ID SPY2_MOUSE Reviewed; 315 AA.
AC Q9QXV8; Q9WUQ9;
DT 21-FEB-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 22-JAN-2014, entry version 99.
DE RecName: Full=Protein sprouty homolog 2;
DE Short=Spry-2;
GN Name=Spry2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC Muroidea; Muridae; Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=10498682;
RA Minowada G., Jarvis L.A., Chi C.L., Neubueser A., Sun X., Hacohen N.,
RA Krasnow M.A., Martin G.R.;
RT "Vertebrate sprouty genes are induced by FGF signaling and can cause
RT chondrodysplasia when overexpressed.";
RL Development 126:4465-4475(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Swiss Webster;
RX PubMed=10074434; DOI=10.1016/S0960-9822(99)80094-3;
RA Tefft J.D., Lee M., Smith S., Leinwand M., Zhao J., Bringas P. Jr.,
RA Crowe D.L., Warburton D.;
RT "Conserved function of mSpry-2, a murine homolog of Drosophila
RT sprouty, which negatively modulates respiratory organogenesis.";
RL Curr. Biol. 9:219-222(1999).
CC -!- FUNCTION: May function as an antagonist of fibroblast growth
CC factor (FGF) pathways and may negatively modulate respiratory
CC organogenesis.
CC -!- SUBUNIT: Interacts with RAF1 (By similarity).
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. Cell projection,
CC ruffle membrane. Note=Associated with microtubules in unstimulated
CC cells but is translocated to the membrane ruffles in cells
CC stimulated ith EGF (epidermal growth factor).
CC -!- TISSUE SPECIFICITY: In adult, highly expressed in brain, lung,
CC heart and at lower levels in skeletal muscle and kidney. In
CC embryo, highly expressed in lung epithelial cells, primarily in
CC the distal airways.
CC -!- DEVELOPMENTAL STAGE: At E8.5 expressed in the primitive streak,
CC rostral forebrain, cells lateral to the posterior hindbrain,
CC anterior hindbrain and developing midbrain. At E9.5 continues to
CC be expressed in the rostral forebrain and primitive streak, and is
CC also detected in the branchial arches and the forelimb bud. At
CC E10.5 expressed in the somites, frontonasal processes, tailbud,
CC and hindlimb bud.
CC -!- INDUCTION: By FGF signaling.
CC -!- DOMAIN: The Cys-rich domain is responsible for the localization of
CC the protein to the membrane ruffles.
CC -!- SIMILARITY: Belongs to the sprouty family.
CC -!- SIMILARITY: Contains 1 SPR (sprouty) domain.
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DR EMBL; AF176905; AAD56006.1; -; mRNA.
DR EMBL; AF153084; AAD34167.1; -; mRNA.
DR RefSeq; NP_036027.1; NM_011897.3.
DR UniGene; Mm.489870; -.
DR UniGene; Mm.89982; -.
DR ProteinModelPortal; Q9QXV8; -.
DR IntAct; Q9QXV8; 1.
DR MINT; MINT-1591537; -.
DR PhosphoSite; Q9QXV8; -.
DR PRIDE; Q9QXV8; -.
DR Ensembl; ENSMUST00000022709; ENSMUSP00000022709; ENSMUSG00000022114.
DR GeneID; 24064; -.
DR KEGG; mmu:24064; -.
DR UCSC; uc007uxy.2; mouse.
DR CTD; 10253; -.
DR MGI; MGI:1345138; Spry2.
DR eggNOG; NOG83905; -.
DR GeneTree; ENSGT00390000003535; -.
DR HOGENOM; HOG000233079; -.
DR HOVERGEN; HBG003544; -.
DR InParanoid; Q9QXV8; -.
DR KO; K17383; -.
DR OMA; ETRAQNG; -.
DR OrthoDB; EOG7G4QDV; -.
DR NextBio; 304033; -.
DR PRO; PR:Q9QXV8; -.
DR ArrayExpress; Q9QXV8; -.
DR Bgee; Q9QXV8; -.
DR CleanEx; MM_SPRY2; -.
DR Genevestigator; Q9QXV8; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0032587; C:ruffle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0043539; F:protein serine/threonine kinase activator activity; IEA:Ensembl.
DR GO; GO:0060449; P:bud elongation involved in lung branching; IGI:MGI.
DR GO; GO:0045165; P:cell fate commitment; IMP:MGI.
DR GO; GO:0000132; P:establishment of mitotic spindle orientation; IGI:MGI.
DR GO; GO:0042472; P:inner ear morphogenesis; IMP:MGI.
DR GO; GO:0060437; P:lung growth; IDA:MGI.
DR GO; GO:0043066; P:negative regulation of apoptotic process; IMP:UniProtKB.
DR GO; GO:0008285; P:negative regulation of cell proliferation; IDA:MGI.
DR GO; GO:0070373; P:negative regulation of ERK1 and ERK2 cascade; IDA:MGI.
DR GO; GO:0040037; P:negative regulation of fibroblast growth factor receptor signaling pathway; IDA:MGI.
DR GO; GO:0043407; P:negative regulation of MAP kinase activity; IDA:MGI.
DR GO; GO:0051387; P:negative regulation of neurotrophin TRK receptor signaling pathway; IDA:MGI.
DR GO; GO:0010801; P:negative regulation of peptidyl-threonine phosphorylation; IEA:Ensembl.
DR GO; GO:0034261; P:negative regulation of Ras GTPase activity; IDA:MGI.
DR GO; GO:0046580; P:negative regulation of Ras protein signal transduction; IDA:MGI.
DR GO; GO:0070374; P:positive regulation of ERK1 and ERK2 cascade; IEA:Ensembl.
DR GO; GO:0010628; P:positive regulation of gene expression; IEA:Ensembl.
DR GO; GO:0033138; P:positive regulation of peptidyl-serine phosphorylation; IEA:Ensembl.
DR GO; GO:0051897; P:positive regulation of protein kinase B signaling cascade; IEA:Ensembl.
DR GO; GO:0007605; P:sensory perception of sound; IMP:MGI.
DR InterPro; IPR007875; Sprouty.
DR Pfam; PF05210; Sprouty; 1.
DR PROSITE; PS51227; SPR; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Cell projection; Complete proteome; Cytoplasm;
KW Cytoskeleton; Developmental protein; Membrane; Microtubule;
KW Reference proteome.
FT CHAIN 1 315 Protein sprouty homolog 2.
FT /FTId=PRO_0000076902.
FT DOMAIN 177 291 SPR.
FT COMPBIAS 124 130 Poly-Ser.
FT COMPBIAS 178 301 Cys-rich.
FT CONFLICT 163 163 V -> I (in Ref. 2; AAD34167).
SQ SEQUENCE 315 AA; 34623 MW; 81514698EAD809A7 CRC64;
MEARAQSGNG SQPLLQTAHD SGRQRGEPDP RDALTQQVHV LSLDQIRAIR NTNEYTEGPT
VVPRPGLKPA PRPSTQHKHE RLHGLPEHRQ PPRLQPSQVH SSRAPLSRSI STVSSGSRSS
TRTSTSSSSS EQRLLGPSFS HGPAAADGII RVQPKSELKP GDVKPLSKDD LGLHAYRCED
CGKCKCKECT YPRPLPSDWI CDKQCLCSAQ NVIDYGTCVC CVKGLFYHCS NDDEDNCADN
PCSCSQSHCC TRWSAMGVMS LFLPCLWCYL PAKGCLKLCQ GCYDRVNRPG CRCKNSNTVC
CKVPTVPPRN FEKPT
//