ID MED18_ARATH Reviewed; 219 AA. AC Q9SJZ6; DT 11-JUL-2012, integrated into UniProtKB/Swiss-Prot. DT 01-MAY-2000, sequence version 1. DT 10-MAY-2017, entry version 84. DE RecName: Full=Mediator of RNA polymerase II transcription subunit 18 {ECO:0000303|PubMed:17560376}; GN Name=MED18 {ECO:0000303|PubMed:17560376}; GN Synonyms=MED18_1 {ECO:0000303|PubMed:22021418}; GN OrderedLocusNames=At2g22370 {ECO:0000312|Araport:AT2G22370}; GN ORFNames=F14M13.23 {ECO:0000312|EMBL:AAD22361.1}; OS Arabidopsis thaliana (Mouse-ear cress). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae; OC Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; OC Arabidopsis. OX NCBI_TaxID=3702; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Columbia; RX PubMed=10617197; DOI=10.1038/45471; RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., RA Moffat K.S., Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., RA Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., RA Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., RA Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., RA Venter J.C.; RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis RT thaliana."; RL Nature 402:761-768(1999). RN [2] RP GENOME REANNOTATION. RC STRAIN=cv. Columbia; RG The Arabidopsis Information Portal (Araport); RL Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases. RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RA Bautista V.R., Kim C.J., Chen H., Quinitio C., Ecker J.R.; RT "Arabidopsis ORF clones."; RL Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases. RN [4] RP IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT, AND NOMENCLATURE. RX PubMed=17560376; DOI=10.1016/j.molcel.2007.05.007; RA Baeckstroem S., Elfving N., Nilsson R., Wingsle G., Bjoerklund S.; RT "Purification of a plant mediator from Arabidopsis thaliana identifies RT PFT1 as the Med25 subunit."; RL Mol. Cell 26:717-729(2007). RN [5] RP IDENTIFICATION, AND NOMENCLATURE. RX PubMed=22021418; DOI=10.1104/pp.111.188300; RA Mathur S., Vyas S., Kapoor S., Tyagi A.K.; RT "The Mediator complex in plants: structure, phylogeny, and expression RT profiling of representative genes in a dicot (Arabidopsis) and a RT monocot (rice) during reproduction and abiotic stress."; RL Plant Physiol. 157:1609-1627(2011). RN [6] RP FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH YY1; ABI4 AND SUF4, RP AND SUBCELLULAR LOCATION. RC STRAIN=cv. Columbia; RX PubMed=24451981; DOI=10.1038/ncomms4064; RA Lai Z., Schluttenhofer C.M., Bhide K., Shreve J., Thimmapuram J., RA Lee S.Y., Yun D.-J., Mengiste T.; RT "MED18 interaction with distinct transcription factors regulates RT multiple plant functions."; RL Nat. Commun. 5:3064-3064(2014). CC -!- FUNCTION: Component of the Mediator complex, a coactivator CC involved in the regulated transcription of nearly all RNA CC polymerase II-dependent genes. Mediator functions as a bridge to CC convey information from gene-specific regulatory proteins to the CC basal RNA polymerase II transcription machinery. The Mediator CC complex, having a compact conformation in its free form, is CC recruited to promoters by direct interactions with regulatory CC proteins and serves for the assembly of a functional pre- CC initiation complex with RNA polymerase II and the general CC transcription factors (PubMed:22021418, PubMed:24451981). Involved CC in the regulation of histone H3 lysine tri-methylation (H3K36me3). CC Associates with the promoter, coding and terminator regions of CC target genes suggesting its function in transcription initiation, CC elongation and termination. Multifunctional protein which CC regulates plant immunity, especially during necrotrophic fungal CC infection (e.g. B.cinerea and A.brassicicola), flowering time and CC responses to hormones (e.g. abscisic acid ABA and ethylene) CC through interactions with distinct transcription factors CC (PubMed:24451981). {ECO:0000269|PubMed:22021418, CC ECO:0000269|PubMed:24451981}. CC -!