ID DD20_HUMAN STANDARD; PRT; 824 AA. AC Q9UHI6; Q96F72; Q9NVM3; Q9UF59; Q9UIY0; Q9Y659; DT 16-OCT-2001 (Rel. 40, Created) DT 16-OCT-2001 (Rel. 40, Last sequence update) DT 28-FEB-2003 (Rel. 41, Last annotation update) DE Probable ATP-dependent RNA helicase DDX20 (DEAD-box protein 20) (DEAD- DE box protein DP 103) (Component of gems 3) (Gemin3). GN DDX20 OR GEMIN3 OR DP103. OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. OX NCBI_TaxID=9606; RN [1] RP SEQUENCE FROM N.A., AND MASS SPECTROMETRY. RX MEDLINE=20069784; PubMed=10601333; RA Charroux B., Pellizzoni L., Perkinson R.A., Shevchenko A., Mann M., RA Dreyfuss G.; RT "Gemin3: a novel DEAD box protein that interacts with SMN, the spinal RT muscular atrophy gene product, and a component of gems."; RL J. Cell Biol. 147:1181-1194(1999). RN [2] RP SEQUENCE FROM N.A. RX MEDLINE=99315855; PubMed=10383418; RA Grundhoff A.T., Kremmer E., Tureci O., Glieden A., Gindorf C., Atz J., RA Mueller-Lantzsch N., Schubach W.H., Grasser F.A.; RT "Characterization of DP103, a novel DEAD box protein that binds to the RT Epstein-Barr virus nuclear proteins EBNA2 and EBNA3C."; RL J. Biol. Chem. 274:19136-19144(1999). RN [3] RP SEQUENCE FROM N.A. RA Coville G.; RL Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases. RN [4] RP SEQUENCE FROM N.A. RA Isogai T., Ota T., Hayashi K., Sugiyama T., Otsuki T., Suzuki Y., RA Nishikawa T., Nagai K., Sugano S., Ishibashi T., Fujimori K., RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Ishii S., Kawai Y., RA Saito K., Yamamoto J., Wakamatsu A., Nakamura Y., Nagahari K., RA Masuho Y., Kanehori K.; RT "NEDO human cDNA sequencing project."; RL Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases. RN [5] RP SEQUENCE FROM N.A. RC TISSUE=Placenta; RA Strausberg R.; RL Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases. RN [6] RP SEQUENCE OF 393-824 FROM N.A. RC TISSUE=Testis; RA Ansorge W., Wirkner U., Mewes H.-W., Gassenhuber J., Wiemann S.; RL Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: THE SMN COMPLEX PLAYS AN ESSENTIAL ROLE IN SPLICEOSOMAL CC SNRNP ASSEMBLY IN THE CYTOPLASM, AND IS REQUIRED FOR PRE-MRNA CC SPLICING IN THE NUCLEUS. CC -!- SUBUNIT: FORMS A STABLE HETEROMERIC COMPLEX WITH SURVIVAL OF MOTOR CC NEURON PROTEIN (SMN), GEMIN2 AND GEMIN4. INTERACTS DIRECTLY WITH CC SMN, SM B, SM D2 AND SM D3. INTERACTS WITH EBV EBNA2 AND EBNA3C. CC -!- SUBCELLULAR LOCATION: CYTOPLASMIC AND NUCLEAR. LOCALIZED IN CC SUBNUCLEAR STRUCTURES NEXT TO COILED BODIES, CALLED GEMS, WHICH CC ARE HIGHLY ENRICHED IN SPLICEOSOMAL SNRNPS. CC -!- TISSUE SPECIFICITY: Ubiquitous. CC -!- SIMILARITY: BELONGS TO THE DEAD BOX HELICASE FAMILY. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AF171063; AAF14544.1; -. DR EMBL; AF106019; AAD42744.1; -. DR EMBL; AL049557; CAB55686.2; -. DR EMBL; AK001506; BAA91727.1; -. DR EMBL; BC011556; AAH11556.1; -. DR EMBL; AL133598; CAB63734.1; -. DR HSSP; Q58083; 1HV8. DR Genew; HGNC:2743; DDX20. DR MIM; 606168; -. DR InterPro; IPR001410; DEAD. DR InterPro; IPR000629; DEAD_box. DR InterPro; IPR001650; Helicase_C. DR Pfam; PF00270; DEAD; 1. DR Pfam; PF00271; helicase_C; 1. DR SMART; SM00487; DEXDc; 1. DR SMART; SM00490; HELICc; 1. DR PROSITE; PS00039; DEAD_ATP_HELICASE; 1. KW Helicase; ATP-binding; DNA-binding; Nuclear protein; mRNA processing; KW Spliceosome. FT NP_BIND 106 113 ATP (POTENTIAL). FT SITE 211 214 DEAD BOX. FT DOMAIN 456 548 SMN INTERACTING. FT CONFLICT 5 5 F -> V (IN REF. 2). FT CONFLICT 279 279 Y -> C (IN REF. 4). FT CONFLICT 393 403 YMHRIGRAGRF -> MIYYLTFSLLL (IN REF. 6). FT CONFLICT 636 636 I -> T (IN REF. 5 AND 6). FT CONFLICT 639 639 R -> K (IN REF. 1). FT CONFLICT 659 659 Y -> H (IN REF. 2). FT CONFLICT 676 676 G -> S (IN REF. 1). FT CONFLICT 703 703 S -> T (IN REF. 1). SQ SEQUENCE 824 AA; 92240 MW; 76712F014B2A0CF2 CRC64; MAAAFEASGA LAAVATAMPA EHVAVQVPAP EPTPGPVRIL RTAQDLSSPR TRTGDVLLAE PADFESLLLS RPVLEGLRAA GFERPSPVQL KAIPLGRCGL DLIVQAKSGT GKTCVFSTIA LDSLVLENLS TQILILAPTR EIAVQIHSVI TAIGIKMEGL ECHVFIGGTP LSQDKTRLKK CHIAVGSPGR IKQLIELDYL NPGSIRLFIL DEADKLLEEG SFQEQINWIY SSLPASKQML AVSATYPEFL ANALTKYMRD PTFVRLNSSD PSLIGLKQYY KVVNSYPLAH KVFEEKTQHL QELFSRIPFN QALVFSNLHS RAQHLADILS SKGFPAECIS GNMNQNQRLD AMAKLKHFHC RVLISTDLTS RGIDAEKVNL VVNLDVPLDW ETYMHRIGRA GRFGTLGLTV TYCCRGEEEN MMMRIAQKCN INLLPLPDPI PSGLMEECVD WDVEVKAAVH TYGIASVPNQ PLKKQIQKIE RTLQIQKAHG DHMASSRNNS VSGLSVKSKN NTKQKLPVKS HSECGIIEKA TSPKELGCDR QSEEQMKNSV QTPVENSTNS QHQVKEALPV SLPQIPCLSS FKIHQPYTLT FAELVEDYEH YIKEGLEKPV EIIRHYTGPG DQTVNPQNGF VRNKVIEQRV PVLASSSQSG DSESDSDSYS SRTSSQSKGN KSYLEGSSDN QLKDSESTPV DDRISLEQPP NGSDTPNPEK YQESPGIQMK TRLKEGASQR AKQSRRNLPR RSSFRLQTEA QEDDWYDCHR EIRLSFSDTY QDYEEYWRAY YRAWQEYYAA ASHSYYWNAQ RHPSWMAAYH MNTIYLQEMM HSNQ //