ID TA2R8_HUMAN Reviewed; 309 AA. AC Q9NYW2; Q4KN29; Q645Y2; DT 11-APR-2003, integrated into UniProtKB/Swiss-Prot. DT 01-OCT-2000, sequence version 1. DT 02-OCT-2024, entry version 170. DE RecName: Full=Taste receptor type 2 member 8; DE Short=T2R8; DE AltName: Full=Taste receptor family B member 5; DE Short=TRB5; GN Name=TAS2R8; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; OC Homo. OX NCBI_TaxID=9606; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TOPOLOGY. RX PubMed=10761934; DOI=10.1016/s0092-8674(00)80705-9; RA Adler E., Hoon M.A., Mueller K.L., Chandrashekar J., Ryba N.J.P., RA Zuker C.S.; RT "A novel family of mammalian taste receptors."; RL Cell 100:693-702(2000). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT VAL-308. RX PubMed=15496549; DOI=10.1093/molbev/msi027; RA Fischer A., Gilad Y., Man O., Paeaebo S.; RT "Evolution of bitter taste receptors in humans and apes."; RL Mol. Biol. Evol. 22:432-436(2005). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA project: RT the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [4] RP CHARACTERIZATION. RX PubMed=10761935; DOI=10.1016/s0092-8674(00)80706-0; RA Chandrashekar J., Mueller K.L., Hoon M.A., Adler E., Feng L., Guo W., RA Zuker C.S., Ryba N.J.P.; RT "T2Rs function as bitter taste receptors."; RL Cell 100:703-711(2000). RN [5] RP REVIEW. RX PubMed=12139982; DOI=10.1016/s0959-4388(02)00345-8; RA Montmayeur J.-P., Matsunami H.; RT "Receptors for bitter and sweet taste."; RL Curr. Opin. Neurobiol. 12:366-371(2002). RN [6] RP REVIEW. RX PubMed=11696554; DOI=10.1074/jbc.r100054200; RA Margolskee R.F.; RT "Molecular mechanisms of bitter and sweet taste transduction."; RL J. Biol. Chem. 277:1-4(2002). RN [7] RP REVIEW. RX PubMed=12581520; DOI=10.1016/s0092-8674(03)00071-0; RA Zhang Y., Hoon M.A., Chandrashekar J., Mueller K.L., Cook B., Wu D., RA Zuker C.S., Ryba N.J.; RT "Coding of sweet, bitter, and umami tastes: different receptor cells RT sharing similar signaling pathways."; RL Cell 112:293-301(2003). CC -!- FUNCTION: Receptor that may play a role in the perception of bitterness CC and is gustducin-linked. May play a role in sensing the chemical CC composition of the gastrointestinal content. The activity of this CC receptor may stimulate alpha gustducin, mediate PLC-beta-2 activation CC and lead to the gating of TRPM5. CC -!- INTERACTION: CC Q9NYW2; Q86UT5-3: NHERF4; NbExp=3; IntAct=EBI-12092809, EBI-12092811; CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. CC -!- TISSUE SPECIFICITY: Expressed in subsets of taste receptor cells of the CC tongue and palate epithelium and exclusively in gustducin-positive CC cells. CC -!- MISCELLANEOUS: Most taste cells may be activated by a limited number of CC bitter compounds; individual taste cells can discriminate among bitter CC stimuli. CC -!- SIMILARITY: Belongs to the G-protein coupled receptor T2R family. CC {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AF227134; AAF43907.1; -; Genomic_DNA. DR EMBL; AY724935; AAU21137.1; -; Genomic_DNA. DR EMBL; BC069084; AAH69084.1; -; mRNA. DR EMBL; BC069551; AAH69551.1; -; mRNA. DR EMBL; BC096701; AAH96701.1; -; mRNA. DR EMBL; BC096735; AAH96735.1; -; mRNA. DR EMBL; BC098353; AAH98353.1; -; mRNA. DR CCDS; CCDS8632.1; -. DR RefSeq; NP_076407.1; NM_023918.1. DR AlphaFoldDB; Q9NYW2; -. DR SMR; Q9NYW2; -. DR BioGRID; 119146; 4. DR IntAct; Q9NYW2; 4. DR STRING; 9606.ENSP00000240615; -. DR BindingDB; Q9NYW2; -. DR ChEMBL; CHEMBL3988599; -. DR DrugCentral; Q9NYW2; -. DR GuidetoPHARMACOLOGY; 664; -. DR GlyCosmos; Q9NYW2; 1 site, No reported glycans. DR GlyGen; Q9NYW2; 1 site. DR iPTMnet; Q9NYW2; -. DR PhosphoSitePlus; Q9NYW2; -. DR BioMuta; TAS2R8; -. DR DMDM; 29839662; -. DR MassIVE; Q9NYW2; -. DR PaxDb; 9606-ENSP00000240615; -. DR PeptideAtlas; Q9NYW2; -. DR Antibodypedia; 23396; 50 antibodies from 15 providers. DR DNASU; 50836; -. DR Ensembl; ENST00000240615.3; ENSP00000240615.2; ENSG00000121314.3. DR Ensembl; ENST00000572823.1; ENSP00000461158.1; ENSG00000272712.1. DR Ensembl; ENST00000610444.2; ENSP00000484163.1; ENSG00000277316.2. DR GeneID; 50836; -. DR