- SUBUNIT: Component of the Mediator complex (PubMed:17560376). CC Interacts with YY1 to suppress disease susceptibility via the CC repression of genes glutaredoxins GRX480, GRXS13 and thioredoxin CC TRX-h5. Binds to ABI4 to regulate abscisic acid responses; CC recruited by ABI4 to ABI5 promoter in the presence of abscisic CC acid (ABA). Interacts with SUF4 to regulate flowering time; CC recruited by SUF4 to FLC promoter (PubMed:24451981). CC {ECO:0000269|PubMed:17560376, ECO:0000269|PubMed:24451981}. CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:24451981}. CC -!- DISRUPTION PHENOTYPE: Deregulated expression of glutaredoxins CC GRX480, GRXS13 and thioredoxin TRX-h5 leading to enhanced CC susceptibility to fungal infection (e.g. B.cinerea and CC A.brassicicola). Altered RNA polymerase II occupancy and histone CC H3 lysine tri-methylation (H3K36me3) of target genes. CC Insensitivity to abscisic acid (ABA) and to the ethylene precursor CC 1-aminocyclopropane-1-carboxylic acid (ACC). Delayed flowering. CC {ECO:0000269|PubMed:24451981}. CC -!- SIMILARITY: Belongs to the Mediator complex subunit 18 family. CC {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AC006592; AAD22361.1; -; Genomic_DNA. DR EMBL; CP002685; AEC07298.1; -; Genomic_DNA. DR EMBL; BT029378; ABK32192.1; -; mRNA. DR PIR; H84611; H84611. DR RefSeq; NP_565534.1; NM_127802.4. DR UniGene; At.28136; -. DR ProteinModelPortal; Q9SJZ6; -. DR SMR; Q9SJZ6; -. DR BioGrid; 2122; 2. DR IntAct; Q9SJZ6; 1. DR STRING; 3702.AT2G22370.1; -. DR PaxDb; Q9SJZ6; -. DR EnsemblPlants; AT2G22370.1; AT2G22370.1; AT2G22370. DR GeneID; 816769; -. DR Gramene; AT2G22370.1; AT2G22370.1; AT2G22370. DR KEGG; ath:AT2G22370; -. DR Araport; AT2G22370; -. DR TAIR; locus:2041243; AT2G22370. DR eggNOG; ENOG410ISJX; Eukaryota. DR eggNOG; ENOG410ZUU9; LUCA. DR HOGENOM; HOG000005666; -. DR InParanoid; Q9SJZ6; -. DR KO; K15135; -. DR OMA; EPTWTVK; -. DR OrthoDB; EOG09360N9S; -. DR PhylomeDB; Q9SJZ6; -. DR PRO; PR:Q9SJZ6; -. DR Proteomes; UP000006548; Chromosome 2. DR ExpressionAtlas; Q9SJZ6; baseline and differential. DR Genevisible; Q9SJZ6; AT. DR GO; GO:0070847; C:core mediator complex; IBA:GO_Central. DR GO; GO:0016592; C:mediator complex; IDA:UniProtKB. DR GO; GO:0001104; F:RNA polymerase II transcription cofactor activity; IBA:GO_Central. DR GO; GO:0048440; P:carpel development; IGI:TAIR. DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IMP:TAIR. DR GO; GO:0048441; P:petal development; IGI:TAIR. DR GO; GO:0048510; P:regulation of timing of transition from vegetative to reproductive phase; IMP:TAIR. DR GO; GO:0010219; P:regulation of vernalization response; IMP:TAIR. DR GO; GO:0048442; P:sepal development; IGI:TAIR. DR GO; GO:0048833; P:specification of floral organ number; IMP:TAIR. DR GO; GO:0048443; P:stamen development; IGI:TAIR. DR GO; GO:0006369; P:termination of RNA polymerase II transcription; IBA:GO_Central. PE 1: Evidence at protein level; KW Abscisic acid signaling pathway; Complete proteome; KW Developmental protein; Ethylene signaling pathway; Nucleus; KW Plant defense; Reference proteome; Transcription; KW Transcription regulation; Transcription termination. FT CHAIN 1 219 Mediator of RNA polymerase II FT transcription subunit 18. FT /FTId=PRO_0000418352. FT COMPBIAS 203 208 Poly-Ala. {ECO:0000255}. SQ SEQUENCE 219 AA; 23365 MW; FE53D1C081F36F55 CRC64; MSMECVVQGI IETQHVEALE ILLQGLCGVQ RERLRVHELC LRSGPNLGVV SSEVRLLCDL DQPEPTWTVK HVGGAMRGAG ADQISVLVRN MIESKVSKNA LRMFYALGYK LDHELLKVGF AFHFQRTAHI SVSVSSVNKM PKVHAIDEAV PVTPGMQIVD VTAPATSENY SEVAAAVSSF CEFLAPLVHL SKPSISTGVV PTAAAAAASL MSDGGGTTL //