ID   DNJB4_HUMAN             Reviewed;         337 AA.
AC   Q9UDY4; B2R824; Q13431;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   02-NOV-2016, entry version 139.
DE   RecName: Full=DnaJ homolog subfamily B member 4;
DE   AltName: Full=Heat shock 40 kDa protein 1 homolog;
DE            Short=HSP40 homolog;
DE            Short=Heat shock protein 40 homolog;
DE   AltName: Full=Human liver DnaJ-like protein;
GN   Name=DNAJB4; Synonyms=DNAJW, HLJ1;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, AND TISSUE SPECIFICITY.
RC   TISSUE=Liver;
RX   PubMed=9546042; DOI=10.1016/S0167-4838(97)00207-0;
RA   Hoe K.L., Won M., Chung K.S., Jang Y.J., Lee S.B., Kim D.U., Lee J.W.,
RA   Yun J.H., Yoo H.S.;
RT   "Isolation of a new member of DnaJ-like heat shock protein 40 (Hsp40)
RT   from human liver.";
RL   Biochim. Biophys. Acta 1383:4-8(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA   Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA   Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA   Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA   Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA   Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA   Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA   Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA   Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA   Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA   Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA   Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA   Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA   Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA   Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA   Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA   Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA   Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA   Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA   Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA   Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA   Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA   Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA   Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA   Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA   Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA   Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Lymph;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [5]
RP   PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   TISSUE=Liver;
RA   Won M., Moon K.-M., Lee C.-E., Yoo H.-S.;
RL   Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   PROTEIN SEQUENCE OF 2-13; 45-59; 166-177; 219-236; 260-275 AND
RP   293-302, CLEAVAGE OF INITIATOR METHIONINE, AND IDENTIFICATION BY MASS
RP   SPECTROMETRY.
RC   TISSUE=Embryonic kidney;
RA   Bienvenut W.V., Waridel P., Quadroni M.;
RL   Submitted (MAR-2009) to UniProtKB.
RN   [7]
RP   HOMODIMERIZATION.
RX   PubMed=15661747; DOI=10.1074/jbc.M408349200;
RA   Borges J.C., Fischer H., Craievich A.F., Ramos C.H.I.;
RT   "Low resolution structural study of two human HSP40 chaperones in
RT   solution. DJA1 from subfamily A and DJB4 from subfamily B have
RT   different quaternary structures.";
RL   J. Biol. Chem. 280:13671-13681(2005).
RN   [8]
RP   SUBCELLULAR LOCATION, AND INTERACTION WITH OPRM1.
RX   PubMed=16542645; DOI=10.1016/j.brainres.2006.01.125;
RA   Ancevska-Taneva N., Onoprishvili I., Andria M.L., Hiller J.M.,
RA   Simon E.J.;
RT   "A member of the heat shock protein 40 family, hlj1, binds to the
RT   carboxyl tail of the human mu opioid receptor.";
RL   Brain Res. 1081:28-33(2006).
RN   [9]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-122, AND IDENTIFICATION
RP   BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Cervix carcinoma;
RX   PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA   Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA   Elledge S.J., Gygi S.P.;
RT   "A quantitative atlas of mitotic phosphorylation.";
RL   Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN   [10]
RP   SUBCELLULAR LOCATION.
RX   PubMed=18837411;
RA   Lin X., Ma L., Wang J., Tan Y., Wen Q., Luo W., Su J., Lin Y.,
RA   Wang X.;
RT   "Preparation of the anti-HLJ1 monoclonal antibodies and establishment
RT   of method for detection of the antigen.";
RL   Sheng Wu Gong Cheng Xue Bao 24:1293-1299(2008).
RN   [11]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-122 AND SER-148, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Cervix carcinoma;
RX   PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA   Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA   Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S.,
RA   Mann M.;
RT   "Quantitative phosphoproteomics reveals widespread full
RT   phosphorylation site occupancy during mitosis.";
RL   Sci. Signal. 3:RA3-RA3(2010).
RN   [12]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA   Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA   Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT   "Initial characterization of the human central proteome.";
RL   BMC Syst. Biol. 5:17-17(2011).
RN   [13]
RP   INTERACTION WITH SDIM1.
