ID MED18_ARATH Reviewed; 219 AA. AC Q9SJZ6; DT 11-JUL-2012, integrated into UniProtKB/Swiss-Prot. DT 01-MAY-2000, sequence version 1. DT 13-SEP-2023, entry version 111. DE RecName: Full=Mediator of RNA polymerase II transcription subunit 18 {ECO:0000303|PubMed:17560376}; GN Name=MED18 {ECO:0000303|PubMed:17560376}; GN Synonyms=MED18_1 {ECO:0000303|PubMed:22021418}; GN OrderedLocusNames=At2g22370 {ECO:0000312|Araport:AT2G22370}; GN ORFNames=F14M13.23 {ECO:0000312|EMBL:AAD22361.1}; OS Arabidopsis thaliana (Mouse-ear cress). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis. OX NCBI_TaxID=3702; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Columbia; RX PubMed=10617197; DOI=10.1038/45471; RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.; RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."; RL Nature 402:761-768(1999). RN [2] RP GENOME REANNOTATION. RC STRAIN=cv. Columbia; RX PubMed=27862469; DOI=10.1111/tpj.13415; RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S., RA Town C.D.; RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference RT genome."; RL Plant J. 89:789-804(2017). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RA Bautista V.R., Kim C.J., Chen H., Quinitio C., Ecker J.R.; RT "Arabidopsis ORF clones."; RL Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases. RN [4] RP IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT, AND NOMENCLATURE. RX PubMed=17560376; DOI=10.1016/j.molcel.2007.05.007; RA Baeckstroem S., Elfving N., Nilsson R., Wingsle G., Bjoerklund S.; RT "Purification of a plant mediator from Arabidopsis thaliana identifies PFT1 RT as the Med25 subunit."; RL Mol. Cell 26:717-729(2007). RN [5] RP IDENTIFICATION, AND NOMENCLATURE. RX PubMed=22021418; DOI=10.1104/pp.111.188300; RA Mathur S., Vyas S., Kapoor S., Tyagi A.K.; RT "The Mediator complex in plants: structure, phylogeny, and expression RT profiling of representative genes in a dicot (Arabidopsis) and a monocot RT (rice) during reproduction and abiotic stress."; RL Plant Physiol. 157:1609-1627(2011). RN [6] RP FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH YY1; ABI4 AND SUF4, AND RP SUBCELLULAR LOCATION. RC STRAIN=cv. Columbia; RX PubMed=24451981; DOI=10.1038/ncomms4064; RA Lai Z., Schluttenhofer C.M., Bhide K., Shreve J., Thimmapuram J., Lee S.Y., RA Yun D.-J., Mengiste T.; RT "MED18 interaction with distinct transcription factors regulates multiple RT plant functions."; RL Nat. Commun. 5:3064-3064(2014). CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in CC the regulated transcription of nearly all RNA polymerase II-dependent CC genes. Mediator functions as a bridge to convey information from gene- CC specific regulatory proteins to the basal RNA polymerase II CC transcription machinery. The Mediator complex, having a compact CC conformation in its free form, is recruited to promoters by direct CC interactions with regulatory proteins and serves for the assembly of a CC functional pre-initiation complex with RNA polymerase II and the CC general transcription factors (PubMed:22021418, PubMed:24451981). CC Involved in the regulation of histone H3 lysine tri-methylation CC (H3K36me3). Associates with the promoter, coding and terminator regions CC of target genes suggesting its function in transcription initiation, CC elongation and termination. Multifunctional protein which regulates CC plant immunity, especially during necrotrophic fungal infection (e.g. CC B.cinerea and A.brassicicola), flowering time and responses to hormones CC (e.g. abscisic acid ABA and ethylene) through interactions with CC distinct transcription factors (PubMed:24451981). CC {ECO:0000269|PubMed:22021418, ECO:0000269|PubMed:24451981}. CC -!- SUBUNIT: Component of the Mediator complex (PubMed:17560376). Interacts CC with YY1 to suppress disease susceptibility via the repression of genes CC glutaredoxins GRX480, GRXS13 and thioredoxin TRX-h5. Binds to ABI4 to CC regulate abscisic acid responses; recruited by ABI4 to ABI5 promoter in CC the presence of abscisic acid (ABA). Interacts with SUF4 to regulate CC flowering time; recruited by SUF4 to FLC promoter (PubMed:24451981). CC {ECO:0000269|PubMed:17560376, ECO:0000269|PubMed:24451981}. CC -!- INTERACTION: CC Q9SJZ6; Q6NPF4: MED20A; NbExp=3; IntAct=EBI-1386265, EBI-1386270; CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:24451981}. CC -!