ID   USE1_HUMAN              Reviewed;         259 AA.
AC   Q9NZ43; Q8NCK1; Q9BRT4;
DT   01-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT   18-MAY-2010, sequence version 2.
DT   03-AUG-2022, entry version 155.
DE   RecName: Full=Vesicle transport protein USE1;
DE   AltName: Full=Putative MAPK-activating protein PM26;
DE   AltName: Full=USE1-like protein;
DE   AltName: Full=p31;
GN   Name=USE1; Synonyms=USE1L; ORFNames=MDS032;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT SER-154.
RC   TISSUE=Lung fibroblast;
RX   PubMed=12761501; DOI=10.1038/sj.onc.1206406;
RA   Matsuda A., Suzuki Y., Honda G., Muramatsu S., Matsuzaki O., Nagano Y.,
RA   Doi T., Shimotohno K., Harada T., Nishida E., Hayashi H., Sugano S.;
RT   "Large-scale identification and characterization of human genes that
RT   activate NF-kappaB and MAPK signaling pathways.";
RL   Oncogene 22:3307-3318(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC   TISSUE=Mammary gland;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT SER-154.
RC   TISSUE=Hematopoietic stem cell;
RA   Zhao M., Gu J., Li N., Peng Y., Han Z., Chen Z.;
RT   "Novel genes expressed in hematopoietic stem/progenitor cells from
RT   myelodysplastic syndrome patients.";
RL   Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15057824; DOI=10.1038/nature02399;
RA   Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E.,
RA   Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A.,
RA   Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S.,
RA   Carrano A.V., Caoile C., Chan Y.M., Christensen M., Cleland C.A.,
RA   Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J.,
RA   Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M.,
RA   Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W.,
RA   Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V.,
RA   Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D.,
RA   McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I.,
RA   Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L.,
RA   Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A.,
RA   She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M.,
RA   Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J.,
RA   Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E.,
RA   Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M.,
RA   Rubin E.M., Lucas S.M.;
RT   "The DNA sequence and biology of human chromosome 19.";
RL   Nature 428:529-535(2004).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), AND VARIANT
RP   SER-154.
RC   TISSUE=Brain, and Skeletal muscle;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [6]
RP   PARTIAL PROTEIN SEQUENCE, IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR
RP   LOCATION, IDENTIFICATION IN A COMPLEX WITH STX18 AND SEC22B, AND
RP   INTERACTION WITH STX18.
RX   PubMed=15029241; DOI=10.1038/sj.emboj.7600135;
RA   Hirose H., Arasaki K., Dohmae N., Takio K., Hatsuzawa K., Nagahama M.,
RA   Tani K., Yamamoto A., Tohyama M., Tagaya M.;
RT   "Implication of ZW10 in membrane trafficking between the endoplasmic
RT   reticulum and Golgi.";
RL   EMBO J. 23:1267-1278(2004).
RN   [7]
RP   FUNCTION, AND IDENTIFICATION IN A COMPLEX WITH STX18; BNIP1 AND SEC22B.
RX   PubMed=15272311; DOI=10.1038/sj.emboj.7600333;
RA   Nakajima K., Hirose H., Taniguchi M., Kurashina H., Arasaki K.,
RA   Nagahama M., Tani K., Yamamoto A., Tagaya M.;
RT   "Involvement of BNIP1 in apoptosis and endoplasmic reticulum membrane
RT   fusion.";
RL   EMBO J. 23:3216-3226(2004).
RN   [8]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA   Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA   Bennett K.L., Superti-Furga G., Colinge J.;
RT   "Initial characterization of the human central proteome.";
RL   BMC Syst. Biol. 5:17-17(2011).
RN   [9]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=25944712; DOI=10.1002/pmic.201400617;
RA   Vaca Jacome A.S., Rabilloud T., Schaeffer-Reiss C., Rompais M., Ayoub D.,
RA   Lane L., Bairoch A., Van Dorsselaer A., Carapito C.;
RT   "N-terminome analysis of the human mitochondrial proteome.";
RL   Proteomics 15:2519-2524(2015).
CC   -!- FUNCTION: SNARE that may be involved in targeting and fusion of Golgi-
CC       derived retrograde transport vesicles with the ER.
CC       {ECO:0000269|PubMed:15272311}.
CC   -!- SUBUNIT: Component of a SNARE complex consisting of STX18, USE1L,
CC       BNIP1/SEC20L and SEC22B. Interacts directly with STX18.
CC       {ECO:0000269|PubMed:15029241, ECO:0000269|PubMed:15272311}.
