ID I2C2_RAT STANDARD; PRT; 860 AA. AC Q9QZ81; DT 16-OCT-2001 (Rel. 40, Created) DT 29-MAR-2004 (Rel. 43, Last sequence update) DT 13-SEP-2005 (Rel. 48, Last annotation update) DE Eukaryotic translation initiation factor 2C 2 (eIF2C 2) (eIF-2C 2) DE (Argonaute-2) (Golgi ER protein 95 kDa) (GERp95). GN Name=Eif2c2; Synonyms=Ago2; OS Rattus norvegicus (Rat). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; OC Muroidea; Muridae; Murinae; Rattus. OX NCBI_TaxID=10116; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RC STRAIN=Sprague-Dawley; TISSUE=Hepatoma; RX MEDLINE=99443791; PubMed=10512872; RA Cikaluk D.E., Tahbaz N., Hendricks L.C., DiMattia G.E., Hansen D., RA Pilgrim D., Hobman T.C.; RT "GERp95, a membrane-associated protein that belongs to a family of RT proteins involved in stem cell differentiation."; RL Mol. Biol. Cell 10:3357-3372(1999). CC -!- FUNCTION: Provides endonuclease activity to RNA-induced silencing CC complexes (RISC). Cleaves siRNA/mRNA heteroduplexes bound to RISC. CC Essential for embryonic development as well as RNA-mediated gene CC silencing (RNAi) (By similarity). CC -!- SUBUNIT: Interacts with DICER1 through its Piwi domain (By CC similarity). CC -!- SUBCELLULAR LOCATION: Cytoplasmic bodies (By similarity). CC -!- DOMAIN: Piwi domain is thought to provide RNA cleavage activity CC with a mechanism similar to RNase H (By similarity). CC -!- SIMILARITY: Belongs to the argonaute family. CC -!- SIMILARITY: Contains 1 PAZ domain. CC -!- SIMILARITY: Contains 1 Piwi domain. CC -!- CAUTION: Was originally (Ref.1) thought to be membrane-associated. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AF195534; AAF12800.1; ALT_INIT; mRNA. DR SMR; Q9QZ81; 223-390. DR Ensembl; ENSRNOG00000008533; Rattus norvegicus. DR RGD; 621255; Eif2c2. DR GO; GO:0030154; P:cell differentiation; NAS. DR InterPro; IPR003100; PAZ. DR InterPro; IPR003165; Piwi. DR Pfam; PF02170; PAZ; 1. DR Pfam; PF02171; Piwi; 1. DR PROSITE; PS50821; PAZ; 1. DR PROSITE; PS50822; PIWI; 1. KW Initiation factor; Protein biosynthesis. FT DOMAIN 236 349 PAZ. FT DOMAIN 518 819 Piwi. SQ SEQUENCE 860 AA; 97318 MW; A5B0798C66481C9C CRC64; MYSGAGPVLA SPAPTTSPIP GYAFKPPPRP DFGTTGRTIK LQANFFEMDI PKIDIYHYEL DIKPEKCPRR VNREIVEHMV QHFKTQIFGD RKPVFDGRKN LYTAMPLPIG RDKVELEVTL PGEGKDRIFK VSIKWVSCVS LQALHDALSG RLPSVPFETI QALDVVMRHL PSMRYTPVGR SFFTASEGCS NPLGGGREVW FGFHQSVRPS LWKMMLNIDV SATAFYKAQP VIEFVCEVLD FKSIEEQQKP LTDSQRVKFT KEIKGLKVEI THCGQMKRKY RVCNVTRRPA SHQTFPLQQE SGQTVECTVA QYFKDRHKLV LRYPHLPCLQ VGQEQKHTYL PLEVCNIVAG QRCIKKLTDN QTSTMIRATA RSAPDRQEEI SKLMRSASFN TDPYVREFGI MVKDEMTDVT GRVLQPPSIL YGGRNKAIAT PVQGVWDMRN KQFHTGIEIK VWAIACFAPQ RQCTEVHLKS FTEQLRKISR DAGMPIQGQP CFCKYAQGAD SVEPMFRHLK NTYAGLQLVV VILPGKTPVY AEVKRVGDTV LGMATQCVQM KNVQRTTPQT LSNLCLKINV KLGGVNNILL PQGRPPVFQQ PVIFLGADVT HPPAGDGKKP SIAAVVGSMD AHPNRYCATV RVQQHRQEII QDLAAMVREL LIQFYKSTRF KPTRIIFYRD GVSEGQFQQV LHHELLAIRE ACIKLEKEYQ PGITFIVVQK RHHTRLFCTD KNERVGKSGN IPAGTTVDTK ITHPTEFDFY LCSHAGIQGT SRPSHYHVLW DDNRFSSDEL QILTYQLCHT YVRCTRSVSI PAPAYYAHLV AFRARYHLVD KEHDSAEGSH TSGQSNGRDH QALAKAVQVH QDTLRTMYFA //