ID TA2R8_HUMAN Reviewed; 309 AA. AC Q9NYW2; Q4KN29; Q645Y2; DT 11-APR-2003, integrated into UniProtKB/Swiss-Prot. DT 01-OCT-2000, sequence version 1. DT 07-JUN-2017, entry version 130. DE RecName: Full=Taste receptor type 2 member 8; DE Short=T2R8; DE AltName: Full=Taste receptor family B member 5; DE Short=TRB5; GN Name=TAS2R8; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; OC Catarrhini; Hominidae; Homo. OX NCBI_TaxID=9606; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TOPOLOGY. RX PubMed=10761934; DOI=10.1016/S0092-8674(00)80705-9; RA Adler E., Hoon M.A., Mueller K.L., Chandrashekar J., Ryba N.J.P., RA Zuker C.S.; RT "A novel family of mammalian taste receptors."; RL Cell 100:693-702(2000). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT VAL-308. RX PubMed=15496549; DOI=10.1093/molbev/msi027; RA Fischer A., Gilad Y., Man O., Paeaebo S.; RT "Evolution of bitter taste receptors in humans and apes."; RL Mol. Biol. Evol. 22:432-436(2005). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA RT project: the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [4] RP CHARACTERIZATION. RX PubMed=10761935; DOI=10.1016/S0092-8674(00)80706-0; RA Chandrashekar J., Mueller K.L., Hoon M.A., Adler E., Feng L., Guo W., RA Zuker C.S., Ryba N.J.P.; RT "T2Rs function as bitter taste receptors."; RL Cell 100:703-711(2000). RN [5] RP REVIEW. RX PubMed=12139982; DOI=10.1016/S0959-4388(02)00345-8; RA Montmayeur J.-P., Matsunami H.; RT "Receptors for bitter and sweet taste."; RL Curr. Opin. Neurobiol. 12:366-371(2002). RN [6] RP REVIEW. RX PubMed=11696554; DOI=10.1074/jbc.R100054200; RA Margolskee R.F.; RT "Molecular mechanisms of bitter and sweet taste transduction."; RL J. Biol. Chem. 277:1-4(2002). RN [7] RP REVIEW. RX PubMed=12581520; DOI=10.1016/S0092-8674(03)00071-0; RA Zhang Y., Hoon M.A., Chandrashekar J., Mueller K.L., Cook B., Wu D., RA Zuker C.S., Ryba N.J.; RT "Coding of sweet, bitter, and umami tastes: different receptor cells RT sharing similar signaling pathways."; RL Cell 112:293-301(2003). CC -!- FUNCTION: Receptor that may play a role in the perception of CC bitterness and is gustducin-linked. May play a role in sensing the CC chemical composition of the gastrointestinal content. The activity CC of this receptor may stimulate alpha gustducin, mediate PLC-beta-2 CC activation and lead to the gating of TRPM5. CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. CC -!- TISSUE SPECIFICITY: Expressed in subsets of taste receptor cells CC of the tongue and palate epithelium and exclusively in gustducin- CC positive cells. CC -!- MISCELLANEOUS: Most taste cells may be activated by a limited CC number of bitter compounds; individual taste cells can CC discriminate among bitter stimuli. CC -!- SIMILARITY: Belongs to the G-protein coupled receptor T2R family. CC {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AF227134; AAF43907.1; -; Genomic_DNA. DR EMBL; AY724935; AAU21137.1; -; Genomic_DNA. DR EMBL; BC069084; AAH69084.1; -; mRNA. DR EMBL; BC069551; AAH69551.1; -; mRNA. DR EMBL; BC096701; AAH96701.1; -; mRNA. DR EMBL; BC096735; AAH96735.1; -; mRNA. DR EMBL; BC098353; AAH98353.1; -; mRNA. DR CCDS; CCDS8632.1; -. DR RefSeq; NP_076407.1; NM_023918.1. DR UniGene; Hs.533755; -. DR ProteinModelPortal; Q9NYW2; -. DR BioGrid; 119146; 1. DR IntAct; Q9NYW2; 1. DR BindingDB; Q9NYW2; -. DR iPTMnet; Q9NYW2; -. DR PhosphoSitePlus; Q9NYW2; -. DR