CC   -!- INTERACTION:
CC       Q9NZ43; Q9BQE5: APOL2; NbExp=3; IntAct=EBI-742842, EBI-4290634;
CC       Q9NZ43; Q13520: AQP6; NbExp=3; IntAct=EBI-742842, EBI-13059134;
CC       Q9NZ43; J3KQ12: BSCL2; NbExp=3; IntAct=EBI-742842, EBI-11532900;
CC       Q9NZ43; Q9BXU9: CALN1; NbExp=3; IntAct=EBI-742842, EBI-12187137;
CC       Q9NZ43; Q6NSX1: CCDC70; NbExp=3; IntAct=EBI-742842, EBI-6873045;
CC       Q9NZ43; Q9NUG4: CCM2L; NbExp=3; IntAct=EBI-742842, EBI-350645;
CC       Q9NZ43; Q15125: EBP; NbExp=3; IntAct=EBI-742842, EBI-3915253;
CC       Q9NZ43; Q8N5M9: JAGN1; NbExp=3; IntAct=EBI-742842, EBI-10266796;
CC       Q9NZ43; P50222: MEOX2; NbExp=3; IntAct=EBI-742842, EBI-748397;
CC       Q9NZ43; Q5SR56: MFSD14B; NbExp=3; IntAct=EBI-742842, EBI-373355;
CC       Q9NZ43; Q9NY72: SCN3B; NbExp=3; IntAct=EBI-742842, EBI-17247926;
CC       Q9NZ43; Q9P2W9: STX18; NbExp=8; IntAct=EBI-742842, EBI-725334;
CC       Q9NZ43; Q16623: STX1A; NbExp=3; IntAct=EBI-742842, EBI-712466;
CC       Q9NZ43; Q12846: STX4; NbExp=3; IntAct=EBI-742842, EBI-744942;
CC       Q9NZ43; Q8N661: TMEM86B; NbExp=3; IntAct=EBI-742842, EBI-2548832;
CC       Q9NZ43; P14373: TRIM27; NbExp=3; IntAct=EBI-742842, EBI-719493;
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000269|PubMed:15029241}; Single-pass type IV membrane protein
CC       {ECO:0000269|PubMed:15029241}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=3;
CC       Name=1;
CC         IsoId=Q9NZ43-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q9NZ43-2; Sequence=VSP_012664, VSP_012665;
CC       Name=3;
CC         IsoId=Q9NZ43-3; Sequence=VSP_012662, VSP_012663;
CC   -!- SIMILARITY: Belongs to the USE1 family. {ECO:0000305}.
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DR   EMBL; AB097050; BAC77403.1; -; mRNA.
DR   EMBL; AK074683; BAC11136.1; -; mRNA.
DR   EMBL; AF220052; AAF67645.1; -; mRNA.
DR   EMBL; AC020913; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC006005; AAH06005.1; -; mRNA.
DR   EMBL; BC008455; AAH08455.1; -; mRNA.
DR   CCDS; CCDS46011.1; -. [Q9NZ43-1]
DR   RefSeq; NP_060937.1; NM_018467.3. [Q9NZ43-1]
DR   RefSeq; XP_016882464.1; XM_017026975.1.
DR   AlphaFoldDB; Q9NZ43; -.
DR   SMR; Q9NZ43; -.
DR   BioGRID; 120952; 136.
DR   IntAct; Q9NZ43; 79.
DR   MINT; Q9NZ43; -.
DR   STRING; 9606.ENSP00000263897; -.
DR   iPTMnet; Q9NZ43; -.
DR   MetOSite; Q9NZ43; -.
DR   PhosphoSitePlus; Q9NZ43; -.
DR   BioMuta; USE1; -.
DR   DMDM; 296452858; -.
DR   EPD; Q9NZ43; -.
DR   jPOST; Q9NZ43; -.
DR   MassIVE; Q9NZ43; -.
DR   MaxQB; Q9NZ43; -.
DR   PaxDb; Q9NZ43; -.
DR   PeptideAtlas; Q9NZ43; -.
DR   PRIDE; Q9NZ43; -.
DR   ProteomicsDB; 83320; -. [Q9NZ43-1]
DR   ProteomicsDB; 83321; -. [Q9NZ43-2]
DR   ProteomicsDB; 83322; -. [Q9NZ43-3]
DR   Antibodypedia; 7758; 225 antibodies from 27 providers.
DR   DNASU; 55850; -.
DR   Ensembl; ENST00000263897.10; ENSP00000263897.4; ENSG00000053501.14. [Q9NZ43-1]
DR   Ensembl; ENST00000595101.5; ENSP00000472362.1; ENSG00000053501.14. [Q9NZ43-3]
DR   Ensembl; ENST00000596136.5; ENSP00000473239.1; ENSG00000053501.14. [Q9NZ43-2]
DR   GeneID; 55850; -.
DR   KEGG; hsa:55850; -.
DR   MANE-Select; ENST00000263897.10; ENSP00000263897.4; NM_018467.4; NP_060937.2.
