GEMIN5, GEMIN6 and GEMIN7. Interacts CC directly with SMN1 and with several spliceosomal snRNP core Sm CC proteins, including RNUT1, SNRPB, SNRPD2 and SNRPD3. Interacts CC with EBV EBNA2 and EBNA3C. CC -!- INTERACTION: CC P48634:BAT2; NbExp=1; IntAct=EBI-347658, EBI-347545; CC -!- SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Localized in subnuclear CC structures next to coiled bodies, called Gemini of Cajal bodies CC (Gems). CC -!- TISSUE SPECIFICITY: Ubiquitous. CC -!- SIMILARITY: Belongs to the DEAD box helicase family. CC -!- SIMILARITY: Contains 1 helicase ATP-binding domain. CC -!- SIMILARITY: Contains 1 helicase C-terminal domain. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AF171063; AAF14544.1; -; mRNA. DR EMBL; AF106019; AAD42744.1; -; mRNA. DR EMBL; AK001506; BAA91727.1; -; mRNA. DR EMBL; AL049557; CAB55686.2; -; Genomic_DNA. DR EMBL; BC011556; AAH11556.1; -; mRNA. DR EMBL; AL133598; CAB63734.2; -; mRNA. DR PIR; T43476; T43476. DR HSSP; P10081; 1QDE. DR IntAct; Q9UHI6; -. DR Ensembl; ENSG00000064703; Homo sapiens. DR H-InvDB; HIX0000887; -. DR HGNC; HGNC:2743; DDX20. DR MIM; 606168; gene. DR ArrayExpress; Q9UHI6; -. DR RZPD-ProtExp; IOH22645; -. DR RZPD-ProtExp; IOH35421; -. DR RZPD-ProtExp; T1237; -. DR GO; GO:0005737; C:cytoplasm; TAS. DR GO; GO:0005856; C:cytoskeleton; TAS. DR GO; GO:0005634; C:nucleus; TAS. DR GO; GO:0004004; F:ATP-dependent RNA helicase activity; TAS. DR GO; GO:0005515; F:protein binding; IPI. DR GO; GO:0000244; P:assembly of spliceosomal tri-snRNP; TAS. DR GO; GO:0043065; P:positive regulation of apoptosis; ISS. DR GO; GO:0006396; P:RNA processing; TAS. DR InterPro; IPR001410; DEAD. DR InterPro; IPR011545; DEAD/DEAH_N. DR InterPro; IPR000629; DEAD_box. DR InterPro; IPR001650; Helicase_C. DR Pfam; PF00270; DEAD; 1. DR Pfam; PF00271; Helicase_C; 1. DR SMART; SM00487; DEXDc; 1. DR SMART; SM00490; HELICc; 1. DR PROSITE; PS00039; DEAD_ATP_HELICASE; 1. DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1. DR PROSITE; PS51194; HELICASE_CTER; 1. DR PROSITE; PS51195; Q_MOTIF; 1. KW ATP-binding; DNA-binding; Helicase; Hydrolase; mRNA processing; KW mRNA splicing; Nuclear protein; Nucleotide-binding; Phosphorylation; KW Spliceosome. FT CHAIN 1 824 Probable ATP-dependent RNA helicase FT DDX20. FT /FTId=PRO_0000055025. FT DOMAIN 93 264 Helicase ATP-binding. FT DOMAIN 299 448 Helicase C-terminal. FT NP_BIND 106 113 ATP (Potential). FT REGION 456 548 SMN interacting. FT MOTIF 62 90 Q motif. FT MOTIF 211 214 DEAD box. FT MOD_RES 705 705 Phosphothreonine. FT MOD_RES 714 714 Phosphoserine. FT CONFLICT 5 5 F -> V (in Ref. 2). FT CONFLICT 279 279 Y -> C (in Ref. 3). FT CONFLICT 636 636 I -> T (in Ref. 5). FT CONFLICT 639 639 R -> K (in Ref. 1). FT CONFLICT 659 659 Y -> H (in Ref. 2). FT CONFLICT 676 676 G -> S (in Ref. 1). FT CONFLICT 703 703 S -> T (in Ref. 1). SQ SEQUENCE 824 AA; 92241 MW; 76712F014B2A0CF2 CRC64; MAAAFEASGA LAAVATAMPA EHVAVQVPAP EPTPGPVRIL RTAQDLSSPR TRTGDVLLAE PADFESLLLS RPVLEGLRAA GFERPSPVQL KAIPLGRCGL DLIVQAKSGT GKTCVFSTIA LDSLVLENLS TQILILAPTR EIAVQIHSVI TAIGIKMEGL ECHVFIGGTP LSQDKTRLKK CHIAVGSPGR IKQLIELDYL NPGSIRLFIL DEADKLLEEG SFQEQINWIY SSLPASKQML AVSATYPEFL ANALTKYMRD PTFVRLNSSD PSLIGLKQYY KVVNSYPLAH KVFEEKTQHL QELFSRIPFN QALVFSNLHS RAQHLADILS SKGFPAECIS GNMNQNQRLD AMAKLKHFHC RVLISTDLTS RGIDAEKVNL VVNLDVPLDW ETYMHRIGRA GRFGTLGLTV TYCCRGEEEN MMMRIAQKCN INLLPLPDPI PSGLMEECVD WDVEVKAAVH TYGIASVPNQ PLKKQIQKIE RTLQIQKAHG DHMASSRNNS VSGLSVKSKN NTKQKLPVKS HSECGIIEKA TSPKELGCDR QSEEQMKNSV QTPVENSTNS QHQVKEALPV SLPQIPCLSS FKIHQPYTLT FAELVEDYEH YIKEGLEKPV EIIRHYTGPG DQTVNPQNGF VRNKVIEQRV PVLASSSQSG DSESDSDSYS SRTSSQSKGN KSYLEGSSDN QLKDSESTPV DDRISLEQPP NGSDTPNPEK YQESPGIQMK TRLKEGASQR AKQSRRNLPR RSSFRLQTEA QEDDWYDCHR EIRLSFSDTY QDYEEYWRAY YRAWQEYYAA ASHSYYWNAQ RHPSWMAAYH MNTIYLQEMM HSNQ //