ID   Q9U1L3_DROME            Unreviewed;       368 AA.
AC   Q9U1L3;
DT   01-MAY-2000, integrated into UniProtKB/TrEMBL.
DT   01-MAY-2000, sequence version 1.
DT   02-DEC-2020, entry version 102.
DE   RecName: Full=Translocating chain-associated membrane protein {ECO:0000256|PIRNR:PIRNR005449};
GN   Name=TRAM {ECO:0000313|FlyBase:FBgn0040340};
GN   Synonyms=EG:BACR7A4.5 {ECO:0000313|EMBL:CAB65875.1};
GN   ORFNames=CG11642 {ECO:0000313|FlyBase:FBgn0040340};
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Holometabola; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227 {ECO:0000313|EMBL:CAB65875.1};
RN   [1] {ECO:0000313|EMBL:CAB65875.1}
RP   NUCLEOTIDE SEQUENCE.
RA   Papagiannakis G., Spanos L., Bolshakov V., Siden-Kiamos I., Louis C.;
RT   "Sequencing the distal X chromosome of Drosophila melanogaster.";
RL   Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:CAB65875.1}
RP   NUCLEOTIDE SEQUENCE.
RA   Benos P.;
RL   Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC       pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC   -!- SIMILARITY: Belongs to the TRAM family. {ECO:0000256|ARBA:ARBA00005999,
CC       ECO:0000256|PIRNR:PIRNR005449}.
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DR   EMBL; AL109630; CAB65875.1; -; Genomic_DNA.
DR   IntAct; Q9U1L3; 1.
DR   PRIDE; Q9U1L3; -.
DR   FlyBase; FBgn0040340; TRAM.
DR   HOGENOM; CLU_062830_0_0_1; -.
DR   Bgee; FBgn0040340; Expressed in spermathecum and 53 other tissues.
DR   ExpressionAtlas; Q9U1L3; differential.
DR   GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR   GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; IBA:GO_Central.
DR   GO; GO:0045048; P:protein insertion into ER membrane; IBA:GO_Central.
DR   GO; GO:0006616; P:SRP-dependent cotranslational protein targeting to membrane, translocation; IEA:InterPro.
DR   InterPro; IPR006634; TLC-dom.
DR   InterPro; IPR013599; TRAM1.
DR   InterPro; IPR016447; Translocation_assoc_membrane.
DR   PANTHER; PTHR12371; PTHR12371; 1.
DR   Pfam; PF08390; TRAM1; 1.
DR   Pfam; PF03798; TRAM_LAG1_CLN8; 1.
DR   PIRSF; PIRSF005449; Translocation_assoc_membrane; 1.
DR   SMART; SM00724; TLC; 1.
DR   PROSITE; PS50922; TLC; 1.
PE   3: Inferred from homology;
KW   Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|PROSITE-
KW   ProRule:PRU00205, ECO:0000256|SAM:Phobius};
KW   Protein transport {ECO:0000256|ARBA:ARBA00022927,
KW   ECO:0000256|PIRNR:PIRNR005449};
KW   Translocation {ECO:0000256|ARBA:ARBA00023010,
KW   ECO:0000256|PIRNR:PIRNR005449};
KW   Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|PROSITE-
KW   ProRule:PRU00205, ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW   ECO:0000256|SAM:Phobius};
KW   Transport {ECO:0000256|ARBA:ARBA00022448, ECO:0000256|PIRNR:PIRNR005449}.
FT   TRANSMEM        31..56
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        89..108
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        129..147
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        204..221
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        227..249
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        261..285
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        291..310
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          123..318
FT                   /note="TLC"
FT                   /evidence="ECO:0000259|PROSITE:PS50922"
FT   REGION          324..368
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        324..354
FT                   /note="Polyampholyte"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   368 AA;  41766 MW;  693795FFFC2ED4A6 CRC64;
     MAIKPGLGRK TSNKNPPILS HEFVIQNHAD IISCVAMVFV VGLMNESTAA FASAFISLHH
     NVSGEDPSRE QPYGKPYTYI AGIKDYCAIF FYTLTCIIMH AIIQEFVLDN ISKKLHLSKF
     KLARFNESGQ LVAFYLLSFV WGAHVLLKEG YLGQVAQLWE GFPDHPMSFL HKFYFVVQLA
     YYLHMLPELY FQKIKTKEEQ QPKIVHSISG FTLIVLAYTL SFQRLALVLL TLHYFSELLS
     HVFQLIGVFD REERLAKLRV VNNAVFFLIR FATSVIGVLT LYYGIGGVRS LLALGGLIAL
     QGYLVFSFIT EQLRAKREAK KEAKREAKLA LQTKKPAKTP KDKVKRKKES DLPEADQTSP
     SPIKQKLK
//