- DISRUPTION PHENOTYPE: Deregulated expression of glutaredoxins GRX480, CC GRXS13 and thioredoxin TRX-h5 leading to enhanced susceptibility to CC fungal infection (e.g. B.cinerea and A.brassicicola). Altered RNA CC polymerase II occupancy and histone H3 lysine tri-methylation CC (H3K36me3) of target genes. Insensitivity to abscisic acid (ABA) and to CC the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC). CC Delayed flowering. {ECO:0000269|PubMed:24451981}. CC -!- SIMILARITY: Belongs to the Mediator complex subunit 18 family. CC {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AC006592; AAD22361.1; -; Genomic_DNA. DR EMBL; CP002685; AEC07298.1; -; Genomic_DNA. DR EMBL; BT029378; ABK32192.1; -; mRNA. DR PIR; H84611; H84611. DR RefSeq; NP_565534.1; NM_127802.4. DR AlphaFoldDB; Q9SJZ6; -. DR SMR; Q9SJZ6; -. DR BioGRID; 2122; 2. DR IntAct; Q9SJZ6; 3. DR STRING; 3702.AT2G22370.1; -. DR PaxDb; Q9SJZ6; -. DR ProteomicsDB; 239052; -. DR EnsemblPlants; AT2G22370.1; AT2G22370.1; AT2G22370. DR GeneID; 816769; -. DR Gramene; AT2G22370.1; AT2G22370.1; AT2G22370. DR KEGG; ath:AT2G22370; -. DR Araport; AT2G22370; -. DR TAIR; AT2G22370; MED18. DR eggNOG; KOG3264; Eukaryota. DR HOGENOM; CLU_111349_0_0_1; -. DR InParanoid; Q9SJZ6; -. DR OMA; PKVHAID; -. DR OrthoDB; 1209867at2759; -. DR PhylomeDB; Q9SJZ6; -. DR PRO; PR:Q9SJZ6; -. DR Proteomes; UP000006548; Chromosome 2. DR ExpressionAtlas; Q9SJZ6; baseline and differential. DR Genevisible; Q9SJZ6; AT. DR GO; GO:0070847; C:core mediator complex; IBA:GO_Central. DR GO; GO:0016592; C:mediator complex; IDA:UniProtKB. DR GO; GO:0005634; C:nucleus; IDA:UniProtKB. DR GO; GO:0003712; F:transcription coregulator activity; IMP:UniProtKB. DR GO; GO:0009738; P:abscisic acid-activated signaling pathway; IEA:UniProtKB-KW. DR GO; GO:0048440; P:carpel development; IGI:TAIR. DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW. DR GO; GO:0009873; P:ethylene-activated signaling pathway; IEA:UniProtKB-KW. DR GO; GO:0035196; P:miRNA processing; IMP:TAIR. DR GO; GO:0045892; P:negative regulation of DNA-templated transcription; IMP:TAIR. DR GO; GO:0048441; P:petal development; IGI:TAIR. DR GO; GO:0060261; P:positive regulation of transcription initiation by RNA polymerase II; IMP:UniProtKB. DR GO; GO:1900150; P:regulation of defense response to fungus; IMP:UniProtKB. DR GO; GO:0006355; P:regulation of DNA-templated transcription; IMP:UniProtKB. DR GO; GO:0032784; P:regulation of DNA-templated transcription elongation; IDA:UniProtKB. DR GO; GO:2000142; P:regulation of DNA-templated transcription initiation; IDA:UniProtKB. DR GO; GO:2000028; P:regulation of photoperiodism, flowering; IMP:UniProtKB. DR GO; GO:2000031; P:regulation of salicylic acid mediated signaling pathway; IMP:TAIR. DR GO; GO:0031554; P:regulation of termination of DNA-templated transcription; IDA:UniProtKB. DR GO; GO:0048510; P:regulation of timing of transition from vegetative to reproductive phase; IMP:TAIR. DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central. DR GO; GO:0010219; P:regulation of vernalization response; IMP:TAIR. DR GO; GO:0009737; P:response to abscisic acid; IMP:UniProtKB. DR GO; GO:0009723; P:response to ethylene; IMP:UniProtKB. DR GO; GO:0048442; P:sepal development; IGI:TAIR. DR GO; GO:0048833; P:specification of floral organ number; IMP:TAIR. DR GO; GO:0048443; P:stamen development; IGI:TAIR. DR GO; GO:0006369; P:termination of RNA polymerase II transcription; IBA:GO_Central. DR Gene3D; 2.40.320.10; Hypothetical Protein Pfu-838710-001; 1. DR InterPro; IPR019095; Mediator_Med18. DR PANTHER; PTHR13321:SF2; MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 18; 1. DR PANTHER; PTHR13321; MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION, SUBUNIT 18; 1. PE 1: Evidence at protein level; KW Abscisic acid signaling pathway; Developmental protein; KW Ethylene signaling pathway; Nucleus; Plant defense; Reference proteome; KW Transcription; Transcription regulation; Transcription termination. FT CHAIN 1..219 FT /note="Mediator of RNA polymerase II transcription subunit FT 18" FT /id="PRO_0000418352" SQ SEQUENCE 219 AA; 23365 MW; FE53D1C081F36F55 CRC64; MSMECVVQGI IETQHVEALE ILLQGLCGVQ RERLRVHELC LRSGPNLGVV SSEVRLLCDL DQPEPTWTVK HVGGAMRGAG ADQISVLVRN MIESKVSKNA LRMFYALGYK LDHELLKVGF AFHFQRTAHI SVSVSSVNKM PKVHAIDEAV PVTPGMQIVD VTAPATSENY SEVAAAVSSF CEFLAPLVHL SKPSISTGVV PTAAAAAASL MSDGGGTTL //