ID LEXA_XYLFA STANDARD; PRT; 211 AA. AC Q9PH24; DT 01-OCT-2000 (Rel. 40, Created) DT 01-OCT-2000 (Rel. 40, Last sequence update) DT 01-OCT-2000 (Rel. 40, Last annotation update) DE LEXA REPRESSOR (EC 3.4.21.88). GN LEXA OR XF0122. OS Xylella fastidiosa. OC Bacteria; Proteobacteria; gamma subdivision; Xanthomonas group; OC Xylella. OX NCBI_TaxID=2371; RN [1] RP SEQUENCE FROM N.A. RC STRAIN=9A5C; RX MEDLINE=20365717; PubMed=10910347; RA Simpson A.J.G., Reinach F.C., Arruda P., Abreu F.A., Acencio M., RA Alvarenga R., Alves L.M.C., Araya J.E., Baia G.S., Baptista C.S., RA Barros M.H., Bonaccorsi E.D., Bordin S., Bove J.M., Briones M.R.S., RA Bueno M.R.P., Camargo A.A., Camargo L.E.A., Carraro D.M., Carrer H., RA Colauto N.B., Colombo C., Costa F.F., Costa M.C.R., Costa-Neto C.M., RA Coutinho L.L., Cristofani M., Dias-Neto E., Docena C., El-Dorry H., RA Facincani A.P., Ferreira A.J.S., Ferreira V.C.A., Ferro J.A., RA Fraga J.S., Franca S.C., Franco M.C., Frohme M., Furlan L.R., RA Garnier M., Goldman G.H., Goldman M.H.S., Gomes S.L., Gruber A., RA Ho P.L., Hoheisel J.D., Junqueira M.L., Kemper E.L., Kitajima J.P., RA Krieger J.E., Kuramae E.E., Laigret F., Lambais M.R., Leite L.C.C., RA Lemos E.G.M., Lemos M.V.F., Lopes S.A., Lopes C.R., Machado J.A., RA Machado M.A., Madeira A.M.B.N., Madeira H.M.F., Marino C.L., RA Marques M.V., Martins E.A.L., Martins E.M.F., Matsukuma A.Y., RA Menck C.F.M., Miracca E.C., Miyaki C.Y., Monteiro-Vitorello C.B., RA Moon D.H., Nagai M.A., Nascimento A.L.T.O., Netto L.E.S., RA Nhani A. Jr., Nobrega F.G., Nunes L.R., Oliveira M.A., RA de Oliveira M.C., de Oliveira R.C., Palmieri D.A., Paris A., RA Peixoto B.R., Pereira G.A.G., Pereira H.A. Jr., Pesquero J.B., RA Quaggio R.B., Roberto P.G., Rodrigues V., de Rosa A.J.M., RA de Rosa V.E. Jr., de Sa R.G., Santelli R.V., Sawasaki H.E., RA da Silva A.C.R., da Silva A.M., da Silva F.R., Silva W.A. Jr., RA da Silveira J.F., Silvestri M.L.Z., Siqueira W.J., de Souza A.A., RA de Souza A.P., Terenzi M.F., Truffi D., Tsai S.M., Tsuhako M.H., RA Vallada H., Van Sluys M.A., Verjovski-Almeida S., Vettore A.L., RA Zago M.A., Zatz M., Meidanis J., Setubal J.C.; RT "The genome sequence of the plant pathogen Xylella fastidiosa."; RL Nature 406:151-159(2000). CC -!- FUNCTION: REPRESSES A NUMBER OF GENES INVOLVED IN THE RESPONSE TO CC DNA DAMAGE (SOS RESPONSE), INCLUDING RECA AND LEXA. BINDS TO THE CC 16 BP PALINDROMIC SEQUENCE 5'-CTGT-AT(4)-ACAG-3'(BY SIMILARITY). CC IN THE PRESENCE OF SINGLE-STRANDED DNA, RECA INTERACTS WITH LEXA CC CAUSING AN AUTOCATALYTIC CLEAVAGE WHICH DISRUPTS THE DNA-BINDING CC PART OF LEXA, LEADING TO DEREPRESSION OF THE SOS REGULON AND CC EVENTUALLY DNA REPAIR (BY SIMILARITY). CC -!- CATALYTIC ACTIVITY: HYDROLYSIS OF ALA-|-GLY BOND IN REPRESSOR CC LEXA. CC -!- SUBUNIT: HOMODIMER (BY SIMILARITY). CC -!- SIMILARITY: BELONGS TO PEPTIDASE FAMILY S24 ALSO KNOWN AS THE CC UMUD/LEXA FAMILY. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AE003865; AAF82935.1; ALT_INIT. DR MEROPS; S24.001; -. DR InterPro; IPR002865; LexA_DNA_bind. DR InterPro; IPR000129; Peptidase_S24. DR Pfam; PF01726; LexA_DNA_bind; 1. DR Pfam; PF00717; Peptidase_S24; 1. DR PRINTS; PR00726; LEXASERPTASE. KW Transcription regulation; Repressor; DNA-binding; DNA damage; KW Autocatalytic cleavage; Hydrolase; DNA replication; SOS response. FT DNA_BIND 27 47 H-T-H MOTIF (BY SIMILARITY). FT SITE 96 97 CLEAVAGE (AUTO-) (BY SIMILARITY). FT ACT_SITE 131 131 INVOLVED IN AUTO-CLEAVAGE (BY FT SIMILARITY). FT ACT_SITE 168 168 INVOLVED IN AUTO-CLEAVAGE (BY FT SIMILARITY). SQ SEQUENCE 211 AA; 23395 MW; 3BB6A0560AD62571 CRC64; MSLSDIQQAI LSLITNHINA DGVSPSQTEI ARAFGFKGVR AVQHHLDVLE QQGMIRRVPR QARGIRLKHL TEVDETALAL QSEDVLRLPV LGRVAAGQPI GADIGEGRVV LLDRVFFSPA PDYLLRVQGD SMRDEGIFDG DLIGVHRTQD AHSGQIVVAR IDDEITVKLL KISKDRIRLL PRNPDFAPIE VRSDQDFAIE GLYCGLLRPN R //