KEGG; hsa:50836; -. DR MANE-Select; ENST00000240615.3; ENSP00000240615.2; NM_023918.3; NP_076407.1. DR UCSC; uc010shh.3; human. DR AGR; HGNC:14915; -. DR CTD; 50836; -. DR DisGeNET; 50836; -. DR GeneCards; TAS2R8; -. DR HGNC; HGNC:14915; TAS2R8. DR HPA; ENSG00000121314; Not detected. DR MIM; 604794; gene. DR neXtProt; NX_Q9NYW2; -. DR OpenTargets; ENSG00000121314; -. DR PharmGKB; PA37926; -. DR VEuPathDB; HostDB:ENSG00000121314; -. DR eggNOG; ENOG502SKRK; Eukaryota. DR GeneTree; ENSGT01100000263477; -. DR HOGENOM; CLU_072337_3_0_1; -. DR InParanoid; Q9NYW2; -. DR OMA; NWIDWIK; -. DR OrthoDB; 5306466at2759; -. DR PhylomeDB; Q9NYW2; -. DR TreeFam; TF335891; -. DR PathwayCommons; Q9NYW2; -. DR Reactome; R-HSA-418594; G alpha (i) signalling events. DR Reactome; R-HSA-420499; Class C/3 (Metabotropic glutamate/pheromone receptors). DR Reactome; R-HSA-9717207; Sensory perception of sweet, bitter, and umami (glutamate) taste. DR SignaLink; Q9NYW2; -. DR BioGRID-ORCS; 50836; 13 hits in 1137 CRISPR screens. DR GeneWiki; TAS2R8; -. DR GenomeRNAi; 50836; -. DR Pharos; Q9NYW2; Tchem. DR PRO; PR:Q9NYW2; -. DR Proteomes; UP000005640; Chromosome 12. DR RNAct; Q9NYW2; protein. DR Bgee; ENSG00000121314; Expressed in cortical plate and 6 other cell types or tissues. DR GO; GO:0016020; C:membrane; IBA:GO_Central. DR GO; GO:0005886; C:plasma membrane; TAS:Reactome. DR GO; GO:0033038; F:bitter taste receptor activity; IDA:UniProtKB. DR GO; GO:0004930; F:G protein-coupled receptor activity; IEA:UniProtKB-KW. DR GO; GO:0008527; F:taste receptor activity; TAS:UniProtKB. DR GO; GO:0001580; P:detection of chemical stimulus involved in sensory perception of bitter taste; IDA:UniProtKB. DR CDD; cd15022; 7tm_TAS2R8; 1. DR Gene3D; 1.20.1070.10; Rhodopsin 7-helix transmembrane proteins; 1. DR InterPro; IPR017452; GPCR_Rhodpsn_7TM. DR InterPro; IPR007960; TAS2R. DR PANTHER; PTHR11394; TASTE RECEPTOR TYPE 2; 1. DR PANTHER; PTHR11394:SF31; TASTE RECEPTOR TYPE 2 MEMBER 8; 1. DR Pfam; PF05296; TAS2R; 1. DR SUPFAM; SSF81321; Family A G protein-coupled receptor-like; 1. DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1. PE 1: Evidence at protein level; KW G-protein coupled receptor; Glycoprotein; Membrane; Receptor; KW Reference proteome; Sensory transduction; Taste; Transducer; Transmembrane; KW Transmembrane helix. FT CHAIN 1..309 FT /note="Taste receptor type 2 member 8" FT /id="PRO_0000082227" FT TOPO_DOM 1..7 FT /note="Extracellular" FT /evidence="ECO:0000255" FT TRANSMEM 8..28 FT /note="Helical; Name=1" FT /evidence="ECO:0000255" FT TOPO_DOM 29..50 FT /note="Cytoplasmic" FT /evidence="ECO:0000255" FT TRANSMEM 51..71 FT /note="Helical; Name=2" FT /evidence="ECO:0000255" FT TOPO_DOM 72..82 FT /note="Extracellular" FT /evidence="ECO:0000255" FT TRANSMEM 83..103 FT /note="Helical; Name=3" FT /evidence="ECO:0000255" FT TOPO_DOM 104..131 FT /note="Cytoplasmic" FT /evidence="ECO:0000255" FT TRANSMEM 132..152 FT /note="Helical; Name=4" FT /evidence="ECO:0000255" FT TOPO_DOM 153..184 FT /note="Extracellular" FT /evidence="ECO:0000255" FT TRANSMEM 185..205 FT /note="Helical; Name=5" FT /evidence="ECO:0000255" FT TOPO_DOM 206..239 FT /note="Cytoplasmic" FT /evidence="ECO:0000255" FT TRANSMEM 240..260 FT /note="Helical; Name=6" FT /evidence="ECO:0000255" FT TOPO_DOM 261..266 FT /note="Extracellular" FT /evidence="ECO:0000255" FT TRANSMEM 267..287 FT /note="Helical; Name=7" FT /evidence="ECO:0000255" FT TOPO_DOM 288..309 FT /note="Cytoplasmic" FT /evidence="ECO:0000255" FT CARBOHYD 167 FT /note="N-linked (GlcNAc...) asparagine" FT /evidence="ECO:0000255" FT VARIANT 308 FT /note="M -> V (in dbSNP:rs2537817)" FT /evidence="ECO:0000269|PubMed:15496549" FT /id="VAR_024186" SQ SEQUENCE 309 AA; 35877 MW; 5E3D94B726A52413 CRC64; MFSPADNIFI ILITGEFILG ILGNGYIALV NWIDWIKKKK ISTVDYILTN LVIARICLIS VMVVNGIVIV LNPDVYTKNK QQIVIFTFWT FANYLNMWIT TCLNVFYFLK IASSSHPLFL WLKWKIDMVV HWILLGCFAI SLLVSLIAAI VLSCDYRFHA IAKHKRNITE MFHVSKIPYF EPLTLFNLFA IVPFIVSLIS FFLLVRSLWR HTKQIKLYAT GSRDPSTEVH VRAIKTMTSF IFFFFLYYIS SILMTFSYLM TKYKLAVEFG EIAAILYPLG HSLILIVLNN KLRQTFVRML TCRKIACMI //