RX   PubMed=21255413; DOI=10.1186/1750-1326-6-9;
RA   Lei J.X., Cassone C.G., Luebbert C., Liu Q.Y.;
RT   "A novel neuron-enriched protein SDIM1 is down regulated in
RT   Alzheimer's brains and attenuates cell death induced by DNAJB4 over-
RT   expression in neuro-progenitor cells.";
RL   Mol. Neurodegener. 6:9-9(2011).
CC   -!- FUNCTION: Probable chaperone.
CC   -!- SUBUNIT: Homodimer. The C-terminal section interacts with the C-
CC       terminal tail of OPRM1. Interacts also with SDIM1.
CC       {ECO:0000269|PubMed:16542645, ECO:0000269|PubMed:21255413}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:18837411}.
CC       Cell membrane {ECO:0000269|PubMed:16542645}. Note=Cytoplasmic
CC       according to PubMed:18837411 and membrane-associated according to
CC       PubMed:16542645.
CC   -!- TISSUE SPECIFICITY: Expressed in heart, pancreas and skeletal
CC       muscle, and to a lesser extent in brain, placenta and liver.
CC       {ECO:0000269|PubMed:9546042}.
CC   -!- INDUCTION: By heat shock. {ECO:0000269|PubMed:9546042}.
CC   -!- SIMILARITY: Contains 1 J domain. {ECO:0000255|PROSITE-
CC       ProRule:PRU00286}.
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DR   EMBL; U40992; AAC14483.2; -; mRNA.
DR   EMBL; AK313205; BAG36021.1; -; mRNA.
DR   EMBL; CH471059; EAX06354.1; -; Genomic_DNA.
DR   EMBL; BC034721; AAH34721.1; -; mRNA.
DR   EMBL; U41290; AAB07346.1; ALT_FRAME; Genomic_DNA.
DR   CCDS; CCDS684.1; -.
DR   PIR; G02272; G02272.
DR   RefSeq; NP_001304028.1; NM_001317099.1.
DR   RefSeq; NP_001304029.1; NM_001317100.1.
DR   RefSeq; NP_001304030.1; NM_001317101.1.
DR   RefSeq; NP_001304031.1; NM_001317102.1.
DR   RefSeq; NP_001304032.1; NM_001317103.1.
DR   RefSeq; NP_008965.2; NM_007034.4.
DR   UniGene; Hs.13852; -.
DR   UniGene; Hs.690049; -.
DR   ProteinModelPortal; Q9UDY4; -.
DR   SMR; Q9UDY4; -.
DR   BioGrid; 116263; 57.
DR   IntAct; Q9UDY4; 24.
DR   MINT; MINT-1142940; -.
DR   STRING; 9606.ENSP00000359799; -.
DR   iPTMnet; Q9UDY4; -.
DR   PhosphoSitePlus; Q9UDY4; -.
DR   BioMuta; DNAJB4; -.
DR   DMDM; 8928155; -.
DR   EPD; Q9UDY4; -.
DR   MaxQB; Q9UDY4; -.
DR   PaxDb; Q9UDY4; -.
DR   PeptideAtlas; Q9UDY4; -.
DR   PRIDE; Q9UDY4; -.
DR   DNASU; 11080; -.
DR   Ensembl; ENST00000370763; ENSP00000359799; ENSG00000162616.
DR   GeneID; 11080; -.
DR   KEGG; hsa:11080; -.
DR   UCSC; uc001dij.4; human.
DR   CTD; 11080; -.
DR   DisGeNET; 11080; -.
DR   GeneCards; DNAJB4; -.
DR   HGNC; HGNC:14886; DNAJB4.
DR   HPA; CAB004995; -.
DR   HPA; HPA028383; -.
DR   HPA; HPA028385; -.
DR   MIM; 611327; gene.
DR   neXtProt; NX_Q9UDY4; -.
DR   OpenTargets; ENSG00000162616; -.
DR   PharmGKB; PA27416; -.
DR   eggNOG; KOG0714; Eukaryota.
DR   eggNOG; COG0484; LUCA.
DR   GeneTree; ENSGT00760000118947; -.
DR   HOVERGEN; HBG066727; -.
