ID T2R8_HUMAN STANDARD; PRT; 309 AA. AC Q9NYW2; DT 15-SEP-2003 (Rel. 42, Created) DT 15-SEP-2003 (Rel. 42, Last sequence update) DT 15-SEP-2003 (Rel. 42, Last annotation update) DE Taste receptor type 2 member 8 (T2R8) (Taste receptor family B member DE 5) (TRB5). GN TAS2R8. OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. OX NCBI_TaxID=9606; RN [1] RP SEQUENCE FROM N.A., AND TOPOLOGY. RX MEDLINE=20222571; PubMed=10761934; RA Adler E., Hoon M.A., Mueller K.L., Chandrashekar J., Ryba N.J.P., RA Zuker C.S.; RT "A novel family of mammalian taste receptors."; RL Cell 100:693-702(2000). RN [2] RP CHARACTERIZATION. RX MEDLINE=20222572; PubMed=10761935; RA Chandrashekar J., Mueller K.L., Hoon M.A., Adler E., Feng L., Guo W., RA Zuker C.S., Ryba N.J.; RT "T2Rs function as bitter taste receptors."; RL Cell 100:703-711(2000). RN [3] RP REVIEW. RX MEDLINE=22135574; PubMed=12139982; RA Montmayeur J.-P., Matsunami H.; RT "Receptors for bitter and sweet taste."; RL Curr. Opin. Neurobiol. 12:366-371(2002). RN [4] RP REVIEW. RX MEDLINE=21634924; PubMed=11696554; RA Margolskee R.F.; RT "Molecular mechanisms of bitter and sweet taste transduction."; RL J. Biol. Chem. 277:1-4(2002). RN [5] RP REVIEW. RX MEDLINE=22469025; PubMed=12581520; RA Zhang Y., Hoon M.A., Chandrashekar J., Mueller K.L., Cook B., Wu D., RA Zuker C.S., Ryba N.J.; RT "Coding of sweet, bitter, and umami tastes: different receptor cells RT sharing similar signaling pathways."; RL Cell 112:293-301(2003). CC -!- FUNCTION: Receptor that may play a role in the perception of CC bitterness and is gustducin-linked. May play a role in sensing the CC chemical composition of the gastrointestinal content. The activity CC of this receptor may stimulate alpha gustducin, mediate PLC-beta-2 CC activation and lead to the gating of TRPM5. CC -!- SUBCELLULAR LOCATION: Integral membrane protein. CC -!- TISSUE SPECIFICITY: Expressed in subsets of taste receptor cells CC of the tongue and palate epithelium and exclusively in gustducin- CC positive cells. CC -!- MISCELLANEOUS: Most taste cells may be activated by a limited CC number of bitter compounds; individual taste cells can CC discriminate among bitter stimuli. CC -!- SIMILARITY: Belongs to family T2R of G-protein coupled receptors. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AF227134; AAF43907.1; -. DR Genew; HGNC:14915; TAS2R8. DR MIM; 604794; -. DR InterPro; IPR000276; GPCR_Rhodpsn. DR Pfam; PF05296; TAS2R; 1. DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; UNKNOWN_1. KW Receptor; G-protein coupled receptor; Transmembrane. FT DOMAIN 1 7 EXTRACELLULAR (POTENTIAL). FT TRANSMEM 8 28 1 (POTENTIAL). FT DOMAIN 29 50 CYTOPLASMIC (POTENTIAL). FT TRANSMEM 51 71 2 (POTENTIAL). FT DOMAIN 72 82 EXTRACELLULAR (POTENTIAL). FT TRANSMEM 83 103 3 (POTENTIAL). FT DOMAIN 104 131 CYTOPLASMIC (POTENTIAL). FT TRANSMEM 132 152 4 (POTENTIAL). FT DOMAIN 153 184 EXTRACELLULAR (POTENTIAL). FT TRANSMEM 185 205 5 (POTENTIAL). FT DOMAIN 206 239 CYTOPLASMIC (POTENTIAL). FT TRANSMEM 240 260 6 (POTENTIAL). FT DOMAIN 261 266 EXTRACELLULAR (POTENTIAL). FT TRANSMEM 267 287 7 (POTENTIAL). FT DOMAIN 288 309 CYTOPLASMIC (POTENTIAL). FT CARBOHYD 167 167 N-LINKED (GLCNAC...) (POTENTIAL). SQ SEQUENCE 309 AA; 35877 MW; 5E3D94B726A52413 CRC64; MFSPADNIFI ILITGEFILG ILGNGYIALV NWIDWIKKKK ISTVDYILTN LVIARICLIS VMVVNGIVIV LNPDVYTKNK QQIVIFTFWT FANYLNMWIT TCLNVFYFLK IASSSHPLFL WLKWKIDMVV HWILLGCFAI SLLVSLIAAI VLSCDYRFHA IAKHKRNITE MFHVSKIPYF EPLTLFNLFA IVPFIVSLIS FFLLVRSLWR HTKQIKLYAT GSRDPSTEVH VRAIKTMTSF IFFFFLYYIS SILMTFSYLM TKYKLAVEFG EIAAILYPLG HSLILIVLNN KLRQTFVRML TCRKIACMI //