ID TA2R8_HUMAN Reviewed; 309 AA.
AC Q9NYW2; Q4KN29; Q645Y2;
DT 11-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 22-JUL-2008, entry version 55.
DE RecName: Full=Taste receptor type 2 member 8;
DE Short=T2R8;
DE AltName: Full=Taste receptor family B member 5;
DE Short=TRB5;
GN Name=TAS2R8;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TOPOLOGY.
RX MEDLINE=20222571; PubMed=10761934; DOI=10.1016/S0092-8674(00)80705-9;
RA Adler E., Hoon M.A., Mueller K.L., Chandrashekar J., Ryba N.J.P.,
RA Zuker C.S.;
RT "A novel family of mammalian taste receptors.";
RL Cell 100:693-702(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT VAL-308.
RX PubMed=15496549; DOI=10.1093/molbev/msi027;
RA Fischer A., Gilad Y., Man O., Paeaebo S.;
RT "Evolution of bitter taste receptors in humans and apes.";
RL Mol. Biol. Evol. 22:432-436(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP CHARACTERIZATION.
RX MEDLINE=20222572; PubMed=10761935; DOI=10.1016/S0092-8674(00)80706-0;
RA Chandrashekar J., Mueller K.L., Hoon M.A., Adler E., Feng L., Guo W.,
RA Zuker C.S., Ryba N.J.P.;
RT "T2Rs function as bitter taste receptors.";
RL Cell 100:703-711(2000).
RN [5]
RP REVIEW.
RX MEDLINE=22135574; PubMed=12139982; DOI=10.1016/S0959-4388(02)00345-8;
RA Montmayeur J.-P., Matsunami H.;
RT "Receptors for bitter and sweet taste.";
RL Curr. Opin. Neurobiol. 12:366-371(2002).
RN [6]
RP REVIEW.
RX MEDLINE=21634924; PubMed=11696554; DOI=10.1074/jbc.R100054200;
RA Margolskee R.F.;
RT "Molecular mechanisms of bitter and sweet taste transduction.";
RL J. Biol. Chem. 277:1-4(2002).
RN [7]
RP REVIEW.
RX MEDLINE=22469025; PubMed=12581520; DOI=10.1016/S0092-8674(03)00071-0;
RA Zhang Y., Hoon M.A., Chandrashekar J., Mueller K.L., Cook B., Wu D.,
RA Zuker C.S., Ryba N.J.;
RT "Coding of sweet, bitter, and umami tastes: different receptor cells
RT sharing similar signaling pathways.";
RL Cell 112:293-301(2003).
CC -!- FUNCTION: Receptor that may play a role in the perception of
CC bitterness and is gustducin-linked. May play a role in sensing the
CC chemical composition of the gastrointestinal content. The activity
CC of this receptor may stimulate alpha gustducin, mediate PLC-beta-2
CC activation and lead to the gating of TRPM5.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Expressed in subsets of taste receptor cells
CC of the tongue and palate epithelium and exclusively in gustducin-
CC positive cells.
CC -!- MISCELLANEOUS: Most taste cells may be activated by a limited
CC number of bitter compounds; individual taste cells can
CC discriminate among bitter stimuli.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor T2R family.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF227134; AAF43907.1; -; Genomic_DNA.
DR EMBL; AY724935; AAU21137.1; -; Genomic_DNA.
DR EMBL; BC069084; AAH69084.1; -; mRNA.
DR EMBL; BC069551; AAH69551.1; -; mRNA.
DR EMBL; BC096701; AAH96701.1; -; mRNA.
DR EMBL; BC096735; AAH96735.1; -; mRNA.
DR EMBL; BC098353; AAH98353.1; -; mRNA.
DR RefSeq; NP_076407.1; -.
DR UniGene; Hs.533755; -.
DR PhosphoSite; Q9NYW2; -.
DR Ensembl; ENSG00000121314; Homo sapiens.
DR GeneID; 50836; -.
DR KEGG; hsa:50836; -.
DR H-InvDB; HIX0036818; -.
DR HGNC; HGNC:14915; TAS2R8.
DR MIM; 604794; gene.
DR PharmGKB; PA37926; -.
DR HOGENOM; Q9NYW2; -.
DR HOVERGEN; Q9NYW2; -.
DR LinkHub; Q9NYW2; -.
DR ArrayExpress; Q9NYW2; -.
DR CleanEx; HS_TAS2R8; -.
DR GermOnline; ENSG00000121314; Homo sapiens.
DR GO; GO:0016021; C:integral to membrane; NAS:UniProtKB.
DR GO; GO:0008527; F:taste receptor activity; TAS:UniProtKB.
DR InterPro; IPR017452; GPCR_Rhodpsn_supfam.
DR InterPro; IPR007960; TAS2_rcpt.
DR Pfam; PF05296; TAS2R; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; UNKNOWN_1.
PE 1: Evidence at protein level;
KW G-protein coupled receptor; Glycoprotein; Membrane; Polymorphism;
KW Receptor; Sensory transduction; Taste; Transducer; Transmembrane.
FT CHAIN 1 309 Taste receptor type 2 member 8.
FT /FTId=PRO_0000082227.
FT TOPO_DOM 1 7 Extracellular (Potential).
FT TRANSMEM 8 28 1 (Potential).
FT TOPO_DOM 29 50 Cytoplasmic (Potential).
FT TRANSMEM 51 71 2 (Potential).
FT TOPO_DOM 72 82 Extracellular (Potential).
FT TRANSMEM 83 103 3 (Potential).
FT TOPO_DOM 104 131 Cytoplasmic (Potential).
FT TRANSMEM 132 152 4 (Potential).
FT TOPO_DOM 153 184 Extracellular (Potential).
FT TRANSMEM 185 205 5 (Potential).
FT TOPO_DOM 206 239 Cytoplasmic (Potential).
FT TRANSMEM 240 260 6 (Potential).
FT TOPO_DOM 261 266 Extracellular (Potential).
FT TRANSMEM 267 287 7 (Potential).
FT TOPO_DOM 288 309 Cytoplasmic (Potential).
FT CARBOHYD 167 167 N-linked (GlcNAc...) (Potential).
FT VARIANT 308 308 M -> V (in dbSNP:rs2537817).
FT /FTId=VAR_024186.
SQ SEQUENCE 309 AA; 35877 MW; 5E3D94B726A52413 CRC64;
MFSPADNIFI ILITGEFILG ILGNGYIALV NWIDWIKKKK ISTVDYILTN LVIARICLIS
VMVVNGIVIV LNPDVYTKNK QQIVIFTFWT FANYLNMWIT TCLNVFYFLK IASSSHPLFL
WLKWKIDMVV HWILLGCFAI SLLVSLIAAI VLSCDYRFHA IAKHKRNITE MFHVSKIPYF
EPLTLFNLFA IVPFIVSLIS FFLLVRSLWR HTKQIKLYAT GSRDPSTEVH VRAIKTMTSF
IFFFFLYYIS SILMTFSYLM TKYKLAVEFG EIAAILYPLG HSLILIVLNN KLRQTFVRML
TCRKIACMI
//