DR   InParanoid; Q9UDY4; -.
DR   KO; K09510; -.
DR   OMA; MDGRTIP; -.
DR   OrthoDB; EOG091G0DLS; -.
DR   PhylomeDB; Q9UDY4; -.
DR   TreeFam; TF105141; -.
DR   BioCyc; ZFISH:ENSG00000162616-MONOMER; -.
DR   GeneWiki; DNAJB4; -.
DR   GenomeRNAi; 11080; -.
DR   PRO; PR:Q9UDY4; -.
DR   Proteomes; UP000005640; Chromosome 1.
DR   Bgee; ENSG00000162616; -.
DR   CleanEx; HS_DNAJB4; -.
DR   ExpressionAtlas; Q9UDY4; baseline and differential.
DR   Genevisible; Q9UDY4; HS.
DR   GO; GO:0005737; C:cytoplasm; IDA:HPA.
DR   GO; GO:0005829; C:cytosol; IDA:UniProtKB.
DR   GO; GO:0070062; C:extracellular exosome; IDA:UniProtKB.
DR   GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR   GO; GO:0005886; C:plasma membrane; IDA:HPA.
DR   GO; GO:0051087; F:chaperone binding; IPI:UniProtKB.
DR   GO; GO:0051082; F:unfolded protein binding; TAS:ProtInc.
DR   GO; GO:0006457; P:protein folding; IEA:InterPro.
DR   GO; GO:0009408; P:response to heat; TAS:ProtInc.
DR   GO; GO:0006986; P:response to unfolded protein; TAS:ProtInc.
DR   CDD; cd06257; DnaJ; 1.
DR   Gene3D; 1.10.287.110; -; 1.
DR   InterPro; IPR002939; DnaJ_C.
DR   InterPro; IPR001623; DnaJ_domain.
DR   InterPro; IPR018253; DnaJ_domain_CS.
DR   InterPro; IPR008971; HSP40/DnaJ_pept-bd.
DR   Pfam; PF00226; DnaJ; 1.
DR   Pfam; PF01556; DnaJ_C; 1.
DR   PRINTS; PR00625; JDOMAIN.
DR   SMART; SM00271; DnaJ; 1.
DR   SUPFAM; SSF46565; SSF46565; 1.
DR   SUPFAM; SSF49493; SSF49493; 2.
DR   PROSITE; PS00636; DNAJ_1; 1.
DR   PROSITE; PS50076; DNAJ_2; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Chaperone; Complete proteome; Cytoplasm;
KW   Direct protein sequencing; Membrane; Phosphoprotein;
KW   Reference proteome; Stress response.
FT   INIT_MET      1      1       Removed. {ECO:0000269|Ref.6}.
FT   CHAIN         2    337       DnaJ homolog subfamily B member 4.
FT                                /FTId=PRO_0000071021.
FT   DOMAIN        2     70       J. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00286}.
FT   MOD_RES     122    122       Phosphoserine.
FT                                {ECO:0000244|PubMed:18669648,
FT                                ECO:0000244|PubMed:20068231}.
FT   MOD_RES     148    148       Phosphoserine.
FT                                {ECO:0000244|PubMed:20068231}.
SQ   SEQUENCE   337 AA;  37807 MW;  C7A9C613F73BCDAC CRC64;
     MGKDYYCILG IEKGASDEDI KKAYRKQALK FHPDKNKSPQ AEEKFKEVAE AYEVLSDPKK
     REIYDQFGEE GLKGGAGGTD GQGGTFRYTF HGDPHATFAA FFGGSNPFEI FFGRRMGGGR
     DSEEMEIDGD PFSAFGFSMN GYPRDRNSVG PSRLKQDPPV IHELRVSLEE IYSGCTKRMK
     ISRKRLNADG RSYRSEDKIL TIEIKKGWKE GTKITFPREG DETPNSIPAD IVFIIKDKDH
     PKFKRDGSNI IYTAKISLRE ALCGCSINVP TLDGRNIPMS VNDIVKPGMR RRIIGYGLPF
     PKNPDQRGDL LIEFEVSFPD TISSSSKEVL RKHLPAS
//