BioMuta; TAS2R8; -. DR DMDM; 29839662; -. DR PaxDb; Q9NYW2; -. DR PeptideAtlas; Q9NYW2; -. DR PRIDE; Q9NYW2; -. DR DNASU; 50836; -. DR Ensembl; ENST00000240615; ENSP00000240615; ENSG00000121314. DR Ensembl; ENST00000572823; ENSP00000461158; ENSG00000272712. DR Ensembl; ENST00000610444; ENSP00000484163; ENSG00000277316. DR GeneID; 50836; -. DR KEGG; hsa:50836; -. DR UCSC; uc010shh.3; human. DR CTD; 50836; -. DR GeneCards; TAS2R8; -. DR HGNC; HGNC:14915; TAS2R8. DR MIM; 604794; gene. DR neXtProt; NX_Q9NYW2; -. DR OpenTargets; ENSG00000121314; -. DR PharmGKB; PA37926; -. DR eggNOG; ENOG410IFAT; Eukaryota. DR eggNOG; ENOG410YQIB; LUCA. DR GeneTree; ENSGT00760000119096; -. DR HOGENOM; HOG000234595; -. DR HOVERGEN; HBG060698; -. DR InParanoid; Q9NYW2; -. DR KO; K08474; -. DR OMA; NWIDWIK; -. DR OrthoDB; EOG091G0G0Q; -. DR PhylomeDB; Q9NYW2; -. DR TreeFam; TF335891; -. DR Reactome; R-HSA-418594; G alpha (i) signalling events. DR Reactome; R-HSA-420499; Class C/3 (Metabotropic glutamate/pheromone receptors). DR GeneWiki; TAS2R8; -. DR GenomeRNAi; 50836; -. DR PRO; PR:Q9NYW2; -. DR Proteomes; UP000005640; Chromosome 12. DR CleanEx; HS_TAS2R8; -. DR Genevisible; Q9NYW2; HS. DR GO; GO:0016021; C:integral component of membrane; IC:UniProtKB. DR GO; GO:0005886; C:plasma membrane; TAS:Reactome. DR GO; GO:0033038; F:bitter taste receptor activity; IDA:UniProtKB. DR GO; GO:0004930; F:G-protein coupled receptor activity; IEA:UniProtKB-KW. DR GO; GO:0008527; F:taste receptor activity; TAS:UniProtKB. DR GO; GO:0001580; P:detection of chemical stimulus involved in sensory perception of bitter taste; IDA:UniProtKB. DR InterPro; IPR017452; GPCR_Rhodpsn_7TM. DR InterPro; IPR007960; T2R. DR PANTHER; PTHR11394; PTHR11394; 1. DR Pfam; PF05296; TAS2R; 1. DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1. PE 1: Evidence at protein level; KW Complete proteome; G-protein coupled receptor; Glycoprotein; Membrane; KW Polymorphism; Receptor; Reference proteome; Sensory transduction; KW Taste; Transducer; Transmembrane; Transmembrane helix. FT CHAIN 1 309 Taste receptor type 2 member 8. FT /FTId=PRO_0000082227. FT TOPO_DOM 1 7 Extracellular. {ECO:0000255}. FT TRANSMEM 8 28 Helical; Name=1. {ECO:0000255}. FT TOPO_DOM 29 50 Cytoplasmic. {ECO:0000255}. FT TRANSMEM 51 71 Helical; Name=2. {ECO:0000255}. FT TOPO_DOM 72 82 Extracellular. {ECO:0000255}. FT TRANSMEM 83 103 Helical; Name=3. {ECO:0000255}. FT TOPO_DOM 104 131 Cytoplasmic. {ECO:0000255}. FT TRANSMEM 132 152 Helical; Name=4. {ECO:0000255}. FT TOPO_DOM 153 184 Extracellular. {ECO:0000255}. FT TRANSMEM 185 205 Helical; Name=5. {ECO:0000255}. FT TOPO_DOM 206 239 Cytoplasmic. {ECO:0000255}. FT TRANSMEM 240 260 Helical; Name=6. {ECO:0000255}. FT TOPO_DOM 261 266 Extracellular. {ECO:0000255}. FT TRANSMEM 267 287 Helical; Name=7. {ECO:0000255}. FT TOPO_DOM 288 309 Cytoplasmic. {ECO:0000255}. FT CARBOHYD 167 167 N-linked (GlcNAc...) asparagine. FT {ECO:0000255}. FT VARIANT 308 308 M -> V (in dbSNP:rs2537817). FT {ECO:0000269|PubMed:15496549}. FT /FTId=VAR_024186. SQ SEQUENCE 309 AA; 35877 MW; 5E3D94B726A52413 CRC64; MFSPADNIFI ILITGEFILG ILGNGYIALV NWIDWIKKKK ISTVDYILTN LVIARICLIS VMVVNGIVIV LNPDVYTKNK QQIVIFTFWT FANYLNMWIT TCLNVFYFLK IASSSHPLFL WLKWKIDMVV HWILLGCFAI SLLVSLIAAI VLSCDYRFHA IAKHKRNITE MFHVSKIPYF EPLTLFNLFA IVPFIVSLIS FFLLVRSLWR HTKQIKLYAT GSRDPSTEVH VRAIKTMTSF IFFFFLYYIS SILMTFSYLM TKYKLAVEFG EIAAILYPLG HSLILIVLNN KLRQTFVRML TCRKIACMI //