ID LEXA_XYLFA Reviewed; 211 AA. AC Q9PH24; DT 27-APR-2001, integrated into UniProtKB/Swiss-Prot. DT 27-APR-2001, sequence version 2. DT 22-FEB-2023, entry version 132. DE RecName: Full=LexA repressor {ECO:0000255|HAMAP-Rule:MF_00015}; DE EC=3.4.21.88 {ECO:0000255|HAMAP-Rule:MF_00015}; GN Name=lexA {ECO:0000255|HAMAP-Rule:MF_00015}; OrderedLocusNames=XF_0122; OS Xylella fastidiosa (strain 9a5c). OC Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; OC Xanthomonadaceae; Xylella. OX NCBI_TaxID=160492; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=9a5c; RX PubMed=10910347; DOI=10.1038/35018003; RA Simpson A.J.G., Reinach F.C., Arruda P., Abreu F.A., Acencio M., RA Alvarenga R., Alves L.M.C., Araya J.E., Baia G.S., Baptista C.S., RA Barros M.H., Bonaccorsi E.D., Bordin S., Bove J.M., Briones M.R.S., RA Bueno M.R.P., Camargo A.A., Camargo L.E.A., Carraro D.M., Carrer H., RA Colauto N.B., Colombo C., Costa F.F., Costa M.C.R., Costa-Neto C.M., RA Coutinho L.L., Cristofani M., Dias-Neto E., Docena C., El-Dorry H., RA Facincani A.P., Ferreira A.J.S., Ferreira V.C.A., Ferro J.A., Fraga J.S., RA Franca S.C., Franco M.C., Frohme M., Furlan L.R., Garnier M., Goldman G.H., RA Goldman M.H.S., Gomes S.L., Gruber A., Ho P.L., Hoheisel J.D., RA Junqueira M.L., Kemper E.L., Kitajima J.P., Krieger J.E., Kuramae E.E., RA Laigret F., Lambais M.R., Leite L.C.C., Lemos E.G.M., Lemos M.V.F., RA Lopes S.A., Lopes C.R., Machado J.A., Machado M.A., Madeira A.M.B.N., RA Madeira H.M.F., Marino C.L., Marques M.V., Martins E.A.L., Martins E.M.F., RA Matsukuma A.Y., Menck C.F.M., Miracca E.C., Miyaki C.Y., RA Monteiro-Vitorello C.B., Moon D.H., Nagai M.A., Nascimento A.L.T.O., RA Netto L.E.S., Nhani A. Jr., Nobrega F.G., Nunes L.R., Oliveira M.A., RA de Oliveira M.C., de Oliveira R.C., Palmieri D.A., Paris A., Peixoto B.R., RA Pereira G.A.G., Pereira H.A. Jr., Pesquero J.B., Quaggio R.B., RA Roberto P.G., Rodrigues V., de Rosa A.J.M., de Rosa V.E. Jr., de Sa R.G., RA Santelli R.V., Sawasaki H.E., da Silva A.C.R., da Silva A.M., RA da Silva F.R., Silva W.A. Jr., da Silveira J.F., Silvestri M.L.Z., RA Siqueira W.J., de Souza A.A., de Souza A.P., Terenzi M.F., Truffi D., RA Tsai S.M., Tsuhako M.H., Vallada H., Van Sluys M.A., Verjovski-Almeida S., RA Vettore A.L., Zago M.A., Zatz M., Meidanis J., Setubal J.C.; RT "The genome sequence of the plant pathogen Xylella fastidiosa."; RL Nature 406:151-159(2000). CC -!- FUNCTION: Represses a number of genes involved in the response to DNA CC damage (SOS response), including recA and lexA. In the presence of CC single-stranded DNA, RecA interacts with LexA causing an autocatalytic CC cleavage which disrupts the DNA-binding part of LexA, leading to CC derepression of the SOS regulon and eventually DNA repair. CC {ECO:0000255|HAMAP-Rule:MF_00015}. CC -!- CATALYTIC ACTIVITY: CC Reaction=Hydrolysis of Ala-|-Gly bond in repressor LexA.; EC=3.4.21.88; CC Evidence={ECO:0000255|HAMAP-Rule:MF_00015}; CC -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00015}. CC -!