ID PSMF1_ARATH Reviewed; 302 AA. AC Q9M330; DT 26-SEP-2001, integrated into UniProtKB/Swiss-Prot. DT 01-OCT-2000, sequence version 1. DT 06-JUL-2016, entry version 82. DE RecName: Full=Probable proteasome inhibitor; GN OrderedLocusNames=At3g53970; ORFNames=F5K20_270; OS Arabidopsis thaliana (Mouse-ear cress). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae; OC Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; OC Arabidopsis. OX NCBI_TaxID=3702; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Columbia; RX PubMed=11130713; DOI=10.1038/35048706; RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., RA Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., RA Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., RA De Simone V., Choisne N., Artiguenave F., Robert C., Brottier P., RA Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., RA Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., RA Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., RA Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., RA Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., RA Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., RA Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., RA Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., RA Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., RA Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., RA Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., RA Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., RA Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., RA Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., RA Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., RA Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., RA Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., RA Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., RA Watanabe A., Yamada M., Yasuda M., Tabata S.; RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis RT thaliana."; RL Nature 408:820-822(2000). RN [2] RP GENOME REANNOTATION. RC STRAIN=cv. Columbia; RG The Arabidopsis Information Resource (TAIR); RL Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases. RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=cv. Columbia; RX PubMed=14593172; DOI=10.1126/science.1088305; RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., RA Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., RA Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G., RA Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., RA Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., RA Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., RA Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., RA Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., RA Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., RA Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., RA Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., RA Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.; RT "Empirical analysis of transcriptional activity in the Arabidopsis RT genome."; RL Science 302:842-846(2003). RN [4] RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS RP SPECTROMETRY [LARGE SCALE ANALYSIS]. RX PubMed=22223895; DOI=10.1074/mcp.M111.015131; RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., RA Meinnel T., Giglione C.; RT "Comparative large-scale characterisation of plant vs. mammal proteins RT reveals similar and idiosyncratic N-alpha acetylation features."; RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012). CC -!- FUNCTION: Could play an important role in control of proteasome CC function. Inhibits the hydrolysis of protein and peptide CC substrates by the 20S proteasome (By similarity). {ECO:0000250}. CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=1; CC Comment=A number of isoforms are produced. According to EST CC sequences.; CC Name=1; CC IsoId=Q9M330-1; Sequence=Displayed; CC -!- SIMILARITY: Belongs to the proteasome inhibitor PI31 family. CC {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AL132960; CAB88359.1; -; Genomic_DNA. DR EMBL; CP002686; AEE79166.1; -; Genomic_DNA. DR EMBL; AY063998; AAL36354.1; -; mRNA. DR EMBL; AY117182; AAM51257.1; -; mRNA. DR PIR; T45937; T45937. DR RefSeq; NP_190965.1; NM_115257.5. [Q9M330-1] DR UniGene; At.1410; -. DR ProteinModelPortal; Q9M330; -. DR SMR; Q9M330; 17-148. DR iPTMnet; Q9M330; -. DR PaxDb; Q9M330; -. DR PRIDE; Q9M330; -. DR EnsemblPlants; AT3G53970.1; AT3G53970.1; AT3G53970. [Q9M330-1] DR GeneID; 824564; -. DR KEGG; ath:AT3G53970; -. DR TAIR; AT3G53970; -. DR eggNOG; KOG4761; Eukaryota. DR eggNOG; ENOG4111GK4; LUCA. DR HOGENOM; HOG000006033; -. DR InParanoid; Q9M330; -. DR KO; K06700; -. DR OMA; LRYEYKD; -. DR PhylomeDB; Q9M330; -. DR PRO; PR:Q9M330; -. DR Proteomes; UP000006548; Chromosome 3. DR ExpressionAtlas; Q9M330; baseline and differential. DR Genevisible; Q9M330; AT. DR GO; GO:0000502; C:proteasome complex; IEA:UniProtKB-KW. DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IBA:GO_Central. DR InterPro; IPR021625; PI31_Prot_N. DR Pfam; PF11566; PI31_Prot_N; 1. PE 1: Evidence at protein level; KW Acetylation; Alternative splicing; Complete proteome; Proteasome; KW Reference proteome. FT INIT_MET 1 1 Removed. {ECO:0000244|PubMed:22223895}. FT CHAIN 2 302 Probable proteasome inhibitor. FT /FTId=PRO_0000220925. FT COMPBIAS 155 302 Pro-rich. FT MOD_RES 2 2 N-acetylalanine. FT {ECO:0000244|PubMed:22223895}. SQ SEQUENCE 302 AA; 32117 MW; 1DE8CFB5E55914E2 CRC64; MANSQTVMAM IRLARPPFRN NHDKVAFAIH SSFVASGYIL TATGRPAFAD EALSSSSQND VGIEGWNEFE GEYAFVYANP KKGSKKILVK CLAMDDKLLV DAIADGGAEP AHLEIKVGDY AEESNEGDYS AQFKNLDKLV TDLQSEIIDK LDGKPKPVAS RAQSSSETNE EPRYYDDTPN PLGPQIHPSG VVVPPIPGNG GYSDLFPGPG AGMYPGRGGF GDGSMLVGPT DPRFFPFGDG SDRPGFMGPP HPGMPPPGAR FDPYGPPGVP GFEPGRFTRQ PPRGPGGGHP DLEHFPGGSD FI //