DR   UCSC; uc002nfo.3; human. [Q9NZ43-1]
DR   CTD; 55850; -.
DR   DisGeNET; 55850; -.
DR   GeneCards; USE1; -.
DR   HGNC; HGNC:30882; USE1.
DR   HPA; ENSG00000053501; Low tissue specificity.
DR   MIM; 610675; gene.
DR   neXtProt; NX_Q9NZ43; -.
DR   OpenTargets; ENSG00000053501; -.
DR   PharmGKB; PA162408692; -.
DR   VEuPathDB; HostDB:ENSG00000053501; -.
DR   eggNOG; KOG2678; Eukaryota.
DR   GeneTree; ENSGT00390000014361; -.
DR   HOGENOM; CLU_150739_0_0_1; -.
DR   InParanoid; Q9NZ43; -.
DR   OMA; CWLWLMI; -.
DR   OrthoDB; 1442410at2759; -.
DR   PhylomeDB; Q9NZ43; -.
DR   TreeFam; TF325006; -.
DR   PathwayCommons; Q9NZ43; -.
DR   Reactome; R-HSA-6811434; COPI-dependent Golgi-to-ER retrograde traffic.
DR   SignaLink; Q9NZ43; -.
DR   BioGRID-ORCS; 55850; 227 hits in 1083 CRISPR screens.
DR   ChiTaRS; USE1; human.
DR   GeneWiki; USE1; -.
DR   GenomeRNAi; 55850; -.
DR   Pharos; Q9NZ43; Tbio.
DR   PRO; PR:Q9NZ43; -.
DR   Proteomes; UP000005640; Chromosome 19.
DR   RNAct; Q9NZ43; protein.
DR   Bgee; ENSG00000053501; Expressed in tendon of biceps brachii and 193 other tissues.
DR   ExpressionAtlas; Q9NZ43; baseline and differential.
DR   Genevisible; Q9NZ43; HS.
DR   GO; GO:0005783; C:endoplasmic reticulum; IDA:HGNC-UCL.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; TAS:Reactome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031201; C:SNARE complex; IBA:GO_Central.
DR   GO; GO:0005484; F:SNAP receptor activity; IBA:GO_Central.
DR   GO; GO:0007041; P:lysosomal transport; ISS:HGNC-UCL.
DR   GO; GO:0030163; P:protein catabolic process; ISS:HGNC-UCL.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   GO; GO:0006890; P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum; IBA:GO_Central.
DR   GO; GO:0032940; P:secretion by cell; ISS:HGNC-UCL.
DR   InterPro; IPR019150; Vesicle_transport_protein_Use1.
DR   PANTHER; PTHR13050; PTHR13050; 1.
DR   Pfam; PF09753; Use1; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Coiled coil; Direct protein sequencing;
KW   Endoplasmic reticulum; ER-Golgi transport; Membrane; Protein transport;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..259
FT                   /note="Vesicle transport protein USE1"
FT                   /id="PRO_0000215579"
FT   TOPO_DOM        1..231
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        232..252
FT                   /note="Helical; Anchor for type IV membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        253..259
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   COILED          152..231
FT                   /evidence="ECO:0000255"
FT   VAR_SEQ         132..135
FT                   /note="EPEM -> GESP (in isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:14702039"
FT                   /id="VSP_012662"
FT   VAR_SEQ         136..259
FT                   /note="Missing (in isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:14702039"
FT                   /id="VSP_012663"
FT   VAR_SEQ         142..146
FT                   /note="GVAGS -> PCHTH (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_012664"
FT   VAR_SEQ         147..259
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_012665"
FT   VARIANT         154
FT                   /note="L -> S (in dbSNP:rs414528)"
FT                   /evidence="ECO:0000269|PubMed:12761501,
FT                   ECO:0000269|PubMed:15489334, ECO:0000269|Ref.3"
FT                   /id="VAR_021052"
FT   CONFLICT        38
FT                   /note="A -> V (in Ref. 4; AAH06005)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   259 AA;  29371 MW;  6538ED0B231AEC05 CRC64;
     MAASRLELNL VRLLSRCEAM AAEKRDPDEW RLEKYVGALE DMLQALKVHA SKPASEVINE
     YSWKVDFLKG MLQAEKLTSS SEKALANQFL APGRVPTTAR ERVPATKTVH LQSRARYTSE
     MRSELLGTDS AEPEMDVRKR TGVAGSQPVS EKQLAAELDL VLQRHQNLQE KLAEEMLGLA
     RSLKTNTLAA QSVIKKDNQT LSHSLKMADQ NLEKLKTESE RLEQHTQKSV NWLLWAMLII
     VCFIFISMIL FIRIMPKLK
//