- SIMILARITY: Belongs to the peptidase S24 family. {ECO:0000255|HAMAP- CC Rule:MF_00015}. CC -!- SEQUENCE CAUTION: CC Sequence=AAF82935.1; Type=Erroneous initiation; Evidence={ECO:0000305}; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AE003849; AAF82935.1; ALT_INIT; Genomic_DNA. DR PIR; H82846; H82846. DR RefSeq; WP_023906043.1; NC_002488.3. DR AlphaFoldDB; Q9PH24; -. DR SMR; Q9PH24; -. DR STRING; 160492.XF_0122; -. DR MEROPS; S24.001; -. DR EnsemblBacteria; AAF82935; AAF82935; XF_0122. DR KEGG; xfa:XF_0122; -. DR eggNOG; COG1974; Bacteria. DR HOGENOM; CLU_066192_45_3_6; -. DR OMA; DDEFHIR; -. DR Proteomes; UP000000812; Chromosome. DR CollecTF; EXPREG_000017c0; -. DR GO; GO:0032993; C:protein-DNA complex; IMP:CollecTF. DR GO; GO:0001217; F:DNA-binding transcription repressor activity; IMP:CollecTF. DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:UniProtKB-UniRule. DR GO; GO:0000976; F:transcription cis-regulatory region binding; IMP:CollecTF. DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule. DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-UniRule. DR GO; GO:0045892; P:negative regulation of DNA-templated transcription; EXP:CollecTF. DR GO; GO:0006508; P:proteolysis; IEA:InterPro. DR GO; GO:0009432; P:SOS response; EXP:CollecTF. DR CDD; cd06529; S24_LexA-like; 1. DR Gene3D; 2.10.109.10; Umud Fragment, subunit A; 1. DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1. DR HAMAP; MF_00015; LexA; 1. DR InterPro; IPR006200; LexA. DR InterPro; IPR039418; LexA-like. DR InterPro; IPR036286; LexA/Signal_pep-like_sf. DR InterPro; IPR006199; LexA_DNA-bd_dom. DR InterPro; IPR006197; Peptidase_S24_LexA. DR InterPro; IPR015927; Peptidase_S24_S26A/B/C. DR InterPro; IPR036388; WH-like_DNA-bd_sf. DR InterPro; IPR036390; WH_DNA-bd_sf. DR PANTHER; PTHR33516; LEXA REPRESSOR; 1. DR PANTHER; PTHR33516:SF2; LEXA REPRESSOR; 1. DR Pfam; PF01726; LexA_DNA_bind; 1. DR Pfam; PF00717; Peptidase_S24; 1. DR PRINTS; PR00726; LEXASERPTASE. DR SUPFAM; SSF51306; LexA/Signal peptidase; 1. DR SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1. DR TIGRFAMs; TIGR00498; lexA; 1. PE 3: Inferred from homology; KW Autocatalytic cleavage; DNA damage; DNA repair; DNA replication; KW DNA-binding; Hydrolase; Repressor; SOS response; Transcription; KW Transcription regulation. FT CHAIN 1..211 FT /note="LexA repressor" FT /id="PRO_0000170113" FT DNA_BIND 27..47 FT /note="H-T-H motif" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00015" FT ACT_SITE 131 FT /note="For autocatalytic cleavage activity" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00015" FT ACT_SITE 168 FT /note="For autocatalytic cleavage activity" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00015" FT SITE 96..97 FT /note="Cleavage; by autolysis" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00015" SQ SEQUENCE 211 AA; 23395 MW; 3BB6A0560AD62571 CRC64; MSLSDIQQAI LSLITNHINA DGVSPSQTEI ARAFGFKGVR AVQHHLDVLE QQGMIRRVPR QARGIRLKHL TEVDETALAL QSEDVLRLPV LGRVAAGQPI GADIGEGRVV LLDRVFFSPA PDYLLRVQGD SMRDEGIFDG DLIGVHRTQD AHSGQIVVAR IDDEITVKLL KISKDRIRLL PRNPDFAPIE VRSDQDFAIE GLYCGLLRPN R //